LOCUS       Exported                7605 bp ds-DNA     circular SYN 01-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1040)
                     /label=RMpC_RMBB_rev
     primer_bind     complement(1020..1040)
                     /label=RMp2A_RMBB_rev
     primer_bind     1040..1071
                     /label=RMpC_RMBB_fwd
     primer_bind     1041..1071
                     /label=RMp2A_RMBB_fwd
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1101
                     /label=BR
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev (1)
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev (2)
     misc_feature    1407
                     /label=RN
     primer_bind     1408..1427
                     /label=gvpF_fwd
     primer_bind     1408..1427
                     /label=gvpF_fwd (1)
     primer_bind     1408..1427
                     /label=gvpF_fwd (2)
     misc_feature    1408..1416
                     /label=RBS
     misc_feature    1408
                     /label=NF
     CDS             1422..2189
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     1655..1682
                     /label=Bm3seqF
     primer_bind     complement(2154..2174)
                     /label=gvpF_rev
     primer_bind     complement(2154..2174)
                     /label=gvpF_rev (1)
     misc_feature    2174^2175
                     /label=FG
     primer_bind     2175..2197
                     /label=gvpG_fwd
     primer_bind     2175..2197
                     /label=gvpG_fwd (1)
     misc_feature    2179..2187
                     /label=RBS
     CDS             2191..2457
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     2403..2430
                     /label=Bm4seqF
     primer_bind     complement(2421..2442)
                     /label=gvpG_rev
     primer_bind     complement(2421..2442)
                     /label=gvpG_rev (1)
     misc_feature    2442^2443
                     /label=GL
     primer_bind     2443..2462
                     /label=gvpL_fwd
     primer_bind     2443..2462
                     /label=gvpL_fwd (1)
     misc_feature    2444..2452
                     /label=RBS
     CDS             2458..3267
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    2528
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    2597
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3047
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3178..3204
                     /label=Bm5seqF
     primer_bind     complement(3251..3268)
                     /label=gvpL_rev
     primer_bind     complement(3251..3268)
                     /label=gvpL_rev (1)
     misc_feature    3268^3269
                     /label=LS
     primer_bind     3269..3293
                     /label=gvpS_fwd
     primer_bind     3269..3293
                     /label=gvpS_fwd (1)
     misc_feature    3269..3277
                     /label=RBS
     CDS             3285..3572
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(3588..3607)
                     /label=gvpS_rev
     primer_bind     complement(3588..3607)
                     /label=gvpS_rev (1)
     misc_feature    3607^3608
                     /label=SK
     primer_bind     3608..3623
                     /label=gvpK_fwd
     primer_bind     3608..3623
                     /label=gvpK_fwd (1)
     misc_feature    3629..3637
                     /label=RBS
     CDS             3644..3928
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(3919..3934)
                     /label=gvpK_rev
     primer_bind     complement(3919..3934)
                     /label=gvpK_rev (1)
     misc_feature    3934^3935
                     /label=KJ
     primer_bind     3935..3953
                     /label=gvpJ_fwd
     primer_bind     3935..3953
                     /label=gvpJ_fwd (1)
     misc_feature    3938..3946
                     /label=RBS
     CDS             3950..4252
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4075
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4081..4102
                     /label=Bm6seqF
     primer_bind     complement(4247..4268)
                     /label=gvpJ_rev
     primer_bind     complement(4247..4268)
                     /label=gvpJ_rev (1)
     misc_feature    4268^4269
                     /label=JT
     primer_bind     4269..4292
                     /label=gvpT_fwd
     primer_bind     4269..4292
                     /label=gvpT_fwd (1)
     misc_feature    4275..4283
                     /label=RBS
