LOCUS       Exported                8290 bp ds-DNA     circular SYN 27-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100^1101
                     /label=VB
     misc_feature    1108..1116
                     /label=RBS
     CDS             1122..1388
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     primer_bind     1411..1436
                     /label=Bm1seq
     primer_bind     complement(1420..1435)
                     /label=gvpB_rev
     misc_feature    1435^1436
                     /label=BR
     primer_bind     1436..1458
                     /label=gvpR_fwd
     misc_feature    1449..1457
                     /label=RBS
     CDS             1464..1730
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1721..1742)
                     /label=gvpR_rev
     misc_feature    1742^1743
                     /label=RN
     primer_bind     1743..1763
                     /label=gvpN_fwd
     misc_feature    1744..1752
                     /label=RBS
     CDS             1759..2685
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1789..1814
                     /label=Bm1.1seqF
     misc_feature    1821
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     2191..2210
                     /label=Bm2seqF
     primer_bind     complement(2656..2685)
                     /label=gvpN_rev
     misc_feature    2685^2686
                     /label=NF
     primer_bind     2686..2705
                     /label=gvpF_fwd
     misc_feature    2686..2694
                     /label=RBS
     CDS             2700..3452
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLK"
     primer_bind     2933..2960
                     /label=Bm3seqF
     primer_bind     complement(3432..3452)
                     /label=gvpF_rev
     primer_bind     complement(3435..3465)
                     /label=FL.fix_rev
     misc_feature    3456..3460
                     /label=RBS
     CDS             3456..3460
                     /codon_start=1
                     /label=GvpG
                     /translation="E"
     primer_bind     3466..3495
                     /label=FL.fix_fwd
     CDS             3475..3481
                     /codon_start=1
                     /label=GvpG
                     /translation="S"
     misc_feature    3475..3476
                     /label=RBS
     CDS             3482..4291
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3552
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3621
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4071
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4202..4228
                     /label=Bm5seqF
     primer_bind     complement(4275..4292)
                     /label=gvpL_rev
     misc_feature    4292
                     /label=LS
     primer_bind     4293..4308
                     /label=gvpK_fwd
     misc_feature    4293
                     /label=SK
     misc_feature    4314..4322
                     /label=RBS
     CDS             4329..4613
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4604..4619)
                     /label=gvpK_rev
     misc_feature    4619^4620
                     /label=KJ
     primer_bind     4620..4638
                     /label=gvpJ_fwd
     misc_feature    4623..4631
                     /label=RBS
     CDS             4635..4937
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4760
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4766..4787
