LOCUS       pCLX-KRT7-GFP           8824 bp    DNA     circular     23-AUG-2010
DEFINITION  attB1-attB2-attB4.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|555342280|
COMMENT     VNTDBDATE|563280008|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCLX-KRT7-GFP|
COMMENT     VNTAUTHORNAME|Patrick Salmon|
COMMENT     VNTAUTHORTEL|+41223795670|
COMMENT     VNTAUTHORFAX|+41223795452|
COMMENT     VNTAUTHOREML|patrick.salmon@unige.ch|
COMMENT     VNTAUTHORWWW|http://medweb2.unige.ch/salmon/|
COMMENT     VNTAUTHORAD1|Department of Basic Neuroscience|
COMMENT     VNTAUTHORAD2|Faculty of Medecine (CMU)|
COMMENT     VNTAUTHORAD3|1 rue Michel Servet|
COMMENT     VNTAUTHORAD4|CH-1211 Geneva 4 - Switzerland|
COMMENT     VNTUDF|Tested?|5|Yes, works|
FEATURES             Location/Qualifiers
     misc_feature    4050..4070
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(4807..4827)
                     /vntifkey="21"
                     /label=attB2
     misc_feature    2522..2542
                     /vntifkey="21"
                     /label=attB4
     misc_feature    4842..5439
                     /vntifkey="21"
                     /label=WPRE
     misc_marker     5458..5521
                     /vntifkey="22"
                     /label=Nef*
                     /note="5' end of Nef ORF"
     LTR             5522..5556
                     /vntifkey="19"
                     /label=U3
                     /note="U3 SIN of HIV LTR"
     misc_recomb     5557..5611
                     /vntifkey="86"
                     /label=LoxP
                     /note="LoxP sequence target of bacterial Cre recombinase"
     LTR             5612..5630
                     /vntifkey="19"
                     /label=U3
                     /note="U3 SIN LTR of HIV"
     repeat_unit     5631..5727
                     /vntifkey="35"
                     /label=R
                     /note="R of LTR of HIV"
     LTR             5728..5810
                     /vntifkey="19"
                     /label=U5
                     /note="U5 of LTR of HIV"
     splicing_signal 2386..2388
                     /vntifkey="38"
                     /label=SA
                     /note="HIV splice acceptor"
     promoter        1..384
                     /gene="CMV/e"
                     /vntifkey="29"
                     /label=CMVie
     LTR             383..642
                     /gene="LTR"
                     /vntifkey="19"
                     /label=RU5
     protein_bind    798..1157
                     /gene="gag*"
                     /vntifkey="31"
                     /label=psi(dGAG)
     misc_structure  1174..1621
                     /gene="''pol"
                     /vntifkey="88"
                     /label=cPPT-CTS
     polyA_signal    5846..6297
                     /gene="rBGpA"
                     /vntifkey="25"
                     /label=rBGpA
                     /note="rabbit beta-globin polyA"
     rep_origin      6495..6831
                     /gene="SV40 ori"
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             complement(7833..8693)
                     /vntifkey="4"
                     /label=Amp
     TATA_signal     3986..3992
                     /vntifkey="41"
                     /label=TATA\box
     promoter        2543..4049
                     /vntifkey="29"
                     /label=Krt7\promoter
                     /note="mouse keratin 7 promoter"
     CDS             4083..4799
                     /vntifkey="4"
                     /label=EGFP
BASE COUNT     2314 a      2112 c      2287 g      2111 t 
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
      361 tattagtcat cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc 
      421 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc 
      481 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
      541 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
      601 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga 
      661 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc 
      721 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 
      781 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 
      841 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 
      901 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 
      961 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 
     1021 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 
     1081 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa 
     1141 agcacagcaa gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg 
     1201 agtagtagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc 
     1261 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg 
     1321 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 
     1381 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 
     1441 cagcagaaat ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt 
     1501 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag 
     1561 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta 
     1621 gaacatggaa aagtttagta aaacaccata aggaggagat atgagggaca attggagaag 
     1681 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1741 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1801 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1861 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1921 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1981 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     2041 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     2101 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     2161 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     2221 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     2281 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     2341 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     2401 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     2461 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgctagt gattatcgga 
     2521 tcaactttgt atagaaaagt tggctagcac tggagtttta aagaatgttc cagggagggg 
     2581 taagaggccc agggcagatg tggggtaggc tcaggaatga ggaagtattt ctcaagcctg 
     2641 tgtgtggtgg ttataggggg ccgaggcctg gactgaagtg cccaggtact tttgtctgtg 
     2701 tgcttcccag ctcttgttag accctcatcc tgaaggagct gtggctgagg gggccaggac 
     2761 ccgtccaggt cagcgcacta gctgtgccct gctggggctc agcttgagaa agagaaggag 
     2821 ctcttggtgt gggtgagaaa caacctccaa agtgggggat gggtggcagg ggctgtagca 
     2881 ggatggaaag tggggtgcag agggggacaa aggactgagg aagcaccttg aggggtaggg 
