LOCUS       Exported                8883 bp ds-DNA     circular SYN 25-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     187..206
                     /label=T7 Promoter
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     403..425
                     /label=414_gibson_gvpB_fw
     primer_bind     complement(424..448)
                     /label=368_B_backbone1_rv_tile1
     primer_bind     424..448
                     /label=372_B_tile1_fw
     primer_bind     complement(424..448)
                     /label=404_mega_bb2_rv
     misc_feature    439..447
                     /label=RBS
     CDS             453..719
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     misc_feature    528..554
                     /label=beta-strand1
     primer_bind     complement(555..590)
                     /label=369_B_backbone1_rv_tile2
     primer_bind     555..584
                     /label=374_B_tile2_fw
     primer_bind     591..623
                     /label=370_B_backbone2_fw_tile1
     primer_bind     complement(598..623)
                     /label=373_B_tile1_rv
     primer_bind     complement(626..643)
                     /label=399_B_V68C_KLD_rv_v2
     primer_bind     complement(628..645)
                     /label=395_B_V68C_KLD_rv
     primer_bind     657..679
                     /label=401_mega_V68C_G82L_bb1_fw
     primer_bind     704..738
                     /label=400_mega_V68C_only_bb1_fw
     primer_bind     complement(705..729)
                     /label=415_gibson_gvpB_rv
     primer_bind     726..751
                     /label=371_B_backbone2_fw_tile2
     primer_bind     complement(729..754)
                     /label=375_B_tile2_rv
     primer_bind     742..767
                     /label=Bm1seq
     primer_bind     complement(759..779)
                     /label=450_GL312_KLD_rv
     misc_feature    780..788
                     /label=RBS
     CDS             795..1061
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(894..909)
                     /label=352_gvpR_rv1
     primer_bind     1020..1040
                     /label=Q5_008
     primer_bind     complement(1057..1074)
                     /label=456_GL315_KLD_rv
     primer_bind     1062..1079
                     /label=449_GL312_KLD_fw
     misc_feature    1075..1083
                     /label=RBS
     CDS             1090..2016
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1120..1145
                     /label=Bm1.1seqF
     misc_feature    1152
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     complement(1190..1205)
                     /label=GvpN_rv1
     primer_bind     1522..1541
                     /label=Bm2seqF
     primer_bind     1962..1988
                     /label=Q5_010
     primer_bind     complement(1988..2015)
                     /label=Q5_007
     primer_bind     2011..2030
                     /label=455_GL315_KLD_fw
     misc_feature    2017..2025
                     /label=RBS
     CDS             2031..2798
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     complement(2088..2105)
                     /label=GvpF_rv1
     primer_bind     2264..2291
                     /label=Bm3seqF
     primer_bind     2757..2815
                     /label=ESq5mut005
     primer_bind     complement(2771..2798)
                     /label=ESgibson019
     misc_feature    2788..2796
                     /label=RBS
     CDS             2800..3066
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     complement(2998..3014)
                     /label=GvpG_rv1
     primer_bind     3012..3039
                     /label=Bm4seqF
     primer_bind     3022..3081
                     /label=ESq5mut007
     misc_feature    3053..3061
