LOCUS       Exported                7781 bp ds-DNA     circular SYN 10-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1040)
                     /label=RMpC_RMBB_rev
     primer_bind     complement(1020..1040)
                     /label=RMp2A_RMBB_rev
     primer_bind     1040..1071
                     /label=RMpC_RMBB_fwd
     primer_bind     1041..1071
                     /label=RMp2A_RMBB_fwd
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1101
                     /label=BR
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     misc_feature    1407^1408
                     /label=RN
     primer_bind     1408..1428
                     /label=gvpN_fwd
     misc_feature    1409..1417
                     /label=RBS
     CDS             1424..2350
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1454..1479
                     /label=Bm1.1seqF
     misc_feature    1486
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1856..1875
                     /label=Bm2seqF
     primer_bind     complement(2315..2354)
                     /label=G dbfG r
     primer_bind     complement(2321..2350)
                     /label=gvpN_rev
     misc_feature    2350
                     /label=NF
     primer_bind     2351..2373
                     /label=gvpG_fwd
     CDS             2351..2365
                     /codon_start=1
                     /label=GvpF
                     /translation="VEEK"
     misc_feature    2351
                     /label=FG
     misc_feature    2355..2363
                     /label=RBS
     CDS             2367..2633
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     2579..2606
                     /label=Bm4seqF
     primer_bind     2589..2617
                     /label=G dbfG f
     primer_bind     complement(2589..2605)
                     /label=G dbfG r
     primer_bind     complement(2597..2618)
                     /label=gvpG_rev
     misc_feature    2618^2619
                     /label=GL
     primer_bind     2619..2638
                     /label=gvpL_fwd
     misc_feature    2620..2628
                     /label=RBS
     CDS             2634..3443
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    2704
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    2773
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3223
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3354..3380
                     /label=Bm5seqF
     primer_bind     complement(3427..3444)
                     /label=gvpL_rev
     misc_feature    3444^3445
                     /label=LS
     primer_bind     3445..3469
                     /label=gvpS_fwd
     misc_feature    3445..3453
                     /label=RBS
     CDS             3461..3748
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(3764..3783)
                     /label=gvpS_rev
     misc_feature    3783^3784
                     /label=SK
     primer_bind     3784..3799
                     /label=gvpK_fwd
     misc_feature    3805..3813
                     /label=RBS
     CDS             3820..4104
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4095..4110)
                     /label=gvpK_rev
     misc_feature    4110^4111
                     /label=KJ
     primer_bind     4111..4129
                     /label=gvpJ_fwd
     misc_feature    4114..4122
                     /label=RBS
     CDS             4126..4428
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4251
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4257..4278
                     /label=Bm6seqF
     primer_bind     complement(4423..4444)
                     /label=gvpJ_rev
     misc_feature    4444^4445
                     /label=JT
     primer_bind     4445..4468
                     /label=gvpT_fwd
     misc_feature    4451..4459
                     /label=RBS
     CDS             4465..5343
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4486..4512
                     /label=Bm6.1seq
     misc_feature    4789
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4852
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4926..4952
                     /label=Bm7seqF
