LOCUS       Exported                8073 bp ds-DNA     circular SYN 10-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        36..127
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(56..76)
                     /label=AmpR_rv1
     RBS             117..122
                     /label=Emopec_63.3%
     CDS             128..988
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1062..1650
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1583..1598
                     /label=ori_fw1
     primer_bind     1751..1770
                     /label=Eurofins_VR
     misc_feature    1837..1976
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2008..2028)
                     /label=Eurofins_pexF
     primer_bind     2062..2084
                     /label=pGEX 3'
     primer_bind     2066..2084
                     /label=Eurofins_Gex-Reverse (formerly pGexR)
     CDS             complement(2078..2269)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2506..2525
                     /label=T7 Promoter
     promoter        2506..2524
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2722..2744
                     /label=414_gibson_gvpB_fw
     primer_bind     complement(2743..2767)
                     /label=368_B_backbone1_rv_tile1
     primer_bind     2743..2767
                     /label=372_B_tile1_fw
     primer_bind     complement(2743..2767)
                     /label=404_mega_bb2_rv
     misc_feature    2758..2766
                     /label=RBS
     CDS             2772..3038
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     misc_feature    2847..2873
                     /label=beta-strand1
     primer_bind     complement(2874..2909)
                     /label=369_B_backbone1_rv_tile2
     primer_bind     2874..2903
                     /label=374_B_tile2_fw
     primer_bind     2910..2942
                     /label=370_B_backbone2_fw_tile1
     primer_bind     complement(2917..2942)
                     /label=373_B_tile1_rv
     primer_bind     complement(2945..2962)
                     /label=399_B_V68C_KLD_rv_v2
     primer_bind     complement(2947..2964)
                     /label=395_B_V68C_KLD_rv
     primer_bind     2976..2998
                     /label=401_mega_V68C_G82L_bb1_fw
     primer_bind     3023..3057
                     /label=400_mega_V68C_only_bb1_fw
     primer_bind     complement(3024..3048)
                     /label=415_gibson_gvpB_rv
     primer_bind     3045..3070
                     /label=371_B_backbone2_fw_tile2
     primer_bind     complement(3048..3073)
                     /label=375_B_tile2_rv
     primer_bind     3061..3086
                     /label=Bm1seq
     primer_bind     complement(3078..3098)
                     /label=450_GL312_KLD_rv
     misc_feature    3099..3107
                     /label=RBS
     CDS             3114..3380
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(3213..3228)
                     /label=352_gvpR_rv1
     primer_bind     3339..3359
                     /label=Q5_008
     primer_bind     complement(3376..3393)
                     /label=456_GL315_KLD_rv
     primer_bind     3381..3398
                     /label=449_GL312_KLD_fw
     misc_feature    3394..3402
                     /label=RBS
     CDS             3409..4335
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     3439..3464
                     /label=Bm1.1seqF
     misc_feature    3471
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     complement(3509..3524)
                     /label=GvpN_rv1
     primer_bind     3841..3860
                     /label=Bm2seqF
     primer_bind     4281..4307