                     /label=RBS
     CDS             3067..3876
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3137
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3206
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3656
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3787..3813
                     /label=Bm5seqF
     misc_feature    3878..3886
                     /label=RBS
     CDS             3894..4181
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(3966..3982)
                     /label=GvpS_rv1
     primer_bind     complement(4017..4045)
                     /label=V S-K fix r
     primer_bind     4030..4063
                     /label=F S-K fix f
     RBS             4120..4128
                     /label=RBS for K
     misc_feature    4216^4217
                     /label=SK
     misc_feature    4238..4246
                     /label=RBS
     CDS             4253..4537
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4517..4545)
                     /label=F S-K fix r
     primer_bind     4530..4552
                     /label=V S-K fix f
     misc_feature    4547..4555
                     /label=RBS
     CDS             4559..4861
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4684
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4690..4711
                     /label=Bm6seqF
     primer_bind     complement(4858..4883)
                     /label=452_GL313_KLD_rv
     misc_feature    4884..4892
                     /label=RBS
     CDS             4898..5776
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4919..4945
                     /label=Bm6.1seq
     primer_bind     complement(4955..4973)
                     /label=GvpT_rv1
     misc_feature    5222
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5285
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5359..5385
                     /label=Bm7seqF
     primer_bind     complement(5762..5783)
                     /label=454_GL314_KLD_rv
     primer_bind     5777..5797
                     /label=451_GL313_KLD_fw
     misc_feature    5784..5792
                     /label=RBS
     CDS             5800..6216
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     complement(5857..5874)
                     /label=GvpU_rv1
     primer_bind     6028..6043
                     /label=GvpU_fw1
     primer_bind     6139..6163
                     /label=GvpUseqF
     primer_bind     6217..6234
                     /label=453_GL314_KLD_fw
     primer_bind     6361..6384
                     /label=390_A1serr_bb1_fw_4-frag
     primer_bind     6369..6394
                     /label=353_AC_backbone1_fw
     primer_bind     complement(6371..6394)
                     /label=393_A1serr_bb3_rv_4-frag
     primer_bind     6423..6443
                     /label=418_gib_GL302_fw
     primer_bind     complement(6423..6442)
                     /label=Gibson022
     primer_bind     6423..6441
                     /label=422_gib_bck_fw
     primer_bind     6423..6441
                     /label=Gibson020
     primer_bind     6423..6441
                     /label=Q5_002
     primer_bind     6423..6441
                     /label=Q5_006
     primer_bind     6423..6441
                     /label=Q5mut011
     primer_bind     6423..6441
                     /label=Q5mut011 (1)
     primer_bind     complement(6429..6450)
                     /label=354_AC_B_backbone2_rv
     primer_bind     complement(6470..6489)
                     /label=T7 Terminator
     primer_bind     complement(6471..6489)
                     /label=Eurofins_T7term
     terminator      6486..6533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6600..6691
