LOCUS       Exported                7902 bp ds-DNA     circular SYN 15-DEC-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Lu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 18, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    187..326
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(428..619)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        856..874
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1075..1100)
                     /label=vector_rev
     misc_feature    1100
                     /label=VB
     primer_bind     1101..1121
                     /label=gvpN_fwd
     misc_feature    1101
                     /label=RN
     misc_feature    1102..1110
                     /label=RBS
     CDS             1117..2043
                     /codon_start=1
                     /label=GvpN
                     /translation="MTVLTDKRKKGSGAFIQDDETKEVLSRALSYLKSGYSIHFTGPAG
                     GGKTSLARALAKKRKRPVMLMHGNHELNNKDLIGDFTGYTSKKVIDQYVRSVYKKDEQV
                     SENWQDGRLLEAVKNGYTLIYDEFTRSKPATNNIFLSILEEGVLPLYGVKMTDPFVRVH
                     PDFRVIFTSNPAEYAGVYDTQDALLDRLITMFIDYKDIDRETAILTEKTDVEEDEARTI
                     VTLVANVRNRSGDENSSGLSLRASLMIATLATQQDIPIDGSDEDFQTLCIDILHHPLTK
                     CLDEENAKSKAEKIILEECKNIDTEEK"
     primer_bind     1147..1172
                     /label=Bm1.1seqF
     misc_feature    1179
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     1549..1568
                     /label=Bm2seqF
     primer_bind     complement(2014..2043)
                     /label=gvpN_rev
     misc_feature    2043^2044
                     /label=NF
     primer_bind     2044..2063
                     /label=gvpF_fwd
     misc_feature    2044..2052
                     /label=RBS
     CDS             2058..2825
                     /codon_start=1
                     /label=GvpF
                     /translation="MSETNETGIYIFSAIQTDKDEEFGAVEVEGTKAETFLIRYKDAAM
                     VAAEVPMKIYHPNRQNLLMHQNAVAAIMDKNDTVIPISFGNVFKSKEDVKVLLENLYPQ
                     FEKLFPAIKGKIEVGLKVIGKKEWLEKKVNENPELEKVSASVKGKSEAAGYYERIQLGG
                     MAQKMFTSLQKEVKTDVFSPLEEAAEAAKANEPTGETMLLNASFLINREDEAKFDEKVN
                     EAHENWKDKADFHYSGPWPAYNFVNIRLKVEEK"
     primer_bind     2291..2318
                     /label=Bm3seqF
     primer_bind     complement(2790..2810)
                     /label=gvpF_rev
     misc_feature    2810^2811
                     /label=FG
     primer_bind     2811..2833
                     /label=gvpG_fwd
     misc_feature    2815..2823
                     /label=RBS
     CDS             2827..3093
                     /codon_start=1
                     /label=GvpG
                     /translation="VLHKLVTAPINLVVKIGEKVQEEADKQLYDLPTIQQKLIQLQMMF
                     ELGEIPEEAFQEKEDELLMRYEIAKRREIEQWEELTQKRNEES"
     primer_bind     3039..3066
                     /label=Bm4seqF
     primer_bind     complement(3057..3078)
                     /label=gvpG_rev
     misc_feature    3078^3079
                     /label=GL
     primer_bind     3079..3098
                     /label=gvpL_fwd
     misc_feature    3080..3088
                     /label=RBS
     CDS             3094..3903
                     /codon_start=1
                     /label=GvpL
                     /translation="MGELLYLYGLIPTKEAAAIEPFPSYKGFDGEHSLYPIAFDQVTAV
                     VSKLDADTYSEKVIQEKMEQDMSWLQEKAFHHHETVAALYEEFTIIPLKFCTIYKGEES
                     LQAAIEINKEKIENSLTLLQGNEEWNVKIYCDDTELKKGISETNESVKAKKQEISHLSP
                     GRQFFEKKKIDQLIEKELELHKNKVCEEIHDKLKELSLYDSVKKNWSKDVTGAAEQMAW
                     NSVFLLPSLQITKFVNEIEELQQRLENKGWKFEVTGPWPPYHFSSFA"
     misc_feature    3164