                     /label=Q5_010
     primer_bind     complement(4307..4334)
                     /label=Q5_007
     primer_bind     4330..4349
                     /label=455_GL315_KLD_fw
     misc_feature    4336..4344
                     /label=RBS
     CDS             4350..5117
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     complement(4407..4424)
                     /label=GvpF_rv1
     primer_bind     4583..4610
                     /label=Bm3seqF
     primer_bind     5076..5134
                     /label=ESq5mut005
     primer_bind     complement(5090..5117)
                     /label=ESgibson019
     misc_feature    5107..5115
                     /label=RBS
     CDS             5119..5385
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     complement(5317..5333)
                     /label=GvpG_rv1
     primer_bind     5331..5358
                     /label=Bm4seqF
     primer_bind     5341..5386
                     /label=ESq5mut007
     primer_bind     complement(5353..5409)
                     /label=G dL r
     misc_feature    5372..5380
                     /label=RBS
     primer_bind     5386..5420
                     /label=G dL f
     misc_feature    5387..5395
                     /label=RBS
     CDS             5403..5690
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(5475..5491)
                     /label=GvpS_rv1
     primer_bind     complement(5526..5554)
                     /label=V S-K fix r
     primer_bind     5539..5572
                     /label=F S-K fix f
     RBS             5629..5637
                     /label=RBS for K
     misc_feature    5725^5726
                     /label=SK
     misc_feature    5747..5755
                     /label=RBS
     CDS             5762..6046
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(6026..6054)
                     /label=F S-K fix r
     primer_bind     6039..6061
                     /label=V S-K fix f
     misc_feature    6056..6064
                     /label=RBS
     CDS             6068..6370
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    6193
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6199..6220
                     /label=Bm6seqF
     primer_bind     complement(6367..6392)
                     /label=452_GL313_KLD_rv
     primer_bind     complement(6374..6405)
                     /label=G dTU r
     misc_feature    6393..6401
                     /label=RBS
     CDS             6407..7285
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     6428..6454
                     /label=Bm6.1seq
     primer_bind     complement(6464..6482)
                     /label=GvpT_rv1
     misc_feature    6731
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    6794
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6868..6894
                     /label=Bm7seqF
     primer_bind     complement(7271..7292)
                     /label=454_GL314_KLD_rv
     primer_bind     7286..7306
                     /label=451_GL313_KLD_fw
     misc_feature    7293..7301
                     /label=RBS
     CDS             7309..7725
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     complement(7366..7383)
                     /label=GvpU_rv1
     primer_bind     7537..7552
                     /label=GvpU_fw1
     primer_bind     7648..7672
                     /label=GvpUseqF
     primer_bind     7721..7743
                     /label=G dTU f
     primer_bind     complement(7721..7737)
                     /label=G dTU r
     primer_bind     7726..7743
                     /label=453_GL314_KLD_fw
     primer_bind     7744..7758
                     /label=G dTU r
