LOCUS       Exported                8209 bp ds-DNA     circular SYN 26-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1040)
                     /label=RMpC_RMBB_rev
     primer_bind     complement(1020..1040)
                     /label=RMp2A_RMBB_rev
     primer_bind     1040..1071
                     /label=RMpC_RMBB_fwd
     primer_bind     1041..1071
                     /label=RMp2A_RMBB_fwd
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1101
                     /label=BR
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     misc_feature    1407^1408
                     /label=RN
     primer_bind     1408..1428
                     /label=gvpN_fwd
     misc_feature    1409..1417
                     /label=RBS
     CDS             1424..2350
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1454..1479
                     /label=Bm1.1seqF
     misc_feature    1486
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1856..1875
                     /label=Bm2seqF
     primer_bind     complement(2321..2350)
                     /label=gvpN_rev
     misc_feature    2350^2351
                     /label=NF
     primer_bind     2351..2370
                     /label=gvpF_fwd
     misc_feature    2351..2359
                     /label=RBS
     CDS             2365..3132
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2598..2625
                     /label=Bm3seqF
     primer_bind     complement(3097..3117)
                     /label=gvpF_rev
     misc_feature    3117^3118
                     /label=FG
     primer_bind     3118..3140
                     /label=gvpG_fwd
     misc_feature    3122..3130
                     /label=RBS
     CDS             3134..3400
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3346..3373
                     /label=Bm4seqF
     primer_bind     complement(3364..3385)
                     /label=gvpG_rev
     misc_feature    3385^3386
                     /label=GL
     primer_bind     3386..3405
                     /label=gvpL_fwd
     misc_feature    3387..3395
                     /label=RBS
     CDS             3401..4210
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3471
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3540
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3990
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4121..4147
                     /label=Bm5seqF
     primer_bind     complement(4194..4211)
                     /label=gvpL_rev
     misc_feature    4211
                     /label=LS
     primer_bind     4212..4227
                     /label=gvpK_fwd
     misc_feature    4212
                     /label=SK
     misc_feature    4233..4241
                     /label=RBS
     CDS             4248..4532
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4523..4538)
                     /label=gvpK_rev
     misc_feature    4538^4539
                     /label=KJ
     primer_bind     4539..4557
                     /label=gvpJ_fwd
     misc_feature    4542..4550
                     /label=RBS
     CDS             4554..4856
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4679
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4685..4706
                     /label=Bm6seqF
     primer_bind     complement(4851..4872)
                     /label=gvpJ_rev
     misc_feature    4872^4873
                     /label=JT
     primer_bind     4873..4896
                     /label=gvpT_fwd
     misc_feature    4879..4887
                     /label=RBS
     CDS             4893..5771
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4914..4940
                     /label=Bm6.1seq
     misc_feature    5217
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5280
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5354..5380
                     /label=Bm7seqF
     primer_bind     complement(5759..5776)
                     /label=gvpT_rev
     misc_feature    5776^5777
                     /label=TU
     primer_bind     5777..5797
                     /label=gvpU_fwd
     misc_feature    5779..5787
                     /label=RBS
     CDS             5795..6211
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     6134..6158
                     /label=GvpUseqF