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6620..6640)
                     /label=AmpR_rv1
     RBS             6681..6686
                     /label=Emopec_63.3%
     CDS             6692..7552
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7626..8214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8147..8162
                     /label=ori_fw1
     primer_bind     8315..8334
                     /label=Eurofins_VR
     misc_feature    8401..8540
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8572..8592)
                     /label=Eurofins_pexF
     primer_bind     8626..8648
                     /label=pGEX 3'
     primer_bind     8630..8648
                     /label=Eurofins_Gex-Reverse (formerly pGexR)
     CDS             complement(8642..8833)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
       61 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
      121 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ggcaccatgc atcgatcccg
      181 cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga gatagcttag
      241 cgatgtgaaa gaagctggag aaaactttca agaaaaagtt tcttcttcag acgataaaga
      301 gaagtcaaaa aataagcgga aaataaaagg cgtaaatcag attaaaaaag caacggatat
      361 taaaagctct actaagatta aaagttctaa tgatattaaa tcatctacag atttaaaaac
      421 aatgggatca taagcgaaag gagaaatgag atatgtctat tcaaaaaagt actaatagtt
      481 caagtttagc agaagtcatt gaccgtattt tagataaagg aattgttatt gatgcttttg
      541 caagagtttc tgttgtagga attgaaattt taacgattga agcgcgagtg gttattgcca
      601 gtgttgatac atggttacgc tatgcagaag cagtagggct tcttcgtgac gacgtagaag
      661 aaaacggtct tcctgaacgt tcaaattcaa gtgaagggca gccgcgtttt agtatttaat
      721 atgaagtggt atggaacctt ccttttttga gggtggttcc agcttttaga tctaactatt
      781 ggaggctact aaaaatggaa attaaaaaaa ttatgcaagc cgtgaacgac tttttcggtg
      841 aacacgtagc tcctcctcat aaaattacct cggtggaagc tactgaagat gaaggttgga
      901 gagttattgt tgaagtcatt gaagaacgag aatatatgaa aaaatacgcc aaagatgaaa
      961 tgctcggaac gtacgagtgc tttgtaaata aagaaaaaga agtcatttca ttcaaacgac
     1021 tcgacgtcag atatagaagc gccattggca ttgaagcata aacgataaga tggcaggagg
     1081 aacgtaaaaa tgaccgtctt aacagacaaa aggaaaaaag gcagtggagc ttttatacaa
     1141 gatgacgaga caaaagaggt tctttcaaga gcgctgagct atttaaaatc cggctattcc
     1201 attcatttta caggtcctgc cggcggaggc aaaacctctt tagcgcgagc gcttgctaaa
     1261 aagagaaagc gtcctgtaat gctgatgcac gggaatcacg agctcaacaa caaagattta
     1321 attggcgatt ttacgggata cacgagcaaa aaagtaatcg accagtacgt tcgttctgtc
     1381 tataaaaaag atgaacaggt gagtgaaaac tggcaggatg gccgattgct tgaagctgta
     1441 aaaaatggct atacgctgat ttacgacgaa tttactcgtt ctaagcctgc gacgaataat
     1501 atctttctat cgatattaga agaaggcgtg ctgccgctgt atggagtaaa aatgaccgat
     1561 ccttttgtgc gcgtgcatcc cgatttccgc gtcatcttca caagcaatcc agctgagtat
     1621 gccggcgtat atgatacgca agatgcgctt ctcgacaggt taattaccat gtttattgat
     1681 tataaagaca tcgacagaga gacagcgatt ttaacggaga aaacggacgt agaagaagat
     1741 gaagcgcgca caattgtaac gctcgtagca aacgtgcgaa accgctctgg agacgaaaac
     1801 agcagcggac ttagcctgcg ggcttcgctt atgatcgcta cccttgccac gcagcaagac
     1861 attcctatcg atggaagtga cgaagatttt caaacgttat gtatcgatat tttgcatcat
     1921 ccgcttacca aatgtttgga tgaagaaaat gcaaaaagca aagccgaaaa aatcatttta
     1981 gaagaatgta agaatataga cactgaagaa aagtaaagga gcttgaaaac atgagtgaaa
     2041 caaacgaaac aggtatttat atttttagcg ccattcaaac ggataaagac gaagaatttg
     2101 gcgccgtgga agtagaagga acaaaagctg aaacattttt gattcgctac aaagacgcgg
     2161 ctatggtagc agctgaagta ccgatgaaaa tttatcatcc taatcgccaa aatttattaa
     2221 tgcatcaaaa cgcagtagca gcgattatgg acaagaacga tacggttatt ccaatcagct
     2281 ttgggaatgt attcaaatca aaagaagacg taaaagttct tttggaaaac ctttatccgc