     primer_bind     7870..7893
                     /label=390_A1serr_bb1_fw_4-frag
     primer_bind     7878..7903
                     /label=353_AC_backbone1_fw
     primer_bind     complement(7880..7903)
                     /label=393_A1serr_bb3_rv_4-frag
     primer_bind     7932..7952
                     /label=418_gib_GL302_fw
     primer_bind     complement(7932..7951)
                     /label=Gibson022
     primer_bind     7932..7950
                     /label=422_gib_bck_fw
     primer_bind     7932..7950
                     /label=Gibson020
     primer_bind     7932..7950
                     /label=Q5_002
     primer_bind     7932..7950
                     /label=Q5_006
     primer_bind     7932..7950
                     /label=Q5mut011
     primer_bind     7932..7950
                     /label=Q5mut011 (1)
     primer_bind     complement(7938..7959)
                     /label=354_AC_B_backbone2_rv
     primer_bind     complement(7979..7998)
                     /label=T7 Terminator
     primer_bind     complement(7980..7998)
                     /label=Eurofins_T7term
     terminator      7995..8042
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ccgcctaatg agcgggcttt tttttaattc ccctatttgt ttatttttct aaatacattc
       61 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      181 ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta aaagacgcag aggaccaatt
      241 gggggcacga gtgggataca tagaactgga cttgaatagc ggtaaaatcc ttgagagttt
      301 tcgccctgaa gagcgttttc caatgatgag cactttcaaa gttctgctat gtggagcagt
      361 attatcccgt gtagatgcgg ggcaagagca actcggacga cgaatacact attcgcagaa
      421 tgacttggtt gaatactccc cagtgacaga aaagcacctt acggacggaa tgacggtaag
      481 agaattatgt agtgccgcca taacgatgag tgataacact gcggcgaact tacttctgac
      541 aaccatcggt ggaccgaagg aattaaccgc ttttttgcac aatatgggag accatgtaac
      601 tcgccttgac cgttgggaac cagaactgaa tgaagccata ccaaacgacg agcgagacac
      661 cacaatgcct gcggcaatgg caacaacatt acgcaaacta ttaactggcg aactacttac
      721 tctggcttca cggcaacaat taatagactg gcttgaagcg gataaagttg caggaccact
      781 actgcgttcg gcacttcctg ctggctggtt tattgctgat aaatctgggg caggagagcg
      841 tggttcacgg ggtatcattg ccgcacttgg accagatggt aagccttccc gtatcgtagt
      901 tatctacacg acgggtagtc aggcaactat ggacgaacga aatagacaga ttgctgaaat
      961 aggggcttca ctgattaagc attggtaaac cgatacaatt aaaggctcct tttggagcct
     1021 ttttttttgg acggaccggt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1081 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1141 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1201 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1261 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1321 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1381 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1441 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1501 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1561 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1621 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggccct ttttacggtt
     1681 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1741 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1801 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     1861 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc
     1921 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     1981 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2041 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2101 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2161 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2221 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2281 tgtaaggggg atttctgttc atggggtaat gataccgatg aaacgagaga ggatgctcac