     misc_feature    6364^6365
                     /label=UV
     primer_bind     6365..6382
                     /label=vector_fwd
     terminator      6481..6528
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6595..6686
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6676..6681
                     /label=Emopec_63.3%
     CDS             6687..7547
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7621..8209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga
     1441 caaaaggaaa aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc
     1501 aagagcgctg agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg
     1561 aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat
     1621 gcacgggaat cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag
     1681 caaaaaagta atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga
     1741 aaactggcag gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga
     1801 cgaatttact cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg
     1861 cgtgctgccg ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt
     1921 ccgcgtcatc ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc
     1981 gcttctcgac aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc
     2041 gattttaacg gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt
     2101 agcaaacgtg cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc
     2161 gcttatgatc gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga
     2221 ttttcaaacg ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga
     2281 aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga
     2341 agaaaagtaa aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt
     2401 agcgccattc aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa
     2461 gctgaaacat ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg
     2521 aaaatttatc atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt
     2581 atggacaaga acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa
     2641 gacgtaaaag ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc
     2701 aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa
     2761 gtaaacgaaa atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca
     2821 gccggttatt atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg
     2881 caaaaagaag tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa
     2941 gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa
     3001 gatgaagcga agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc
     3061 gattttcatt acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta
     3121 gaagagaaat aacgtgcttc acaaattagt aaccgcaccc attaaccttg tagtgaaaat
     3181 cggcgaaaaa gtacaggaag aagctgataa acagctatat gaccttccga cgattcagca
     3241 aaagctcatt cagcttcaaa tgatgtttga gcttggtgaa attccagaag aagcgtttca
     3301 agaaaaagaa gatgaattgt taatgaggta cgaaattgcg aaacgcagag aaattgaaca
     3361 atgggaagag ctaacacaaa aaagaaatga ggaatcctag atgggagaat tactgtattt
     3421 atacggttta attccaacaa aagaagcagc agccatagag ccgtttccat cttataaggg
     3481 gtttgacgga gaacattcac tgtacccaat tgcgtttgat caggtgacgg ctgtagtttc
     3541 taagctggat gctgacacct attcagaaaa agtgattcaa gaaaaaatgg agcaggatat
     3601 gagctggctg caggaaaaag catttcatca tcacgaaacg gtagccgctt tgtacgaaga
     3661 atttacgatc attccattaa aattttgcac catttataaa ggtgaagaaa gtctgcaggc
     3721 agctattgag attaacaaag aaaagataga gaattcactg acgctgcttc aaggaaatga
     3781 agagtggaat gtgaaaattt actgtgatga tacagagctt aaaaaaggaa tcagcgaaac
     3841 gaatgaaagc gtgaaagcga aaaaacaaga aattagtcac ttatcaccag gaagacagtt
     3901 ttttgaaaag aaaaaaatag atcagctgat tgaaaaagaa ttagagcttc acaaaaacaa
     3961 agtgtgtgaa gagatacatg acaagctaaa agaattatcg ctttatgact ctgttaaaaa
     4021 gaattggagc aaagacgtaa ctggcgcagc tgaacagatg gcgtggaaca gcgtgtttct
     4081 tctcccgtct ctgcagatta ctaagttcgt aaacgaaata gaagagcttc agcaaaggct
     4141 tgaaaataaa ggctggaagt ttgaagtgac gggaccatgg ccgccctatc atttctcgag
     4201 ctttgcgtaa aagcggttaa gcgtgacagt ccaggaggaa ttaatggatg caaccggtca
     4261 gccaagcaaa tggacgaatc cacttggatc ctgatcaagc tgaacaaggc ttagcgcagc
     4321 ttgtgatgac agttattgag ctattgaggc aaatcgttga acgtcatgcc atgaggcggg
     4381 tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt aggaattgca ctaatgaact