                     /label=Bm6seqF
     primer_bind     complement(4932..4953)
                     /label=gvpJ_rev
     misc_feature    4953^4954
                     /label=JT
     primer_bind     4954..4977
                     /label=gvpT_fwd
     misc_feature    4960..4968
                     /label=RBS
     CDS             4974..5852
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4995..5021
                     /label=Bm6.1seq
     misc_feature    5298
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5361
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5435..5461
                     /label=Bm7seqF
     primer_bind     complement(5840..5857)
                     /label=gvpT_rev
     misc_feature    5857^5858
                     /label=TU
     primer_bind     5858..5878
                     /label=gvpU_fwd
     misc_feature    5860..5868
                     /label=RBS
     CDS             5876..6292
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     6215..6239
                     /label=GvpUseqF
     misc_feature    6445^6446
                     /label=UV
     primer_bind     6446..6463
                     /label=vector_fwd
     terminator      6562..6609
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6676..6767
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6757..6762
                     /label=Emopec_63.3%
     CDS             6768..7628
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7702..8290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat aagcgaaagg agaaatgaga tatgtctatt caaaaaagta
     1141 ctaatagttc aagtttagca gaagtcattg accgtatttt agataaagga attgttattg
     1201 atgcttttgc aagagtttct gttgtaggaa ttgaaatttt aacgattgaa gcgcgagtgg
     1261 ttattgccag tgttgataca tggttacgct atgcagaagc agtagggctt cttcgtgacg
     1321 acgtagaaga aaacggtctt cctgaacgtt caaattcaag tgaagggcag ccgcgtttta
     1381 gtatttaata tgaagtggta tggaaccttc cttttttgag ggtggttcca gcttttagat
     1441 ctaactattg gaggctacta aaaatggaaa ttaaaaaaat tatgcaagcc gtgaacgact
     1501 ttttcggtga acacgtagct cctcctcata aaattacctc ggtggaagct actgaagatg
     1561 aaggttggag agttattgtt gaagtcattg aagaacgaga atatatgaaa aaatacgcca
     1621 aagatgaaat gctcggaacg tacgagtgct ttgtaaataa agaaaaagaa gtcatttcat
     1681 tcaaacgact cgacgtcaga tatagaagcg ccattggcat tgaagcataa acgataagat
     1741 ggcaggagga acgtaaaaat gaccgtctta acagacaaaa ggaaaaaagg cagtggagct
     1801 tttatacaag atgacgagac aaaagaggtt ctttcaagag cgctgagcta tttaaaatcc
     1861 ggctattcca ttcattttac aggtcctgcc ggcggaggca aaacctcttt agcgcgagcg
     1921 cttgctaaaa agagaaagcg tcctgtaatg ctgatgcacg ggaatcacga gctcaacaac
     1981 aaagatttaa ttggcgattt tacgggatac acgagcaaaa aagtaatcga ccagtacgtt
     2041 cgttctgtct ataaaaaaga tgaacaggtg agtgaaaact ggcaggatgg ccgattgctt
     2101 gaagctgtaa aaaatggcta tacgctgatt tacgacgaat ttactcgttc taagcctgcg
     2161 acgaataata tctttctatc gatattagaa gaaggcgtgc tgccgctgta tggagtaaaa
     2221 atgaccgatc cttttgtgcg cgtgcatccc gatttccgcg tcatcttcac aagcaatcca
     2281 gctgagtatg ccggcgtata tgatacgcaa gatgcgcttc tcgacaggtt aattaccatg
     2341 tttattgatt ataaagacat cgacagagag acagcgattt taacggagaa aacggacgta
     2401 gaagaagatg aagcgcgcac aattgtaacg ctcgtagcaa acgtgcgaaa ccgctctgga
     2461 gacgaaaaca gcagcggact tagcctgcgg gcttcgctta tgatcgctac ccttgccacg
     2521 cagcaagaca ttcctatcga tggaagtgac gaagattttc aaacgttatg tatcgatatt