     primer_bind     complement(5331..5348)
                     /label=gvpT_rev
     misc_feature    5348^5349
                     /label=TU
     primer_bind     5349..5369
                     /label=gvpU_fwd
     misc_feature    5351..5359
                     /label=RBS
     CDS             5367..5783
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     5706..5730
                     /label=GvpUseqF
     misc_feature    5936^5937
                     /label=UV
     primer_bind     5937..5954
                     /label=vector_fwd
     terminator      6053..6100
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6167..6258
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6248..6253
                     /label=Emopec_63.3%
     CDS             6259..7119
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7193..7781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga
     1441 caaaaggaaa aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc
     1501 aagagcgctg agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg
     1561 aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat
     1621 gcacgggaat cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag
     1681 caaaaaagta atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga
     1741 aaactggcag gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga
     1801 cgaatttact cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg
     1861 cgtgctgccg ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt
     1921 ccgcgtcatc ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc
     1981 gcttctcgac aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc
     2041 gattttaacg gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt
     2101 agcaaacgtg cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc
     2161 gcttatgatc gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga
     2221 ttttcaaacg ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga
     2281 aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga
     2341 agaaaagtaa gtagaagaga aataacgtgc ttcacaaatt agtaaccgca cccattaacc
     2401 ttgtagtgaa aatcggcgaa aaagtacagg aagaagctga taaacagcta tatgaccttc
     2461 cgacgattca gcaaaagctc attcagcttc aaatgatgtt tgagcttggt gaaattccag
     2521 aagaagcgtt tcaagaaaaa gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca
     2581 gagaaattga acaatgggaa gagctaacac aaaaaagaaa tgaggaatcc tagatgggag
     2641 aattactgta tttatacggt ttaattccaa caaaagaagc agcagccata gagccgtttc
     2701 catcttataa ggggtttgac ggagaacatt cactgtaccc aattgcgttt gatcaggtga
     2761 cggctgtagt ttctaagctg gatgctgaca cctattcaga aaaagtgatt caagaaaaaa
     2821 tggagcagga tatgagctgg ctgcaggaaa aagcatttca tcatcacgaa acggtagccg
     2881 ctttgtacga agaatttacg atcattccat taaaattttg caccatttat aaaggtgaag
     2941 aaagtctgca ggcagctatt gagattaaca aagaaaagat agagaattca ctgacgctgc
     3001 ttcaaggaaa tgaagagtgg aatgtgaaaa tttactgtga tgatacagag cttaaaaaag
     3061 gaatcagcga aacgaatgaa agcgtgaaag cgaaaaaaca agaaattagt cacttatcac
     3121 caggaagaca gttttttgaa aagaaaaaaa tagatcagct gattgaaaaa gaattagagc
     3181 ttcacaaaaa caaagtgtgt gaagagatac atgacaagct aaaagaatta tcgctttatg
     3241 actctgttaa aaagaattgg agcaaagacg taactggcgc agctgaacag atggcgtgga
     3301 acagcgtgtt tcttctcccg tctctgcaga ttactaagtt cgtaaacgaa atagaagagc
     3361 ttcagcaaag gcttgaaaat aaaggctgga agtttgaagt gacgggacca tggccgccct
     3421 atcatttctc gagctttgcg taaagtgagg aattaacatt atgtctctta aacaatccat
     3481 ggagaataaa gatattgctc ttattgatat tttagatgtc attttagata aaggagtcgc
     3541 cattaaagga gacttaatca tttccatagc tggcgtcgat ttagtgtatt tggatttgcg
     3601 ggtgcttatt tcttcggttg aaacgcttgt gcaagcaaaa gaaggaaatc acaaaccaat
     3661 cacttctgaa caatttgata aacaaaagga agagttaatg gacgcgaccg gccagccgag
     3721 caaatggacc aatccgcttg gctcctgacc cttgcgagca gtcgtatgct ttctcgaatt
     3781 ccgagcggtt aagcgtgaca gtccaggagg aattaatgga tgcaaccggt cagccaagca
     3841 aatggacgaa tccacttgga tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg
     3901 acagttattg agctattgag gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt
