LOCUS       Exported                7738 bp ds-DNA     circular SYN 06-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1101
                     /label=BR
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     misc_feature    1407^1408
                     /label=RN
     primer_bind     1408..1428
                     /label=gvpN_fwd
     misc_feature    1409..1417
                     /label=RBS
     CDS             1424..2350
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1454..1479
                     /label=Bm1.1seqF
     misc_feature    1486
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1856..1875
                     /label=Bm2seqF
     primer_bind     complement(2321..2350)
                     /label=gvpN_rev
     misc_feature    2350^2351
                     /label=NF
     primer_bind     2351..2370
                     /label=gvpF_fwd
     misc_feature    2351..2359
                     /label=RBS
     CDS             2365..3132
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2598..2625
                     /label=Bm3seqF
     primer_bind     complement(3097..3117)
                     /label=gvpF_rev
     misc_feature    3117^3118
                     /label=FG
     primer_bind     3118..3140
                     /label=gvpG_fwd
     misc_feature    3122..3130
                     /label=RBS
     CDS             3134..3400
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3346..3373
                     /label=Bm4seqF
     primer_bind     complement(3364..3385)
                     /label=gvpG_rev
     primer_bind     complement(3377..3400)
                     /label=M dL r
     misc_feature    3385^3386
                     /label=GL
     primer_bind     3386..3401
                     /label=gvpL_fwd
     misc_feature    3387..3395
                     /label=RBS
     primer_bind     3401..3422
                     /label=M dL f
     misc_feature    3401^3402
                     /label=LS
     primer_bind     3402..3426
                     /label=gvpS_fwd
     misc_feature    3402..3410
                     /label=RBS
     CDS             3418..3705
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(3721..3740)
                     /label=gvpS_rev
     misc_feature    3740^3741
                     /label=SK
     primer_bind     3741..3756
                     /label=gvpK_fwd
     misc_feature    3762..3770
                     /label=RBS
     CDS             3777..4061
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4052..4067)
                     /label=gvpK_rev
     misc_feature    4067^4068
                     /label=KJ
     primer_bind     4068..4086
                     /label=gvpJ_fwd
     misc_feature    4071..4079
                     /label=RBS
     CDS             4083..4385
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4208
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4214..4235
                     /label=Bm6seqF
     primer_bind     complement(4380..4401)
                     /label=gvpJ_rev
     misc_feature    4401^4402
                     /label=JT
     primer_bind     4402..4425
                     /label=gvpT_fwd
     misc_feature    4408..4416
                     /label=RBS
     CDS             4422..5300
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4443..4469
                     /label=Bm6.1seq
     misc_feature    4746
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4809
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4883..4909
                     /label=Bm7seqF
     primer_bind     complement(5288..5305)
                     /label=gvpT_rev
     misc_feature    5305^5306
                     /label=TU
     primer_bind     5306..5326
                     /label=gvpU_fwd
     misc_feature    5308..5316
                     /label=RBS
     CDS             5324..5740
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     5663..5687
                     /label=GvpUseqF
     misc_feature    5893^5894
                     /label=UV
     primer_bind     5894..5911
