LOCUS       pLV-TRE-Stat3-2A-BFP    9629 bp ds-DNA     circular     23-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          1..20
                     /label="pLV-TRE FWD"
                     /note="sequence: cctttgggccgcctccccgcgcgggactctgggtaccttt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     LTR             83..316
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: self-inactivating 3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    388..509
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     rep_origin      549..684
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     promoter        complement(705..723)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(733..749)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     rep_origin      891..1346
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        1372..1476
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     rep_origin      2508..3096
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     protein_bind    3384..3405
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        3420..3425
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        3426..3443
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        3444..3450
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    3458..3474
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     3482..3498
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     promoter        3519..3537
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     promoter        3565..3791
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: Rous sarcoma virus enhancer/promoter"
     LTR             3792..3972
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4019..4144
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: packaging signal of human immunodeficiency virus type 1"
     misc_feature    4637..4870
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     misc_feature    5365..5482
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: central polypurine tract and central termination sequence of HIV-1"
     protein_bind    5500..5520
                     /label="attB4"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: BP Clonase™ gene: mutant version of attB note: core recombination site for the Gateway® BP reaction"
     protein_bind    5545..5563
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5564..5586
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5587..5605
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5606..5628
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5629..5647
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5648..5670
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5671..5689
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5690..5712
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5713..5731
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5732..5754
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5755..5773
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5774..5796
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     protein_bind    5797..5815
                     /label="tetracycline response element"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR note: contains seven copies of the tetracycline operator tetO"
     primer          5858..5874
                     /label="pLV TRE UP"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cgtcagatcgcctggag"
     protein_bind    5944..5968
                     /label="attB1"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: BP Clonase™ gene: mutant version of attB note: recombination site for the Gateway® BP reaction"
     primer          complement(5955..5974)
                     /label="pLV-TRE REV"
                     /note="sequence: AGCTGGTTCCACTGAGCCATggtggcagcctgcttttttg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5969..5974
                     /label="KOZAK"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    5969..5978
                     /label="kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5975..5994
                     /label="Stat3 gene FWD"
                     /note="sequence: caaaaaagcaggctgccaccATGGCTCAGTGGAACCAGCT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     exon            5975..6102
                     /label="Stat3-203 Exon 2"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     gene            5975..8284
                     /label="Stat3"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             5975..9040
                     /label="protein"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPMEGRGSLLTCGDVEENPGPMSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
     exon            6103..6247
                     /label="Stat3-203 Exon 3"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            6248..6346
                     /label="Stat3-203 Exon 4"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            6347..6442
                     /label="Stat3-203 Exon 5"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     exon            6443..6524
                     /label="Stat3-203 Exon 6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     exon            6525..6619
                     /label="Stat3-203 Exon 7"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     exon            6620..6771
                     /label="Stat3-203 Exon 8"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            6772..6930
                     /label="Stat3-203 Exon 9"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            6931..7023
                     /label="Stat3-203 Exon 10"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     exon            7024..7083
                     /label="Stat3-203 Exon 11"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     exon            7084..7113
                     /label="Stat3-203 Exon 12"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            7114..7207
                     /label="Stat3-203 Exon 13"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            7208..7255
                     /label="Stat3-203 Exon 14"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     exon            7256..7339
                     /label="Stat3-203 Exon 15"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     exon            7340..7438
                     /label="Stat3-203 Exon 16"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            7439..7574
