LOCUS       Exported                8471 bp ds-DNA     circular SYN 10-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        30..121
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(50..70)
                     /label=AmpR_rv1
     RBS             111..116
                     /label=Emopec_63.3%
     CDS             122..982
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1056..1644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1577..1592
                     /label=ori_fw1
     primer_bind     1745..1764
                     /label=Eurofins_VR
     misc_feature    1831..1970
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2002..2022)
                     /label=Eurofins_pexF
     primer_bind     2056..2078
                     /label=pGEX 3'
     primer_bind     2060..2078
                     /label=Eurofins_Gex-Reverse (formerly pGexR)
     CDS             complement(2072..2263)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2500..2519
                     /label=T7 Promoter
     promoter        2500..2518
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2716..2738
                     /label=414_gibson_gvpB_fw
     primer_bind     complement(2737..2761)
                     /label=368_B_backbone1_rv_tile1
     primer_bind     2737..2761
                     /label=372_B_tile1_fw
     primer_bind     complement(2737..2761)
                     /label=404_mega_bb2_rv
     misc_feature    2752..2760
                     /label=RBS
     CDS             2766..3032
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     misc_feature    2841..2867
                     /label=beta-strand1
     primer_bind     complement(2868..2903)
                     /label=369_B_backbone1_rv_tile2
     primer_bind     2868..2897
                     /label=374_B_tile2_fw
     primer_bind     2904..2936
                     /label=370_B_backbone2_fw_tile1
     primer_bind     complement(2911..2936)
                     /label=373_B_tile1_rv
     primer_bind     complement(2939..2956)
                     /label=399_B_V68C_KLD_rv_v2
     primer_bind     complement(2941..2958)
                     /label=395_B_V68C_KLD_rv
     primer_bind     2970..2992
                     /label=401_mega_V68C_G82L_bb1_fw
     primer_bind     3017..3051
                     /label=400_mega_V68C_only_bb1_fw
     primer_bind     complement(3018..3042)
                     /label=415_gibson_gvpB_rv
     primer_bind     3039..3064
                     /label=371_B_backbone2_fw_tile2
     primer_bind     complement(3042..3067)
                     /label=375_B_tile2_rv
     primer_bind     3055..3080
                     /label=Bm1seq
     primer_bind     complement(3072..3092)
                     /label=450_GL312_KLD_rv
     misc_feature    3093..3101
                     /label=RBS
     CDS             3108..3374
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(3207..3222)
                     /label=352_gvpR_rv1
     primer_bind     3333..3353
                     /label=Q5_008
     primer_bind     complement(3360..3387)
                     /label=G dN r
     primer_bind     complement(3370..3387)
                     /label=456_GL315_KLD_rv
     primer_bind     3375..3392
                     /label=449_GL312_KLD_fw
     misc_feature    3388..3396
                     /label=RBS
     CDS             3403..4329
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     3433..3458
                     /label=Bm1.1seqF
     misc_feature    3465
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     complement(3503..3518)
                     /label=GvpN_rv1
     primer_bind     3835..3854
                     /label=Bm2seqF
     primer_bind     4275..4301
                     /label=Q5_010
     primer_bind     complement(4301..4328)
                     /label=Q5_007
     primer_bind     4324..4343
                     /label=455_GL315_KLD_fw
     primer_bind     4326..4358
                     /label=G dN f
     primer_bind     complement(4326..4346)
                     /label=G dN r
     misc_feature    4330..4338
