LOCUS       Exported                8221 bp ds-DNA     circular SYN 01-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8221)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8221
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1020..1040)
                     /label=RMpC_RMBB_rev
     primer_bind     complement(1020..1040)
                     /label=RMp2A_RMBB_rev
     primer_bind     1040..1071
                     /label=RMpC_RMBB_fwd
     primer_bind     1041..1071
                     /label=RMp2A_RMBB_fwd
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1123
                     /label=gvpR_fwd
     primer_bind     1101
                     /label=Bm1seq
     misc_feature    1101
                     /label=BR
     misc_feature    1114..1122
                     /label=RBS
     CDS             1129..1395
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1386..1407)
                     /label=gvpR_rev
     misc_feature    1407^1408
                     /label=RN
     primer_bind     1408..1428
                     /label=gvpN_fwd
     misc_feature    1409..1417
                     /label=RBS
     CDS             1424..2350
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1454..1479
                     /label=Bm1.1seqF
     misc_feature    1486
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1856..1875
                     /label=Bm2seqF
     primer_bind     complement(2321..2350)
                     /label=gvpN_rev
     misc_feature    2350^2351
                     /label=NF
     primer_bind     2351..2370
                     /label=gvpF_fwd
     misc_feature    2351..2359
                     /label=RBS
     CDS             2365..3132
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2598..2625
                     /label=Bm3seqF
     primer_bind     complement(3097..3117)
                     /label=gvpF_rev
     misc_feature    3117^3118
                     /label=FG
     primer_bind     3118..3140
                     /label=gvpG_fwd
     misc_feature    3122..3130
                     /label=RBS
     CDS             3134..3400
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3346..3373
                     /label=Bm4seqF
     primer_bind     complement(3364..3385)
                     /label=gvpG_rev
     misc_feature    3385^3386
                     /label=GL
     primer_bind     3386..3405
                     /label=gvpL_fwd
     misc_feature    3387..3395
                     /label=RBS
     CDS             3401..4210
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3471
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3540
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3990
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4121..4147
                     /label=Bm5seqF
     primer_bind     complement(4194..4211)
                     /label=gvpL_rev
     misc_feature    4211^4212
                     /label=LS
     primer_bind     4212..4236
                     /label=gvpS_fwd
     misc_feature    4212..4220
                     /label=RBS
     CDS             4228..4515
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(4531..4550)
                     /label=gvpS_rev
     misc_feature    4550
                     /label=SK
     primer_bind     4551..4569
                     /label=gvpJ_fwd
     misc_feature    4551
