LOCUS       Exported                7956 bp ds-DNA     circular SYN 27-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100^1101
                     /label=VB
     misc_feature    1108..1116
                     /label=RBS
     CDS             1122..1388
                     /codon_start=1
                     /label=GvpB
                     /translation="MSIQKSTNSSSLAEVIDRILDKGIVIDAFARVSVVGIEILTIEAR
                     VVIASVDTWLRYAEAVGLLRDDVEENGLPERSNSSEGQPRFSI"
     primer_bind     1411..1436
                     /label=Bm1seq
     primer_bind     complement(1420..1435)
                     /label=gvpB_rev
     misc_feature    1435^1436
                     /label=BR
     primer_bind     1436..1458
                     /label=gvpR_fwd
     misc_feature    1449..1457
                     /label=RBS
     CDS             1464..1730
                     /codon_start=1
                     /label=GvpR
                     /translation="MEIKKIMQAVNDFFGEHVAPPHKITSVEATEDEGWRVIVEVIEER
                     EYMKKYAKDEMLGTYECFVNKEKEVISFKRLDVRYRSAIGIEA"
     primer_bind     complement(1721..1742)
                     /label=gvpR_rev
     misc_feature    1742^1743
                     /label=RN
     primer_bind     1743..1763
                     /label=gvpN_fwd
     misc_feature    1744..1752
                     /label=RBS
     CDS             1759..2685
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1789..1814
                     /label=Bm1.1seqF
     misc_feature    1821
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     2191..2210
                     /label=Bm2seqF
     primer_bind     complement(2656..2685)
                     /label=gvpN_rev
     misc_feature    2685^2686
                     /label=NF
     primer_bind     2686..2705
                     /label=gvpF_fwd
     misc_feature    2686..2694
                     /label=RBS
     CDS             2700..3467
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2933..2960
                     /label=Bm3seqF
     primer_bind     complement(3432..3452)
                     /label=gvpF_rev
     misc_feature    3452^3453
                     /label=FG
     primer_bind     3453..3475
                     /label=gvpG_fwd
     misc_feature    3457..3465
                     /label=RBS
     CDS             3469..3735
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3681..3708
                     /label=Bm4seqF
     primer_bind     complement(3699..3720)
                     /label=gvpG_rev
     misc_feature    3720^3721
                     /label=GL
     primer_bind     3721..3740
                     /label=gvpL_fwd
     misc_feature    3722..3730
                     /label=RBS
     CDS             3736..4545
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3806
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3875
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4325
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4456..4482
                     /label=Bm5seqF
     primer_bind     complement(4529..4546)
                     /label=gvpL_rev
     misc_feature    4546
                     /label=LS
     primer_bind     4547..4562
                     /label=gvpK_fwd
     misc_feature    4547
                     /label=SK
