LOCUS       Exported                8580 bp ds-DNA     circular SYN 10-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        21..112
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(41..61)
                     /label=AmpR_rv1
     RBS             102..107
                     /label=Emopec_63.3%
     CDS             113..973
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1047..1635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1568..1583
                     /label=ori_fw1
     primer_bind     1736..1755
                     /label=Eurofins_VR
     misc_feature    1822..1961
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1993..2013)
                     /label=Eurofins_pexF
     primer_bind     2047..2069
                     /label=pGEX 3'
     primer_bind     2051..2069
                     /label=Eurofins_Gex-Reverse (formerly pGexR)
     CDS             complement(2063..2254)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2491..2510
                     /label=T7 Promoter
     promoter        2491..2509
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2707..2729
                     /label=414_gibson_gvpB_fw
     primer_bind     complement(2728..2752)
                     /label=368_B_backbone1_rv_tile1
     primer_bind     2728..2752
                     /label=372_B_tile1_fw
     primer_bind     complement(2728..2752)
                     /label=404_mega_bb2_rv
     misc_feature    2743..2751
                     /label=RBS
     CDS             2757..3023
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     misc_feature    2832..2858
                     /label=beta-strand1
     primer_bind     complement(2859..2894)
                     /label=369_B_backbone1_rv_tile2
     primer_bind     2859..2888
                     /label=374_B_tile2_fw
     primer_bind     2895..2927
                     /label=370_B_backbone2_fw_tile1
     primer_bind     complement(2902..2927)
                     /label=373_B_tile1_rv
     primer_bind     complement(2930..2947)
                     /label=399_B_V68C_KLD_rv_v2
     primer_bind     complement(2932..2949)
                     /label=395_B_V68C_KLD_rv
     primer_bind     2961..2983
                     /label=401_mega_V68C_G82L_bb1_fw
     primer_bind     3008..3042
                     /label=400_mega_V68C_only_bb1_fw
     primer_bind     complement(3009..3033)
                     /label=415_gibson_gvpB_rv
     primer_bind     3030..3055
                     /label=371_B_backbone2_fw_tile2
     primer_bind     complement(3033..3058)
                     /label=375_B_tile2_rv
     primer_bind     3046..3071
                     /label=Bm1seq
     primer_bind     complement(3063..3083)
                     /label=450_GL312_KLD_rv
     misc_feature    3084..3092
                     /label=RBS
     CDS             3099..3365
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(3198..3213)
                     /label=352_gvpR_rv1
     primer_bind     3324..3344
                     /label=Q5_008
     primer_bind     complement(3361..3378)
                     /label=456_GL315_KLD_rv
     primer_bind     3366..3383
                     /label=449_GL312_KLD_fw
     misc_feature    3379..3387
                     /label=RBS
     CDS             3394..4320
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     3424..3449
                     /label=Bm1.1seqF
     misc_feature    3456
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     complement(3494..3509)
                     /label=GvpN_rv1
     primer_bind     3826..3845
                     /label=Bm2seqF
     primer_bind     4266..4292
                     /label=Q5_010
     primer_bind     complement(4292..4319)
                     /label=Q5_007
     primer_bind     4315..4334
                     /label=455_GL315_KLD_fw
     misc_feature    4321..4329
                     /label=RBS
     CDS             4335..5102
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     complement(4392..4409)
                     /label=GvpF_rv1
     primer_bind     4568..4595
                     /label=Bm3seqF
     primer_bind     5061..5119
                     /label=ESq5mut005
     primer_bind     complement(5075..5102)
                     /label=ESgibson019
     misc_feature    5092..5100
                     /label=RBS
     CDS             5104..5370
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     complement(5302..5318)
                     /label=GvpG_rv1
     primer_bind     5316..5343
                     /label=Bm4seqF
     primer_bind     5326..5385
                     /label=ESq5mut007
     primer_bind     complement(5338..5371)
                     /label=G dL r
     primer_bind     complement(5338..5371)
