LOCUS       pHAGE-FKBP-GFP-S        9720 bp DNA     circular SYN 11-FEB-2024
DEFINITION  stably express GFP-tagged NDP52 for CID induced mitophagy.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Vargas JNS, Wang C, Bunker E, Hao L, Maric D, Schiavo G, Randow F, 
            Youle RJ
  TITLE     Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during 
            Selective Autophagy.
  JOURNAL   Mol Cell. 2019 Apr 18;74(2):347-362.e6. doi: 
            10.1016/j.molcel.2019.02.010. Epub 2019 Mar 7.
  PUBMED    30853401
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 17, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.02.010"; journalName: "Mol Cell"; date: 
            "2019-04-18- 18"; volume: "74"; issue: "2"; pages: "347-362.e6"
COMMENT     Sequence Label: pHAGE-FKBP-GFP-NDP52
FEATURES             Location/Qualifiers
     source          1..9720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1948..1974
                     /label=SMP3608
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2180..2201
                     /label=SMP2956
     primer_bind     2191..2210
                     /label=SMP2937
     primer_bind     2194..2214
                     /label=SMP2954
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2376..2397)
                     /label=SMP1803
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2564..2579)
                     /label=SMP2619
     primer_bind     2643..2664
                     /label=SMP2400
     primer_bind     2646..2665
                     /label=EGFP-N-for
     primer_bind     2683..2701
                     /label=SMP443
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2702..2718
                     /label=IRES-for
     primer_bind     2717..2737
                     /label=SMP736
     primer_bind     2728..2747
                     /label=pTRE-for
     primer_bind     complement(2732..2766)
                     /label=SMP4739
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     2735..2755
                     /label=SMP2952
     primer_bind     2735..2752
                     /label=CEP4-for
     primer_bind     complement(2745..2766)
                     /label=SMP4960
     primer_bind     2789..2805
                     /label=TET-CMV-for
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2827..2829,2830..3150)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     primer_bind     complement(3083..3094)
                     /label=SMP296
     primer_bind     3107..3126
                     /label=SMP3313
     CDS             3157..3477
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             join(3496..3498,3499..4209)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     3496..3516
                     /label=SMP4637
     primer_bind     3496..3513
                     /label=SMP2824
     primer_bind     complement(3496..3513)
                     /label=SMP3729
     primer_bind     3499..3516
                     /label=SMP2016
     primer_bind     complement(3535..3551)
                     /label=PEN1-737R
     primer_bind     complement(3538..3559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3540..3560)
                     /label=SMP919
     primer_bind     complement(3555..3573)
                     /label=SMP2564
     primer_bind     complement(3566..3586)
                     /label=SMP2562
     primer_bind     complement(3568..3586)
                     /label=EGFP-N-R+33
     primer_bind     complement(3601..3619)
                     /label=EGFP-N-rev
     primer_bind     complement(3609..3628)
                     /label=SMP2433
     primer_bind     complement(3624..3642)
                     /label=SMP3388
     primer_bind     3667..3687
                     /label=SMP2316
     primer_bind     3741..3760
                     /label=SMP1001
     primer_bind     3787..3806
                     /label=SMP1325
     primer_bind     complement(3797..3817)
                     /label=SMP2969
     primer_bind     complement(3799..3818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(3854..3874)
                     /label=SMP2971
     primer_bind     complement(3857..3873)
                     /label=SMP2578
     primer_bind     complement(3887..3900)
                     /label=SMP2969
     primer_bind     3900..3918
                     /label=SMP444
     primer_bind     3910..3934
                     /label=SMP1666
     primer_bind     3964..3981
                     /label=SMP3040
     primer_bind     3964..3981
                     /label=SMP3104
     primer_bind     3969..3988
                     /label=SMP1330
     primer_bind     3995..4012
                     /label=SMP4080
     primer_bind     3996..4015
                     /label=SMP437
     primer_bind     complement(3996..4015)
                     /label=SMP471
     primer_bind     4034..4051
                     /label=SMP3892
     primer_bind     4047..4066
                     /label=SMP364
     primer_bind     complement(4078..4121)
                     /label=SMP4989
     primer_bind     4096..4128
                     /label=SMP295
     primer_bind     complement(4096..4128)
                     /label=SMP296
     primer_bind     4105..4146
                     /label=SMP4988
     primer_bind     4119..4137
                     /label=EGFP-C-F-31
     primer_bind     4128..4144
                     /label=EGFP-for
     primer_bind     4134..4152
                     /label=SMP564
     primer_bind     4146..4167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4150..4167
                     /label=EGFP-C-for
     primer_bind     4182..4219
                     /label=SMP2241
     primer_bind     complement(4182..4200)
                     /label=SMP2415
     primer_bind     complement(4189..4209)
                     /label=SMP2429