     misc_feature    4568..4576
                     /label=RBS
     CDS             4583..4867
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4858..4873)
                     /label=gvpK_rev
     misc_feature    4873^4874
                     /label=KJ
     primer_bind     4874..4892
                     /label=gvpJ_fwd
     misc_feature    4877..4885
                     /label=RBS
     CDS             4889..5191
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    5014
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5020..5041
                     /label=Bm6seqF
     primer_bind     complement(5186..5207)
                     /label=gvpJ_rev
     misc_feature    5207^5208
                     /label=JT
     primer_bind     5208..5231
                     /label=gvpT_fwd
     misc_feature    5214..5222
                     /label=RBS
     CDS             5228..6106
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     5249..5275
                     /label=Bm6.1seq
     misc_feature    5552
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    5615
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5689..5715
                     /label=Bm7seqF
     primer_bind     complement(6094..6111)
                     /label=gvpT_rev
     misc_feature    6111
                     /label=TU
     primer_bind     6112..6129
                     /label=vector_fwd
     misc_feature    6112
                     /label=UV
     terminator      6228..6275
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6342..6433
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6423..6428
                     /label=Emopec_63.3%
     CDS             6434..7294
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7368..7956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat aagcgaaagg agaaatgaga tatgtctatt caaaaaagta
     1141 ctaatagttc aagtttagca gaagtcattg accgtatttt agataaagga attgttattg
     1201 atgcttttgc aagagtttct gttgtaggaa ttgaaatttt aacgattgaa gcgcgagtgg
     1261 ttattgccag tgttgataca tggttacgct atgcagaagc agtagggctt cttcgtgacg
     1321 acgtagaaga aaacggtctt cctgaacgtt caaattcaag tgaagggcag ccgcgtttta
     1381 gtatttaata tgaagtggta tggaaccttc cttttttgag ggtggttcca gcttttagat
     1441 ctaactattg gaggctacta aaaatggaaa ttaaaaaaat tatgcaagcc gtgaacgact
     1501 ttttcggtga acacgtagct cctcctcata aaattacctc ggtggaagct actgaagatg
     1561 aaggttggag agttattgtt gaagtcattg aagaacgaga atatatgaaa aaatacgcca
     1621 aagatgaaat gctcggaacg tacgagtgct ttgtaaataa agaaaaagaa gtcatttcat
     1681 tcaaacgact cgacgtcaga tatagaagcg ccattggcat tgaagcataa acgataagat
     1741 ggcaggagga acgtaaaaat gaccgtctta acagacaaaa ggaaaaaagg cagtggagct
     1801 tttatacaag atgacgagac aaaagaggtt ctttcaagag cgctgagcta tttaaaatcc
     1861 ggctattcca ttcattttac aggtcctgcc ggcggaggca aaacctcttt agcgcgagcg
     1921 cttgctaaaa agagaaagcg tcctgtaatg ctgatgcacg ggaatcacga gctcaacaac
     1981 aaagatttaa ttggcgattt tacgggatac acgagcaaaa aagtaatcga ccagtacgtt
     2041 cgttctgtct ataaaaaaga tgaacaggtg agtgaaaact ggcaggatgg ccgattgctt
     2101 gaagctgtaa aaaatggcta tacgctgatt tacgacgaat ttactcgttc taagcctgcg
     2161 acgaataata tctttctatc gatattagaa gaaggcgtgc tgccgctgta tggagtaaaa
     2221 atgaccgatc cttttgtgcg cgtgcatccc gatttccgcg tcatcttcac aagcaatcca
