LOCUS       Exported                2945 bp ds-DNA     circular SYN 13-FEB-2024
DEFINITION  tig00000001.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(165..754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(785..824)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     misc_feature    828..1688
                     /label=AmpR (beta lactamase)
     promoter        complement(1689..1780)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(1847..1894)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2237..2255)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2492..2683
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    2785..2924
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
       61 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      601 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      721 cagcagatta cgcgcagaaa aaaaaggatc tcaagaagat cctttgatct tttctaccgg
      781 tccgtccaaa aaaaaaggct ccaaaaggag cctttaattg tatcggttta ccaatgctta
      841 atcagtgaag cccctatttc agcaatctgt ctatttcgtt cgtccatagt tgcctgacta
      901 cccgtcgtgt agataactac gatacgggaa ggcttaccat ctggtccaag tgcggcaatg
      961 ataccccgtg aaccacgctc tcctgcccca gatttatcag caataaacca gccagcagga
     1021 agtgccgaac gcagtagtgg tcctgcaact ttatccgctt caagccagtc tattaattgt
     1081 tgccgtgaag ccagagtaag tagttcgcca gttaatagtt tgcgtaatgt tgttgccatt
     1141 gccgcaggca ttgtggtgtc tcgctcgtcg tttggtatgg cttcattcag ttctggttcc
     1201 caacggtcaa ggcgagttac atggtctccc atattgtgca aaaaagcggt taattccttc
     1261 ggtccaccga tggttgtcag aagtaagttc gccgcagtgt tatcactcat cgttatggcg
     1321 gcactacata attctcttac cgtcattccg tccgtaaggt gcttttctgt cactggggag
     1381 tattcaacca agtcattctg cgaatagtgt attcgtcgtc cgagttgctc ttgccccgca
     1441 tctacacggg ataatactgc tccacatagc agaactttga aagtgctcat cattggaaaa
     1501 cgctcttcag ggcgaaaact ctcaaggatt ttaccgctat tcaagtccag ttctatgtat
     1561 cccactcgtg cccccaattg gtcctctgcg tcttttactt tcacgagcgt ttctgggtga
     1621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1681 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1741 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggaatt aaaaaaaagc
     1801 ccgctcatta ggcgggctac tagtcggata tagttcctcc tttcagcaaa aaacccctca
     1861 agacccgttt agaggcccca aggggttatg ctagttattg ctcagcggtg gcagcagcca
     1921 actcagcttc ctttcgggct ttgttagcag ccggatctct cgagacgcgt tcgcgaatcc
     1981 ggactgcagc aagcagaagc aaatgtgacg atgatcccat tgtttttaaa tctgtagatg
     2041 atttaatatc attagaactt ttaatcttag tagagctttt aatatccgtt gcttttttaa
     2101 tctgatttac gccttttatt ttccgcttat tttttgactt ctctttatcg tctgaagaag
     2161 aaactttttc ttgaaagttt tctccagctt ctttcacatc gctaagctat ctctagaggg
     2221 aaaccgttgt ggtctcccta tagtgagtcg tattaatttc gcgggatcga tgcatggtgc
     2281 caacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
     2341 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
     2401 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccat
     2461 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
     2521 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     2581 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     2641 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     2701 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     2761 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     2821 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattgcggt
     2881 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     2941 cgctc
//