                     /label=RBS
     CDS             4344..5111
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     complement(4401..4418)
                     /label=GvpF_rv1
     primer_bind     4577..4604
                     /label=Bm3seqF
     primer_bind     5070..5128
                     /label=ESq5mut005
     primer_bind     complement(5079..5113)
                     /label=G dG r
     primer_bind     complement(5084..5111)
                     /label=ESgibson019
     misc_feature    5101..5109
                     /label=RBS
     CDS             5113..5379
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     complement(5311..5327)
                     /label=GvpG_rv1
     primer_bind     5325..5352
                     /label=Bm4seqF
     primer_bind     5335..5394
                     /label=ESq5mut007
     primer_bind     complement(5347..5380)
                     /label=G dL r
     primer_bind     complement(5347..5380)
                     /label=G dLS r
     primer_bind     5363..5403
                     /label=G dG f
     primer_bind     complement(5363..5383)
                     /label=G dG r
     misc_feature    5366..5374
                     /label=RBS
     CDS             5380..6189
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    5450
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5519
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5969
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     6100..6126
                     /label=Bm5seqF
     primer_bind     6190..6224
                     /label=G dL f
     primer_bind     complement(6190..6213)
                     /label=G dL r
     misc_feature    6191..6199
                     /label=RBS
     CDS             6207..6494
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(6279..6295)
                     /label=GvpS_rv1
     primer_bind     complement(6330..6358)
                     /label=V S-K fix r
     primer_bind     6343..6376
                     /label=F S-K fix f
     RBS             6433..6441
                     /label=RBS for K
     primer_bind     complement(6493..6507)
                     /label=G dLS r
     primer_bind     6495..6514
                     /label=G dLS f
     primer_bind     complement(6528..6547)
                     /label=G dK r
     misc_feature    6529^6530
                     /label=SK
     misc_feature    6551..6559
                     /label=RBS
     CDS             6566..6850
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(6830..6858)
                     /label=F S-K fix r
     primer_bind     complement(6838..6858)
                     /label=G dJ r
     primer_bind     6843..6865
                     /label=V S-K fix f
     primer_bind     6851..6870
                     /label=G dK f
     primer_bind     complement(6851..6865)
                     /label=G dK r
     misc_feature    6860..6868
                     /label=RBS
     CDS             6872..7174
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    6997
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     7003..7024
                     /label=Bm6seqF
     primer_bind     complement(7171..7196)
                     /label=452_GL313_KLD_rv
     primer_bind     complement(7178..7209)
                     /label=G dTU r
     primer_bind     7178..7209
                     /label=G dJ f
     primer_bind     complement(7178..7209)
                     /label=G dT r
     primer_bind     complement(7178..7199)
                     /label=G dJ r
     misc_feature    7197..7205
                     /label=RBS
     CDS             7211..8089
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     7232..7258
                     /label=Bm6.1seq
     primer_bind     complement(7268..7286)
                     /label=GvpT_rv1
     misc_feature    7535
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    7598
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     7672..7698
                     /label=Bm7seqF
     primer_bind     complement(8075..8096)
                     /label=454_GL314_KLD_rv