     2281 gctgagtatg ccggcgtata tgatacgcaa gatgcgcttc tcgacaggtt aattaccatg
     2341 tttattgatt ataaagacat cgacagagag acagcgattt taacggagaa aacggacgta
     2401 gaagaagatg aagcgcgcac aattgtaacg ctcgtagcaa acgtgcgaaa ccgctctgga
     2461 gacgaaaaca gcagcggact tagcctgcgg gcttcgctta tgatcgctac ccttgccacg
     2521 cagcaagaca ttcctatcga tggaagtgac gaagattttc aaacgttatg tatcgatatt
     2581 ttgcatcatc cgcttaccaa atgtttggat gaagaaaatg caaaaagcaa agccgaaaaa
     2641 atcattttag aagaatgtaa gaatatagac actgaagaaa agtaaaggag cttgaaaaca
     2701 tgagtgaaac aaacgaaaca ggtatttata tttttagcgc cattcaaacg gataaagacg
     2761 aagaatttgg cgccgtggaa gtagaaggaa caaaagctga aacatttttg attcgctaca
     2821 aagacgcggc tatggtagca gctgaagtac cgatgaaaat ttatcatcct aatcgccaaa
     2881 atttattaat gcatcaaaac gcagtagcag cgattatgga caagaacgat acggttattc
     2941 caatcagctt tgggaatgta ttcaaatcaa aagaagacgt aaaagttctt ttggaaaacc
     3001 tttatccgca gtttgaaaag ctgtttccag cgatcaaagg aaaaattgaa gtcggtttaa
     3061 aagtaattgg gaaaaaagaa tggcttgaga aaaaagtaaa cgaaaatcct gaacttgaga
     3121 aagtatcagc atccgtaaaa ggaaaatcag aagcagccgg ttattatgag cgtattcaac
     3181 ttggaggaat ggctcaaaag atgtttactt ccctgcaaaa agaagtcaag acagatgtgt
     3241 tttctccgct tgaagaagca gcggaagcag caaaagcaaa tgagccaacg ggcgaaacga
     3301 tgcttttaaa cgcgtctttc ttaattaacc gagaagatga agcgaagttt gatgaaaaag
     3361 taaatgaagc gcatgaaaac tggaaagaca aagccgattt tcattacagc ggtccttggc
     3421 ctgcttataa ttttgtgaac attcgcctaa aagtagaaga gaaataacgt gcttcacaaa
     3481 ttagtaaccg cacccattaa ccttgtagtg aaaatcggcg aaaaagtaca ggaagaagct
     3541 gataaacagc tatatgacct tccgacgatt cagcaaaagc tcattcagct tcaaatgatg
     3601 tttgagcttg gtgaaattcc agaagaagcg tttcaagaaa aagaagatga attgttaatg
     3661 aggtacgaaa ttgcgaaacg cagagaaatt gaacaatggg aagagctaac acaaaaaaga
     3721 aatgaggaat cctagatggg agaattactg tatttatacg gtttaattcc aacaaaagaa
     3781 gcagcagcca tagagccgtt tccatcttat aaggggtttg acggagaaca ttcactgtac
     3841 ccaattgcgt ttgatcaggt gacggctgta gtttctaagc tggatgctga cacctattca
     3901 gaaaaagtga ttcaagaaaa aatggagcag gatatgagct ggctgcagga aaaagcattt
     3961 catcatcacg aaacggtagc cgctttgtac gaagaattta cgatcattcc attaaaattt
     4021 tgcaccattt ataaaggtga agaaagtctg caggcagcta ttgagattaa caaagaaaag
     4081 atagagaatt cactgacgct gcttcaagga aatgaagagt ggaatgtgaa aatttactgt
     4141 gatgatacag agcttaaaaa aggaatcagc gaaacgaatg aaagcgtgaa agcgaaaaaa
     4201 caagaaatta gtcacttatc accaggaaga cagttttttg aaaagaaaaa aatagatcag
     4261 ctgattgaaa aagaattaga gcttcacaaa aacaaagtgt gtgaagagat acatgacaag
     4321 ctaaaagaat tatcgcttta tgactctgtt aaaaagaatt ggagcaaaga cgtaactggc
     4381 gcagctgaac agatggcgtg gaacagcgtg tttcttctcc cgtctctgca gattactaag
     4441 ttcgtaaacg aaatagaaga gcttcagcaa aggcttgaaa ataaaggctg gaagtttgaa
     4501 gtgacgggac catggccgcc ctatcatttc tcgagctttg cgtaaaagcg gttaagcgtg
     4561 acagtccagg aggaattaat ggatgcaacc ggtcagccaa gcaaatggac gaatccactt
     4621 ggatcctgat caagctgaac aaggcttagc gcagcttgtg atgacagtta ttgagctatt
     4681 gaggcaaatc gttgaacgtc atgccatgag gcgggtggag ggtggaacgt tgacggacga
     4741 acaaattgaa aacttaggaa ttgcactaat gaacttagaa gaaaaaatgg acgagttgaa
     4801 agaggtgttc ggtctggatg cagaagattt aaatattgat cttggaccgc taggcagcct