     2581 ttgcatcatc cgcttaccaa atgtttggat gaagaaaatg caaaaagcaa agccgaaaaa
     2641 atcattttag aagaatgtaa gaatatagac actgaagaaa agtaaaggag cttgaaaaca
     2701 tgagtgaaac aaacgaaaca ggtatttata tttttagcgc cattcaaacg gataaagacg
     2761 aagaatttgg cgccgtggaa gtagaaggaa caaaagctga aacatttttg attcgctaca
     2821 aagacgcggc tatggtagca gctgaagtac cgatgaaaat ttatcatcct aatcgccaaa
     2881 atttattaat gcatcaaaac gcagtagcag cgattatgga caagaacgat acggttattc
     2941 caatcagctt tgggaatgta ttcaaatcaa aagaagacgt aaaagttctt ttggaaaacc
     3001 tttatccgca gtttgaaaag ctgtttccag cgatcaaagg aaaaattgaa gtcggtttaa
     3061 aagtaattgg gaaaaaagaa tggcttgaga aaaaagtaaa cgaaaatcct gaacttgaga
     3121 aagtatcagc atccgtaaaa ggaaaatcag aagcagccgg ttattatgag cgtattcaac
     3181 ttggaggaat ggctcaaaag atgtttactt ccctgcaaaa agaagtcaag acagatgtgt
     3241 tttctccgct tgaagaagca gcggaagcag caaaagcaaa tgagccaacg ggcgaaacga
     3301 tgcttttaaa cgcgtctttc ttaattaacc gagaagatga agcgaagttt gatgaaaaag
     3361 taaatgaagc gcatgaaaac tggaaagaca aagccgattt tcattacagc ggtccttggc
     3421 ctgcttataa ttttgtgaac attcgcctaa aagtagagga aaaataatca ggagatccta
     3481 gatgggagaa ttactgtatt tatacggttt aattccaaca aaagaagcag cagccataga
     3541 gccgtttcca tcttataagg ggtttgacgg agaacattca ctgtacccaa ttgcgtttga
     3601 tcaggtgacg gctgtagttt ctaagctgga tgctgacacc tattcagaaa aagtgattca
     3661 agaaaaaatg gagcaggata tgagctggct gcaggaaaaa gcatttcatc atcacgaaac
     3721 ggtagccgct ttgtacgaag aatttacgat cattccatta aaattttgca ccatttataa
     3781 aggtgaagaa agtctgcagg cagctattga gattaacaaa gaaaagatag agaattcact
     3841 gacgctgctt caaggaaatg aagagtggaa tgtgaaaatt tactgtgatg atacagagct
     3901 taaaaaagga atcagcgaaa cgaatgaaag cgtgaaagcg aaaaaacaag aaattagtca
     3961 cttatcacca ggaagacagt tttttgaaaa gaaaaaaata gatcagctga ttgaaaaaga
     4021 attagagctt cacaaaaaca aagtgtgtga agagatacat gacaagctaa aagaattatc
     4081 gctttatgac tctgttaaaa agaattggag caaagacgta actggcgcag ctgaacagat
     4141 ggcgtggaac agcgtgtttc ttctcccgtc tctgcagatt actaagttcg taaacgaaat
     4201 agaagagctt cagcaaaggc ttgaaaataa aggctggaag tttgaagtga cgggaccatg
     4261 gccgccctat catttctcga gctttgcgta aaagcggtta agcgtgacag tccaggagga
     4321 attaatggat gcaaccggtc agccaagcaa atggacgaat ccacttggat cctgatcaag
     4381 ctgaacaagg cttagcgcag cttgtgatga cagttattga gctattgagg caaatcgttg
     4441 aacgtcatgc catgaggcgg gtggagggtg gaacgttgac ggacgaacaa attgaaaact
     4501 taggaattgc actaatgaac ttagaagaaa aaatggacga gttgaaagag gtgttcggtc
     4561 tggatgcaga agatttaaat attgatcttg gaccgctagg cagcctgctt taagcggtca
     4621 gtaggaggaa cagtatggca gtcgaacata atatgcagtc aagtacgatt gtagatgtgc
     4681 tcgaaaagat tttggataaa ggagtcgtta tagcggggga catcaccgta ggaattgcag
     4741 atgtcgagct attaacgata aagatccgct tgattgtggc ttcggttgat aaggcaaaag
     4801 aaatcggcat ggactggtgg gaaaatgatc cgtatctcag ttcaaaagga gccaataaca
     4861 aagcgctcga agaagaaaat aaaatgctgc atgagcggtt aaaaacgctt gaagaaaaaa
     4921 tagaaacgaa acgttaaaaa ctgtacgcta cttaaaaaat ggagggattt acaatggcaa
     4981 ctgaaacaaa attagataac acacaggcag aaaacaagga aaataaaaat gcggaaaacg
     5041 gttcaaaaga aaagaacggt tcaaaagcaa gcaaaacaac aagcagcggg ccaatcaaac
     5101 gagcggtagc aggaggcatc atcggtgcaa cgattggata tgtatcgact cctgaaaatc
     5161 gaaaaagtct ccttgaccgc attgatacag acgaattaaa aagcaaagca tctgatttag
     5221 gaacaaaggt aaaagaaaaa tcaaaaagca gcgtggccag cctgaaaaca tctgcgggaa
     5281 gcttgtttaa aaaagataaa gataaatcaa aagatgatga agaaaacgta aattcttcta
