LOCUS       Exported                8237 bp ds-DNA     circular SYN 26-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8237
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100^1101
                     /label=VB
     misc_feature    1108..1116
                     /label=RBS
     CDS             1122..1388
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     primer_bind     1411..1435
                     /label=Bm1seq
     primer_bind     complement(1420..1435)
                     /label=gvpB_rev
     misc_feature    1435
                     /label=BR
     primer_bind     1436..1456
                     /label=gvpN_fwd
     misc_feature    1436
                     /label=RN
     misc_feature    1437..1445
                     /label=RBS
     CDS             1452..2378
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1482..1507
                     /label=Bm1.1seqF
     misc_feature    1514
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1884..1903
                     /label=Bm2seqF
     primer_bind     complement(2349..2378)
                     /label=gvpN_rev
     misc_feature    2378^2379
                     /label=NF
     primer_bind     2379..2398
                     /label=gvpF_fwd
     misc_feature    2379..2387
                     /label=RBS
     CDS             2393..3160
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2626..2653
                     /label=Bm3seqF
     primer_bind     complement(3125..3145)
                     /label=gvpF_rev
     misc_feature    3145^3146
                     /label=FG
     primer_bind     3146..3168
                     /label=gvpG_fwd
     misc_feature    3150..3158
                     /label=RBS
     CDS             3162..3428
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3374..3401
                     /label=Bm4seqF
     primer_bind     complement(3392..3413)
                     /label=gvpG_rev
     misc_feature    3413^3414
                     /label=GL
     primer_bind     3414..3433
                     /label=gvpL_fwd
     misc_feature    3415..3423
                     /label=RBS
     CDS             3429..4238
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3499
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3568
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4018
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4149..4175
                     /label=Bm5seqF
     primer_bind     complement(4222..4239)
                     /label=gvpL_rev
     misc_feature    4239
                     /label=LS
     primer_bind     4240..4255
                     /label=gvpK_fwd
     misc_feature    4240
                     /label=SK
     misc_feature    4261..4269
                     /label=RBS
     CDS             4276..4560
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4551..4566)
                     /label=gvpK_rev
     misc_feature    4566^4567
                     /label=KJ
     primer_bind     4567..4585
                     /label=gvpJ_fwd
     misc_feature    4570..4578
                     /label=RBS
     CDS             4582..4884
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4707
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4713..4734
                     /label=Bm6seqF
     primer_bind     complement(4879..4900)
                     /label=gvpJ_rev