                     /label=KJ
     misc_feature    4554..4562
                     /label=RBS
     CDS             4566..4868
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4691
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4697..4718
                     /label=Bm6seqF
     primer_bind     complement(4863..4884)
                     /label=gvpJ_rev
     misc_feature    4884^4885
                     /label=JT
     primer_bind     4885..4908
                     /label=gvpT_fwd
     misc_feature    4891..4899
                     /label=RBS
     CDS             4905..5783
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4926..4952
                     /label=Bm6.1seq
     misc_feature    5229
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5292
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5366..5392
                     /label=Bm7seqF
     primer_bind     complement(5771..5788)
                     /label=gvpT_rev
     misc_feature    5788^5789
                     /label=TU
     primer_bind     5789..5809
                     /label=gvpU_fwd
     misc_feature    5791..5799
                     /label=RBS
     CDS             5807..6223
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     6146..6170
                     /label=GvpUseqF
     misc_feature    6376^6377
                     /label=UV
     primer_bind     6377..6394
                     /label=vector_fwd
     terminator      6493..6540
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6607..6698
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6688..6693
                     /label=Emopec_63.3%
     CDS             6699..7559
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7633..8221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat tagatctaac tattggaggc tactaaaaat ggaaattaaa
     1141 aaaattatgc aagccgtgaa cgactttttc ggtgaacacg tagctcctcc tcataaaatt
     1201 acctcggtgg aagctactga agatgaaggt tggagagtta ttgttgaagt cattgaagaa
     1261 cgagaatata tgaaaaaata cgccaaagat gaaatgctcg gaacgtacga gtgctttgta
     1321 aataaagaaa aagaagtcat ttcattcaaa cgactcgacg tcagatatag aagcgccatt
     1381 ggcattgaag cataaacgat aagatggcag gaggaacgta aaaatgaccg tcttaacaga
     1441 caaaaggaaa aaaggcagtg gagcttttat acaagatgac gagacaaaag aggttctttc
     1501 aagagcgctg agctatttaa aatccggcta ttccattcat tttacaggtc ctgccggcgg
     1561 aggcaaaacc tctttagcgc gagcgcttgc taaaaagaga aagcgtcctg taatgctgat
     1621 gcacgggaat cacgagctca acaacaaaga tttaattggc gattttacgg gatacacgag
     1681 caaaaaagta atcgaccagt acgttcgttc tgtctataaa aaagatgaac aggtgagtga
     1741 aaactggcag gatggccgat tgcttgaagc tgtaaaaaat ggctatacgc tgatttacga
     1801 cgaatttact cgttctaagc ctgcgacgaa taatatcttt ctatcgatat tagaagaagg
     1861 cgtgctgccg ctgtatggag taaaaatgac cgatcctttt gtgcgcgtgc atcccgattt
     1921 ccgcgtcatc ttcacaagca atccagctga gtatgccggc gtatatgata cgcaagatgc
     1981 gcttctcgac aggttaatta ccatgtttat tgattataaa gacatcgaca gagagacagc
     2041 gattttaacg gagaaaacgg acgtagaaga agatgaagcg cgcacaattg taacgctcgt
     2101 agcaaacgtg cgaaaccgct ctggagacga aaacagcagc ggacttagcc tgcgggcttc
     2161 gcttatgatc gctacccttg ccacgcagca agacattcct atcgatggaa gtgacgaaga
     2221 ttttcaaacg ttatgtatcg atattttgca tcatccgctt accaaatgtt tggatgaaga
     2281 aaatgcaaaa agcaaagccg aaaaaatcat tttagaagaa tgtaagaata tagacactga