     2341 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     2401 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     2461 taatacagat gtaggtgttg gcaccatgca tcgatcccgc gaaattaata cgactcacta
     2521 tagggagacc acaacggttt ccctctagag atagcttagc gatgtgaaag aagctggaga
     2581 aaactttcaa gaaaaagttt cttcttcaga cgataaagag aagtcaaaaa ataagcggaa
     2641 aataaaaggc gtaaatcaga ttaaaaaagc aacggatatt aaaagctcta ctaagattaa
     2701 aagttctaat gatattaaat catctacaga tttaaaaaca atgggatcat aagcgaaagg
     2761 agaaatgaga tatgtctatt caaaaaagta ctaatagttc aagtttagca gaagtcattg
     2821 accgtatttt agataaagga attgttattg atgcttttgc aagagtttct gttgtaggaa
     2881 ttgaaatttt aacgattgaa gcgcgagtgg ttattgccag tgttgataca tggttacgct
     2941 atgcagaagc agtagggctt cttcgtgacg acgtagaaga aaacggtctt cctgaacgtt
     3001 caaattcaag tgaagggcag ccgcgtttta gtatttaata tgaagtggta tggaaccttc
     3061 cttttttgag ggtggttcca gcttttagat ctaactattg gaggctacta aaaatggaaa
     3121 ttaaaaaaat tatgcaagcc gtgaacgact ttttcggtga acacgtagct cctcctcata
     3181 aaattacctc ggtggaagct actgaagatg aaggttggag agttattgtt gaagtcattg
     3241 aagaacgaga atatatgaaa aaatacgcca aagatgaaat gctcggaacg tacgagtgct
     3301 ttgtaaataa agaaaaagaa gtcatttcat tcaaacgact cgacgtcaga tatagaagcg
     3361 ccattggcat tgaagcataa acgataagat ggcaggagga acgtaaaaat gaccgtctta
     3421 acagacaaaa ggaaaaaagg cagtggagct tttatacaag atgacgagac aaaagaggtt
     3481 ctttcaagag cgctgagcta tttaaaatcc ggctattcca ttcattttac aggtcctgcc
     3541 ggcggaggca aaacctcttt agcgcgagcg cttgctaaaa agagaaagcg tcctgtaatg
     3601 ctgatgcacg ggaatcacga gctcaacaac aaagatttaa ttggcgattt tacgggatac
     3661 acgagcaaaa aagtaatcga ccagtacgtt cgttctgtct ataaaaaaga tgaacaggtg
     3721 agtgaaaact ggcaggatgg ccgattgctt gaagctgtaa aaaatggcta tacgctgatt
     3781 tacgacgaat ttactcgttc taagcctgcg acgaataata tctttctatc gatattagaa
     3841 gaaggcgtgc tgccgctgta tggagtaaaa atgaccgatc cttttgtgcg cgtgcatccc
     3901 gatttccgcg tcatcttcac aagcaatcca gctgagtatg ccggcgtata tgatacgcaa
     3961 gatgcgcttc tcgacaggtt aattaccatg tttattgatt ataaagacat cgacagagag
     4021 acagcgattt taacggagaa aacggacgta gaagaagatg aagcgcgcac aattgtaacg
     4081 ctcgtagcaa acgtgcgaaa ccgctctgga gacgaaaaca gcagcggact tagcctgcgg
     4141 gcttcgctta tgatcgctac ccttgccacg cagcaagaca ttcctatcga tggaagtgac
     4201 gaagattttc aaacgttatg tatcgatatt ttgcatcatc cgcttaccaa atgtttggat
     4261 gaagaaaatg caaaaagcaa agccgaaaaa atcattttag aagaatgtaa gaatatagac
     4321 actgaagaaa agtaaaggag cttgaaaaca tgagtgaaac aaacgaaaca ggtatttata
     4381 tttttagcgc cattcaaacg gataaagacg aagaatttgg cgccgtggaa gtagaaggaa
     4441 caaaagctga aacatttttg attcgctaca aagacgcggc tatggtagca gctgaagtac
     4501 cgatgaaaat ttatcatcct aatcgccaaa atttattaat gcatcaaaac gcagtagcag
     4561 cgattatgga caagaacgat acggttattc caatcagctt tgggaatgta ttcaaatcaa
     4621 aagaagacgt aaaagttctt ttggaaaacc tttatccgca gtttgaaaag ctgtttccag
     4681 cgatcaaagg aaaaattgaa gtcggtttaa aagtaattgg gaaaaaagaa tggcttgaga
     4741 aaaaagtaaa cgaaaatcct gaacttgaga aagtatcagc atccgtaaaa ggaaaatcag
     4801 aagcagccgg ttattatgag cgtattcaac ttggaggaat ggctcaaaag atgtttactt
     4861 ccctgcaaaa agaagtcaag acagatgtgt tttctccgct tgaagaagca gcggaagcag
     4921 caaaagcaaa tgagccaacg ggcgaaacga tgcttttaaa cgcgtctttc ttaattaacc
     4981 gagaagatga agcgaagttt gatgaaaaag taaatgaagc gcatgaaaac tggaaagaca
     5041 aagccgattt tcattacagc ggtccttggc ctgcttataa ttttgtgaac attcgcctaa
     5101 aagtagaaga gaaataacgt gcttcacaaa ttagtaaccg cacccattaa ccttgtagtg