     5341 gtagcgaaac agaagacgat aacgttcaag agtacgacga gttaaaagaa gaaaatcaaa
     5401 ctcttcaaga tcgcttatca cagcttgaag aaaaaatgaa catgcttgtt gagcttagcc
     5461 tcaataaaaa tcaagacgaa gaagcggaag atacagattc cgacgaagaa gagaacgatg
     5521 agaacgatga aaacgatgaa aacgagcagg acgatgaaaa cgaagaagaa acatctaagc
     5581 cacgtaaaaa ggataaaaaa gaagctgagg aagaagaaag tgaaagtgac gaagacagcg
     5641 aggaagaaga ggaagattct cgctcaaaca aaaaaaataa aaaagtaaaa acagaagaag
     5701 aagacgaaga tgaaagcgaa gaagaaaaaa aggaagcgaa accaaaaaag tcaacagcta
     5761 aaaaatcaaa aaatacaaaa gcaaagaaaa acacggacga agaagatgat gaagcaacat
     5821 ctctttctag tgaagacgat acaacagcct aagacgtaaa ggaggaaaga aagacatgag
     5881 tacaggccct tctttttcaa ctaaagacaa tacgcttgaa tactttgtga aagcttctaa
     5941 taaacacggc ttttcacttg atatttcatt aaatgtaaac ggcgctgtga tttccggtac
     6001 catgatttca gcaaaagaat actttgatta cttaagcgaa acgtttgaag aaggcagtga
     6061 agtggctcag gcgctaagcg aacaattctc tttagcaagc gaagcgagcg aatcaaacgg
     6121 agaagcagaa gcccatttta ttcatttgaa aaatacaaag atttactgtg gagacagtaa
     6181 atctactcct tctaaaggca aaatcttttg gagagggaaa atagcagaag tagacgggtt
     6241 tttcttagga aagatttctg atgcaaaatc aacgagtaaa aagagttcat aaaaaacggc
     6301 ggggtgattg ccccgccgtt ttttagtgat gtgatgagat gtgcagcttc ttttttcgat
     6361 acatgagcgg agtcatcttc atttcttttc gaaactgatt aataaaatag cttgtactat
     6421 taaaccctac ttgataggcg acttccgtca catttgcttc tgcttgctgc agtccggatt
     6481 cgcgaacgcg tctcgagaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     6541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     6601 ggttttttgc tgaaaggagg aactatatcc gactagtagc ccgcctaatg agcgggcttt
     6661 tttttaattc ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6841 cagaaacgct cgtgaaagta aaagacgcag aggaccaatt gggggcacga gtgggataca
     6901 tagaactgga cttgaatagc ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc
     6961 caatgatgag cactttcaaa gttctgctat gtggagcagt attatcccgt gtagatgcgg
     7021 ggcaagagca actcggacga cgaatacact attcgcagaa tgacttggtt gaatactccc
     7081 cagtgacaga aaagcacctt acggacggaa tgacggtaag agaattatgt agtgccgcca
     7141 taacgatgag tgataacact gcggcgaact tacttctgac aaccatcggt ggaccgaagg
     7201 aattaaccgc ttttttgcac aatatgggag accatgtaac tcgccttgac cgttgggaac
     7261 cagaactgaa tgaagccata ccaaacgacg agcgagacac cacaatgcct gcggcaatgg
     7321 caacaacatt acgcaaacta ttaactggcg aactacttac tctggcttca cggcaacaat
     7381 taatagactg gcttgaagcg gataaagttg caggaccact actgcgttcg gcacttcctg
     7441 ctggctggtt tattgctgat aaatctgggg caggagagcg tggttcacgg ggtatcattg
     7501 ccgcacttgg accagatggt aagccttccc gtatcgtagt tatctacacg acgggtagtc
     7561 aggcaactat ggacgaacga aatagacaga ttgctgaaat aggggcttca ctgattaagc
     7621 attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg acggaccggt
     7681 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7741 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7801 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7861 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7921 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7981 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8041 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8101 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8161 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8221 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8281 cctatggaaa
//