     primer_bind     4189..4209
                     /label=SMP3728
     primer_bind     complement(4189..4208)
                     /label=SMP2596
     primer_bind     complement(4191..4219)
                     /label=SMP4373
     primer_bind     complement(4207..4233)
                     /label=SMP884
     primer_bind     4210..4229
                     /label=SMP4977
     primer_bind     complement(4213..4233)
                     /label=SMP1201
     primer_bind     4215..4278
                     /label=SMP5000
     primer_bind     4216..4237
                     /label=SMP4205
     primer_bind     complement(4225..4244)
                     /label=SMP4877
     primer_bind     complement(4231..4254)
                     /label=SMP1053
     misc_feature    4235..4278
                     /label=primer fw
     misc_feature    4255..6099
                     /label=SINTBAD
     primer_bind     4844..4862
                     /label=SMP3466
     misc_feature    5201..5223
                     /label=seq
     misc_feature    6078..6122
                     /label=primer rev
     primer_bind     complement(6078..6108)
                     /label=SMP5001
     misc_feature    6110..6698
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6163..6183)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(6165..6188)
                     /label=SMP3898
     primer_bind     complement(6166..6186)
                     /label=SMP1896
     primer_bind     complement(6177..6196)
                     /label=WHV-5R
     primer_bind     complement(6533..6569)
                     /label=SMP4902
     primer_bind     6543..6581
                     /label=SMP4901
     primer_bind     6543..6569
                     /label=SMP4740
     primer_bind     complement(6543..6569)
                     /label=SMP4741
     CDS             complement(6581..6592)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6773..7006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7118..7136)
                     /label=QE-for
     primer_bind     complement(7125..7143)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     7210..7230
                     /label=SMP646
     primer_bind     complement(7210..7230)
                     /label=SMP654
     promoter        7211..7315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7316..7384,7385..8176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7361..7380)
                     /label=AmpStart
     primer_bind     complement(7534..7553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     8107..8126
                     /label=AmpStop
     primer_bind     complement(8151..8184)
                     /label=SMP4838
     primer_bind     8160..8199
                     /label=SMP4839
     rep_origin      8347..8935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8727..8744
                     /label=SMP4077
     primer_bind     8836..8855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9089..9106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9198..9217
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9221..9232)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9314..9331
                     /label=SMP2212
     primer_bind     complement(9316..9333)
                     /label=SMP4078
     protein_bind    9346..9367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9382..9387,9388..9405,9406..9412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     9393..9412
                     /label=pUCM13-rev-157
     protein_bind    9420..9436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9425..9447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9427..9447
                     /label=SMP547
     primer_bind     9427..9446
                     /label=LacOp-for
     primer_bind     9439..9458
                     /label=SMP993
     primer_bind     9444..9460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9444..9460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9497..9518)
                     /label=SMP3893
     primer_bind     9510..9529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcggtacc
     2821 gccaccatgg gagtgcaggt ggaaaccatc tccccaggag acgggcgcac cttccccaag
     2881 cgcggccaga cctgcgtggt gcactacacc gggatgcttg aagatggaaa gaaatttgat
     2941 tcctcccggg acagaaacaa gccctttaag tttatgctag gcaagcagga ggtgatccga
     3001 ggctgggaag aaggggttgc ccagatgagt gtgggtcaga gagccaaact gactatatct
     3061 ccagattatg cctatggtgc cactgggcac ccaggcatca tcccaccaca tgccactctc
     3121 gtcttcgatg tggagcttct aaaactggaa actagaggcg tgcaggtcga gaccatcagc
     3181 cccggcgacg gccgcacctt tcccaagaga ggccagactt gcgtggtcca ctacaccggc
     3241 atgctggagg acggcaagaa gttcgacagc agccgcgacc gcaacaagcc cttcaagttc
     3301 atgctgggca aacaggaagt gatccgcggc tgggaggaag gcgtggctca gatgagcgtg
     3361 gggcagcggg ccaagctgac catcagcccc gactatgcct acggcgccac cggccacccc
     3421 ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg agctgctgaa gctggagact
     3481 agtaccggtt ctagagtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3541 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3601 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3661 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     3721 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3781 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3841 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3901 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     3961 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4021 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4081 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     4141 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4201 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacgagtcc
     4261 atgttcgagg acgacatcag catcctgacg caggaggccc tggggcctag tgaggtgtgg
     4321 ctggacagtc ccggagaccc ctcgcttggg ggcgacatgt gctccgcctc ccactttgcc
     4381 ctcatcactg cttacggaga catcaaggaa cggctggggg gcctggagag ggagaacgcc
     4441 accctccgac gccgcctcaa agtctacgag atcaagtacc cactgatcag tgactttgga
     4501 gaggagcatg gcttttctct gtatgaaatc aaggatggct ccctgctgga ggtggagaag
     4561 gtcagcctgc agcaacggct caaccagttc cagcatgagt tacagaagaa caaggagcag
     4621 gaagaacagc ttggagagat gatccaggcc tacgagaaac tctgcgtgga gaagagtgac
     4681 ttggagacag agctgaggga gatgagggcc ctggtggaga cgcacctgcg tcagatctgt
     4741 ggtttggagc agcagctgcg gcaacagcaa ggcctccagg atgcagcctt ctccaacctg
     4801 agcccaccgc cagcccccgc ccctccctgc actgatttag acctgcacta cctggcactg
     4861 agagggggat ctggcttgag tcatgcaggc tggccgggct ccacacccag tgtgagtgac
     4921 ctggagcggc ggcggctaga agaggctttg gaggccgcgc agggagaggc ccggggggct
     4981 cagctccggg aggagcagct ccaggccgag tgcgagcggc tgcaggggga gctgaagcag
     5041 ctgcaggaga cccgggccca ggatctggcc tccaaccagt cggagcgaga catggcgtgg
     5101 gtgaaaagag ttggggatga tcaggtgaat ttggcgctgg cctacaccga gctgacggag
     5161 gagctgggcc ggcttcggga gttgagttcc ctacagggga gaatcttgag gactctgttg
     5221 caggaacagg cccggagtgg cggccagagg cactcgccgc tgtcacaacg ccactccccg
     5281 gccccccagt gcccctcccc ctccccgcct gcccgagcgg ctcccccgtg ccccccgtgc
     5341 cagtcccccg tcccccagcg ccgctctccc gtgcccccgt gcccctcgcc gcagcagcgc
     5401 cgctctccgg cctcaccctc ctgcccgtcg cccgtcccgc agcgccgctc gccggtgccg
     5461 ccgtcgtgcc agtcccccag cccgcagcgc cgttccccgg tgccccccag ctgcccggcc
     5521 ccgcagcccc ggccaccgcc gccgcccccg ccgggcgaga ggacgctggc cgagcgcgcc
     5581 tacgccaagc cgcccagcca ccacgtgaag gccggcttcc agggccgccg cagctactct
     5641 gagctggcgg agggcgcggc ctacgcgggc gcctccccgc cctggctgca ggccgaagcg
     5701 gccactctcc ccaagccccg ggcctacggc agcgagctct acggccctgg caggcccctc
     5761 agcccgcggc gcgccttcga gggcatccgg ctgcgcttcg agaagcagcc gtcggaggag
     5821 gacgagtggg ctgtgcccac cagcccgccc agcccggagg tgggcaccat ccgctgcgcc
     5881 tccttctgcg cgggcttccc catccccgag tcgcccgccg ccaccgccta cgcccacgcc
     5941 gagcacgcgc agtcctggcc gtccatcaac ttgctgatgg agacggtggg ctccgacatc
     6001 cgcagctgcc ccctctgcca gctgggtttc cctgtcgggt acccggatga tgccctcatc
     6061 aaacacattg actcccacct ggagaacagc aagatcggat agggatccta atcaacctct
     6121 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6181 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6241 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6301 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6361 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6421 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6481 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6541 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6601 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6661 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
     6721 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     6781 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     6841 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6901 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6961 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     7021 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
     7081 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
     7141 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     7201 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7261 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7321 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7381 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7441 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7501 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     7561 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7621 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7681 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7741 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7801 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7861 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7921 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7981 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8041 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8101 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8161 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8221 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8281 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8341 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8401 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8461 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8521 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8581 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8641 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8701 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8761 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8821 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8881 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8941 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9001 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9061 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9121 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
     9181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
     9301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     9361 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     9421 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
     9481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     9541 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     9601 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     9661 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