                     /label=G dLS r
     misc_feature    5357..5365
                     /label=RBS
     CDS             5371..6180
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    5441
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5510
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5960
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6091..6117
                     /label=Bm5seqF
     primer_bind     6181..6215
                     /label=G dL f
     primer_bind     complement(6181..6204)
                     /label=G dL r
     misc_feature    6182..6190
                     /label=RBS
     CDS             6198..6485
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(6270..6286)
                     /label=GvpS_rv1
     primer_bind     complement(6321..6349)
                     /label=V S-K fix r
     primer_bind     6334..6367
                     /label=F S-K fix f
     RBS             6424..6432
                     /label=RBS for K
     primer_bind     complement(6484..6498)
                     /label=G dLS r
     primer_bind     6486..6505
                     /label=G dLS f
     primer_bind     complement(6519..6553)
                     /label=G dK r
     misc_feature    6520^6521
                     /label=SK
     primer_bind     6539..6558
                     /label=G dK f
     primer_bind     6539..6553
                     /label=V S-K fix f
     misc_feature    6548..6556
                     /label=RBS
     CDS             6560..6862
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    6685
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6691..6712
                     /label=Bm6seqF
     primer_bind     complement(6859..6884)
                     /label=452_GL313_KLD_rv
     primer_bind     complement(6866..6897)
                     /label=G dTU r
     misc_feature    6885..6893
                     /label=RBS
     CDS             6899..7777
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     6920..6946
                     /label=Bm6.1seq
     primer_bind     complement(6956..6974)
                     /label=GvpT_rv1
     misc_feature    7223
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    7286
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     7360..7386
                     /label=Bm7seqF
     primer_bind     complement(7763..7784)
                     /label=454_GL314_KLD_rv
     primer_bind     7778..7798
                     /label=451_GL313_KLD_fw
     misc_feature    7785..7793
                     /label=RBS
     CDS             7801..8217
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     complement(7858..7875)
                     /label=GvpU_rv1
     primer_bind     8029..8044
                     /label=GvpU_fw1
     primer_bind     8140..8164
                     /label=GvpUseqF
     primer_bind     8213..8235
                     /label=G dTU f
     primer_bind     complement(8213..8229)
                     /label=G dTU r
     primer_bind     8218..8235
                     /label=453_GL314_KLD_fw
     primer_bind     8236..8250
                     /label=G dTU r
     primer_bind     8362..8385
                     /label=390_A1serr_bb1_fw_4-frag
     primer_bind     8370..8395
                     /label=353_AC_backbone1_fw
     primer_bind     complement(8372..8395)
                     /label=393_A1serr_bb3_rv_4-frag
     primer_bind     8424..8444
                     /label=418_gib_GL302_fw
     primer_bind     complement(8424..8443)
                     /label=Gibson022
     primer_bind     8424..8442
                     /label=422_gib_bck_fw
     primer_bind     8424..8442
                     /label=Gibson020
     primer_bind     8424..8442
                     /label=Q5_002
     primer_bind     8424..8442
                     /label=Q5_006
     primer_bind     8424..8442
                     /label=Q5mut011
     primer_bind     8424..8442
                     /label=Q5mut011 (1)
     primer_bind     complement(8430..8451)
                     /label=354_AC_B_backbone2_rv
     primer_bind     complement(8471..8490)
                     /label=T7 Terminator
     primer_bind     complement(8472..8490)
                     /label=Eurofins_T7term
     terminator      8487..8534
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
       61 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      121 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      181 tcacccagaa acgctcgtga aagtaaaaga cgcagaggac caattggggg cacgagtggg
      241 atacatagaa ctggacttga atagcggtaa aatccttgag agttttcgcc ctgaagagcg
      301 ttttccaatg atgagcactt tcaaagttct gctatgtgga gcagtattat cccgtgtaga
      361 tgcggggcaa gagcaactcg gacgacgaat acactattcg cagaatgact tggttgaata