     misc_feature    4900^4901
                     /label=JT
     primer_bind     4901..4924
                     /label=gvpT_fwd
     misc_feature    4907..4915
                     /label=RBS
     CDS             4921..5799
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4942..4968
                     /label=Bm6.1seq
     misc_feature    5245
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5308
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5382..5408
                     /label=Bm7seqF
     primer_bind     complement(5787..5804)
                     /label=gvpT_rev
     misc_feature    5804^5805
                     /label=TU
     primer_bind     5805..5825
                     /label=gvpU_fwd
     misc_feature    5807..5815
                     /label=RBS
     CDS             5823..6239
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     6162..6186
                     /label=GvpUseqF
     misc_feature    6392^6393
                     /label=UV
     primer_bind     6393..6410
                     /label=vector_fwd
     terminator      6509..6556
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6623..6714
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6704..6709
                     /label=Emopec_63.3%
     CDS             6715..7575
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7649..8237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat aagcgaaagg agaaatgaga tatgtctatt caaaaaagta
     1141 ctaatagttc aagtttagca gaagtcattg accgtatttt agataaagga attgttattg
     1201 atgcttttgc aagagtttct gttgtaggaa ttgaaatttt aacgattgaa gcgcgagtgg
     1261 ttattgccag tgttgataca tggttacgct atgcagaagc agtagggctt cttcgtgacg
     1321 acgtagaaga aaacggtctt cctgaacgtt caaattcaag tgaagggcag ccgcgtttta
     1381 gtatttaata tgaagtggta tggaaccttc cttttttgag ggtggttcca gctttcagga
     1441 ggaacgtaaa aatgaccgtc ttaacagaca aaaggaaaaa aggcagtgga gcttttatac
     1501 aagatgacga gacaaaagag gttctttcaa gagcgctgag ctatttaaaa tccggctatt
     1561 ccattcattt tacaggtcct gccggcggag gcaaaacctc tttagcgcga gcgcttgcta
     1621 aaaagagaaa gcgtcctgta atgctgatgc acgggaatca cgagctcaac aacaaagatt
     1681 taattggcga ttttacggga tacacgagca aaaaagtaat cgaccagtac gttcgttctg
     1741 tctataaaaa agatgaacag gtgagtgaaa actggcagga tggccgattg cttgaagctg
     1801 taaaaaatgg ctatacgctg atttacgacg aatttactcg ttctaagcct gcgacgaata
     1861 atatctttct atcgatatta gaagaaggcg tgctgccgct gtatggagta aaaatgaccg
     1921 atccttttgt gcgcgtgcat cccgatttcc gcgtcatctt cacaagcaat ccagctgagt
     1981 atgccggcgt atatgatacg caagatgcgc ttctcgacag gttaattacc atgtttattg
     2041 attataaaga catcgacaga gagacagcga ttttaacgga gaaaacggac gtagaagaag
     2101 atgaagcgcg cacaattgta acgctcgtag caaacgtgcg aaaccgctct ggagacgaaa
     2161 acagcagcgg acttagcctg cgggcttcgc ttatgatcgc tacccttgcc acgcagcaag
     2221 acattcctat cgatggaagt gacgaagatt ttcaaacgtt atgtatcgat attttgcatc
     2281 atccgcttac caaatgtttg gatgaagaaa atgcaaaaag caaagccgaa aaaatcattt
     2341 tagaagaatg taagaatata gacactgaag aaaagtaaag gagcttgaaa acatgagtga
     2401 aacaaacgaa acaggtattt atatttttag cgccattcaa acggataaag acgaagaatt
     2461 tggcgccgtg gaagtagaag gaacaaaagc tgaaacattt ttgattcgct acaaagacgc
     2521 ggctatggta gcagctgaag taccgatgaa aatttatcat cctaatcgcc aaaatttatt
     2581 aatgcatcaa aacgcagtag cagcgattat ggacaagaac gatacggtta ttccaatcag