     2341 agtttgaaaa gctgtttcca gcgatcaaag gaaaaattga agtcggttta aaagtaattg
     2401 ggaaaaaaga atggcttgag aaaaaagtaa acgaaaatcc tgaacttgag aaagtatcag
     2461 catccgtaaa aggaaaatca gaagcagccg gttattatga gcgtattcaa cttggaggaa
     2521 tggctcaaaa gatgtttact tccctgcaaa aagaagtcaa gacagatgtg ttttctccgc
     2581 ttgaagaagc agcggaagca gcaaaagcaa atgagccaac gggcgaaacg atgcttttaa
     2641 acgcgtcttt cttaattaac cgagaagatg aagcgaagtt tgatgaaaaa gtaaatgaag
     2701 cgcatgaaaa ctggaaagac aaagccgatt ttcattacag cggtccttgg cctgcttata
     2761 attttgtgaa cattcgccta aaagtagaag agaaataacg tgcttcacaa attagtaacc
     2821 gcacccatta accttgtagt gaaaatcggc gaaaaagtac aggaagaagc tgataaacag
     2881 ctatatgacc ttccgacgat tcagcaaaag ctcattcagc ttcaaatgat gtttgagctt
     2941 ggtgaaattc cagaagaagc gtttcaagaa aaagaagatg aattgttaat gaggtacgaa
     3001 attgcgaaac gcagagaaat tgaacaatgg gaagagctaa cacaaaaaag aaatgaggaa
     3061 tcctagatgg gagaattact gtatttatac ggtttaattc caacaaaaga agcagcagcc
     3121 atagagccgt ttccatctta taaggggttt gacggagaac attcactgta cccaattgcg
     3181 tttgatcagg tgacggctgt agtttctaag ctggatgctg acacctattc agaaaaagtg
     3241 attcaagaaa aaatggagca ggatatgagc tggctgcagg aaaaagcatt tcatcatcac
     3301 gaaacggtag ccgctttgta cgaagaattt acgatcattc cattaaaatt ttgcaccatt
     3361 tataaaggtg aagaaagtct gcaggcagct attgagatta acaaagaaaa gatagagaat
     3421 tcactgacgc tgcttcaagg aaatgaagag tggaatgtga aaatttactg tgatgataca
     3481 gagcttaaaa aaggaatcag cgaaacgaat gaaagcgtga aagcgaaaaa acaagaaatt
     3541 agtcacttat caccaggaag acagtttttt gaaaagaaaa aaatagatca gctgattgaa
     3601 aaagaattag agcttcacaa aaacaaagtg tgtgaagaga tacatgacaa gctaaaagaa
     3661 ttatcgcttt atgactctgt taaaaagaat tggagcaaag acgtaactgg cgcagctgaa
     3721 cagatggcgt ggaacagcgt gtttcttctc ccgtctctgc agattactaa gttcgtaaac
     3781 gaaatagaag agcttcagca aaggcttgaa aataaaggct ggaagtttga agtgacggga
     3841 ccatggccgc cctatcattt ctcgagcttt gcgtaaagtg aggaattaac attatgtctc
     3901 ttaaacaatc catggagaat aaagatattg ctcttattga tattttagat gtcattttag
     3961 ataaaggagt cgccattaaa ggagacttaa tcatttccat agctggcgtc gatttagtgt
     4021 atttggattt gcgggtgctt atttcttcgg ttgaaacgct tgtgcaagca aaagaaggaa
     4081 atcacaaacc aatcacttct gaacaatttg ataaacaaaa agaagagctg atggacgcta
     4141 cgggccagcc tagtaagtgg acgaaccctc taggttcgtg acccttgcga gcagtcgtat
     4201 gctttctcga attccgagcg gttaagcgtg acagtccagg aggaattaat agatgcaacc
     4261 ggtcagccaa gcaaatggac gaatccactt ggatcctgat caagctgaac aaggcttagc
     4321 gcagcttgtg atgacagtta ttgagctatt gaggcaaatc gttgaacgtc atgccatgag
     4381 gcgggtggag ggtggaacgt tgacggacga acaaattgaa aacttaggaa ttgcactaat
     4441 gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc ggtctggatg cagaagattt
     4501 aaatattgat cttggcccgc taggcagcct gctttaagcg gtcagtagga ggaacagtat
     4561 ggcagtcgaa cataatatgc agtcaagtac gattgtagat gtgctcgaaa agattttgga
     4621 taaaggagtc gttatagcgg gggacatcac cgtaggaatt gcagatgtcg agctattaac
     4681 gataaagatc cgcttgattg tggcttcggt tgataaggca aaagaaatcg gcatggactg
     4741 gtgggaaaat gatccgtatc tcagttcaaa aggagccaat aacaaagcgc tcgaagaaga
     4801 aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa aaaatagaaa cgaaacgtta
     4861 aaaactgtac gctacttaaa aaatggaggg atttacaatg gcaactgaaa caaaattaga
     4921 taacacacag gcagaaaaca aggaaaataa aaatgcggaa aacggttcaa aagaaaagaa
     4981 cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc aaacgagcgg tagcaggagg
     5041 catcatcggt gcaacgattg gatatgtatc gactcctgaa aatcgaaaaa gtctccttga
     5101 ccgcattgat acagacgaat taaaaagcaa agcatctgat ttaggaacaa aggtaaaaga
     5161 aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg ggaagcttgt ttaaaaaaga
     5221 taaagataaa tcaaaagatg atgaagaaaa cgtaaattct tctagtagcg aaacagaaga