     CDS             4289..5167
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4310..4336
                     /label=Bm6.1seq
     misc_feature    4613
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4676
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4750..4776
                     /label=Bm7seqF
     primer_bind     complement(5155..5172)
                     /label=gvpT_rev
     misc_feature    5172^5173
                     /label=TU
     primer_bind     5173..5193
                     /label=gvpU_fwd
     primer_bind     5173..5193
                     /label=gvpU_fwd (1)
     misc_feature    5175..5183
                     /label=RBS
     CDS             5191..5607
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     5530..5554
                     /label=GvpUseqF
     misc_feature    5760^5761
                     /label=UV
     primer_bind     5761..5778
                     /label=vector_fwd
     primer_bind     5761..5778
                     /label=vector_fwd (1)
     terminator      5877..5924
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5991..6082
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6072..6077
                     /label=Emopec_63.3%
     CDS             6083..6943
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7017..7605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggagg agcttgaaaa catgagtgaa acaaacgaaa
     1441 caggtattta tatttttagc gccattcaaa cggataaaga cgaagaattt ggcgccgtgg
     1501 aagtagaagg aacaaaagct gaaacatttt tgattcgcta caaagacgcg gctatggtag
     1561 cagctgaagt accgatgaaa atttatcatc ctaatcgcca aaatttatta atgcatcaaa
     1621 acgcagtagc agcgattatg gacaagaacg atacggttat tccaatcagc tttgggaatg
     1681 tattcaaatc aaaagaagac gtaaaagttc ttttggaaaa cctttatccg cagtttgaaa
     1741 agctgtttcc agcgatcaaa ggaaaaattg aagtcggttt aaaagtaatt gggaaaaaag
     1801 aatggcttga gaaaaaagta aacgaaaatc ctgaacttga gaaagtatca gcatccgtaa
     1861 aaggaaaatc agaagcagcc ggttattatg agcgtattca acttggagga atggctcaaa
     1921 agatgtttac ttccctgcaa aaagaagtca agacagatgt gttttctccg cttgaagaag
     1981 cagcggaagc agcaaaagca aatgagccaa cgggcgaaac gatgctttta aacgcgtctt
     2041 tcttaattaa ccgagaagat gaagcgaagt ttgatgaaaa agtaaatgaa gcgcatgaaa
     2101 actggaaaga caaagccgat tttcattaca gcggtccttg gcctgcttat aattttgtga
     2161 acattcgcct aaaagtagaa gagaaataac gtgcttcaca aattagtaac cgcacccatt
     2221 aaccttgtag tgaaaatcgg cgaaaaagta caggaagaag ctgataaaca gctatatgac
     2281 cttccgacga ttcagcaaaa gctcattcag cttcaaatga tgtttgagct tggtgaaatt
     2341 ccagaagaag cgtttcaaga aaaagaagat gaattgttaa tgaggtacga aattgcgaaa
     2401 cgcagagaaa ttgaacaatg ggaagagcta acacaaaaaa gaaatgagga atcctagatg
     2461 ggagaattac tgtatttata cggtttaatt ccaacaaaag aagcagcagc catagagccg
     2521 tttccatctt ataaggggtt tgacggagaa cattcactgt acccaattgc gtttgatcag
     2581 gtgacggctg tagtttctaa gctggatgct gacacctatt cagaaaaagt gattcaagaa
     2641 aaaatggagc aggatatgag ctggctgcag gaaaaagcat ttcatcatca cgaaacggta
     2701 gccgctttgt acgaagaatt tacgatcatt ccattaaaat tttgcaccat ttataaaggt
     2761 gaagaaagtc tgcaggcagc tattgagatt aacaaagaaa agatagagaa ttcactgacg
     2821 ctgcttcaag gaaatgaaga gtggaatgtg aaaatttact gtgatgatac agagcttaaa
     2881 aaaggaatca gcgaaacgaa tgaaagcgtg aaagcgaaaa aacaagaaat tagtcactta
     2941 tcaccaggaa gacagttttt tgaaaagaaa aaaatagatc agctgattga aaaagaatta
     3001 gagcttcaca aaaacaaagt gtgtgaagag atacatgaca agctaaaaga attatcgctt
     3061 tatgactctg ttaaaaagaa ttggagcaaa gacgtaactg gcgcagctga acagatggcg
     3121 tggaacagcg tgtttcttct cccgtctctg cagattacta agttcgtaaa cgaaatagaa
     3181 gagcttcagc aaaggcttga aaataaaggc tggaagtttg aagtgacggg accatggccg
     3241 ccctatcatt tctcgagctt tgcgtaaagt gaggaattaa cattatgtct cttaaacaat
     3301 ccatggagaa taaagatatt gctcttattg atattttaga tgtcatttta gataaaggag
     3361 tcgccattaa aggagactta atcatttcca tagctggcgt cgatttagtg tatttggatt
     3421 tgcgggtgct tatttcttcg gttgaaacgc ttgtgcaagc aaaagaagga aatcacaaac
     3481 caatcacttc tgaacaattt gataaacaaa aggaagagtt aatggacgcg accggccagc
     3541 cgagcaaatg gaccaatccg cttggctcct gacccttgcg agcagtcgta tgctttctcg
     3601 aattccgagc ggttaagcgt gacagtccag gaggaattaa tggatgcaac cggtcagcca
     3661 agcaaatgga cgaatccact tggatcctga tcaagctgaa caaggcttag cgcagcttgt
     3721 gatgacagtt attgagctat tgaggcaaat cgttgaacgt catgccatga ggcgggtgga
     3781 gggtggaacg ttgacggacg aacaaattga aaacttagga attgcactaa tgaacttaga
     3841 agaaaaaatg gacgagttga aagaggtgtt cggtctggat gcagaagatt taaatattga