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3233
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    3683
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     3814..3840
                     /label=Bm5seqF
     primer_bind     complement(3887..3904)
                     /label=gvpL_rev
     misc_feature    3904
                     /label=LS
     primer_bind     3905..3920
                     /label=gvpK_fwd
     misc_feature    3905
                     /label=SK
     misc_feature    3926..3934
                     /label=RBS
     CDS             3941..4225
                     /codon_start=1
                     /label=gvpK
                     /translation="MQPVSQANGRIHLDPDQAEQGLAQLVMTVIELLRQIVERHAMRRV
                     EGGTLTDEQIENLGIALMNLEEKMDELKEVFGLDAEDLNIDLGPLGSLL"
     primer_bind     complement(4216..4231)
                     /label=gvpK_rev
     misc_feature    4231^4232
                     /label=KJ
     primer_bind     4232..4250
                     /label=gvpJ_fwd
     misc_feature    4235..4243
                     /label=RBS
     CDS             4247..4549
                     /codon_start=1
                     /label=GvpJ
                     /translation="MAVEHNMQSSTIVDVLEKILDKGVVIAGDITVGIADVELLTIKIR
                     LIVASVDKAKEIGMDWWENDPYLSSKGANNKALEEENKMLHERLKTLEEKIETKR"
     misc_feature    4372
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     4378..4399
                     /label=Bm6seqF
     primer_bind     complement(4544..4565)
                     /label=gvpJ_rev
     misc_feature    4565^4566
                     /label=JT
     primer_bind     4566..4589
                     /label=gvpT_fwd
     misc_feature    4572..4580
                     /label=RBS
     CDS             4586..5464
                     /codon_start=1
                     /label=GvpT
                     /translation="MATETKLDNTQAENKENKNAENGSKEKNGSKASKTTSSGPIKRAV
                     AGGIIGATIGYVSTPENRKSLLDRIDTDELKSKASDLGTKVKEKSKSSVASLKTSAGSL
                     FKKDKDKSKDDEENVNSSSSETEDDNVQEYDELKEENQTLQDRLSQLEEKMNMLVELSL
                     NKNQDEEAEDTDSDEEENDENDENDENEQDDENEEETSKPRKKDKKEAEEEESESDEDS
                     EEEEEDSRSNKKNKKVKTEEEDEDESEEEKKEAKPKKSTAKKSKNTKAKKNTDEEDDEA
                     TSLSSEDDTTA"
     primer_bind     4607..4633
                     /label=Bm6.1seq
     misc_feature    4910
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     misc_feature    4973
                     /label=SNP
                     /note="Bacillus megaterium QM B1551 SNP"
     primer_bind     5047..5073
                     /label=Bm7seqF
     primer_bind     complement(5452..5469)
                     /label=gvpT_rev
     misc_feature    5469^5470
                     /label=TU
     primer_bind     5470..5490
                     /label=gvpU_fwd
     misc_feature    5472..5480
                     /label=RBS
     CDS             5488..5904
                     /codon_start=1
                     /label=GvpU
                     /translation="MSTGPSFSTKDNTLEYFVKASNKHGFSLDISLNVNGAVISGTMIS
                     AKEYFDYLSETFEEGSEVAQALSEQFSLASEASESNGEAEAHFIHLKNTKIYCGDSKST
                     PSKGKIFWRGKIAEVDGFFLGKISDAKSTSKKSS"
     primer_bind     5827..5851
                     /label=GvpUseqF
     misc_feature    6057^6058
                     /label=UV
     primer_bind     6058..6075
                     /label=vector_fwd
     terminator      6174..6221
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6288..6379
                     /gene="bla"
                     /label=AmpR promoter
     RBS             6369..6374
                     /label=Emopec_63.3%
     CDS             6380..7240