      421 ctccccagtg acagaaaagc accttacgga cggaatgacg gtaagagaat tatgtagtgc
      481 cgccataacg atgagtgata acactgcggc gaacttactt ctgacaacca tcggtggacc
      541 gaaggaatta accgcttttt tgcacaatat gggagaccat gtaactcgcc ttgaccgttg
      601 ggaaccagaa ctgaatgaag ccataccaaa cgacgagcga gacaccacaa tgcctgcggc
      661 aatggcaaca acattacgca aactattaac tggcgaacta cttactctgg cttcacggca
      721 acaattaata gactggcttg aagcggataa agttgcagga ccactactgc gttcggcact
      781 tcctgctggc tggtttattg ctgataaatc tggggcagga gagcgtggtt cacggggtat
      841 cattgccgca cttggaccag atggtaagcc ttcccgtatc gtagttatct acacgacggg
      901 tagtcaggca actatggacg aacgaaatag acagattgct gaaatagggg cttcactgat
      961 taagcattgg taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgga
     1021 ccggtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     1201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1621 cggagcctat ggaaaaacgc cagcaacgcg gcccttttta cggttcctgg ccttttgctg
     1681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1741 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1801 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1861 ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     1921 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     1981 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     2041 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     2101 agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
     2161 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
     2221 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
     2281 tgttcatggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     2341 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     2401 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     2461 tgttggcacc atgcatcgat cccgcgaaat taatacgact cactataggg agaccacaac
     2521 ggtttccctc tagagatagc ttagcgatgt gaaagaagct ggagaaaact ttcaagaaaa
     2581 agtttcttct tcagacgata aagagaagtc aaaaaataag cggaaaataa aaggcgtaaa
     2641 tcagattaaa aaagcaacgg atattaaaag ctctactaag attaaaagtt ctaatgatat
     2701 taaatcatct acagatttaa aaacaatggg atcataagcg aaaggagaaa tgagatatgt
     2761 ctattcaaaa aagtactaat agttcaagtt tagcagaagt cattgaccgt attttagata
     2821 aaggaattgt tattgatgct tttgcaagag tttctgttgt aggaattgaa attttaacga
     2881 ttgaagcgcg agtggttatt gccagtgttg atacatggtt acgctatgca gaagcagtag
     2941 ggcttcttcg tgacgacgta gaagaaaacg gtcttcctga acgttcaaat tcaagtgaag
     3001 ggcagccgcg ttttagtatt taatatgaag tggtatggaa ccttcctttt ttgagggtgg
     3061 ttccagcttt tagatctaac tattggaggc tactaaaaat ggaaattaaa aaaattatgc
     3121 aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt acctcggtgg
     3181 aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa cgagaatata
     3241 tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta aataaagaaa
     3301 aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt ggcattgaag
     3361 cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga caaaaggaaa
     3421 aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc aagagcgctg
     3481 agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg aggcaaaacc
     3541 tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat gcacgggaat
     3601 cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag caaaaaagta
     3661 atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga aaactggcag
     3721 gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga cgaatttact
     3781 cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg cgtgctgccg
     3841 ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt ccgcgtcatc
     3901 ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc gcttctcgac
     3961 aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc gattttaacg
     4021 gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt agcaaacgtg
     4081 cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc gcttatgatc
     4141 gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga ttttcaaacg
     4201 ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga aaatgcaaaa
     4261 agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga agaaaagtaa
     4321 aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt agcgccattc
     4381 aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa gctgaaacat
     4441 ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg aaaatttatc
     4501 atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt atggacaaga
     4561 acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa gacgtaaaag
     4621 ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc aaaggaaaaa
     4681 ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa gtaaacgaaa
     4741 atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca gccggttatt