     3901 tcttggaccg ctaggcagcc tgctttaagc ggtcagtagg aggaacagta tggcagtcga
     3961 acataatatg cagtcaagta cgattgtaga tgtgctcgaa aagattttgg ataaaggagt
     4021 cgttatagcg ggggacatca ccgtaggaat tgcagatgtc gagctattaa cgataaagat
     4081 ccgcttgatt gtggcttcgg ttgataaggc aaaagaaatc ggcatggact ggtgggaaaa
     4141 tgatccgtat ctcagttcaa aaggagccaa taacaaagcg ctcgaagaag aaaataaaat
     4201 gctgcatgag cggttaaaaa cgcttgaaga aaaaatagaa acgaaacgtt aaaaactgta
     4261 cgctacttaa aaaatggagg gatttacaat ggcaactgaa acaaaattag ataacacaca
     4321 ggcagaaaac aaggaaaata aaaatgcgga aaacggttca aaagaaaaga acggttcaaa
     4381 agcaagcaaa acaacaagca gcgggccaat caaacgagcg gtagcaggag gcatcatcgg
     4441 tgcaacgatt ggatatgtat cgactcctga aaatcgaaaa agtctccttg accgcattga
     4501 tacagacgaa ttaaaaagca aagcatctga tttaggaaca aaggtaaaag aaaaatcaaa
     4561 aagcagcgtg gccagcctga aaacatctgc gggaagcttg tttaaaaaag ataaagataa
     4621 atcaaaagat gatgaagaaa acgtaaattc ttctagtagc gaaacagaag acgataacgt
     4681 tcaagagtac gacgagttaa aagaagaaaa tcaaactctt caagatcgct tatcacagct
     4741 tgaagaaaaa atgaacatgc ttgttgagct tagcctcaat aaaaatcaag acgaagaagc
     4801 ggaagataca gattccgacg aagaagagaa cgatgagaac gatgaaaacg atgaaaacga
     4861 gcaggacgat gaaaacgaag aagaaacatc taagccacgt aaaaaggata aaaaagaagc
     4921 tgaggaagaa gaaagtgaaa gtgacgaaga cagcgaggaa gaagaggaag attctcgctc
     4981 aaacaaaaaa aataaaaaag taaaaacaga agaagaagac gaagatgaaa gcgaagaaga
     5041 aaaaaaggaa gcgaaaccaa aaaagtcaac agctaaaaaa tcaaaaaata caaaagcaaa
     5101 gaaaaacacg gacgaagaag atgatgaagc aacatctctt tctagtgaag acgatacaac
     5161 agcctaagac gtaaaggagg aaagaaagac atgagtacag gcccttcttt ttcaactaaa
     5221 gacaatacgc ttgaatactt tgtgaaagct tctaataaac acggcttttc acttgatatt
     5281 tcattaaatg taaacggcgc tgtgatttcc ggtaccatga tttcagcaaa agaatacttt
     5341 gattacttaa gcgaaacgtt tgaagaaggc agtgaagtgg ctcaggcgct aagcgaacaa
     5401 ttctctttag caagcgaagc gagcgaatca aacggagaag cagaagccca ttttattcat
     5461 ttgaaaaata caaagattta ctgtggagac agtaaatcta ctccttctaa aggcaaaatc
     5521 ttttggagag ggaaaatagc agaagtagac gggtttttct taggaaagat ttctgatgca
     5581 aaatcaacga gtaaaaagag ttcataaaaa acggcggggt gattgccccg ccgtttttta
     5641 gtgatgtgat gagatgtgca gcttcttttt tcgatacatg agcggagtca tcttcatttc
     5701 ttttcgaaac tgattaataa aatagcttgt actattaaac cctacttgat aggcgacttc
     5761 cgtcacattt gcttctgctt gctgcagtcc ggattcgcga acgcgtctcg agagatccgg
     5821 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     5881 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     5941 tatccgacta gtagcccgcc taatgagcgg gctttttttt aattccccta tttgtttatt
     6001 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6061 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6121 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctcgtga aagtaaaaga
     6181 cgcagaggac caattggggg cacgagtggg atacatagaa ctggacttga atagcggtaa
     6241 aatccttgag agttttcgcc ctgaagagcg ttttccaatg atgagcactt tcaaagttct
     6301 gctatgtgga gcagtattat cccgtgtaga tgcggggcaa gagcaactcg gacgacgaat
     6361 acactattcg cagaatgact tggttgaata ctccccagtg acagaaaagc accttacgga
     6421 cggaatgacg gtaagagaat tatgtagtgc cgccataacg atgagtgata acactgcggc
     6481 gaacttactt ctgacaacca tcggtggacc gaaggaatta accgcttttt tgcacaatat
     6541 gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa ctgaatgaag ccataccaaa
     6601 cgacgagcga gacaccacaa tgcctgcggc aatggcaaca acattacgca aactattaac
     6661 tggcgaacta cttactctgg cttcacggca acaattaata gactggcttg aagcggataa
     6721 agttgcagga ccactactgc gttcggcact tcctgctggc tggtttattg ctgataaatc
     6781 tggggcagga gagcgtggtt cacggggtat cattgccgca cttggaccag atggtaagcc
     6841 ttcccgtatc gtagttatct acacgacggg tagtcaggca actatggacg aacgaaatag
     6901 acagattgct gaaatagggg cttcactgat taagcattgg taaaccgata caattaaagg
     6961 ctccttttgg agcctttttt tttggacgga ccggtagaaa agatcaaagg atcttcttga
     7021 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7081 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7141 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7201 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7261 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7321 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7381 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7441 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7501 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7561 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaa
//
