LOCUS       Exported                7809 bp ds-DNA     circular SYN 12-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100^1101
                     /label=VB
     misc_feature    1108..1116
                     /label=RBS
     CDS             1122..1388
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     primer_bind     1411..1435
                     /label=Bm1seq
     primer_bind     complement(1420..1435)
                     /label=gvpB_rev
     misc_feature    1435
                     /label=BR
     primer_bind     1436..1456
                     /label=gvpN_fwd
     misc_feature    1436
                     /label=RN
     misc_feature    1437..1445
                     /label=RBS
     CDS             1452..2378
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1482..1507
                     /label=Bm1.1seqF
     misc_feature    1514
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1884..1903
                     /label=Bm2seqF
     primer_bind     complement(2349..2378)
                     /label=gvpN_rev
     misc_feature    2378
                     /label=NF
     primer_bind     2379..2401
                     /label=gvpG_fwd
     CDS             2379..2393
                     /codon_start=1
                     /label=GvpF
                     /translation="VEEK"
     misc_feature    2379
                     /label=FG
     misc_feature    2383..2391
                     /label=RBS
     CDS             2395..2661
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     2607..2634
                     /label=Bm4seqF
     primer_bind     complement(2625..2646)
                     /label=gvpG_rev
     misc_feature    2646^2647
                     /label=GL
     primer_bind     2647..2666
                     /label=gvpL_fwd
     misc_feature    2648..2656
                     /label=RBS
     CDS             2662..3471
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    2732
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    2801
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3251
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3382..3408
                     /label=Bm5seqF
     primer_bind     complement(3455..3472)
                     /label=gvpL_rev
     misc_feature    3472^3473
                     /label=LS
     primer_bind     3473..3497
                     /label=gvpS_fwd
     misc_feature    3473..3481
                     /label=RBS
     CDS             3489..3776
                     /codon_start=1
                     /label=GvpS
                     /translation="MSLKQSMENKDIALIDILDVILDKGVAIKGDLIISIAGVDLVYLD
                     LRVLISSVETLVQAKEGNHKPITSEQFDKQKEELMDATGQPSKWTNPLGS"
     primer_bind     complement(3792..3811)
                     /label=gvpS_rev
     misc_feature    3811^3812
                     /label=SK
     primer_bind     3812..3827
                     /label=gvpK_fwd
     misc_feature    3833..3841
                     /label=RBS
     CDS             3848..4132
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4123..4138)
                     /label=gvpK_rev
     misc_feature    4138^4139
                     /label=KJ
     primer_bind     4139..4157
                     /label=gvpJ_fwd
     misc_feature    4142..4150
                     /label=RBS
     CDS             4154..4456
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4279
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4285..4306
                     /label=Bm6seqF
     primer_bind     complement(4451..4472)
                     /label=gvpJ_rev
     misc_feature    4472^4473
                     /label=JT
     primer_bind     4473..4496
                     /label=gvpT_fwd
     misc_feature    4479..4487
                     /label=RBS
     CDS             4493..5371
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4514..4540
                     /label=Bm6.1seq
     misc_feature    4817
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4880
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4954..4980
                     /label=Bm7seqF
     primer_bind     complement(5359..5376)
                     /label=gvpT_rev
     misc_feature    5376^5377
                     /label=TU
     primer_bind     5377..5397
                     /label=gvpU_fwd
     misc_feature    5379..5387
                     /label=RBS
     CDS             5395..5811