     2341 agaaaagtaa aggagcttga aaacatgagt gaaacaaacg aaacaggtat ttatattttt
     2401 agcgccattc aaacggataa agacgaagaa tttggcgccg tggaagtaga aggaacaaaa
     2461 gctgaaacat ttttgattcg ctacaaagac gcggctatgg tagcagctga agtaccgatg
     2521 aaaatttatc atcctaatcg ccaaaattta ttaatgcatc aaaacgcagt agcagcgatt
     2581 atggacaaga acgatacggt tattccaatc agctttggga atgtattcaa atcaaaagaa
     2641 gacgtaaaag ttcttttgga aaacctttat ccgcagtttg aaaagctgtt tccagcgatc
     2701 aaaggaaaaa ttgaagtcgg tttaaaagta attgggaaaa aagaatggct tgagaaaaaa
     2761 gtaaacgaaa atcctgaact tgagaaagta tcagcatccg taaaaggaaa atcagaagca
     2821 gccggttatt atgagcgtat tcaacttgga ggaatggctc aaaagatgtt tacttccctg
     2881 caaaaagaag tcaagacaga tgtgttttct ccgcttgaag aagcagcgga agcagcaaaa
     2941 gcaaatgagc caacgggcga aacgatgctt ttaaacgcgt ctttcttaat taaccgagaa
     3001 gatgaagcga agtttgatga aaaagtaaat gaagcgcatg aaaactggaa agacaaagcc
     3061 gattttcatt acagcggtcc ttggcctgct tataattttg tgaacattcg cctaaaagta
     3121 gaagagaaat aacgtgcttc acaaattagt aaccgcaccc attaaccttg tagtgaaaat
     3181 cggcgaaaaa gtacaggaag aagctgataa acagctatat gaccttccga cgattcagca
     3241 aaagctcatt cagcttcaaa tgatgtttga gcttggtgaa attccagaag aagcgtttca
     3301 agaaaaagaa gatgaattgt taatgaggta cgaaattgcg aaacgcagag aaattgaaca
     3361 atgggaagag ctaacacaaa aaagaaatga ggaatcctag atgggagaat tactgtattt
     3421 atacggttta attccaacaa aagaagcagc agccatagag ccgtttccat cttataaggg
     3481 gtttgacgga gaacattcac tgtacccaat tgcgtttgat caggtgacgg ctgtagtttc
     3541 taagctggat gctgacacct attcagaaaa agtgattcaa gaaaaaatgg agcaggatat
     3601 gagctggctg caggaaaaag catttcatca tcacgaaacg gtagccgctt tgtacgaaga
     3661 atttacgatc attccattaa aattttgcac catttataaa ggtgaagaaa gtctgcaggc
     3721 agctattgag attaacaaag aaaagataga gaattcactg acgctgcttc aaggaaatga
     3781 agagtggaat gtgaaaattt actgtgatga tacagagctt aaaaaaggaa tcagcgaaac
     3841 gaatgaaagc gtgaaagcga aaaaacaaga aattagtcac ttatcaccag gaagacagtt
     3901 ttttgaaaag aaaaaaatag atcagctgat tgaaaaagaa ttagagcttc acaaaaacaa
     3961 agtgtgtgaa gagatacatg acaagctaaa agaattatcg ctttatgact ctgttaaaaa
     4021 gaattggagc aaagacgtaa ctggcgcagc tgaacagatg gcgtggaaca gcgtgtttct
     4081 tctcccgtct ctgcagatta ctaagttcgt aaacgaaata gaagagcttc agcaaaggct
     4141 tgaaaataaa ggctggaagt ttgaagtgac gggaccatgg ccgccctatc atttctcgag
     4201 ctttgcgtaa agtgaggaat taacattatg tctcttaaac aatccatgga gaataaagat
     4261 attgctctta ttgatatttt agatgtcatt ttagataaag gagtcgccat taaaggagac
     4321 ttaatcattt ccatagctgg cgtcgattta gtgtatttgg atttgcgggt gcttatttct
     4381 tcggttgaaa cgcttgtgca agcaaaagaa ggaaatcaca aaccaatcac ttctgaacaa
     4441 tttgataaac aaaaggaaga gttaatggac gcgaccggcc agccgagcaa atggaccaat
     4501 ccgcttggct cctgaccctt gcgagcagtc gtatgctttc tcgaattccg agtaggagga
     4561 acagtatggc agtcgaacat aatatgcagt caagtacgat tgtagatgtg ctcgaaaaga
     4621 ttttggataa aggagtcgtt atagcggggg acatcaccgt aggaattgca gatgtcgagc
     4681 tattaacgat aaagatccgc ttgattgtgg cttcggttga taaggcaaaa gaaatcggca
     4741 tggactggtg ggaaaatgat ccgtatctca gttcaaaagg agccaataac aaagcgctcg
     4801 aagaagaaaa taaaatgctg catgagcggt taaaaacgct tgaagaaaaa atagaaacga
     4861 aacgttaaaa actgtacgct acttaaaaaa tggagggatt tacaatggca actgaaacaa
     4921 aattagataa cacacaggca gaaaacaagg aaaataaaaa tgcggaaaac ggttcaaaag
