LOCUS       Exported                8564 bp ds-DNA     circular SYN 10-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        29..120
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(49..69)
                     /label=AmpR_rv1
     RBS             110..115
                     /label=Emopec_63.3%
     CDS             121..981
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1055..1643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1576..1591
                     /label=ori_fw1
     primer_bind     1744..1763
                     /label=Eurofins_VR
     misc_feature    1830..1969
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2001..2021)
                     /label=Eurofins_pexF
     primer_bind     2055..2077
                     /label=pGEX 3'
     primer_bind     2059..2077
                     /label=Eurofins_Gex-Reverse (formerly pGexR)
     CDS             complement(2071..2262)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2499..2518
                     /label=T7 Promoter
     promoter        2499..2517
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2715..2737
                     /label=414_gibson_gvpB_fw
     primer_bind     complement(2736..2760)
                     /label=368_B_backbone1_rv_tile1
     primer_bind     2736..2760
                     /label=372_B_tile1_fw
     primer_bind     complement(2736..2760)
                     /label=404_mega_bb2_rv
     misc_feature    2751..2759
                     /label=RBS
     CDS             2765..3031
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     misc_feature    2840..2866
                     /label=beta-strand1
     primer_bind     complement(2867..2902)
                     /label=369_B_backbone1_rv_tile2
     primer_bind     2867..2896
                     /label=374_B_tile2_fw
     primer_bind     2903..2935
                     /label=370_B_backbone2_fw_tile1
     primer_bind     complement(2910..2935)
                     /label=373_B_tile1_rv
     primer_bind     complement(2938..2955)
                     /label=399_B_V68C_KLD_rv_v2
     primer_bind     complement(2940..2957)
                     /label=395_B_V68C_KLD_rv
     primer_bind     2969..2991
                     /label=401_mega_V68C_G82L_bb1_fw
     primer_bind     3016..3050
                     /label=400_mega_V68C_only_bb1_fw
     primer_bind     complement(3017..3041)
                     /label=415_gibson_gvpB_rv
     primer_bind     3038..3063
                     /label=371_B_backbone2_fw_tile2
     primer_bind     complement(3041..3066)
                     /label=375_B_tile2_rv
     primer_bind     3054..3079
                     /label=Bm1seq
     primer_bind     complement(3071..3091)
                     /label=450_GL312_KLD_rv
     misc_feature    3092..3100
                     /label=RBS
     CDS             3107..3373
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(3206..3221)
                     /label=352_gvpR_rv1
     primer_bind     3332..3352
                     /label=Q5_008
     primer_bind     complement(3369..3386)
                     /label=456_GL315_KLD_rv
     primer_bind     3374..3391
                     /label=449_GL312_KLD_fw
     misc_feature    3387..3395
                     /label=RBS
     CDS             3402..4328
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     3432..3457
                     /label=Bm1.1seqF
     misc_feature    3464
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     complement(3502..3517)
                     /label=GvpN_rv1
     primer_bind     3834..3853
                     /label=Bm2seqF
     primer_bind     4274..4300
                     /label=Q5_010
     primer_bind     complement(4300..4327)
                     /label=Q5_007
     primer_bind     4323..4342
                     /label=455_GL315_KLD_fw
