LOCUS       Exported                8294 bp ds-DNA     circular SYN 26-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1040)
                     /label=RMpC_RMBB_rev
     primer_bind     complement(1020..1040)
                     /label=RMp2A_RMBB_rev
     primer_bind     1040..1071
                     /label=RMpC_RMBB_fwd
     primer_bind     1041..1071
                     /label=RMp2A_RMBB_fwd
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     misc_feature    1101
                     /label=BR
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     misc_feature    1407^1408
                     /label=RN
     primer_bind     1408..1428
                     /label=gvpN_fwd
     misc_feature    1409..1417
                     /label=RBS
     CDS             1424..2350
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1454..1479
                     /label=Bm1.1seqF
     misc_feature    1486
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1856..1875
                     /label=Bm2seqF
     primer_bind     complement(2321..2350)
                     /label=gvpN_rev
     misc_feature    2350^2351
                     /label=NF
     primer_bind     2351..2370
                     /label=gvpF_fwd
     misc_feature    2351..2359
                     /label=RBS
     CDS             2365..3117
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLK"
     primer_bind     2598..2625
                     /label=Bm3seqF
     primer_bind     complement(3097..3117)
                     /label=gvpF_rev
     primer_bind     complement(3100..3130)
                     /label=FL.fix_rev
     misc_feature    3121..3125
                     /label=RBS
     CDS             3121..3125
                     /codon_start=1
                     /label=GvpG
                     /translation="E"
     primer_bind     3131..3160
                     /label=FL.fix_fwd
     CDS             3140..3146
                     /codon_start=1
                     /label=GvpG
                     /translation="S"
     misc_feature    3140..3141
                     /label=RBS
     CDS             3147..3956
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3217
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3286
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3736
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3867..3893
                     /label=Bm5seqF
     primer_bind     complement(3940..3957)
                     /label=gvpL_rev
     misc_feature    3957^3958
                     /label=LS
     primer_bind     3958..3982
                     /label=gvpS_fwd
     misc_feature    3958..3966
                     /label=RBS
     CDS             3974..4261
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(4277..4296)
                     /label=gvpS_rev
     misc_feature    4296^4297
                     /label=SK
     primer_bind     4297..4312
                     /label=gvpK_fwd
     misc_feature    4318..4326
                     /label=RBS
     CDS             4333..4617
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4608..4623)
                     /label=gvpK_rev
     misc_feature    4623^4624
                     /label=KJ
     primer_bind     4624..4642
                     /label=gvpJ_fwd
     misc_feature    4627..4635
                     /label=RBS
     CDS             4639..4941
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4764
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4770..4791
                     /label=Bm6seqF
     primer_bind     complement(4936..4957)
                     /label=gvpJ_rev
     misc_feature    4957^4958
                     /label=JT
     primer_bind     4958..4981
                     /label=gvpT_fwd
     misc_feature    4964..4972
                     /label=RBS
     CDS             4978..5856
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4999..5025
                     /label=Bm6.1seq
     misc_feature    5302
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5365
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5439..5465
                     /label=Bm7seqF
     primer_bind     complement(5844..5861)
                     /label=gvpT_rev
     misc_feature    5861^5862
                     /label=TU
     primer_bind     5862..5882
                     /label=gvpU_fwd
     misc_feature    5864..5872