                     /label="Stat3-203 Exon 17"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     exon            7575..7627
                     /label="Stat3-203 Exon 18"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     exon            7628..7722
                     /label="Stat3-203 Exon 19"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     exon            7723..7862
                     /label="Stat3-203 Exon 20"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     exon            7863..8075
                     /label="Stat3-203 Exon 21"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     exon            8076..8118
                     /label="Stat3-203 Exon 22"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     exon            8119..8231
                     /label="Stat3-203 Exon 23"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     exon            8232..8284
                     /label="Stat3-203 Exon 24"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(8265..8284)
                     /label="Stat3 gene REV"
                     /note="sequence: aggagacttcctctgccctcCATGGGGGAGGTAGCACACT"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          8285..8304
                     /label="T2ABFP FWD"
                     /note="sequence: AGTGTGCTACCTCCCCCATGgagggcagaggaagtctcct"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             8285..8338
                     /label="T2A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     source          8285..9040
                     /label="source"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             8339..9040
                     /label="TagBFP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(9021..9040)
                     /label="T2ABFP REV"
                     /note="sequence: ttgtaatccagaggttgatttcaattaagcttgtgcccca"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    9041..9629
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
     primer          9042..9081
                     /label="WPRE-from-Aass-FWD"
                     /note="sequence: tggggcacaagcttaattgaaatcaacctctggattacaaaatttgtgaaagattgactg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(9109..9126)
                     /label="pLV TRE Dwn"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gcagcgtatccacatagc"
     primer          complement(9610..9629)
                     /label="WPRE-from-Aass-REV"
                     /note="sequence: aaaggtacccagagtcccgcgcggggaggcggcccaaagg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 gcgggactct gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
       61 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
      121 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
      181 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      241 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      301 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
      361 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
      421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      481 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
      541 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      601 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      661 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
      721 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
      781 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
      841 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
      901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      961 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1021 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1081 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1141 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1201 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1261 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1321 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1381 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1441 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1501 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1561 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1621 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1681 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1741 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1801 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1861 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1921 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     1981 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2041 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2101 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2161 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2221 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2281 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2341 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2401 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2461 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2521 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2581 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2641 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2701 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2761 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2821 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2881 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2941 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3001 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3061 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3121 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3181 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3241 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3301 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3361 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     3421 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     3481 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     3541 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     3601 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     3661 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     3721 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     3781 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3841 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3901 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3961 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     