     4801 atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg caaaaagaag
     4861 tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa gcaaatgagc
     4921 caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa gatgaagcga
     4981 agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc gattttcatt
     5041 acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta gaagagaaat
     5101 aacgtgcttc acaaattagt aaccgcaccc attaaccttg tagtgaaaat cggcgaaaaa
     5161 gtacaggaag aagctgataa acagctatat gaccttccga cgattcagca aaagctcatt
     5221 cagcttcaaa tgatgtttga gcttggtgaa attccagaag aagcgtttca agaaaaagaa
     5281 gatgaattgt taatgaggta cgaaattgcg aaacgcagag aaattgaaca atgggaagag
     5341 ctaacacaaa aaagaaatga ggaatcctag atgggagaat tactgtattt atacggttta
     5401 attccaacaa aagaagcagc agccatagag ccgtttccat cttataaggg gtttgacgga
     5461 gaacattcac tgtacccaat tgcgtttgat caggtgacgg ctgtagtttc taagctggat
     5521 gctgacacct attcagaaaa agtgattcaa gaaaaaatgg agcaggatat gagctggctg
     5581 caggaaaaag catttcatca tcacgaaacg gtagccgctt tgtacgaaga atttacgatc
     5641 attccattaa aattttgcac catttataaa ggtgaagaaa gtctgcaggc agctattgag
     5701 attaacaaag aaaagataga gaattcactg acgctgcttc aaggaaatga agagtggaat
     5761 gtgaaaattt actgtgatga tacagagctt aaaaaaggaa tcagcgaaac gaatgaaagc
     5821 gtgaaagcga aaaaacaaga aattagtcac ttatcaccag gaagacagtt ttttgaaaag
     5881 aaaaaaatag atcagctgat tgaaaaagaa ttagagcttc acaaaaacaa agtgtgtgaa
     5941 gagatacatg acaagctaaa agaattatcg ctttatgact ctgttaaaaa gaattggagc
     6001 aaagacgtaa ctggcgcagc tgaacagatg gcgtggaaca gcgtgtttct tctcccgtct
     6061 ctgcagatta ctaagttcgt aaacgaaata gaagagcttc agcaaaggct tgaaaataaa
     6121 ggctggaagt ttgaagtgac gggaccatgg ccgccctatc atttctcgag ctttgcgtaa
     6181 agtgaggaat taacattatg tctcttaaac aatccatgga gaataaagat attgctctta
     6241 ttgatatttt agatgtcatt ttagataaag gagtcgccat taaaggagac ttaatcattt
     6301 ccatagctgg cgtcgattta gtgtatttgg atttgcgggt gcttatttct tcggttgaaa
     6361 cgcttgtgca agcaaaagaa ggaaatcaca aaccaatcac ttctgaacaa tttgataaac
     6421 aaaaagaaga gctgatggac gctacgggcc agcctagtaa gtggacgaac cctctaggtt
     6481 cgtgaccctt gcgagcagtc gtatgctttc tcgaattccg agcggttaag cgtgacaggc
     6541 ggtcagtagg aggaacagta tggcagtcga acataatatg cagtcaagta cgattgtaga
     6601 tgtgctcgaa aagattttgg ataaaggagt cgttatagcg ggggacatca ccgtaggaat
     6661 tgcagatgtc gagctattaa cgataaagat ccgcttgatt gtggcttcgg ttgataaggc
     6721 aaaagaaatc ggcatggact ggtgggaaaa tgatccgtat ctcagttcaa aaggagccaa
     6781 taacaaagcg ctcgaagaag aaaataaaat gctgcatgag cggttaaaaa cgcttgaaga
     6841 aaaaatagaa acgaaacgtt aaaaactgta cgctacttaa aaaatggagg gatttacaat
     6901 ggcaactgaa acaaaattag ataacacaca ggcagaaaac aaggaaaata aaaatgcgga
     6961 aaacggttca aaagaaaaga acggttcaaa agcaagcaaa acaacaagca gcgggccaat
     7021 caaacgagcg gtagcaggag gcatcatcgg tgcaacgatt ggatatgtat cgactcctga
     7081 aaatcgaaaa agtctccttg accgcattga tacagacgaa ttaaaaagca aagcatctga
     7141 tttaggaaca aaggtaaaag aaaaatcaaa aagcagcgtg gccagcctga aaacatctgc
     7201 gggaagcttg tttaaaaaag ataaagataa atcaaaagat gatgaagaaa acgtaaattc
     7261 ttctagtagc gaaacagaag acgataacgt tcaagagtac gacgagttaa aagaagaaaa
     7321 tcaaactctt caagatcgct tatcacagct tgaagaaaaa atgaacatgc ttgttgagct
     7381 tagcctcaat aaaaatcaag acgaagaagc ggaagataca gattccgacg aagaagagaa
     7441 cgatgagaac gatgaaaacg atgaaaacga gcaggacgat gaaaacgaag aagaaacatc
     7501 taagccacgt aaaaaggata aaaaagaagc tgaggaagaa gaaagtgaaa gtgacgaaga
     7561 cagcgaggaa gaagaggaag attctcgctc aaacaaaaaa aataaaaaag taaaaacaga
     7621 agaagaagac gaagatgaaa gcgaagaaga aaaaaaggaa gcgaaaccaa aaaagtcaac
     7681 agctaaaaaa tcaaaaaata caaaagcaaa gaaaaacacg gacgaagaag atgatgaagc
     7741 aacatctctt tctagtgaag acgatacaac agcctaagac gtaaaggagg aaagaaagac
     7801 atgagtacag gcccttcttt ttcaactaaa gacaatacgc ttgaatactt tgtgaaagct
     7861 tctaataaac acggcttttc acttgatatt tcattaaatg taaacggcgc tgtgatttcc
     7921 ggtaccatga tttcagcaaa agaatacttt gattacttaa gcgaaacgtt tgaagaaggc
     7981 agtgaagtgg ctcaggcgct aagcgaacaa ttctctttag caagcgaagc gagcgaatca
     8041 aacggagaag cagaagccca ttttattcat ttgaaaaata caaagattta ctgtggagac
     8101 agtaaatcta ctccttctaa aggcaaaatc ttttggagag ggaaaatagc agaagtagac
     8161 gggtttttct taggaaagat ttctgatgca aaatcaacga gtaaaaagag ttcataaaaa
     8221 acggcggggt gattgccccg ccgtttttta gtgatgtgat gagatgtgca gcttcttttt
     8281 tcgatacatg agcggagtca tcttcatttc ttttcgaaac tgattaataa aatagcttgt
     8341 actattaaac cctacttgat aggcgacttc cgtcacattt gcttctgctt gctgcagtcc
     8401 ggattcgcga acgcgtctcg agagatccgg ctgctaacaa agcccgaaag gaagctgagt
     8461 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     8521 tgaggggttt tttgctgaaa ggaggaacta tatccgacta gtagcccgcc taatgagcgg
//
