LOCUS       pDM071        3445 bp DNA     circular SYN 05-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Domenico Modaffari
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3445
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             423..1154
                     /codon_start=1
                     /label=mCardinal
                     /label=mCardinal (A. nid)
                     /note="A. nidulans codon-adjusted"
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             1155..1175
                     /codon_start=1
                     /label=Reverse RIPGLIN
                     /label=@cds
                     /note="/color=#355c87"
                     /note="/role=constraint"
                     /note="color: #355c87"
                     /translation="NILGPIR"
     primer_bind     complement(1224..1240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      complement(1626..2214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(2385..3176,3177..3245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2385 .. 3176 / #ccffcc
                        2: 3177 .. 3245 / #ccffcc / signal sequence
                     Cleavage site after base 3176"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3246..3350)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 ccatggtctc caagggtgag gagctcatca aggagaacat gcacatgaag ctctacatgg
      481 agggtaccgt caacaaccac cacttcaagt gcaccaccga aggtgagggt aagccctacg
      541 agggtaccca gacccagcgc atcaaagttg tcgaaggtgg tcccctccct ttcgctttcg
      601 acatcctcgc cacctgcttc atgtacggtt ccaagacctt catcaaccac acccagggta
      661 tccccgactt cttcaagcag tccttccccg agggtttcac ctgggagcgc gttactacct
      721 acgaggatgg tggtgtcctc actgtcaccc aagacacctc ccttcaggac ggttgcctca
      781 tctacaacgt caagctccgc ggtgtcaact tcccctccaa cggtcccgtc atgcagaaga
      841 agacccttgg ttgggaagct actactgaga ccctttaccc cgccgacggt ggtctcgagg
      901 gtcgttgtga catggcctta aagctcgttg gtggtggtca cttacactgc aacctcaaga
      961 ccacctaccg ctccaagaag cccgccaaga acctcaagat gcccggtgtc tactttgttg
     1021 accgtcgcct tgagcgcatc aaggaggccg acaacgagac ctacgttgaa caacacgagg
     1081 tcgccgttgc tcgctactgc gacctccctt ctaagctcgg tcacaagctc aacggtatgg
     1141 acgagctcta caagaacatc ctcggtccca tccgcggatc ctctagagtc gacctgcagg
     1201 catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1321 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1801 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2041 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3361 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     3421 aggcgtatca cgaggccctt tcgtc
//