     primer_bind     complement(8084..8128)
                     /label=G dU r
     primer_bind     8090..8112
                     /label=G dT f
     primer_bind     8090..8110
                     /label=451_GL313_KLD_fw
     primer_bind     complement(8090..8110)
                     /label=G dT r
     misc_feature    8097..8105
                     /label=RBS
     primer_bind     complement(8111..8129)
                     /label=G dTU r
     primer_bind     8113..8135
                     /label=G dTU f
     CDS             8113..8117
                     /codon_start=1
                     /label=GvpU
                     /translation=""
     primer_bind     8118..8135
                     /label=453_GL314_KLD_fw
     primer_bind     8136..8150
                     /label=G dTU r
     primer_bind     8262..8285
                     /label=390_A1serr_bb1_fw_4-frag
     primer_bind     8270..8295
                     /label=353_AC_backbone1_fw
     primer_bind     complement(8272..8295)
                     /label=393_A1serr_bb3_rv_4-frag
     primer_bind     8324..8344
                     /label=418_gib_GL302_fw
     primer_bind     complement(8324..8343)
                     /label=Gibson022
     primer_bind     8324..8342
                     /label=422_gib_bck_fw
     primer_bind     8324..8342
                     /label=Gibson020
     primer_bind     8324..8342
                     /label=Q5_002
     primer_bind     8324..8342
                     /label=Q5_006
     primer_bind     8324..8342
                     /label=Q5mut011
     primer_bind     8324..8342
                     /label=Q5mut011 (1)
     primer_bind     complement(8330..8351)
                     /label=354_AC_B_backbone2_rv
     primer_bind     complement(8371..8390)
                     /label=T7 Terminator
     primer_bind     complement(8372..8390)
                     /label=Eurofins_T7term
     terminator      8387..8434
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatgagcggg ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat
       61 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      181 tgtttttgct cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc aattgggggc
      241 acgagtggga tacatagaac tggacttgaa tagcggtaaa atccttgaga gttttcgccc
      301 tgaagagcgt tttccaatga tgagcacttt caaagttctg ctatgtggag cagtattatc
      361 ccgtgtagat gcggggcaag agcaactcgg acgacgaata cactattcgc agaatgactt
      421 ggttgaatac tccccagtga cagaaaagca ccttacggac ggaatgacgg taagagaatt
      481 atgtagtgcc gccataacga tgagtgataa cactgcggcg aacttacttc tgacaaccat
      541 cggtggaccg aaggaattaa ccgctttttt gcacaatatg ggagaccatg taactcgcct
      601 tgaccgttgg gaaccagaac tgaatgaagc cataccaaac gacgagcgag acaccacaat
      661 gcctgcggca atggcaacaa cattacgcaa actattaact ggcgaactac ttactctggc
      721 ttcacggcaa caattaatag actggcttga agcggataaa gttgcaggac cactactgcg
      781 ttcggcactt cctgctggct ggtttattgc tgataaatct ggggcaggag agcgtggttc
      841 acggggtatc attgccgcac ttggaccaga tggtaagcct tcccgtatcg tagttatcta
      901 cacgacgggt agtcaggcaa ctatggacga acgaaataga cagattgctg aaataggggc
      961 ttcactgatt aagcattggt aaaccgatac aattaaaggc tccttttgga gccttttttt
     1021 ttggacggac cggtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1081 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1141 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1201 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1261 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1321 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1381 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1441 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1501 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1561 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1621 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg ccctttttac ggttcctggc