     4441 tagaagaaaa aatggacgag ttgaaagagg tgttcggtct ggatgcagaa gatttaaata
     4501 ttgatcttgg accgctaggc agcctgcttt aagcggtcag taggaggaac agtatggcag
     4561 tcgaacataa tatgcagtca agtacgattg tagatgtgct cgaaaagatt ttggataaag
     4621 gagtcgttat agcgggggac atcaccgtag gaattgcaga tgtcgagcta ttaacgataa
     4681 agatccgctt gattgtggct tcggttgata aggcaaaaga aatcggcatg gactggtggg
     4741 aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa agcgctcgaa gaagaaaata
     4801 aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat agaaacgaaa cgttaaaaac
     4861 tgtacgctac ttaaaaaatg gagggattta caatggcaac tgaaacaaaa ttagataaca
     4921 cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg ttcaaaagaa aagaacggtt
     4981 caaaagcaag caaaacaaca agcagcgggc caatcaaacg agcggtagca ggaggcatca
     5041 tcggtgcaac gattggatat gtatcgactc ctgaaaatcg aaaaagtctc cttgaccgca
     5101 ttgatacaga cgaattaaaa agcaaagcat ctgatttagg aacaaaggta aaagaaaaat
     5161 caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag cttgtttaaa aaagataaag
     5221 ataaatcaaa agatgatgaa gaaaacgtaa attcttctag tagcgaaaca gaagacgata
     5281 acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac tcttcaagat cgcttatcac
     5341 agcttgaaga aaaaatgaac atgcttgttg agcttagcct caataaaaat caagacgaag
     5401 aagcggaaga tacagattcc gacgaagaag agaacgatga gaacgatgaa aacgatgaaa
     5461 acgagcagga cgatgaaaac gaagaagaaa catctaagcc acgtaaaaag gataaaaaag
     5521 aagctgagga agaagaaagt gaaagtgacg aagacagcga ggaagaagag gaagattctc
     5581 gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga agacgaagat gaaagcgaag
     5641 aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa aaaatcaaaa aatacaaaag
     5701 caaagaaaaa cacggacgaa gaagatgatg aagcaacatc tctttctagt gaagacgata
     5761 caacagccta agacgtaaag gaggaaagaa agacatgagt acaggccctt ctttttcaac
     5821 taaagacaat acgcttgaat actttgtgaa agcttctaat aaacacggct tttcacttga
     5881 tatttcatta aatgtaaacg gcgctgtgat ttccggtacc atgatttcag caaaagaata
     5941 ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa gtggctcagg cgctaagcga
     6001 acaattctct ttagcaagcg aagcgagcga atcaaacgga gaagcagaag cccattttat
     6061 tcatttgaaa aatacaaaga tttactgtgg agacagtaaa tctactcctt ctaaaggcaa
     6121 aatcttttgg agagggaaaa tagcagaagt agacgggttt ttcttaggaa agatttctga
     6181 tgcaaaatca acgagtaaaa agagttcata aaaaacggcg gggtgattgc cccgccgttt
     6241 tttagtgatg tgatgagatg tgcagcttct tttttcgata catgagcgga gtcatcttca
     6301 tttcttttcg aaactgatta ataaaatagc ttgtactatt aaaccctact tgataggcga
     6361 cttccgtcac atttgcttct gcttgctgca gtccggattc gcgaacgcgt ctcgagagat
     6421 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     6481 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     6541 actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt
     6601 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6661 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6721 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctc gtgaaagtaa
     6781 aagacgcaga ggaccaattg ggggcacgag tgggatacat agaactggac ttgaatagcg
     6841 gtaaaatcct tgagagtttt cgccctgaag agcgttttcc aatgatgagc actttcaaag
     6901 ttctgctatg tggagcagta ttatcccgtg tagatgcggg gcaagagcaa ctcggacgac
     6961 gaatacacta ttcgcagaat gacttggttg aatactcccc agtgacagaa aagcacctta
     7021 cggacggaat gacggtaaga gaattatgta gtgccgccat aacgatgagt gataacactg
     7081 cggcgaactt acttctgaca accatcggtg gaccgaagga attaaccgct tttttgcaca
     7141 atatgggaga ccatgtaact cgccttgacc gttgggaacc agaactgaat gaagccatac
     7201 caaacgacga gcgagacacc acaatgcctg cggcaatggc aacaacatta cgcaaactat
     7261 taactggcga actacttact ctggcttcac ggcaacaatt aatagactgg cttgaagcgg
     7321 ataaagttgc aggaccacta ctgcgttcgg cacttcctgc tggctggttt attgctgata
     7381 aatctggggc aggagagcgt ggttcacggg gtatcattgc cgcacttgga ccagatggta
     7441 agccttcccg tatcgtagtt atctacacga cgggtagtca ggcaactatg gacgaacgaa
     7501 atagacagat tgctgaaata ggggcttcac tgattaagca ttggtaaacc gatacaatta
     7561 aaggctcctt ttggagcctt tttttttgga cggaccggta gaaaagatca aaggatcttc
     7621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7741 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     7801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