                     /label=vector_fwd
     terminator      6010..6057
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6124..6215
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6205..6210
                     /label=Emopec_63.3%
     CDS             6216..7076
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7150..7738
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga
     1441 caaaaggaaa aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc
     1501 aagagcgctg agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg
     1561 aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat
     1621 gcacgggaat cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag
     1681 caaaaaagta atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga
     1741 aaactggcag gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga
     1801 cgaatttact cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg
     1861 cgtgctgccg ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt
     1921 ccgcgtcatc ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc
     1981 gcttctcgac aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc
     2041 gattttaacg gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt
     2101 agcaaacgtg cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc
     2161 gcttatgatc gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga
     2221 ttttcaaacg ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga
     2281 aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga
     2341 agaaaagtaa aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt
     2401 agcgccattc aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa
     2461 gctgaaacat ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg
     2521 aaaatttatc atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt
     2581 atggacaaga acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa
     2641 gacgtaaaag ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc
     2701 aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa
     2761 gtaaacgaaa atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca
     2821 gccggttatt atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg
     2881 caaaaagaag tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa
     2941 gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa
     3001 gatgaagcga agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc
     3061 gattttcatt acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta
     3121 gaagagaaat aacgtgcttc acaaattagt aaccgcaccc attaaccttg tagtgaaaat
     3181 cggcgaaaaa gtacaggaag aagctgataa acagctatat gaccttccga cgattcagca
     3241 aaagctcatt cagcttcaaa tgatgtttga gcttggtgaa attccagaag aagcgtttca
     3301 agaaaaagaa gatgaattgt taatgaggta cgaaattgcg aaacgcagag aaattgaaca
     3361 atgggaagag ctaacacaaa aaagaaatga ggaatcctag agtgaggaat taacattatg
     3421 tctcttaaac aatccatgga gaataaagat attgctctta ttgatatttt agatgtcatt
     3481 ttagataaag gagtcgccat taaaggagac ttaatcattt ccatagctgg cgtcgattta
     3541 gtgtatttgg atttgcgggt gcttatttct tcggttgaaa cgcttgtgca agcaaaagaa
     3601 ggaaatcaca aaccaatcac ttctgaacaa tttgataaac aaaaggaaga gttaatggac
     3661 gcgaccggcc agccgagcaa atggaccaat ccgcttggct cctgaccctt gcgagcagtc
     3721 gtatgctttc tcgaattccg agcggttaag cgtgacagtc caggaggaat taatggatgc
     3781 aaccggtcag ccaagcaaat ggacgaatcc acttggatcc tgatcaagct gaacaaggct
     3841 tagcgcagct tgtgatgaca gttattgagc tattgaggca aatcgttgaa cgtcatgcca
     3901 tgaggcgggt ggagggtgga acgttgacgg acgaacaaat tgaaaactta ggaattgcac
     3961 taatgaactt agaagaaaaa atggacgagt tgaaagaggt gttcggtctg gatgcagaag
     4021 atttaaatat tgatcttgga ccgctaggca gcctgcttta agcggtcagt aggaggaaca
     4081 gtatggcagt cgaacataat atgcagtcaa gtacgattgt agatgtgctc gaaaagattt
     4141 tggataaagg agtcgttata gcgggggaca tcaccgtagg aattgcagat gtcgagctat
     4201 taacgataaa gatccgcttg attgtggctt cggttgataa ggcaaaagaa atcggcatgg
     4261 actggtggga aaatgatccg tatctcagtt caaaaggagc caataacaaa gcgctcgaag