4021 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     4081 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     4141 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     4201 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     4261 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     4321 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     4381 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     4441 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     4501 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     4561 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     4621 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     4681 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     4741 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     4801 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4861 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4921 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4981 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     5041 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     5101 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     5161 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     5221 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     5281 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     5341 acggatctcg acggtatcgc tagcttttaa aagaaaaggg gggattgggg ggtacagtgc
     5401 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5461 aattacaaaa attcaaaatt ttactagtga ttatcggatc aactttgtat agaaaagttg
     5521 ggtaccgagc tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
     5581 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
     5641 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
     5701 gactttcact tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta
     5761 tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat agggagtggt
     5821 aaactcgacc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     5881 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
     5941 attgcaagtt tgtacaaaaa agcaggctgc caccATGGCT CAGTGGAACC AGCTGCAGCA
     6001 GCTGGACACA CGCTACCTGG AGCAGCTGCA CCAGCTGTAC AGCGACAGCT TCCCCATGGA
     6061 GCTGCGGCAG TTCCTGGCAC CTTGGATTGA GAGTCAAGAC TGGGCATATG CAGCCAGCAA
     6121 AGAGTCACAT GCCACGTTGG TGTTTCATAA TCTCTTGGGT GAAATTGACC AGCAATATAG
     6181 CCGATTCCTG CAAGAGTCCA ATGTCCTCTA TCAGCACAAC CTTCGAAGAA TCAAGCAGTT
     6241 TCTGCAGAGC AGGTATCTTG AGAAGCCAAT GGAAATTGCC CGGATCGTGG CCCGATGCCT
     6301 GTGGGAAGAG TCTCGCCTCC TCCAGACGGC AGCCACGGCA GCCCAGCAAG GGGGCCAGGC
     6361 CAACCACCCA ACAGCCGCCG TAGTGACAGA GAAGCAGCAG ATGTTGGAGC AGCATCTTCA
     6421 GGATGTCCGG AAGCGAGTGC AGGATCTAGA ACAGAAAATG AAGGTGGTGG AGAACCTCCA
     6481 GGACGACTTT GATTTCAACT ACAAAACCCT CAAGAGCCAA GGAGACATGC AGGATCTGAA
     6541 TGGAAACAAC CAGTCTGTGA CCAGACAGAA GATGCAGCAG CTGGAACAGA TGCTCACAGC
     6601 CCTGGACCAG ATGCGGAGAA GCATTGTGAG TGAGCTGGCG GGGCTCTTGT CAGCAATGGA
     6661 GTACGTGCAG AAGACACTGA CTGATGAAGA GCTGGCTGAC TGGAAGAGGC GGCAGCAGAT
     6721 CGCGTGCATC GGAGGCCCTC CCAACATCTG CCTGGACCGT CTGGAAAACT GGATAACTTC
     6781 ATTAGCAGAA TCTCAACTTC AGACCCGCCA ACAAATTAAG AAACTGGAGG AGCTGCAGCA
     6841 GAAAGTGTCC TACAAGGGCG ACCCTATCGT GCAGCACCGG CCCATGCTGG AGGAGAGGAT
     6901 CGTGGAGCTG TTCAGAAACT TAATGAAGAG TGCCTTCGTG GTGGAGCGGC AGCCCTGCAT
     6961 GCCCATGCAC CCGGACCGGC CCTTAGTCAT CAAGACTGGT GTCCAGTTTA CCACGAAAGT
     7021 CAGGTTGCTG GTCAAATTTC CTGAGTTGAA TTATCAGCTT AAAATTAAAG TGTGCATTGA
     7081 TAAAGACTCT GGGGATGTTG CTGCCCTCAG AGGGTCTCGG AAATTTAACA TTCTGGGCAC
     7141 GAACACAAAA GTGATGAACA TGGAGGAGTC TAACAACGGC AGCCTGTCTG CAGAGTTCAA
     7201 GCACCTGACC CTTAGGGAGC AGAGATGTGG GAATGGAGGC CGTGCCAATT GTGATGCCTC
     7261 CTTGATCGTG ACTGAGGAGC TGCACCTGAT CACCTTCGAG ACTGAGGTGT ACCACCAAGG
     7321 CCTCAAGATT GACCTAGAGA CCCACTCCTT GCCAGTTGTG GTGATCTCCA ACATCTGTCA
     7381 GATGCCAAAT GCTTGGGCAT CAATCCTGTG GTATAACATG CTGACCAATA ACCCCAAGAA
     7441 CGTGAACTTC TTCACTAAGC CGCCAATTGG AACCTGGGAC CAAGTGGCCG AGGTGCTCAG
     7501 CTGGCAGTTC TCGTCCACCA CCAAGCGGGG GCTGAGCATC GAGCAGCTGA CAACGCTGGC
     7561 TGAGAAGCTC CTAGGGCCTG GTGTGAACTA CTCAGGGTGT CAGATCACAT GGGCTAAATT
     7621 CTGCAAAGAA AAcatggctg gcaagggctt ctccttctgg gtctggctag acaatatcat
     7681 cgaccttgtg AAAAAGTATA TCTTGGCCCT TTGGAATGAA GGGTACATCA TGGGTTTCAT
     7741 CAGCAAGGAG CGGGAGCGGG CCATCCTAAG CACAAAGCCC CCGGGCACCT TCCTACTGCG
     7801 CTTCAGCGAG AGCAGCAAAG AAGGAGGGGT CACTTTCACT TGGGTGGAAA AGGACATCAG
     7861 TGGCAAGACC CAGATCCAGT CTGTAGAGCC ATACACCAAG CAGCAGCTGA ACAACATGTC
     7921 ATTTGCTGAA ATCATCATGG GCTATAAGAT CATGGATGCG ACCAACATCC TGGTGTCTCC
     7981 ACTTGTCTAC CTCTACCCCG ACATTCCCAA GGAGGAGGCA TTTGGAAAGT ACTGTAGGCC
     8041 CGAGAGCCAG GAGCACCCCG AAGCCGACCC AGGTAGTGCT GCCCCGTACC TGAAGACCAA
     8101 GTTCATCTGT GTGACACCAA CGACCTGCAG CAATACCATT GACCTGCCGA TGTCCCCCCG
     8161 CACTTTAGAT TCATTGATGC AGTTTGGAAA TAACGGTGAA GGTGCTGAGC CCTCAGCAGG
     8221 AGGGCAGTTT GAGTCGCTCA CGTTTGACAT GGATCTGACC TCGGAGTGTG CTACCTCCCC
     8281 CATGgagggc agaggaagtc tcctaacatg cggtgacgtg gaggagaatc ctggcccaat
     8341 gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa
     8401 ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat
     8461 gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag
     8521 cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa
     8581 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgagg acgggggcgt
     8641 gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat
     8701 cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     8761 ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc
     8821 cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat atagatccaa
     8881 gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag
     8941 aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata
     9001 ctgcgacctc cctagcaaac tggggcacaa gcttaattga aatcaacctc tggattacaa
     9061 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     9121 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     9181 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     9241 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     9301 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     9361 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     9421 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     9481 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     9541 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     9601 tcggatctcc ctttgggccg cctccccgc
//