     2941 agtctccttg agagcttagg gttgtctacc tgaaaaggca aagcaggaag aatatgacat 
     3001 tcccatcctc cccacctagg acgcccctaa aggaccagtc tcactgcatg gagccagagg 
     3061 tgggctaaag gctgtccaag tgagtctggg aaaactggaa gtccaggagc actccgagga 
     3121 gcactgagca caccaccacg ggttaccaca gaggccagtt ttagttgatt cccttgtcag 
     3181 gcatagccta gtgggtttgt aagcaggaaa aatgaggcag agcagggagg aacgtctgga 
     3241 tgtgaacggg agacaggaca cacacatgtg catacaaatg tgttcagttg tgaatccaga 
     3301 ccccccatgc acagactgca ctcatataca ctcacactag tgtgttaggg gtatgagggc 
     3361 acagaagggt acgcacagcc atcccaggaa cccatacagt agtttgagga gggggtgagg 
     3421 caagccctct gaacttggat ggtggggagg gcgcagttac tgaaacagct cttcccagta 
     3481 aggatcgtca cagggccagg ttactcaaga actgagctga gctctccagt agcagctggg 
     3541 ccaggaagga gagtcctagg aagcctaggg cttccccagc cagtgggtga ggggtttcaa 
     3601 ctttcagcag gagctaaagt ttgtcaggca cttccttttg tagcacttgg ctttcctact 
     3661 ggcctcagga atctaggtat tcactcctca tacctgctct aggaagcctt ctcctgccaa 
     3721 ttgtaaggtg gtctctttgc ggtccagaga tttctgggct gtgggtatct tggacaactt 
     3781 gcctgaggtg tgggtgtctc acgacggcgt ctcggcctcc tgcagacact tcccgagtgg 
     3841 acgaagagcc ttgggcggcg gctgggggcg gtgcgagggc agaaggcagg cggacaggaa 
     3901 ggccctggct gcagagaaag gtggccagcg gccagccccg ccctgtcccc acctgtgtgc 
     3961 gctcagcctc cgccccgcat ggaggaataa aaggtcgcag actgcctgag gtcagggagt 
     4021 tcgcgctgct cctcgcctcg tccagccagc aactttgtac aaaaaagcag gctcccgcca 
     4081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 
     4141 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 
     4201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 
     4261 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 
     4321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 
     4381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 
     4441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 
     4501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 
     4561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 
     4621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 
     4681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 
     4741 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 
     4801 aagacccagc tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat 
     4861 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 
     4921 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 
     4981 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 
     5041 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 
     5101 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 
     5161 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 
     5221 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc 
     5281 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 
     5341 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag 
     5401 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgcttta 
     5461 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 
     5521 ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc atgggaataa 
     5581 cttcgtatag catacattat acgaagttat gctgcttttt gcttgtactg ggtctctctg 
     5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
     5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
     5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagtt 
     5821 gaatcgccag tgtgtcgagc tagcagatct ttttccctct gccaaaaatt atggggacat 
     5881 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat 
     5941 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt 
     6001 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgccatat gctggctgcc 
     6061 atgaacaaag gtggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 
     6121 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 
     6181 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 
     6241 tcctctcctg actactccca gtcatagctg tccctcttct cttatgaaga tccctcgacc 
     6301 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     6361 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     6421 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     6481 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc 
     6541 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     6601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     6661 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta 
     6721 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     6781 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg 
     6841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     6901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     6961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     7021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     7081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     7141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     7201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     7261 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     7321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     7381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     7441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     7501 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     7561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     7621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     7681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     7741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     7801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     7861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     7921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 
     7981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 
     8041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 
     8101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 
     8161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 
     8221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 
     8281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 
     8341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 
     8401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 
     8461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 
     8521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 
     8581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 
     8641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 
     8701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 
     8761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 
     8821 cctg 
//
