LOCUS       SMT000                  2741 bp ds-DNA     circular     25-JUL-2022
DEFINITION  .
COMMENT     This plasmid is an exact copy of the pSB1C3-pT7_sfGFP plasmid from
            Akshay. Chloramphenicol resistance. Original Sequencing Files
FEATURES             Location/Qualifiers
     primer          1..29
                     /label="SMT000_bead_amp_1000_fwd"
                     /note="sequence: tggtcatgagattatcaaaaaggatcttc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    113..138
                     /label="CamR REV primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     stem_loop       113..218
                     /label="T0 terminator"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     cds             complement(231..890)
                     /label="Chloramphenicol resistance"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          524..551
                     /label="SMT000_bead_amp_500_fwd"
                     /note="sequence: cttgcgaatatatgtgtagaaactgccg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          774..796
                     /label="SMT000_bead_amp_250_fwd"
                     /note="sequence: cagctgaacggtctggttatagg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          874..905
                     /label="SMT000_bead_amp_150_fwd"
                     /note="sequence: gtgatttttttctccattttagcttccttagc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(891..996)
                     /label="Unnanotated Promoter (P1004)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          975..996
                     /label="SMT000_bead_amp_50_fwd"
                     /note="sequence: acctcttacgtgcccgatcaac"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          975..996
                     /label="SMT000_IVTT_fwd"
                     /note="sequence: ACCTCTTACGTGCCCGATCAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          975..996
                     /label="SMT000 NAQ_seq1"
                     /note="sequence: ACCTCTTACGTGCCCGATCAAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(975..996)
                     /label="CamR FOR primer"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1003..1024)
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_binding  1004..1023
                     /label="Verification forward (VF2) primer binding site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1028..1044
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1036..1060
                     /label="SMT000 Gib Ins Rnd2 Fwd"
                     /note="sequence: ctcactatagggcttaagtataagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1036..1073)
                     /label="SMT000 Gib Vec Rev"
                     /note="sequence: catatgtataTCTCCTTatacttaagccctatagtgag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1057..1063
                     /label="RBS?"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1064..1070
                     /label="FC013A Spacer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1071..1787
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1071..1787
                     /label="Translation 1071-1787"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYK*"
     misc_feature    complement(1072..1101)
                     /label="GFP BE NT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1209..1238
                     /label="GFP ME T"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1239..1268)
                     /label="GFP ME NT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1721..1750)
                     /label="GFP FE NT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1733..1762
                     /label="GFP FE T"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     barcode         1788..1812
                     /label="Help:Barcodes"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1788..1812
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     dna             1813..1833
                     /label="BioBrick suffix"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1823..1839
                     /label="SMT000 Gib Vec fwd"
                     /note="sequence: ggccgctgcagtccggc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     stem_loop       1834..1905
                     /label="E. coli his operon terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     stem_loop       1842..1876
                     /label="Stem loop"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1847..1862)
                     /label="SMT000 Gibs Ins Rnd2 Rev"
                     /note="sequence: gtggtgacaccttgcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1890..1914)
                     /label="pSB1C3 anneal REV"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(1940..1955)
                     /label="SMT000_bead_amp_50_rev"
                     /note="sequence: gccgcagccgaacgac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1968..1991)
                     /label="SMT000_IVTT_rev"
                     /note="sequence: ACCGTATTACCGCCTTTGAGTGAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_binding  complement(1969..1988)
                     /label="Verification reverse (VR) primer binding site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2038..2055)
                     /label="SMT000_bead_amp_150_rev"
                     /note="sequence: ctggccttttgctggcct"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc            complement(2073..2687)
                     /label="rep (pMB1)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc            2088..2088
                     /label="ORI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(2138..2155)
                     /label="SMT000_bead_amp_250_rev"
                     /note="sequence: cgggtttcgccacctctg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2380..2405)
                     /label="SMT000_bead_amp_500_rev"
                     /note="sequence: ggttggactcaagacgatagttaccg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(2715..2741)
                     /label="SMT000_bead_amp_1000_rev"
                     /note="sequence: aaatcccttaacgtgagttttcgttcc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
       61 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct
      121 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      181 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      241 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      301 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      361 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      421 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
      481 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
      541 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
      601 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
      661 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
      721 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
      781 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
      841 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
      901 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
      961 tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtGCCACCTG ACGTCTAAGA
     1021 AACCgaataa tacgactcac tatagggctt aagtatAAGG AGAtatacat ATGAGCAAAG
     1081 GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA ATTAGATGGT GATGTTAATG
     1141 GGCACAAATT TTCTGTCCGT GGAGAGGGTG AAGGTGATGC TACAAACGGA AAACTCACCC
     1201 TTAAATTTAT TTGCACTACT GGAAAACTAC CTGTTCCGTG GCCAACACTT GTCACTACTC
     1261 TGACCTATGG TGTTCAATGC TTTTCCCGTT ATCCGGATCA CATGAAACGG CATGACTTTT
     1321 TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAACGCAC TATATCTTTC AAAGATGACG
     1381 GGACCTACAA GACGCGTGCT GAAGTCAAGT TTGAAGGTGA TACCCTTGTT AATCGTATCG
     1441 AGTTAAAGGG TATTGATTTT AAAGAAGATG GAAACATTCT TGGACACAAA CTCGAGTACA
     1501 ACTTTAACTC ACACAATGTA TACATCACGG CAGACAAACA AAAGAATGGA ATCAAAGCTA
     1561 ACTTCAAAAT TCGCCACAAC GTTGAAGATG GTTCCGTTCA ACTAGCAGAC CATTATCAAC
     1621 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC CTGTCGACAC
     1681 AATCTGTCCT TTCGAAAGAT CCCAACGAAA AGCGTGACCA CATGGTCCTT CTTGAGTTTG
     1741 TAACTGCTGC TGGGATTACA CATGGCATGG ATGAGCTCTA CAAAtaaCGC TGATAGTGCT
     1801 AGTGTAGATC gctactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
     1861 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     1921 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1981 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2041 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     2101 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2161 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2221 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2281 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2341 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2401 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2461 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2521 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2581 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2641 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2701 gtctgacgct cagtggaacg aaaactcacg ttaagggatt t
//