     4981 aaaagaacgg ttcaaaagca agcaaaacaa caagcagcgg gccaatcaaa cgagcggtag
     5041 caggaggcat catcggtgca acgattggat atgtatcgac tcctgaaaat cgaaaaagtc
     5101 tccttgaccg cattgataca gacgaattaa aaagcaaagc atctgattta ggaacaaagg
     5161 taaaagaaaa atcaaaaagc agcgtggcca gcctgaaaac atctgcggga agcttgttta
     5221 aaaaagataa agataaatca aaagatgatg aagaaaacgt aaattcttct agtagcgaaa
     5281 cagaagacga taacgttcaa gagtacgacg agttaaaaga agaaaatcaa actcttcaag
     5341 atcgcttatc acagcttgaa gaaaaaatga acatgcttgt tgagcttagc ctcaataaaa
     5401 atcaagacga agaagcggaa gatacagatt ccgacgaaga agagaacgat gagaacgatg
     5461 aaaacgatga aaacgagcag gacgatgaaa acgaagaaga aacatctaag ccacgtaaaa
     5521 aggataaaaa agaagctgag gaagaagaaa gtgaaagtga cgaagacagc gaggaagaag
     5581 aggaagattc tcgctcaaac aaaaaaaata aaaaagtaaa aacagaagaa gaagacgaag
     5641 atgaaagcga agaagaaaaa aaggaagcga aaccaaaaaa gtcaacagct aaaaaatcaa
     5701 aaaatacaaa agcaaagaaa aacacggacg aagaagatga tgaagcaaca tctctttcta
     5761 gtgaagacga tacaacagcc taagacgtaa aggaggaaag aaagacatga gtacaggccc
     5821 ttctttttca actaaagaca atacgcttga atactttgtg aaagcttcta ataaacacgg
     5881 cttttcactt gatatttcat taaatgtaaa cggcgctgtg atttccggta ccatgatttc
     5941 agcaaaagaa tactttgatt acttaagcga aacgtttgaa gaaggcagtg aagtggctca
     6001 ggcgctaagc gaacaattct ctttagcaag cgaagcgagc gaatcaaacg gagaagcaga
     6061 agcccatttt attcatttga aaaatacaaa gatttactgt ggagacagta aatctactcc
     6121 ttctaaaggc aaaatctttt ggagagggaa aatagcagaa gtagacgggt ttttcttagg
     6181 aaagatttct gatgcaaaat caacgagtaa aaagagttca taaaaaacgg cggggtgatt
     6241 gccccgccgt tttttagtga tgtgatgaga tgtgcagctt cttttttcga tacatgagcg
     6301 gagtcatctt catttctttt cgaaactgat taataaaata gcttgtacta ttaaacccta
     6361 cttgataggc gacttccgtc acatttgctt ctgcttgctg cagtccggat tcgcgaacgc
     6421 gtctcgagag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     6481 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     6541 ctgaaaggag gaactatatc cgactagtag cccgcctaat gagcgggctt ttttttaatt
     6601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     6721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     6781 tcgtgaaagt aaaagacgca gaggaccaat tgggggcacg agtgggatac atagaactgg
     6841 acttgaatag cggtaaaatc cttgagagtt ttcgccctga agagcgtttt ccaatgatga
     6901 gcactttcaa agttctgcta tgtggagcag tattatcccg tgtagatgcg gggcaagagc
     6961 aactcggacg acgaatacac tattcgcaga atgacttggt tgaatactcc ccagtgacag
     7021 aaaagcacct tacggacgga atgacggtaa gagaattatg tagtgccgcc ataacgatga
     7081 gtgataacac tgcggcgaac ttacttctga caaccatcgg tggaccgaag gaattaaccg
     7141 cttttttgca caatatggga gaccatgtaa ctcgccttga ccgttgggaa ccagaactga
     7201 atgaagccat accaaacgac gagcgagaca ccacaatgcc tgcggcaatg gcaacaacat
     7261 tacgcaaact attaactggc gaactactta ctctggcttc acggcaacaa ttaatagact
     7321 ggcttgaagc ggataaagtt gcaggaccac tactgcgttc ggcacttcct gctggctggt
     7381 ttattgctga taaatctggg gcaggagagc gtggttcacg gggtatcatt gccgcacttg
     7441 gaccagatgg taagccttcc cgtatcgtag ttatctacac gacgggtagt caggcaacta
     7501 tggacgaacg aaatagacag attgctgaaa taggggcttc actgattaag cattggtaaa
     7561 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacggaccgg tagaaaagat
     7621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7741 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8221 a
//