     misc_feature    4329..4337
                     /label=RBS
     CDS             4343..5110
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     complement(4400..4417)
                     /label=GvpF_rv1
     primer_bind     4576..4603
                     /label=Bm3seqF
     primer_bind     5069..5127
                     /label=ESq5mut005
     primer_bind     complement(5078..5112)
                     /label=G dG r
     primer_bind     complement(5083..5110)
                     /label=ESgibson019
     misc_feature    5100..5108
                     /label=RBS
     CDS             5112..5378
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     complement(5310..5326)
                     /label=GvpG_rv1
     primer_bind     5324..5351
                     /label=Bm4seqF
     primer_bind     5334..5393
                     /label=ESq5mut007
     primer_bind     complement(5346..5379)
                     /label=G dL r
     primer_bind     complement(5346..5379)
                     /label=G dLS r
     primer_bind     5362..5402
                     /label=G dG f
     primer_bind     complement(5362..5382)
                     /label=G dG r
     misc_feature    5365..5373
                     /label=RBS
     CDS             5379..6188
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    5449
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5518
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5968
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6099..6125
                     /label=Bm5seqF
     primer_bind     6189..6223
                     /label=G dL f
     primer_bind     complement(6189..6212)
                     /label=G dL r
     misc_feature    6190..6198
                     /label=RBS
     CDS             6206..6493
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(6278..6294)
                     /label=GvpS_rv1
     primer_bind     complement(6329..6357)
                     /label=V S-K fix r
     primer_bind     6342..6375
                     /label=F S-K fix f
     RBS             6432..6440
                     /label=RBS for K
     primer_bind     complement(6492..6506)
                     /label=G dLS r
     primer_bind     6494..6513
                     /label=G dLS f
     primer_bind     complement(6527..6546)
                     /label=G dK r
     misc_feature    6528^6529
                     /label=SK
     misc_feature    6550..6558
                     /label=RBS
     CDS             6565..6849
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(6829..6857)
                     /label=F S-K fix r
     primer_bind     complement(6837..6879)
                     /label=G dJ r
     primer_bind     6842..6857
                     /label=V S-K fix f
     primer_bind     complement(6858..6889)
                     /label=G dTU r
     primer_bind     6858..6889
                     /label=G dJ f
     primer_bind     complement(6858..6876)
                     /label=452_GL313_KLD_rv
     misc_feature    6877..6885
                     /label=RBS
     CDS             6891..7769
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     6912..6938
                     /label=Bm6.1seq
     primer_bind     complement(6948..6966)
                     /label=GvpT_rv1
     misc_feature    7215
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    7278
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     7352..7378
                     /label=Bm7seqF
     primer_bind     complement(7755..7776)
                     /label=454_GL314_KLD_rv
     primer_bind     7770..7790
                     /label=451_GL313_KLD_fw
     misc_feature    7777..7785
                     /label=RBS
     CDS             7793..8209
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     complement(7850..7867)
                     /label=GvpU_rv1