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     5734..5758
                     /label=GvpUseqF
     misc_feature    5964^5965
                     /label=UV
     primer_bind     5965..5982
                     /label=vector_fwd
     terminator      6081..6128
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6195..6286
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6276..6281
                     /label=Emopec_63.3%
     CDS             6287..7147
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7221..7809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat aagcgaaagg agaaatgaga tatgtctatt caaaaaagta
     1141 ctaatagttc aagtttagca gaagtcattg accgtatttt agataaagga attgttattg
     1201 atgcttttgc aagagtttct gttgtaggaa ttgaaatttt aacgattgaa gcgcgagtgg
     1261 ttattgccag tgttgataca tggttacgct atgcagaagc agtagggctt cttcgtgacg
     1321 acgtagaaga aaacggtctt cctgaacgtt caaattcaag tgaagggcag ccgcgtttta
     1381 gtatttaata tgaagtggta tggaaccttc cttttttgag ggtggttcca gctttcagga
     1441 ggaacgtaaa aatgaccgtc ttaacagaca aaaggaaaaa aggcagtgga gcttttatac
     1501 aagatgacga gacaaaagag gttctttcaa gagcgctgag ctatttaaaa tccggctatt
     1561 ccattcattt tacaggtcct gccggcggag gcaaaacctc tttagcgcga gcgcttgcta
     1621 aaaagagaaa gcgtcctgta atgctgatgc acgggaatca cgagctcaac aacaaagatt
     1681 taattggcga ttttacggga tacacgagca aaaaagtaat cgaccagtac gttcgttctg
     1741 tctataaaaa agatgaacag gtgagtgaaa actggcagga tggccgattg cttgaagctg
     1801 taaaaaatgg ctatacgctg atttacgacg aatttactcg ttctaagcct gcgacgaata
     1861 atatctttct atcgatatta gaagaaggcg tgctgccgct gtatggagta aaaatgaccg
     1921 atccttttgt gcgcgtgcat cccgatttcc gcgtcatctt cacaagcaat ccagctgagt
     1981 atgccggcgt atatgatacg caagatgcgc ttctcgacag gttaattacc atgtttattg
     2041 attataaaga catcgacaga gagacagcga ttttaacgga gaaaacggac gtagaagaag
     2101 atgaagcgcg cacaattgta acgctcgtag caaacgtgcg aaaccgctct ggagacgaaa
     2161 acagcagcgg acttagcctg cgggcttcgc ttatgatcgc tacccttgcc acgcagcaag
     2221 acattcctat cgatggaagt gacgaagatt ttcaaacgtt atgtatcgat attttgcatc
     2281 atccgcttac caaatgtttg gatgaagaaa atgcaaaaag caaagccgaa aaaatcattt
     2341 tagaagaatg taagaatata gacactgaag aaaagtaagt agaagagaaa taacgtgctt
     2401 cacaaattag taaccgcacc cattaacctt gtagtgaaaa tcggcgaaaa agtacaggaa
     2461 gaagctgata aacagctata tgaccttccg acgattcagc aaaagctcat tcagcttcaa
     2521 atgatgtttg agcttggtga aattccagaa gaagcgtttc aagaaaaaga agatgaattg
     2581 ttaatgaggt acgaaattgc gaaacgcaga gaaattgaac aatgggaaga gctaacacaa
     2641 aaaagaaatg aggaatccta gatgggagaa ttactgtatt tatacggttt aattccaaca
     2701 aaagaagcag cagccataga gccgtttcca tcttataagg ggtttgacgg agaacattca
     2761 ctgtacccaa ttgcgtttga tcaggtgacg gctgtagttt ctaagctgga tgctgacacc
     2821 tattcagaaa aagtgattca agaaaaaatg gagcaggata tgagctggct gcaggaaaaa
     2881 gcatttcatc atcacgaaac ggtagccgct ttgtacgaag aatttacgat cattccatta
     2941 aaattttgca ccatttataa aggtgaagaa agtctgcagg cagctattga gattaacaaa
     3001 gaaaagatag agaattcact gacgctgctt caaggaaatg aagagtggaa tgtgaaaatt
     3061 tactgtgatg atacagagct taaaaaagga atcagcgaaa cgaatgaaag cgtgaaagcg
     3121 aaaaaacaag aaattagtca cttatcacca ggaagacagt tttttgaaaa gaaaaaaata
     3181 gatcagctga ttgaaaaaga attagagctt cacaaaaaca aagtgtgtga agagatacat
     3241 gacaagctaa aagaattatc gctttatgac tctgttaaaa agaattggag caaagacgta
     3301 actggcgcag ctgaacagat ggcgtggaac agcgtgtttc ttctcccgtc tctgcagatt
     3361 actaagttcg taaacgaaat agaagagctt cagcaaaggc ttgaaaataa aggctggaag
     3421 tttgaagtga cgggaccatg gccgccctat catttctcga gctttgcgta aagtgaggaa
     3481 ttaacattat gtctcttaaa caatccatgg agaataaaga tattgctctt attgatattt
     3541 tagatgtcat tttagataaa ggagtcgcca ttaaaggaga cttaatcatt tccatagctg
     3601 gcgtcgattt agtgtatttg gatttgcggg tgcttatttc ttcggttgaa acgcttgtgc
     3661 aagcaaaaga aggaaatcac aaaccaatca cttctgaaca atttgataaa caaaaggaag
     3721 agttaatgga cgcgaccggc cagccgagca aatggaccaa tccgcttggc tcctgaccct
     3781 tgcgagcagt cgtatgcttt ctcgaattcc gagcggttaa gcgtgacagt ccaggaggaa
     3841 ttaatggatg caaccggtca gccaagcaaa tggacgaatc cacttggatc ctgatcaagc
     3901 tgaacaaggc ttagcgcagc ttgtgatgac agttattgag ctattgaggc aaatcgttga
     3961 acgtcatgcc atgaggcggg tggagggtgg aacgttgacg gacgaacaaa ttgaaaactt
     4021 aggaattgca ctaatgaact tagaagaaaa aatggacgag ttgaaagagg tgttcggtct
     4081 ggatgcagaa gatttaaata ttgatcttgg accgctaggc agcctgcttt aagcggtcag