     1681 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1741 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     1801 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     1861 gtgcggtatt tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1921 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     1981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     2041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     2101 gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg
     2161 cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga
     2221 taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag
     2281 ggggatttct gttcatgggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     2341 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     2401 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     2461 agatgtaggt gttggcacca tgcatcgatc ccgcgaaatt aatacgactc actataggga
     2521 gaccacaacg gtttccctct agagatagct tagcgatgtg aaagaagctg gagaaaactt
     2581 tcaagaaaaa gtttcttctt cagacgataa agagaagtca aaaaataagc ggaaaataaa
     2641 aggcgtaaat cagattaaaa aagcaacgga tattaaaagc tctactaaga ttaaaagttc
     2701 taatgatatt aaatcatcta cagatttaaa aacaatggga tcataagcga aaggagaaat
     2761 gagatatgtc tattcaaaaa agtactaata gttcaagttt agcagaagtc attgaccgta
     2821 ttttagataa aggaattgtt attgatgctt ttgcaagagt ttctgttgta ggaattgaaa
     2881 ttttaacgat tgaagcgcga gtggttattg ccagtgttga tacatggtta cgctatgcag
     2941 aagcagtagg gcttcttcgt gacgacgtag aagaaaacgg tcttcctgaa cgttcaaatt
     3001 caagtgaagg gcagccgcgt tttagtattt aatatgaagt ggtatggaac cttccttttt
     3061 tgagggtggt tccagctttt agatctaact attggaggct actaaaaatg gaaattaaaa
     3121 aaattatgca agccgtgaac gactttttcg gtgaacacgt agctcctcct cataaaatta
     3181 cctcggtgga agctactgaa gatgaaggtt ggagagttat tgttgaagtc attgaagaac
     3241 gagaatatat gaaaaaatac gccaaagatg aaatgctcgg aacgtacgag tgctttgtaa
     3301 ataaagaaaa agaagtcatt tcattcaaac gactcgacgt cagatataga agcgccattg
     3361 gcattgaagc ataaacgata agatggcagg aggaacgtaa aaatgaccgt cttaacagac
     3421 aaaaggaaaa aaggcagtgg agcttttata caagatgacg agacaaaaga ggttctttca
     3481 agagcgctga gctatttaaa atccggctat tccattcatt ttacaggtcc tgccggcgga
     3541 ggcaaaacct ctttagcgcg agcgcttgct aaaaagagaa agcgtcctgt aatgctgatg
     3601 cacgggaatc acgagctcaa caacaaagat ttaattggcg attttacggg atacacgagc
     3661 aaaaaagtaa tcgaccagta cgttcgttct gtctataaaa aagatgaaca ggtgagtgaa
     3721 aactggcagg atggccgatt gcttgaagct gtaaaaaatg gctatacgct gatttacgac
     3781 gaatttactc gttctaagcc tgcgacgaat aatatctttc tatcgatatt agaagaaggc
     3841 gtgctgccgc tgtatggagt aaaaatgacc gatccttttg tgcgcgtgca tcccgatttc
     3901 cgcgtcatct tcacaagcaa tccagctgag tatgccggcg tatatgatac gcaagatgcg
     3961 cttctcgaca ggttaattac catgtttatt gattataaag acatcgacag agagacagcg
     4021 attttaacgg agaaaacgga cgtagaagaa gatgaagcgc gcacaattgt aacgctcgta
     4081 gcaaacgtgc gaaaccgctc tggagacgaa aacagcagcg gacttagcct gcgggcttcg
     4141 cttatgatcg ctacccttgc cacgcagcaa gacattccta tcgatggaag tgacgaagat
     4201 tttcaaacgt tatgtatcga tattttgcat catccgctta ccaaatgttt ggatgaagaa
     4261 aatgcaaaaa gcaaagccga aaaaatcatt ttagaagaat gtaagaatat agacactgaa
     4321 gaaaagtaaa ggagcttgaa aacatgagtg aaacaaacga aacaggtatt tatattttta
     4381 gcgccattca aacggataaa gacgaagaat ttggcgccgt ggaagtagaa ggaacaaaag
     4441 ctgaaacatt tttgattcgc tacaaagacg cggctatggt agcagctgaa gtaccgatga
     4501 aaatttatca tcctaatcgc caaaatttat taatgcatca aaacgcagta gcagcgatta
     4561 tggacaagaa cgatacggtt attccaatca gctttgggaa tgtattcaaa tcaaaagaag
     4621 acgtaaaagt tcttttggaa aacctttatc cgcagtttga aaagctgttt ccagcgatca
     4681 aaggaaaaat tgaagtcggt ttaaaagtaa ttgggaaaaa agaatggctt gagaaaaaag
     4741 taaacgaaaa tcctgaactt gagaaagtat cagcatccgt aaaaggaaaa tcagaagcag
     4801 ccggttatta tgagcgtatt caacttggag gaatggctca aaagatgttt acttccctgc
     4861 aaaaagaagt caagacagat gtgttttctc cgcttgaaga agcagcggaa gcagcaaaag
     4921 caaatgagcc aacgggcgaa acgatgcttt taaacgcgtc tttcttaatt aaccgagaag
     4981 atgaagcgaa gtttgatgaa aaagtaaatg aagcgcatga aaactggaaa gacaaagccg
     5041 attttcatta cagcggtcct tggcctgctt ataattttgt gaacattcgc ctaaaagtag
     5101 aagagaaata acgtgcttca caaattagta accgcaccca ttaaccttgt agtgaaaatc
     5161 ggcgaaaaag tacaggaaga agctgataaa cagctatatg accttccgac gattcagcaa