     primer_bind     8021..8036
                     /label=GvpU_fw1
     primer_bind     8132..8156
                     /label=GvpUseqF
     primer_bind     8205..8227
                     /label=G dTU f
     primer_bind     complement(8205..8221)
                     /label=G dTU r
     primer_bind     8210..8227
                     /label=453_GL314_KLD_fw
     primer_bind     8228..8242
                     /label=G dTU r
     primer_bind     8354..8377
                     /label=390_A1serr_bb1_fw_4-frag
     primer_bind     8362..8387
                     /label=353_AC_backbone1_fw
     primer_bind     complement(8364..8387)
                     /label=393_A1serr_bb3_rv_4-frag
     primer_bind     8416..8436
                     /label=418_gib_GL302_fw
     primer_bind     complement(8416..8435)
                     /label=Gibson022
     primer_bind     8416..8434
                     /label=422_gib_bck_fw
     primer_bind     8416..8434
                     /label=Gibson020
     primer_bind     8416..8434
                     /label=Q5_002
     primer_bind     8416..8434
                     /label=Q5_006
     primer_bind     8416..8434
                     /label=Q5mut011
     primer_bind     8416..8434
                     /label=Q5mut011 (1)
     primer_bind     complement(8422..8443)
                     /label=354_AC_B_backbone2_rv
     primer_bind     complement(8463..8482)
                     /label=T7 Terminator
     primer_bind     complement(8464..8482)
                     /label=Eurofins_T7term
     terminator      8479..8526
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
       61 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      121 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      181 gtttttgctc acccagaaac gctcgtgaaa gtaaaagacg cagaggacca attgggggca
      241 cgagtgggat acatagaact ggacttgaat agcggtaaaa tccttgagag ttttcgccct
      301 gaagagcgtt ttccaatgat gagcactttc aaagttctgc tatgtggagc agtattatcc
      361 cgtgtagatg cggggcaaga gcaactcgga cgacgaatac actattcgca gaatgacttg
      421 gttgaatact ccccagtgac agaaaagcac cttacggacg gaatgacggt aagagaatta
      481 tgtagtgccg ccataacgat gagtgataac actgcggcga acttacttct gacaaccatc
      541 ggtggaccga aggaattaac cgcttttttg cacaatatgg gagaccatgt aactcgcctt
      601 gaccgttggg aaccagaact gaatgaagcc ataccaaacg acgagcgaga caccacaatg
      661 cctgcggcaa tggcaacaac attacgcaaa ctattaactg gcgaactact tactctggct
      721 tcacggcaac aattaataga ctggcttgaa gcggataaag ttgcaggacc actactgcgt
      781 tcggcacttc ctgctggctg gtttattgct gataaatctg gggcaggaga gcgtggttca
      841 cggggtatca ttgccgcact tggaccagat ggtaagcctt cccgtatcgt agttatctac
      901 acgacgggta gtcaggcaac tatggacgaa cgaaatagac agattgctga aataggggct
      961 tcactgatta agcattggta aaccgataca attaaaggct ccttttggag cctttttttt
     1021 tggacggacc ggtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1201 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc cctttttacg gttcctggcc
     1681 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1741 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1801 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     1861 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     1921 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     1981 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2041 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2101 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     2161 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     2221 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     2281 gggatttctg ttcatggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     2341 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     2401 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     2461 gatgtaggtg ttggcaccat gcatcgatcc cgcgaaatta atacgactca ctatagggag