     4861 gctttaagcg gtcagtagga ggaacagtat ggcagtcgaa cataatatgc agtcaagtac
     4921 gattgtagat gtgctcgaaa agattttgga taaaggagtc gttatagcgg gggacatcac
     4981 cgtaggaatt gcagatgtcg agctattaac gataaagatc cgcttgattg tggcttcggt
     5041 tgataaggca aaagaaatcg gcatggactg gtgggaaaat gatccgtatc tcagttcaaa
     5101 aggagccaat aacaaagcgc tcgaagaaga aaataaaatg ctgcatgagc ggttaaaaac
     5161 gcttgaagaa aaaatagaaa cgaaacgtta aaaactgtac gctacttaaa aaatggaggg
     5221 atttacaatg gcaactgaaa caaaattaga taacacacag gcagaaaaca aggaaaataa
     5281 aaatgcggaa aacggttcaa aagaaaagaa cggttcaaaa gcaagcaaaa caacaagcag
     5341 cgggccaatc aaacgagcgg tagcaggagg catcatcggt gcaacgattg gatatgtatc
     5401 gactcctgaa aatcgaaaaa gtctccttga ccgcattgat acagacgaat taaaaagcaa
     5461 agcatctgat ttaggaacaa aggtaaaaga aaaatcaaaa agcagcgtgg ccagcctgaa
     5521 aacatctgcg ggaagcttgt ttaaaaaaga taaagataaa tcaaaagatg atgaagaaaa
     5581 cgtaaattct tctagtagcg aaacagaaga cgataacgtt caagagtacg acgagttaaa
     5641 agaagaaaat caaactcttc aagatcgctt atcacagctt gaagaaaaaa tgaacatgct
     5701 tgttgagctt agcctcaata aaaatcaaga cgaagaagcg gaagatacag attccgacga
     5761 agaagagaac gatgagaacg atgaaaacga tgaaaacgag caggacgatg aaaacgaaga
     5821 agaaacatct aagccacgta aaaaggataa aaaagaagct gaggaagaag aaagtgaaag
     5881 tgacgaagac agcgaggaag aagaggaaga ttctcgctca aacaaaaaaa ataaaaaagt
     5941 aaaaacagaa gaagaagacg aagatgaaag cgaagaagaa aaaaaggaag cgaaaccaaa
     6001 aaagtcaaca gctaaaaaat caaaaaatac aaaagcaaag aaaaacacgg acgaagaaga
     6061 tgatgaagca acatctcttt ctagtgaaga cgatacaaca gcctaagacg tcgtcacatt
     6121 tgcttctgct tgctgcagtc cggattcgcg aacgcgtctc gagagatccg gctgctaaca
     6181 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     6241 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccgact
     6301 agtagcccgc ctaatgagcg ggcttttttt taattcccct atttgtttat ttttctaaat
     6361 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6421 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6481 attttgcctt cctgtttttg ctcacccaga aacgctcgtg aaagtaaaag acgcagagga
     6541 ccaattgggg gcacgagtgg gatacataga actggacttg aatagcggta aaatccttga
     6601 gagttttcgc cctgaagagc gttttccaat gatgagcact ttcaaagttc tgctatgtgg
     6661 agcagtatta tcccgtgtag atgcggggca agagcaactc ggacgacgaa tacactattc
     6721 gcagaatgac ttggttgaat actccccagt gacagaaaag caccttacgg acggaatgac
     6781 ggtaagagaa ttatgtagtg ccgccataac gatgagtgat aacactgcgg cgaacttact
     6841 tctgacaacc atcggtggac cgaaggaatt aaccgctttt ttgcacaata tgggagacca
     6901 tgtaactcgc cttgaccgtt gggaaccaga actgaatgaa gccataccaa acgacgagcg
     6961 agacaccaca atgcctgcgg caatggcaac aacattacgc aaactattaa ctggcgaact
     7021 acttactctg gcttcacggc aacaattaat agactggctt gaagcggata aagttgcagg
     7081 accactactg cgttcggcac ttcctgctgg ctggtttatt gctgataaat ctggggcagg
     7141 agagcgtggt tcacggggta tcattgccgc acttggacca gatggtaagc cttcccgtat
     7201 cgtagttatc tacacgacgg gtagtcaggc aactatggac gaacgaaata gacagattgc
     7261 tgaaataggg gcttcactga ttaagcattg gtaaaccgat acaattaaag gctccttttg
     7321 gagccttttt ttttggacgg accggtagaa aagatcaaag gatcttcttg agatcctttt
     7381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7501 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7921 ttgtgatgct cgtcaggggg gcggagccta tggaaa
//