     2641 ctttgggaat gtattcaaat caaaagaaga cgtaaaagtt cttttggaaa acctttatcc
     2701 gcagtttgaa aagctgtttc cagcgatcaa aggaaaaatt gaagtcggtt taaaagtaat
     2761 tgggaaaaaa gaatggcttg agaaaaaagt aaacgaaaat cctgaacttg agaaagtatc
     2821 agcatccgta aaaggaaaat cagaagcagc cggttattat gagcgtattc aacttggagg
     2881 aatggctcaa aagatgttta cttccctgca aaaagaagtc aagacagatg tgttttctcc
     2941 gcttgaagaa gcagcggaag cagcaaaagc aaatgagcca acgggcgaaa cgatgctttt
     3001 aaacgcgtct ttcttaatta accgagaaga tgaagcgaag tttgatgaaa aagtaaatga
     3061 agcgcatgaa aactggaaag acaaagccga ttttcattac agcggtcctt ggcctgctta
     3121 taattttgtg aacattcgcc taaaagtaga agagaaataa cgtgcttcac aaattagtaa
     3181 ccgcacccat taaccttgta gtgaaaatcg gcgaaaaagt acaggaagaa gctgataaac
     3241 agctatatga ccttccgacg attcagcaaa agctcattca gcttcaaatg atgtttgagc
     3301 ttggtgaaat tccagaagaa gcgtttcaag aaaaagaaga tgaattgtta atgaggtacg
     3361 aaattgcgaa acgcagagaa attgaacaat gggaagagct aacacaaaaa agaaatgagg
     3421 aatcctagat gggagaatta ctgtatttat acggtttaat tccaacaaaa gaagcagcag
     3481 ccatagagcc gtttccatct tataaggggt ttgacggaga acattcactg tacccaattg
     3541 cgtttgatca ggtgacggct gtagtttcta agctggatgc tgacacctat tcagaaaaag
     3601 tgattcaaga aaaaatggag caggatatga gctggctgca ggaaaaagca tttcatcatc
     3661 acgaaacggt agccgctttg tacgaagaat ttacgatcat tccattaaaa ttttgcacca
     3721 tttataaagg tgaagaaagt ctgcaggcag ctattgagat taacaaagaa aagatagaga
     3781 attcactgac gctgcttcaa ggaaatgaag agtggaatgt gaaaatttac tgtgatgata
     3841 cagagcttaa aaaaggaatc agcgaaacga atgaaagcgt gaaagcgaaa aaacaagaaa
     3901 ttagtcactt atcaccagga agacagtttt ttgaaaagaa aaaaatagat cagctgattg
     3961 aaaaagaatt agagcttcac aaaaacaaag tgtgtgaaga gatacatgac aagctaaaag
     4021 aattatcgct ttatgactct gttaaaaaga attggagcaa agacgtaact ggcgcagctg
     4081 aacagatggc gtggaacagc gtgtttcttc tcccgtctct gcagattact aagttcgtaa
     4141 acgaaataga agagcttcag caaaggcttg aaaataaagg ctggaagttt gaagtgacgg
     4201 gaccatggcc gccctatcat ttctcgagct ttgcgtaaaa gcggttaagc gtgacagtcc
     4261 aggaggaatt aatggatgca accggtcagc caagcaaatg gacgaatcca cttggatcct
     4321 gatcaagctg aacaaggctt agcgcagctt gtgatgacag ttattgagct attgaggcaa
     4381 atcgttgaac gtcatgccat gaggcgggtg gagggtggaa cgttgacgga cgaacaaatt
     4441 gaaaacttag gaattgcact aatgaactta gaagaaaaaa tggacgagtt gaaagaggtg
     4501 ttcggtctgg atgcagaaga tttaaatatt gatcttggac cgctaggcag cctgctttaa
     4561 gcggtcagta ggaggaacag tatggcagtc gaacataata tgcagtcaag tacgattgta
     4621 gatgtgctcg aaaagatttt ggataaagga gtcgttatag cgggggacat caccgtagga
     4681 attgcagatg tcgagctatt aacgataaag atccgcttga ttgtggcttc ggttgataag
     4741 gcaaaagaaa tcggcatgga ctggtgggaa aatgatccgt atctcagttc aaaaggagcc
     4801 aataacaaag cgctcgaaga agaaaataaa atgctgcatg agcggttaaa aacgcttgaa
     4861 gaaaaaatag aaacgaaacg ttaaaaactg tacgctactt aaaaaatgga gggatttaca
     4921 atggcaactg aaacaaaatt agataacaca caggcagaaa acaaggaaaa taaaaatgcg
     4981 gaaaacggtt caaaagaaaa gaacggttca aaagcaagca aaacaacaag cagcgggcca
     5041 atcaaacgag cggtagcagg aggcatcatc ggtgcaacga ttggatatgt atcgactcct
     5101 gaaaatcgaa aaagtctcct tgaccgcatt gatacagacg aattaaaaag caaagcatct
     5161 gatttaggaa caaaggtaaa agaaaaatca aaaagcagcg tggccagcct gaaaacatct
     5221 gcgggaagct tgtttaaaaa agataaagat aaatcaaaag atgatgaaga aaacgtaaat
     5281 tcttctagta gcgaaacaga agacgataac gttcaagagt acgacgagtt aaaagaagaa