     2521 accacaacgg tttccctcta gagatagctt agcgatgtga aagaagctgg agaaaacttt
     2581 caagaaaaag tttcttcttc agacgataaa gagaagtcaa aaaataagcg gaaaataaaa
     2641 ggcgtaaatc agattaaaaa agcaacggat attaaaagct ctactaagat taaaagttct
     2701 aatgatatta aatcatctac agatttaaaa acaatgggat cataagcgaa aggagaaatg
     2761 agatatgtct attcaaaaaa gtactaatag ttcaagttta gcagaagtca ttgaccgtat
     2821 tttagataaa ggaattgtta ttgatgcttt tgcaagagtt tctgttgtag gaattgaaat
     2881 tttaacgatt gaagcgcgag tggttattgc cagtgttgat acatggttac gctatgcaga
     2941 agcagtaggg cttcttcgtg acgacgtaga agaaaacggt cttcctgaac gttcaaattc
     3001 aagtgaaggg cagccgcgtt ttagtattta atatgaagtg gtatggaacc ttcctttttt
     3061 gagggtggtt ccagctttta gatctaacta ttggaggcta ctaaaaatgg aaattaaaaa
     3121 aattatgcaa gccgtgaacg actttttcgg tgaacacgta gctcctcctc ataaaattac
     3181 ctcggtggaa gctactgaag atgaaggttg gagagttatt gttgaagtca ttgaagaacg
     3241 agaatatatg aaaaaatacg ccaaagatga aatgctcgga acgtacgagt gctttgtaaa
     3301 taaagaaaaa gaagtcattt cattcaaacg actcgacgtc agatatagaa gcgccattgg
     3361 cattgaagca taaacgataa gatggcagga ggaacgtaaa aatgaccgtc ttaacagaca
     3421 aaaggaaaaa aggcagtgga gcttttatac aagatgacga gacaaaagag gttctttcaa
     3481 gagcgctgag ctatttaaaa tccggctatt ccattcattt tacaggtcct gccggcggag
     3541 gcaaaacctc tttagcgcga gcgcttgcta aaaagagaaa gcgtcctgta atgctgatgc
     3601 acgggaatca cgagctcaac aacaaagatt taattggcga ttttacggga tacacgagca
     3661 aaaaagtaat cgaccagtac gttcgttctg tctataaaaa agatgaacag gtgagtgaaa
     3721 actggcagga tggccgattg cttgaagctg taaaaaatgg ctatacgctg atttacgacg
     3781 aatttactcg ttctaagcct gcgacgaata atatctttct atcgatatta gaagaaggcg
     3841 tgctgccgct gtatggagta aaaatgaccg atccttttgt gcgcgtgcat cccgatttcc
     3901 gcgtcatctt cacaagcaat ccagctgagt atgccggcgt atatgatacg caagatgcgc
     3961 ttctcgacag gttaattacc atgtttattg attataaaga catcgacaga gagacagcga
     4021 ttttaacgga gaaaacggac gtagaagaag atgaagcgcg cacaattgta acgctcgtag
     4081 caaacgtgcg aaaccgctct ggagacgaaa acagcagcgg acttagcctg cgggcttcgc
     4141 ttatgatcgc tacccttgcc acgcagcaag acattcctat cgatggaagt gacgaagatt
     4201 ttcaaacgtt atgtatcgat attttgcatc atccgcttac caaatgtttg gatgaagaaa
     4261 atgcaaaaag caaagccgaa aaaatcattt tagaagaatg taagaatata gacactgaag
     4321 aaaagtaaag gagcttgaaa acatgagtga aacaaacgaa acaggtattt atatttttag
     4381 cgccattcaa acggataaag acgaagaatt tggcgccgtg gaagtagaag gaacaaaagc
     4441 tgaaacattt ttgattcgct acaaagacgc ggctatggta gcagctgaag taccgatgaa
     4501 aatttatcat cctaatcgcc aaaatttatt aatgcatcaa aacgcagtag cagcgattat
     4561 ggacaagaac gatacggtta ttccaatcag ctttgggaat gtattcaaat caaaagaaga
     4621 cgtaaaagtt cttttggaaa acctttatcc gcagtttgaa aagctgtttc cagcgatcaa
     4681 aggaaaaatt gaagtcggtt taaaagtaat tgggaaaaaa gaatggcttg agaaaaaagt
     4741 aaacgaaaat cctgaacttg agaaagtatc agcatccgta aaaggaaaat cagaagcagc
     4801 cggttattat gagcgtattc aacttggagg aatggctcaa aagatgttta cttccctgca
     4861 aaaagaagtc aagacagatg tgttttctcc gcttgaagaa gcagcggaag cagcaaaagc
     4921 aaatgagcca acgggcgaaa cgatgctttt aaacgcgtct ttcttaatta accgagaaga
     4981 tgaagcgaag tttgatgaaa aagtaaatga agcgcatgaa aactggaaag acaaagccga
     5041 ttttcattac agcggtcctt ggcctgctta taattttgtg aacattcgcc taaaagtaga
     5101 agagaaataa cgtgcttcac aaattagtaa ccgcacccat taaccttgta gtgaaaatcg
     5161 gcgaaaaagt acaggaagaa gctgataaac agctatatga ccttccgacg attcagcaaa
     5221 agctcattca gcttcaaatg atgtttgagc ttggtgaaat tccagaagaa gcgtttcaag
     5281 aaaaagaaga tgaattgtta atgaggtacg aaattgcgaa acgcagagaa attgaacaat
     5341 gggaagagct aacacaaaaa agaaatgagg aatcctagat gggagaatta ctgtatttat
     5401 acggtttaat tccaacaaaa gaagcagcag ccatagagcc gtttccatct tataaggggt
     5461 ttgacggaga acattcactg tacccaattg cgtttgatca ggtgacggct gtagtttcta