     5281 cgataacgtt caagagtacg acgagttaaa agaagaaaat caaactcttc aagatcgctt
     5341 atcacagctt gaagaaaaaa tgaacatgct tgttgagctt agcctcaata aaaatcaaga
     5401 cgaagaagcg gaagatacag attccgacga agaagagaac gatgagaacg atgaaaacga
     5461 tgaaaacgag caggacgatg aaaacgaaga agaaacatct aagccacgta aaaaggataa
     5521 aaaagaagct gaggaagaag aaagtgaaag tgacgaagac agcgaggaag aagaggaaga
     5581 ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa gaagaagacg aagatgaaag
     5641 cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca gctaaaaaat caaaaaatac
     5701 aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca acatctcttt ctagtgaaga
     5761 cgatacaaca gcctaagacg taaaggagga aagaaagaca tgagtacagg cccttctttt
     5821 tcaactaaag acaatacgct tgaatacttt gtgaaagctt ctaataaaca cggcttttca
     5881 cttgatattt cattaaatgt aaacggcgct gtgatttccg gtaccatgat ttcagcaaaa
     5941 gaatactttg attacttaag cgaaacgttt gaagaaggca gtgaagtggc tcaggcgcta
     6001 agcgaacaat tctctttagc aagcgaagcg agcgaatcaa acggagaagc agaagcccat
     6061 tttattcatt tgaaaaatac aaagatttac tgtggagaca gtaaatctac tccttctaaa
     6121 ggcaaaatct tttggagagg gaaaatagca gaagtagacg ggtttttctt aggaaagatt
     6181 tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa cggcggggtg attgccccgc
     6241 cgttttttag tgatgtgatg agatgtgcag cttctttttt cgatacatga gcggagtcat
     6301 cttcatttct tttcgaaact gattaataaa atagcttgta ctattaaacc ctacttgata
     6361 ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg gattcgcgaa cgcgtctcga
     6421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     6481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     6541 gaggaactat atccgactag tagcccgcct aatgagcggg ctttttttta attcccctat
     6601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctcgtgaa
     6781 agtaaaagac gcagaggacc aattgggggc acgagtggga tacatagaac tggacttgaa
     6841 tagcggtaaa atccttgaga gttttcgccc tgaagagcgt tttccaatga tgagcacttt
     6901 caaagttctg ctatgtggag cagtattatc ccgtgtagat gcggggcaag agcaactcgg
     6961 acgacgaata cactattcgc agaatgactt ggttgaatac tccccagtga cagaaaagca
     7021 ccttacggac ggaatgacgg taagagaatt atgtagtgcc gccataacga tgagtgataa
     7081 cactgcggcg aacttacttc tgacaaccat cggtggaccg aaggaattaa ccgctttttt
     7141 gcacaatatg ggagaccatg taactcgcct tgaccgttgg gaaccagaac tgaatgaagc
     7201 cataccaaac gacgagcgag acaccacaat gcctgcggca atggcaacaa cattacgcaa
     7261 actattaact ggcgaactac ttactctggc ttcacggcaa caattaatag actggcttga
     7321 agcggataaa gttgcaggac cactactgcg ttcggcactt cctgctggct ggtttattgc
     7381 tgataaatct ggggcaggag agcgtggttc acggggtatc attgccgcac ttggaccaga
     7441 tggtaagcct tcccgtatcg tagttatcta cacgacgggt agtcaggcaa ctatggacga
     7501 acgaaataga cagattgctg aaataggggc ttcactgatt aagcattggt aaaccgatac
     7561 aattaaaggc tccttttgga gccttttttt ttggacggac cggtagaaaa gatcaaagga
     7621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7741 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     7801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8221 agcaacgcgg ccctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8281 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8341 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8401 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
     8461 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
     8521 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     8581 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     8641 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
     8701 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
     8761 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
     8821 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc
     8881 gat
//