     4141 taggaggaac agtatggcag tcgaacataa tatgcagtca agtacgattg tagatgtgct
     4201 cgaaaagatt ttggataaag gagtcgttat agcgggggac atcaccgtag gaattgcaga
     4261 tgtcgagcta ttaacgataa agatccgctt gattgtggct tcggttgata aggcaaaaga
     4321 aatcggcatg gactggtggg aaaatgatcc gtatctcagt tcaaaaggag ccaataacaa
     4381 agcgctcgaa gaagaaaata aaatgctgca tgagcggtta aaaacgcttg aagaaaaaat
     4441 agaaacgaaa cgttaaaaac tgtacgctac ttaaaaaatg gagggattta caatggcaac
     4501 tgaaacaaaa ttagataaca cacaggcaga aaacaaggaa aataaaaatg cggaaaacgg
     4561 ttcaaaagaa aagaacggtt caaaagcaag caaaacaaca agcagcgggc caatcaaacg
     4621 agcggtagca ggaggcatca tcggtgcaac gattggatat gtatcgactc ctgaaaatcg
     4681 aaaaagtctc cttgaccgca ttgatacaga cgaattaaaa agcaaagcat ctgatttagg
     4741 aacaaaggta aaagaaaaat caaaaagcag cgtggccagc ctgaaaacat ctgcgggaag
     4801 cttgtttaaa aaagataaag ataaatcaaa agatgatgaa gaaaacgtaa attcttctag
     4861 tagcgaaaca gaagacgata acgttcaaga gtacgacgag ttaaaagaag aaaatcaaac
     4921 tcttcaagat cgcttatcac agcttgaaga aaaaatgaac atgcttgttg agcttagcct
     4981 caataaaaat caagacgaag aagcggaaga tacagattcc gacgaagaag agaacgatga
     5041 gaacgatgaa aacgatgaaa acgagcagga cgatgaaaac gaagaagaaa catctaagcc
     5101 acgtaaaaag gataaaaaag aagctgagga agaagaaagt gaaagtgacg aagacagcga
     5161 ggaagaagag gaagattctc gctcaaacaa aaaaaataaa aaagtaaaaa cagaagaaga
     5221 agacgaagat gaaagcgaag aagaaaaaaa ggaagcgaaa ccaaaaaagt caacagctaa
     5281 aaaatcaaaa aatacaaaag caaagaaaaa cacggacgaa gaagatgatg aagcaacatc
     5341 tctttctagt gaagacgata caacagccta agacgtaaag gaggaaagaa agacatgagt
     5401 acaggccctt ctttttcaac taaagacaat acgcttgaat actttgtgaa agcttctaat
     5461 aaacacggct tttcacttga tatttcatta aatgtaaacg gcgctgtgat ttccggtacc
     5521 atgatttcag caaaagaata ctttgattac ttaagcgaaa cgtttgaaga aggcagtgaa
     5581 gtggctcagg cgctaagcga acaattctct ttagcaagcg aagcgagcga atcaaacgga
     5641 gaagcagaag cccattttat tcatttgaaa aatacaaaga tttactgtgg agacagtaaa
     5701 tctactcctt ctaaaggcaa aatcttttgg agagggaaaa tagcagaagt agacgggttt
     5761 ttcttaggaa agatttctga tgcaaaatca acgagtaaaa agagttcata aaaaacggcg
     5821 gggtgattgc cccgccgttt tttagtgatg tgatgagatg tgcagcttct tttttcgata
     5881 catgagcgga gtcatcttca tttcttttcg aaactgatta ataaaatagc ttgtactatt
     5941 aaaccctact tgataggcga cttccgtcac atttgcttct gcttgctgca gtccggattc
     6001 gcgaacgcgt ctcgagagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     6061 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     6121 gttttttgct gaaaggagga actatatccg actagtagcc cgcctaatga gcgggctttt
     6181 ttttaattcc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6241 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6301 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6361 agaaacgctc gtgaaagtaa aagacgcaga ggaccaattg ggggcacgag tgggatacat
     6421 agaactggac ttgaatagcg gtaaaatcct tgagagtttt cgccctgaag agcgttttcc
     6481 aatgatgagc actttcaaag ttctgctatg tggagcagta ttatcccgtg tagatgcggg
     6541 gcaagagcaa ctcggacgac gaatacacta ttcgcagaat gacttggttg aatactcccc
     6601 agtgacagaa aagcacctta cggacggaat gacggtaaga gaattatgta gtgccgccat
     6661 aacgatgagt gataacactg cggcgaactt acttctgaca accatcggtg gaccgaagga
     6721 attaaccgct tttttgcaca atatgggaga ccatgtaact cgccttgacc gttgggaacc
     6781 agaactgaat gaagccatac caaacgacga gcgagacacc acaatgcctg cggcaatggc
     6841 aacaacatta cgcaaactat taactggcga actacttact ctggcttcac ggcaacaatt
     6901 aatagactgg cttgaagcgg ataaagttgc aggaccacta ctgcgttcgg cacttcctgc
     6961 tggctggttt attgctgata aatctggggc aggagagcgt ggttcacggg gtatcattgc
     7021 cgcacttgga ccagatggta agccttcccg tatcgtagtt atctacacga cgggtagtca
     7081 ggcaactatg gacgaacgaa atagacagat tgctgaaata ggggcttcac tgattaagca
     7141 ttggtaaacc gatacaatta aaggctcctt ttggagcctt tttttttgga cggaccggta
     7201 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7261 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7321 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7381 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7441 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7501 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7561 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7621 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7681 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7741 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7801 ctatggaaa
//