     5521 agctggatgc tgacacctat tcagaaaaag tgattcaaga aaaaatggag caggatatga
     5581 gctggctgca ggaaaaagca tttcatcatc acgaaacggt agccgctttg tacgaagaat
     5641 ttacgatcat tccattaaaa ttttgcacca tttataaagg tgaagaaagt ctgcaggcag
     5701 ctattgagat taacaaagaa aagatagaga attcactgac gctgcttcaa ggaaatgaag
     5761 agtggaatgt gaaaatttac tgtgatgata cagagcttaa aaaaggaatc agcgaaacga
     5821 atgaaagcgt gaaagcgaaa aaacaagaaa ttagtcactt atcaccagga agacagtttt
     5881 ttgaaaagaa aaaaatagat cagctgattg aaaaagaatt agagcttcac aaaaacaaag
     5941 tgtgtgaaga gatacatgac aagctaaaag aattatcgct ttatgactct gttaaaaaga
     6001 attggagcaa agacgtaact ggcgcagctg aacagatggc gtggaacagc gtgtttcttc
     6061 tcccgtctct gcagattact aagttcgtaa acgaaataga agagcttcag caaaggcttg
     6121 aaaataaagg ctggaagttt gaagtgacgg gaccatggcc gccctatcat ttctcgagct
     6181 ttgcgtaaag tgaggaatta acattatgtc tcttaaacaa tccatggaga ataaagatat
     6241 tgctcttatt gatattttag atgtcatttt agataaagga gtcgccatta aaggagactt
     6301 aatcatttcc atagctggcg tcgatttagt gtatttggat ttgcgggtgc ttatttcttc
     6361 ggttgaaacg cttgtgcaag caaaagaagg aaatcacaaa ccaatcactt ctgaacaatt
     6421 tgataaacaa aaagaagagc tgatggacgc tacgggccag cctagtaagt ggacgaaccc
     6481 tctaggttcg tgacccttgc gagcagtcgt atgctttctc gaattccgag cggttaagcg
     6541 tgacagtcca ggaggaatta atagatgcaa ccggtcagcc aagcaaatgg acgaatccac
     6601 ttggatcctg atcaagctga acaaggctta gcgcagcttg tgatgacagt tattgagcta
     6661 ttgaggcaaa tcgttgaacg tcatgccatg aggcgggtgg agggtggaac gttgacggac
     6721 gaacaaattg aaaacttagg aattgcacta atgaacttag aagaaaaaat ggacgagttg
     6781 aaagaggtgt tcggtctgga tgcagaagat ttaaatattg atcttggccc gctaggcagc
     6841 ctgctttaag cggtcagctg tacgctactt aaaaaatgga gggatttaca atggcaactg
     6901 aaacaaaatt agataacaca caggcagaaa acaaggaaaa taaaaatgcg gaaaacggtt
     6961 caaaagaaaa gaacggttca aaagcaagca aaacaacaag cagcgggcca atcaaacgag
     7021 cggtagcagg aggcatcatc ggtgcaacga ttggatatgt atcgactcct gaaaatcgaa
     7081 aaagtctcct tgaccgcatt gatacagacg aattaaaaag caaagcatct gatttaggaa
     7141 caaaggtaaa agaaaaatca aaaagcagcg tggccagcct gaaaacatct gcgggaagct
     7201 tgtttaaaaa agataaagat aaatcaaaag atgatgaaga aaacgtaaat tcttctagta
     7261 gcgaaacaga agacgataac gttcaagagt acgacgagtt aaaagaagaa aatcaaactc
     7321 ttcaagatcg cttatcacag cttgaagaaa aaatgaacat gcttgttgag cttagcctca
     7381 ataaaaatca agacgaagaa gcggaagata cagattccga cgaagaagag aacgatgaga
     7441 acgatgaaaa cgatgaaaac gagcaggacg atgaaaacga agaagaaaca tctaagccac
     7501 gtaaaaagga taaaaaagaa gctgaggaag aagaaagtga aagtgacgaa gacagcgagg
     7561 aagaagagga agattctcgc tcaaacaaaa aaaataaaaa agtaaaaaca gaagaagaag
     7621 acgaagatga aagcgaagaa gaaaaaaagg aagcgaaacc aaaaaagtca acagctaaaa
     7681 aatcaaaaaa tacaaaagca aagaaaaaca cggacgaaga agatgatgaa gcaacatctc
     7741 tttctagtga agacgataca acagcctaag acgtaaagga ggaaagaaag acatgagtac
     7801 aggcccttct ttttcaacta aagacaatac gcttgaatac tttgtgaaag cttctaataa
     7861 acacggcttt tcacttgata tttcattaaa tgtaaacggc gctgtgattt ccggtaccat
     7921 gatttcagca aaagaatact ttgattactt aagcgaaacg tttgaagaag gcagtgaagt
     7981 ggctcaggcg ctaagcgaac aattctcttt agcaagcgaa gcgagcgaat caaacggaga
     8041 agcagaagcc cattttattc atttgaaaaa tacaaagatt tactgtggag acagtaaatc
     8101 tactccttct aaaggcaaaa tcttttggag agggaaaata gcagaagtag acgggttttt
     8161 cttaggaaag atttctgatg caaaatcaac gagtaaaaag agttcataaa aaacggcggg
     8221 gtgattgccc cgccgttttt tagtgatgtg atgagatgtg cagcttcttt tttcgataca
     8281 tgagcggagt catcttcatt tcttttcgaa actgattaat aaaatagctt gtactattaa
     8341 accctacttg ataggcgact tccgtcacat ttgcttctgc ttgctgcagt ccggattcgc
     8401 gaacgcgtct cgagagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     8461 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     8521 tttttgctga aaggaggaac tatatccgac tagtagcccg ccta
//