     4321 aagaaaataa aatgctgcat gagcggttaa aaacgcttga agaaaaaata gaaacgaaac
     4381 gttaaaaact gtacgctact taaaaaatgg agggatttac aatggcaact gaaacaaaat
     4441 tagataacac acaggcagaa aacaaggaaa ataaaaatgc ggaaaacggt tcaaaagaaa
     4501 agaacggttc aaaagcaagc aaaacaacaa gcagcgggcc aatcaaacga gcggtagcag
     4561 gaggcatcat cggtgcaacg attggatatg tatcgactcc tgaaaatcga aaaagtctcc
     4621 ttgaccgcat tgatacagac gaattaaaaa gcaaagcatc tgatttagga acaaaggtaa
     4681 aagaaaaatc aaaaagcagc gtggccagcc tgaaaacatc tgcgggaagc ttgtttaaaa
     4741 aagataaaga taaatcaaaa gatgatgaag aaaacgtaaa ttcttctagt agcgaaacag
     4801 aagacgataa cgttcaagag tacgacgagt taaaagaaga aaatcaaact cttcaagatc
     4861 gcttatcaca gcttgaagaa aaaatgaaca tgcttgttga gcttagcctc aataaaaatc
     4921 aagacgaaga agcggaagat acagattccg acgaagaaga gaacgatgag aacgatgaaa
     4981 acgatgaaaa cgagcaggac gatgaaaacg aagaagaaac atctaagcca cgtaaaaagg
     5041 ataaaaaaga agctgaggaa gaagaaagtg aaagtgacga agacagcgag gaagaagagg
     5101 aagattctcg ctcaaacaaa aaaaataaaa aagtaaaaac agaagaagaa gacgaagatg
     5161 aaagcgaaga agaaaaaaag gaagcgaaac caaaaaagtc aacagctaaa aaatcaaaaa
     5221 atacaaaagc aaagaaaaac acggacgaag aagatgatga agcaacatct ctttctagtg
     5281 aagacgatac aacagcctaa gacgtaaagg aggaaagaaa gacatgagta caggcccttc
     5341 tttttcaact aaagacaata cgcttgaata ctttgtgaaa gcttctaata aacacggctt
     5401 ttcacttgat atttcattaa atgtaaacgg cgctgtgatt tccggtacca tgatttcagc
     5461 aaaagaatac tttgattact taagcgaaac gtttgaagaa ggcagtgaag tggctcaggc
     5521 gctaagcgaa caattctctt tagcaagcga agcgagcgaa tcaaacggag aagcagaagc
     5581 ccattttatt catttgaaaa atacaaagat ttactgtgga gacagtaaat ctactccttc
     5641 taaaggcaaa atcttttgga gagggaaaat agcagaagta gacgggtttt tcttaggaaa
     5701 gatttctgat gcaaaatcaa cgagtaaaaa gagttcataa aaaacggcgg ggtgattgcc
     5761 ccgccgtttt ttagtgatgt gatgagatgt gcagcttctt ttttcgatac atgagcggag
     5821 tcatcttcat ttcttttcga aactgattaa taaaatagct tgtactatta aaccctactt
     5881 gataggcgac ttccgtcaca tttgcttctg cttgctgcag tccggattcg cgaacgcgtc
     5941 tcgagagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     6001 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     6061 aaaggaggaa ctatatccga ctagtagccc gcctaatgag cgggcttttt tttaattccc
     6121 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6181 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6241 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctcg
     6301 tgaaagtaaa agacgcagag gaccaattgg gggcacgagt gggatacata gaactggact
     6361 tgaatagcgg taaaatcctt gagagttttc gccctgaaga gcgttttcca atgatgagca
     6421 ctttcaaagt tctgctatgt ggagcagtat tatcccgtgt agatgcgggg caagagcaac
     6481 tcggacgacg aatacactat tcgcagaatg acttggttga atactcccca gtgacagaaa
     6541 agcaccttac ggacggaatg acggtaagag aattatgtag tgccgccata acgatgagtg
     6601 ataacactgc ggcgaactta cttctgacaa ccatcggtgg accgaaggaa ttaaccgctt
     6661 ttttgcacaa tatgggagac catgtaactc gccttgaccg ttgggaacca gaactgaatg
     6721 aagccatacc aaacgacgag cgagacacca caatgcctgc ggcaatggca acaacattac
     6781 gcaaactatt aactggcgaa ctacttactc tggcttcacg gcaacaatta atagactggc
     6841 ttgaagcgga taaagttgca ggaccactac tgcgttcggc acttcctgct ggctggttta
     6901 ttgctgataa atctggggca ggagagcgtg gttcacgggg tatcattgcc gcacttggac
     6961 cagatggtaa gccttcccgt atcgtagtta tctacacgac gggtagtcag gcaactatgg
     7021 acgaacgaaa tagacagatt gctgaaatag gggcttcact gattaagcat tggtaaaccg
     7081 atacaattaa aggctccttt tggagccttt ttttttggac ggaccggtag aaaagatcaa
     7141 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7201 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7261 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7321 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7381 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7441 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7501 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7561 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7621 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7681 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaa
//