     5341 aatcaaactc ttcaagatcg cttatcacag cttgaagaaa aaatgaacat gcttgttgag
     5401 cttagcctca ataaaaatca agacgaagaa gcggaagata cagattccga cgaagaagag
     5461 aacgatgaga acgatgaaaa cgatgaaaac gagcaggacg atgaaaacga agaagaaaca
     5521 tctaagccac gtaaaaagga taaaaaagaa gctgaggaag aagaaagtga aagtgacgaa
     5581 gacagcgagg aagaagagga agattctcgc tcaaacaaaa aaaataaaaa agtaaaaaca
     5641 gaagaagaag acgaagatga aagcgaagaa gaaaaaaagg aagcgaaacc aaaaaagtca
     5701 acagctaaaa aatcaaaaaa tacaaaagca aagaaaaaca cggacgaaga agatgatgaa
     5761 gcaacatctc tttctagtga agacgataca acagcctaag acgtaaagga ggaaagaaag
     5821 acatgagtac aggcccttct ttttcaacta aagacaatac gcttgaatac tttgtgaaag
     5881 cttctaataa acacggcttt tcacttgata tttcattaaa tgtaaacggc gctgtgattt
     5941 ccggtaccat gatttcagca aaagaatact ttgattactt aagcgaaacg tttgaagaag
     6001 gcagtgaagt ggctcaggcg ctaagcgaac aattctcttt agcaagcgaa gcgagcgaat
     6061 caaacggaga agcagaagcc cattttattc atttgaaaaa tacaaagatt tactgtggag
     6121 acagtaaatc tactccttct aaaggcaaaa tcttttggag agggaaaata gcagaagtag
     6181 acgggttttt cttaggaaag atttctgatg caaaatcaac gagtaaaaag agttcataaa
     6241 aaacggcggg gtgattgccc cgccgttttt tagtgatgtg atgagatgtg cagcttcttt
     6301 tttcgataca tgagcggagt catcttcatt tcttttcgaa actgattaat aaaatagctt
     6361 gtactattaa accctacttg ataggcgact tccgtcacat ttgcttctgc ttgctgcagt
     6421 ccggattcgc gaacgcgtct cgagagatcc ggctgctaac aaagcccgaa aggaagctga
     6481 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
     6541 cttgaggggt tttttgctga aaggaggaac tatatccgac tagtagcccg cctaatgagc
     6601 gggctttttt ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6781 gctcacccag aaacgctcgt gaaagtaaaa gacgcagagg accaattggg ggcacgagtg
     6841 ggatacatag aactggactt gaatagcggt aaaatccttg agagttttcg ccctgaagag
     6901 cgttttccaa tgatgagcac tttcaaagtt ctgctatgtg gagcagtatt atcccgtgta
     6961 gatgcggggc aagagcaact cggacgacga atacactatt cgcagaatga cttggttgaa
     7021 tactccccag tgacagaaaa gcaccttacg gacggaatga cggtaagaga attatgtagt
     7081 gccgccataa cgatgagtga taacactgcg gcgaacttac ttctgacaac catcggtgga
     7141 ccgaaggaat taaccgcttt tttgcacaat atgggagacc atgtaactcg ccttgaccgt
     7201 tgggaaccag aactgaatga agccatacca aacgacgagc gagacaccac aatgcctgcg
     7261 gcaatggcaa caacattacg caaactatta actggcgaac tacttactct ggcttcacgg
     7321 caacaattaa tagactggct tgaagcggat aaagttgcag gaccactact gcgttcggca
     7381 cttcctgctg gctggtttat tgctgataaa tctggggcag gagagcgtgg ttcacggggt
     7441 atcattgccg cacttggacc agatggtaag ccttcccgta tcgtagttat ctacacgacg
     7501 ggtagtcagg caactatgga cgaacgaaat agacagattg ctgaaatagg ggcttcactg
     7561 attaagcatt ggtaaaccga tacaattaaa ggctcctttt ggagcctttt tttttggacg
     7621 gaccggtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7681 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7741 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     7801 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7861 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7921 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7981 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8041 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8101 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8161 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8221 ggcggagcct atggaaa
//