     5161 aaaatcggcg aaaaagtaca ggaagaagct gataaacagc tatatgacct tccgacgatt
     5221 cagcaaaagc tcattcagct tcaaatgatg tttgagcttg gtgaaattcc agaagaagcg
     5281 tttcaagaaa aagaagatga attgttaatg aggtacgaaa ttgcgaaacg cagagaaatt
     5341 gaacaatggg aagagctaac acaaaaaaga aatgaggaat cctagagtga ggaattaaca
     5401 ttatgtctct taaacaatcc atggagaata aagatattgc tcttattgat attttagatg
     5461 tcattttaga taaaggagtc gccattaaag gagacttaat catttccata gctggcgtcg
     5521 atttagtgta tttggatttg cgggtgctta tttcttcggt tgaaacgctt gtgcaagcaa
     5581 aagaaggaaa tcacaaacca atcacttctg aacaatttga taaacaaaaa gaagagctga
     5641 tggacgctac gggccagcct agtaagtgga cgaaccctct aggttcgtga cccttgcgag
     5701 cagtcgtatg ctttctcgaa ttccgagcgg ttaagcgtga cagtccagga ggaattaata
     5761 gatgcaaccg gtcagccaag caaatggacg aatccacttg gatcctgatc aagctgaaca
     5821 aggcttagcg cagcttgtga tgacagttat tgagctattg aggcaaatcg ttgaacgtca
     5881 tgccatgagg cgggtggagg gtggaacgtt gacggacgaa caaattgaaa acttaggaat
     5941 tgcactaatg aacttagaag aaaaaatgga cgagttgaaa gaggtgttcg gtctggatgc
     6001 agaagattta aatattgatc ttggcccgct aggcagcctg ctttaagcgg tcagtaggag
     6061 gaacagtatg gcagtcgaac ataatatgca gtcaagtacg attgtagatg tgctcgaaaa
     6121 gattttggat aaaggagtcg ttatagcggg ggacatcacc gtaggaattg cagatgtcga
     6181 gctattaacg ataaagatcc gcttgattgt ggcttcggtt gataaggcaa aagaaatcgg
     6241 catggactgg tgggaaaatg atccgtatct cagttcaaaa ggagccaata acaaagcgct
     6301 cgaagaagaa aataaaatgc tgcatgagcg gttaaaaacg cttgaagaaa aaatagaaac
     6361 gaaacgttaa aaactgtacg ctacttaaaa aatggaggga tttacaatgg caactgaaac
     6421 aaaattagat aacacacagg cagaaaacaa ggaaaataaa aatgcggaaa acggttcaaa
     6481 agaaaagaac ggttcaaaag caagcaaaac aacaagcagc gggccaatca aacgagcggt
     6541 agcaggaggc atcatcggtg caacgattgg atatgtatcg actcctgaaa atcgaaaaag
     6601 tctccttgac cgcattgata cagacgaatt aaaaagcaaa gcatctgatt taggaacaaa
     6661 ggtaaaagaa aaatcaaaaa gcagcgtggc cagcctgaaa acatctgcgg gaagcttgtt
     6721 taaaaaagat aaagataaat caaaagatga tgaagaaaac gtaaattctt ctagtagcga
     6781 aacagaagac gataacgttc aagagtacga cgagttaaaa gaagaaaatc aaactcttca
     6841 agatcgctta tcacagcttg aagaaaaaat gaacatgctt gttgagctta gcctcaataa
     6901 aaatcaagac gaagaagcgg aagatacaga ttccgacgaa gaagagaacg atgagaacga
     6961 tgaaaacgat gaaaacgagc aggacgatga aaacgaagaa gaaacatcta agccacgtaa
     7021 aaaggataaa aaagaagctg aggaagaaga aagtgaaagt gacgaagaca gcgaggaaga
     7081 agaggaagat tctcgctcaa acaaaaaaaa taaaaaagta aaaacagaag aagaagacga
     7141 agatgaaagc gaagaagaaa aaaaggaagc gaaaccaaaa aagtcaacag ctaaaaaatc
     7201 aaaaaataca aaagcaaaga aaaacacgga cgaagaagat gatgaagcaa catctctttc
     7261 tagtgaagac gatacaacag cctaagacgt aaaggaggaa agaaagacat gagtacaggc
     7321 ccttcttttt caactaaaga caatacgctt gaatactttg tgaaagcttc taataaacac
     7381 ggcttttcac ttgatatttc attaaatgta aacggcgctg tgatttccgg taccatgatt
     7441 tcagcaaaag aatactttga ttacttaagc gaaacgtttg aagaaggcag tgaagtggct
     7501 caggcgctaa gcgaacaatt ctctttagca agcgaagcga gcgaatcaaa cggagaagca
     7561 gaagcccatt ttattcattt gaaaaataca aagatttact gtggagacag taaatctact
     7621 ccttctaaag gcaaaatctt ttggagaggg aaaatagcag aagtagacgg gtttttctta
     7681 ggaaagattt ctgatgcaaa atcaacgagt aaaaagagtt cataaaaaac ggcggggtga
     7741 ttgccccgcc gttttttagt gatgtgatga gatgtgcagc ttcttttttc gatacatgag
     7801 cggagtcatc ttcatttctt ttcgaaactg attaataaaa tagcttgtac tattaaaccc
     7861 tacttgatag gcgacttccg tcacatttgc ttctgcttgc tgcagtccgg attcgcgaac
     7921 gcgtctcgag agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     7981 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     8041 tgctgaaagg aggaactata tccgactagt agc
//