     5221 aagctcattc agcttcaaat gatgtttgag cttggtgaaa ttccagaaga agcgtttcaa
     5281 gaaaaagaag atgaattgtt aatgaggtac gaaattgcga aacgcagaga aattgaacaa
     5341 tgggaagagc taacacaaaa aagaaatgag gaatcctaga tgggagaatt actgtattta
     5401 tacggtttaa ttccaacaaa agaagcagca gccatagagc cgtttccatc ttataagggg
     5461 tttgacggag aacattcact gtacccaatt gcgtttgatc aggtgacggc tgtagtttct
     5521 aagctggatg ctgacaccta ttcagaaaaa gtgattcaag aaaaaatgga gcaggatatg
     5581 agctggctgc aggaaaaagc atttcatcat cacgaaacgg tagccgcttt gtacgaagaa
     5641 tttacgatca ttccattaaa attttgcacc atttataaag gtgaagaaag tctgcaggca
     5701 gctattgaga ttaacaaaga aaagatagag aattcactga cgctgcttca aggaaatgaa
     5761 gagtggaatg tgaaaattta ctgtgatgat acagagctta aaaaaggaat cagcgaaacg
     5821 aatgaaagcg tgaaagcgaa aaaacaagaa attagtcact tatcaccagg aagacagttt
     5881 tttgaaaaga aaaaaataga tcagctgatt gaaaaagaat tagagcttca caaaaacaaa
     5941 gtgtgtgaag agatacatga caagctaaaa gaattatcgc tttatgactc tgttaaaaag
     6001 aattggagca aagacgtaac tggcgcagct gaacagatgg cgtggaacag cgtgtttctt
     6061 ctcccgtctc tgcagattac taagttcgta aacgaaatag aagagcttca gcaaaggctt
     6121 gaaaataaag gctggaagtt tgaagtgacg ggaccatggc cgccctatca tttctcgagc
     6181 tttgcgtaaa gtgaggaatt aacattatgt ctcttaaaca atccatggag aataaagata
     6241 ttgctcttat tgatatttta gatgtcattt tagataaagg agtcgccatt aaaggagact
     6301 taatcatttc catagctggc gtcgatttag tgtatttgga tttgcgggtg cttatttctt
     6361 cggttgaaac gcttgtgcaa gcaaaagaag gaaatcacaa accaatcact tctgaacaat
     6421 ttgataaaca aaaagaagag ctgatggacg ctacgggcca gcctagtaag tggacgaacc
     6481 ctctaggttc gtgacccttg cgagcagtcg tatgctttct cgaattccga gcggttaagc
     6541 gtgacagtcc aggaggaatt aatagatgca accggtcagc caagcaaatg gacgaatcca
     6601 cttggatcct gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct
     6661 attgaggcaa atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga
     6721 cgaacaaatt gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt
     6781 gaaagaggtg ttcggtctgg atgcagaaga tttaaatatt gatcttggcc cgctaggcag
     6841 cctgctttaa gcggtcagta ggaggaacag tatggcagtc gaacataata tgcagtcaag
     6901 tacgattgta gatgtgctcg aaaagatttt ggataaagga gtcgttatag cgggggacat
     6961 caccgtagga attgcagatg tcgagctatt aacgataaag atccgcttga ttgtggcttc
     7021 ggttgataag gcaaaagaaa tcggcatgga ctggtgggaa aatgatccgt atctcagttc
     7081 aaaaggagcc aataacaaag cgctcgaaga agaaaataaa atgctgcatg agcggttaaa
     7141 aacgcttgaa gaaaaaatag aaacgaaacg ttaaaaactg tacgctactt aaaaaatgga
     7201 gggatttaca atggcaactg aaacaaaatt agataacaca caggcagaaa acaaggaaaa
     7261 taaaaatgcg gaaaacggtt caaaagaaaa gaacggttca aaagcaagca aaacaacaag
     7321 cagcgggcca atcaaacgag cggtagcagg aggcatcatc ggtgcaacga ttggatatgt
     7381 atcgactcct gaaaatcgaa aaagtctcct tgaccgcatt gatacagacg aattaaaaag
     7441 caaagcatct gatttaggaa caaaggtaaa agaaaaatca aaaagcagcg tggccagcct
     7501 gaaaacatct gcgggaagct tgtttaaaaa agataaagat aaatcaaaag atgatgaaga
     7561 aaacgtaaat tcttctagta gcgaaacaga agacgataac gttcaagagt acgacgagtt
     7621 aaaagaagaa aatcaaactc ttcaagatcg cttatcacag cttgaagaaa aaatgaacat
     7681 gcttgttgag cttagcctca ataaaaatca agacgaagaa gcggaagata cagattccga
     7741 cgaagaagag aacgatgaga acgatgaaaa cgatgaaaac gagcaggacg atgaaaacga
     7801 agaagaaaca tctaagccac gtaaaaagga taaaaaagaa gctgaggaag aagaaagtga
     7861 aagtgacgaa gacagcgagg aagaagagga agattctcgc tcaaacaaaa aaaataaaaa
     7921 agtaaaaaca gaagaagaag acgaagatga aagcgaagaa gaaaaaaagg aagcgaaacc
     7981 aaaaaagtca acagctaaaa aatcaaaaaa tacaaaagca aagaaaaaca cggacgaaga
     8041 agatgatgaa gcaacatctc tttctagtga agacgataca acagcctaag acgtaaagga
     8101 ggaaagaaag accataaaaa acggcggggt gattgccccg ccgtttttta gtgatgtgat
     8161 gagatgtgca gcttcttttt tcgatacatg agcggagtca tcttcatttc ttttcgaaac
     8221 tgattaataa aatagcttgt actattaaac cctacttgat aggcgacttc cgtcacattt
     8281 gcttctgctt gctgcagtcc ggattcgcga acgcgtctcg agagatccgg ctgctaacaa
     8341 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     8401 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccgacta
     8461 gtagcccgcc t
//
