LOCUS       pDM043        3403 bp DNA     circular SYN 05-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Domenico Modaffari
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3403
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     primer_bind     378..396
                     /label=M13 fwd
                     /note="color: black; sequence: TGTAAAACGACGGCCAGTG; added: 
                     2023-04-26"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             418..1104
                     /codon_start=1
                     /label=mScarlet3
                     /note="A. nidulans codon-adjusted"
                     /note="color: #ff0000"
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     CDS             1105..1134
                     /codon_start=1
                     /label=VinnyLinker
                     /note="color: #00ffff"
                     /translation="PDVLELAAAQ"
     primer_bind     complement(1182..1198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(1195..1217)
                     /label=M13 rev
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG; 
                     added: 2023-04-26"
     protein_bind    1206..1222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(1230..1236,1237..1254,1255..1260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 1230 .. 1236 / #ffffff / -10
                        2: 1237 .. 1254 / #ffffff
                        3: 1255 .. 1260 / #ffffff / -35"
     rep_origin      complement(1584..2172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(2343..3134,3135..3203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2343 .. 3134 / #ccffcc
                        2: 3135 .. 3203 / #ccffcc / signal sequence
                     Cleavage site after base 3134"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3204..3308)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgggatg
      421 gactccaccg aggccgtcat caaggagttt atgcgtttta aggttcacat ggaaggttct
      481 atgaacggtc acgagttcga gatcgagggt gagggtgagg gtcgccccta cgagggtacc
      541 cagaccgcca agttacgtgt tactaaaggt ggtcctttac ctttctcctg ggacatcctc
      601 tctcctcagt tcatgtacgg ttcccgcgcc ttcaccaagc accccgctga tattcccgat
      661 tattggaagc agtcctttcc cgagggtttc aagtgggagc gcgtcatgaa cttcgaagat
      721 ggtggtgccg tctctgttgc ccaagatact tcccttgagg acggtaccct catctacaag
      781 gtcaagctcc gcggtaccaa ttttcctcct gatggtcccg tcatgcagaa gaagaccatg
      841 ggttgggagg cctccaccga acgtctctac cctgaggatg tcgttcttaa aggtgacatc
      901 aagatggccc ttcgccttaa ggacggtggt cgctacctcg ctgacttcaa gaccacctac
      961 cgtgctaaaa agcccgttca gatgcctggt gccttcaaca tcgaccgcaa gctcgacatc
     1021 acctcccaca acgaggacta caccgtcgtc gaacaatatg agcgttccgt tgctcgtcat
     1081 tctaccggtg gttctggtgg ttcccctgac gtcctcgagc tcgctgctgc tcaggatcct
     1141 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
