LOCUS       pc4632-pEGFP-hTipin     6126 bp ds-DNA     circular     23-JAN-2024
DEFINITION  .
COMMENT     Ligation of : pc624-pENeGFPRPA34.xdna [5208  nt] : (XbaI[2298] /
            HindIII[1467]) to hTipinSequencePCR_for_cloning.xdna [910  nt] :
            (HindIII[7] / XbaI[921])
FEATURES             Location/Qualifiers
     promoter        17..543
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             731..1444
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1467..2381
                     /label="hTipin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(3635..3712)
                     /label="SV40 ORI"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(3974..4602)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             complement(4754..5413)
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Promoter        complement(5653..5681)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 GAATTCGAGC TCGCCCCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA
       61 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA
      121 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC
      181 AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT
      241 GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT
      301 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC
      361 GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT
      421 GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA
      481 TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC
      541 AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT
      601 CCAGCCTCCG CGGGATCTTG GTGGCGTGAA ACTCCCGCAC CTCTTCGGCC AGCGCCTTGT
      661 AGAAGCGCGT ATGGCTTCGT GGGGATCCCC GAAGAAGAAG CGCAAAGTAC TGGTACCGGT
      721 CGCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
      781 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
      841 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
      901 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
      961 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
     1021 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
     1081 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
     1141 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
     1201 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
     1261 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
     1321 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
     1381 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
     1441 CAAGTCCGGA CTCAGATCTC GAGCTCAagc tTCGATGCTA GAACCACAGG AGAATGGCGT
     1501 GATTGACCTA CCAGATTATG AGCATGTAGA AGATGAAACT TTTCCTCCTT TCCCACCTCC
     1561 AGCCTCTCCA GAGAGACAAG ATGGTGAAGG AACTGAGCCT GATGAAGAGT CAGGAAATGG
     1621 AGCACCTGTT CCTGTACCTC CAAAGAGAAC AGTTAAAAGA AATATACCCA AGCTGGATGC
     1681 TCAGAGATTA ATTTCAGAGA GAGGACTTCC AGCCTTAAGG CATGTATTTG ATAAGGCAAA
     1741 ATTCAAAGGT AAAGGTCATG AGGCTGAAGA CTTGAAGATG CTAATCAGAC ACATGGAGCA
     1801 CTGGGCACAT AGGCTATTCC CTAAACTGCA GTTTGAGGAT TTTATTGACA GAGTTGAATA
     1861 CCTGGGAAGT AAAAAGGAAG TTCAGACCTG TTTAAAACGA ATTCGACTTG ATCTCCCTAT
     1921 TTTACATGAA GATTTTGTTA GCAATAATGA TGAAGTTGCG GAGAATAATG AACATGATGT
     1981 CACTTCTACT GAATTAGATC CCTTTCTGAC AAACTTATCT GAAAGTGAGA TGTTTGCTTC
     2041 TGAGTTAAGT AGAAGCCTAA CAGAAGAGCA ACAACAAAGA ATTGAGAGAA ATAAACAACT
     2101 GGCCTTGGAA nGAAGGCAGG CAAAGCTGCT GAGTAATAGT CAGACCCTAG GAAATGATAT
     2161 GTTAATGAAT ACACCcAGGg CACACACGGT TGAAGAGGTT AATACTGATG AGGATCAAAA
     2221 GGAGGAGTCA AATGGATTAA ACGAAGACAT TCTGGACAAT CCATGTAATG ATGCTATTGC
     2281 CAATACTTTA AATGAAGAGG AAACACTGCT GGACCAGTCT TTTAAAAAtG TGCAACAGCA
     2341 ACTTGATGCT ACATCCAGAA ATATTACTGA AGCTAGATGA TctagAGTCG AGATCCTGAG
     2401 AACTTCAGGG TGAGTTTGGG GACCCTTGAT TGTTCTTTCT TTTTCGCTAT TGTAAAATTC
     2461 ATGTTATATG GAGGGGGCAA AGTTTTCAGG GTGTTGTTTA GAATGGGAAG ATGTCCCTTG
     2521 TATCACCATG GACCCTCATG ATAATTTTGT TTCTTTCACT TTCTACTCTG TTGACAACCA
     2581 TTGTCTCCTC TTATTTTCTT TTCATTTTCT GTAACTTTTT CGTTAAACTT TAGCTTGCAT
     2641 TTGTAACGAA TTTTTAAATT CACTTTTGTT TATTTGTCAG ATTGTAAGTA CTTTCTCTAA
     2701 TCACTTTTTT TTCAAGGCAA TCAGGGTATA TTATATTGTA CTTCAGCACA GTTTTAGAGA
     2761 ACAATTGTTA TAATTAAATG ATAAGGTAGA ATATTTCTGC ATATAAATTC TGGCTGGCGT
     2821 GGAAATATTC TTATTGGTAG AAACAACTAC ATCCTGGTCA TCATCCTGCC TTTCTCTTTA
     2881 TGGTTACAAT GATATACACT GTTTGAGATG AGGATAAAAT ACTCTGAGTC CAAACCGGGC
     2941 CCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG
     3001 GTTGTTGTGC TGTCTCATCA TTTTGGCAAA GAATTCACTC CTCAGGTGCA GGCTGCCTAT
     3061 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT
     3121 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
     3181 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
     3241 AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG
     3301 GCAACATATG CCATATGCTG GCTGCCATGA ACAAAGGTGG CTATAAAGAG GTCATCAGTA
     3361 TATGAAACAG CCCCCTGCTG TCCATTCCTT ATTCCATAGA AAAGCCTTGA CTTGAGGTTA
     3421 GATTTTTTTT ATATTTTGTT TTGTGTTATT TTTTTCTTTA ACATCCCTAA AATTTTCCTT
     3481 ACATGTTTTA CTAGCCAGAT TTTTCCTCCT CTCCTGACTA CTCCCAGTCA TAGCTGTCCC
     3541 TCTTCTCTTA TGAACGGAGC TTTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT
     3601 ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA
     3661 GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG
     3721 TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGACTGCA TTAATGAATC GGCCAACGCG
     3781 CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
     3841 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     3901 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     3961 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     4021 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     4081 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     4141 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     4201 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     4261 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     4321 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     4381 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
     4441 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     4501 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     4561 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     4621 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
     4681 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
     4741 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
     4801 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
     4861 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
     4921 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
     4981 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
     5041 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
     5101 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
     5161 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
     5221 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
     5281 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
     5341 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
     5401 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
     5461 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
     5521 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
     5581 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
     5641 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
     5701 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA
     5761 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT
     5821 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
     5881 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
     5941 GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATCG
     6001 ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT
     6061 GAGCACCGCC GCCGCAAGGA ATGGTGCTGG CTTATCGAAA TTAATCGACT CACTATAGGG
     6121 AGACCC
//