     3961 ggaacgttga cggacgaaca aattgaaaac ttaggaattg cactaatgaa cttagaagaa
     4021 aaaatggacg agttgaaaga ggtgttcggt ctggatgcag aagatttaaa tattgatctt
     4081 ggaccgctag gcagcctgct ttaagcggtc agtaggagga acagtatggc agtcgaacat
     4141 aatatgcagt caagtacgat tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt
     4201 atagcggggg acatcaccgt aggaattgca gatgtcgagc tattaacgat aaagatccgc
     4261 ttgattgtgg cttcggttga taaggcaaaa gaaatcggca tggactggtg ggaaaatgat
     4321 ccgtatctca gttcaaaagg agccaataac aaagcgctcg aagaagaaaa taaaatgctg
     4381 catgagcggt taaaaacgct tgaagaaaaa atagaaacga aacgttaaaa actgtacgct
     4441 acttaaaaaa tggagggatt tacaatggca actgaaacaa aattagataa cacacaggca
     4501 gaaaacaagg aaaataaaaa tgcggaaaac ggttcaaaag aaaagaacgg ttcaaaagca
     4561 agcaaaacaa caagcagcgg gccaatcaaa cgagcggtag caggaggcat catcggtgca
     4621 acgattggat atgtatcgac tcctgaaaat cgaaaaagtc tccttgaccg cattgataca
     4681 gacgaattaa aaagcaaagc atctgattta ggaacaaagg taaaagaaaa atcaaaaagc
     4741 agcgtggcca gcctgaaaac atctgcggga agcttgttta aaaaagataa agataaatca
     4801 aaagatgatg aagaaaacgt aaattcttct agtagcgaaa cagaagacga taacgttcaa
     4861 gagtacgacg agttaaaaga agaaaatcaa actcttcaag atcgcttatc acagcttgaa
     4921 gaaaaaatga acatgcttgt tgagcttagc ctcaataaaa atcaagacga agaagcggaa
     4981 gatacagatt ccgacgaaga agagaacgat gagaacgatg aaaacgatga aaacgagcag
     5041 gacgatgaaa acgaagaaga aacatctaag ccacgtaaaa aggataaaaa agaagctgag
     5101 gaagaagaaa gtgaaagtga cgaagacagc gaggaagaag aggaagattc tcgctcaaac
     5161 aaaaaaaata aaaaagtaaa aacagaagaa gaagacgaag atgaaagcga agaagaaaaa
     5221 aaggaagcga aaccaaaaaa gtcaacagct aaaaaatcaa aaaatacaaa agcaaagaaa
     5281 aacacggacg aagaagatga tgaagcaaca tctctttcta gtgaagacga tacaacagcc
     5341 taagacgtaa aggaggaaag aaagacatga gtacaggccc ttctttttca actaaagaca
     5401 atacgcttga atactttgtg aaagcttcta ataaacacgg cttttcactt gatatttcat
     5461 taaatgtaaa cggcgctgtg atttccggta ccatgatttc agcaaaagaa tactttgatt
     5521 acttaagcga aacgtttgaa gaaggcagtg aagtggctca ggcgctaagc gaacaattct
     5581 ctttagcaag cgaagcgagc gaatcaaacg gagaagcaga agcccatttt attcatttga
     5641 aaaatacaaa gatttactgt ggagacagta aatctactcc ttctaaaggc aaaatctttt
     5701 ggagagggaa aatagcagaa gtagacgggt ttttcttagg aaagatttct gatgcaaaat
     5761 caacgagtaa aaagagttca taaaaaacgg cggggtgatt gccccgccgt tttttagtga
     5821 tgtgatgaga tgtgcagctt cttttttcga tacatgagcg gagtcatctt catttctttt
     5881 cgaaactgat taataaaata gcttgtacta ttaaacccta cttgataggc gacttccgtc
     5941 acatttgctt ctgcttgctg cagtccggat tcgcgaacgc gtctcgagag atccggctgc
     6001 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
     6061 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
     6121 cgactagtag cccgcctaat gagcgggctt ttttttaatt cccctatttg tttatttttc
     6181 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6241 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6301 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tcgtgaaagt aaaagacgca
     6361 gaggaccaat tgggggcacg agtgggatac atagaactgg acttgaatag cggtaaaatc
     6421 cttgagagtt ttcgccctga agagcgtttt ccaatgatga gcactttcaa agttctgcta
     6481 tgtggagcag tattatcccg tgtagatgcg gggcaagagc aactcggacg acgaatacac
     6541 tattcgcaga atgacttggt tgaatactcc ccagtgacag aaaagcacct tacggacgga
     6601 atgacggtaa gagaattatg tagtgccgcc ataacgatga gtgataacac tgcggcgaac
     6661 ttacttctga caaccatcgg tggaccgaag gaattaaccg cttttttgca caatatggga
     6721 gaccatgtaa ctcgccttga ccgttgggaa ccagaactga atgaagccat accaaacgac
     6781 gagcgagaca ccacaatgcc tgcggcaatg gcaacaacat tacgcaaact attaactggc
     6841 gaactactta ctctggcttc acggcaacaa ttaatagact ggcttgaagc ggataaagtt
     6901 gcaggaccac tactgcgttc ggcacttcct gctggctggt ttattgctga taaatctggg
     6961 gcaggagagc gtggttcacg gggtatcatt gccgcacttg gaccagatgg taagccttcc
     7021 cgtatcgtag ttatctacac gacgggtagt caggcaacta tggacgaacg aaatagacag
     7081 attgctgaaa taggggcttc actgattaag cattggtaaa ccgatacaat taaaggctcc
     7141 ttttggagcc tttttttttg gacggaccgg tagaaaagat caaaggatct tcttgagatc
     7201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7321 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa a
//