                     /label=RBS
     CDS             5880..6296
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     6219..6243
                     /label=GvpUseqF
     misc_feature    6449^6450
                     /label=UV
     primer_bind     6450..6467
                     /label=vector_fwd
     terminator      6566..6613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6680..6771
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6761..6766
                     /label=Emopec_63.3%
     CDS             6772..7632
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7706..8294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga
     1441 caaaaggaaa aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc
     1501 aagagcgctg agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg
     1561 aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat
     1621 gcacgggaat cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag
     1681 caaaaaagta atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga
     1741 aaactggcag gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga
     1801 cgaatttact cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg
     1861 cgtgctgccg ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt
     1921 ccgcgtcatc ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc
     1981 gcttctcgac aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc
     2041 gattttaacg gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt
     2101 agcaaacgtg cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc
     2161 gcttatgatc gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga
     2221 ttttcaaacg ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga
     2281 aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga
     2341 agaaaagtaa aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt
     2401 agcgccattc aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa
     2461 gctgaaacat ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg
     2521 aaaatttatc atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt
     2581 atggacaaga acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa
     2641 gacgtaaaag ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc
     2701 aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa
     2761 gtaaacgaaa atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca
     2821 gccggttatt atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg
     2881 caaaaagaag tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa
     2941 gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa
     3001 gatgaagcga agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc
     3061 gattttcatt acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta
     3121 gaggaaaaat aatcaggaga tcctagatgg gagaattact gtatttatac ggtttaattc
     3181 caacaaaaga agcagcagcc atagagccgt ttccatctta taaggggttt gacggagaac
     3241 attcactgta cccaattgcg tttgatcagg tgacggctgt agtttctaag ctggatgctg
     3301 acacctattc agaaaaagtg attcaagaaa aaatggagca ggatatgagc tggctgcagg
     3361 aaaaagcatt tcatcatcac gaaacggtag ccgctttgta cgaagaattt acgatcattc
     3421 cattaaaatt ttgcaccatt tataaaggtg aagaaagtct gcaggcagct attgagatta
     3481 acaaagaaaa gatagagaat tcactgacgc tgcttcaagg aaatgaagag tggaatgtga
     3541 aaatttactg tgatgataca gagcttaaaa aaggaatcag cgaaacgaat gaaagcgtga
     3601 aagcgaaaaa acaagaaatt agtcacttat caccaggaag acagtttttt gaaaagaaaa
     3661 aaatagatca gctgattgaa aaagaattag agcttcacaa aaacaaagtg tgtgaagaga
     3721 tacatgacaa gctaaaagaa ttatcgcttt atgactctgt taaaaagaat tggagcaaag
     3781 acgtaactgg cgcagctgaa cagatggcgt ggaacagcgt gtttcttctc ccgtctctgc
     3841 agattactaa gttcgtaaac gaaatagaag agcttcagca aaggcttgaa aataaaggct
     3901 ggaagtttga agtgacggga ccatggccgc cctatcattt ctcgagcttt gcgtaaagtg
     3961 aggaattaac attatgtctc ttaaacaatc catggagaat aaagatattg ctcttattga
     4021 tattttagat gtcattttag ataaaggagt cgccattaaa ggagacttaa tcatttccat
     4081 agctggcgtc gatttagtgt atttggattt gcgggtgctt atttcttcgg ttgaaacgct
     4141 tgtgcaagca aaagaaggaa atcacaaacc aatcacttct gaacaatttg ataaacaaaa
     4201 ggaagagtta atggacgcga ccggccagcc gagcaaatgg accaatccgc ttggctcctg
     4261 acccttgcga gcagtcgtat gctttctcga attccgagcg gttaagcgtg acagtccagg
     4321 aggaattaat ggatgcaacc ggtcagccaa gcaaatggac gaatccactt ggatcctgat
     4381 caagctgaac aaggcttagc gcagcttgtg atgacagtta ttgagctatt gaggcaaatc
     4441 gttgaacgtc atgccatgag gcgggtggag ggtggaacgt tgacggacga acaaattgaa
     4501 aacttaggaa ttgcactaat gaacttagaa gaaaaaatgg acgagttgaa agaggtgttc
     4561 ggtctggatg cagaagattt aaatattgat cttggaccgc taggcagcct gctttaagcg
     4621 gtcagtagga ggaacagtat ggcagtcgaa cataatatgc agtcaagtac gattgtagat
     4681 gtgctcgaaa agattttgga taaaggagtc gttatagcgg gggacatcac cgtaggaatt
     4741 gcagatgtcg agctattaac gataaagatc cgcttgattg tggcttcggt tgataaggca
     4801 aaagaaatcg gcatggactg gtgggaaaat gatccgtatc tcagttcaaa aggagccaat
     4861 aacaaagcgc tcgaagaaga aaataaaatg ctgcatgagc ggttaaaaac gcttgaagaa
     4921 aaaatagaaa cgaaacgtta aaaactgtac gctacttaaa aaatggaggg atttacaatg
     4981 gcaactgaaa caaaattaga taacacacag gcagaaaaca aggaaaataa aaatgcggaa
     5041 aacggttcaa aagaaaagaa cggttcaaaa gcaagcaaaa caacaagcag cgggccaatc
     5101 aaacgagcgg tagcaggagg catcatcggt gcaacgattg gatatgtatc gactcctgaa
     5161 aatcgaaaaa gtctccttga ccgcattgat acagacgaat taaaaagcaa agcatctgat
     5221 ttaggaacaa aggtaaaaga aaaatcaaaa agcagcgtgg ccagcctgaa aacatctgcg
     5281 ggaagcttgt ttaaaaaaga taaagataaa tcaaaagatg atgaagaaaa cgtaaattct
     5341 tctagtagcg aaacagaaga cgataacgtt caagagtacg acgagttaaa agaagaaaat
     5401 caaactcttc aagatcgctt atcacagctt gaagaaaaaa tgaacatgct tgttgagctt
     5461 agcctcaata aaaatcaaga cgaagaagcg gaagatacag attccgacga agaagagaac
     5521 gatgagaacg atgaaaacga tgaaaacgag caggacgatg aaaacgaaga agaaacatct
     5581 aagccacgta aaaaggataa aaaagaagct gaggaagaag aaagtgaaag tgacgaagac
     5641 agcgaggaag aagaggaaga ttctcgctca aacaaaaaaa ataaaaaagt aaaaacagaa
     5701 gaagaagacg aagatgaaag cgaagaagaa aaaaaggaag cgaaaccaaa aaagtcaaca
     5761 gctaaaaaat caaaaaatac aaaagcaaag aaaaacacgg acgaagaaga tgatgaagca
     5821 acatctcttt ctagtgaaga cgatacaaca gcctaagacg taaaggagga aagaaagaca
     5881 tgagtacagg cccttctttt tcaactaaag acaatacgct tgaatacttt gtgaaagctt
     5941 ctaataaaca cggcttttca cttgatattt cattaaatgt aaacggcgct gtgatttccg
     6001 gtaccatgat ttcagcaaaa gaatactttg attacttaag cgaaacgttt gaagaaggca
     6061 gtgaagtggc tcaggcgcta agcgaacaat tctctttagc aagcgaagcg agcgaatcaa
     6121 acggagaagc agaagcccat tttattcatt tgaaaaatac aaagatttac tgtggagaca
     6181 gtaaatctac tccttctaaa ggcaaaatct tttggagagg gaaaatagca gaagtagacg
     6241 ggtttttctt aggaaagatt tctgatgcaa aatcaacgag taaaaagagt tcataaaaaa
     6301 cggcggggtg attgccccgc cgttttttag tgatgtgatg agatgtgcag cttctttttt
     6361 cgatacatga gcggagtcat cttcatttct tttcgaaact gattaataaa atagcttgta
     6421 ctattaaacc ctacttgata ggcgacttcc gtcacatttg cttctgcttg ctgcagtccg
     6481 gattcgcgaa cgcgtctcga gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     6541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     6601 gaggggtttt ttgctgaaag gaggaactat atccgactag tagcccgcct aatgagcggg
     6661 ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6841 cacccagaaa cgctcgtgaa agtaaaagac gcagaggacc aattgggggc acgagtggga
     6901 tacatagaac tggacttgaa tagcggtaaa atccttgaga gttttcgccc tgaagagcgt
     6961 tttccaatga tgagcacttt caaagttctg ctatgtggag cagtattatc ccgtgtagat
     7021 gcggggcaag agcaactcgg acgacgaata cactattcgc agaatgactt ggttgaatac
     7081 tccccagtga cagaaaagca ccttacggac ggaatgacgg taagagaatt atgtagtgcc
     7141 gccataacga tgagtgataa cactgcggcg aacttacttc tgacaaccat cggtggaccg
     7201 aaggaattaa ccgctttttt gcacaatatg ggagaccatg taactcgcct tgaccgttgg
     7261 gaaccagaac tgaatgaagc cataccaaac gacgagcgag acaccacaat gcctgcggca
     7321 atggcaacaa cattacgcaa actattaact ggcgaactac ttactctggc ttcacggcaa
     7381 caattaatag actggcttga agcggataaa gttgcaggac cactactgcg ttcggcactt
     7441 cctgctggct ggtttattgc tgataaatct ggggcaggag agcgtggttc acggggtatc
     7501 attgccgcac ttggaccaga tggtaagcct tcccgtatcg tagttatcta cacgacgggt
     7561 agtcaggcaa ctatggacga acgaaataga cagattgctg aaataggggc ttcactgatt
     7621 aagcattggt aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacggac
     7681 cggtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     8041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     8101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     8161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     8221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8281 ggagcctatg gaaa
//