                     /codon_start=1
                     /label=Beta-lactamase
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWLEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7314..7902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aacgccagca acgcggccct ttttacggtt cctggccttt tgctggcctt ttgctcacat
       61 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
      121 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      181 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg
      241 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
      301 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
      361 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      421 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
      481 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
      541 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
      601 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atggggtaat
      661 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
      721 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
      781 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttg gcaccatgca
      841 tcgatcccgc gaaattaata cgactcacta tagggagacc acaacggttt ccctctagag
      901 atagcttagc gatgtgaaag aagctggaga aaactttcaa gaaaaagttt cttcttcaga
      961 cgataaagag aagtcaaaaa ataagcggaa aataaaaggc gtaaatcaga ttaaaaaagc
     1021 aacggatatt aaaagctcta ctaagattaa aagttctaat gatattaaat catctacaga
     1081 tttaaaaaca atgggatcat caggaggaac gtaaaaatga ccgtcttaac agacaaaagg
     1141 aaaaaaggca gtggagcttt tatacaagat gacgagacaa aagaggttct ttcaagagcg
     1201 ctgagctatt taaaatccgg ctattccatt cattttacag gtcctgccgg cggaggcaaa
     1261 acctctttag cgcgagcgct tgctaaaaag agaaagcgtc ctgtaatgct gatgcacggg
     1321 aatcacgagc tcaacaacaa agatttaatt ggcgatttta cgggatacac gagcaaaaaa
     1381 gtaatcgacc agtacgttcg ttctgtctat aaaaaagatg aacaggtgag tgaaaactgg
     1441 caggatggcc gattgcttga agctgtaaaa aatggctata cgctgattta cgacgaattt
     1501 actcgttcta agcctgcgac gaataatatc tttctatcga tattagaaga aggcgtgctg
     1561 ccgctgtatg gagtaaaaat gaccgatcct tttgtgcgcg tgcatcccga tttccgcgtc
     1621 atcttcacaa gcaatccagc tgagtatgcc ggcgtatatg atacgcaaga tgcgcttctc
     1681 gacaggttaa ttaccatgtt tattgattat aaagacatcg acagagagac agcgatttta
     1741 acggagaaaa cggacgtaga agaagatgaa gcgcgcacaa ttgtaacgct cgtagcaaac
     1801 gtgcgaaacc gctctggaga cgaaaacagc agcggactta gcctgcgggc ttcgcttatg
     1861 atcgctaccc ttgccacgca gcaagacatt cctatcgatg gaagtgacga agattttcaa
     1921 acgttatgta tcgatatttt gcatcatccg cttaccaaat gtttggatga agaaaatgca
     1981 aaaagcaaag ccgaaaaaat cattttagaa gaatgtaaga atatagacac tgaagaaaag
     2041 taaaggagct tgaaaacatg agtgaaacaa acgaaacagg tatttatatt tttagcgcca
     2101 ttcaaacgga taaagacgaa gaatttggcg ccgtggaagt agaaggaaca aaagctgaaa
     2161 catttttgat tcgctacaaa gacgcggcta tggtagcagc tgaagtaccg atgaaaattt
     2221 atcatcctaa tcgccaaaat ttattaatgc atcaaaacgc agtagcagcg attatggaca
     2281 agaacgatac ggttattcca atcagctttg ggaatgtatt caaatcaaaa gaagacgtaa
     2341 aagttctttt ggaaaacctt tatccgcagt ttgaaaagct gtttccagcg atcaaaggaa
     2401 aaattgaagt cggtttaaaa gtaattggga aaaaagaatg gcttgagaaa aaagtaaacg
     2461 aaaatcctga acttgagaaa gtatcagcat ccgtaaaagg aaaatcagaa gcagccggtt
     2521 attatgagcg tattcaactt ggaggaatgg ctcaaaagat gtttacttcc ctgcaaaaag
     2581 aagtcaagac agatgtgttt tctccgcttg aagaagcagc ggaagcagca aaagcaaatg
     2641 agccaacggg cgaaacgatg cttttaaacg cgtctttctt aattaaccga gaagatgaag
     2701 cgaagtttga tgaaaaagta aatgaagcgc atgaaaactg gaaagacaaa gccgattttc
     2761 attacagcgg tccttggcct gcttataatt ttgtgaacat tcgcctaaaa gtagaagaga
     2821 aataacgtgc ttcacaaatt agtaaccgca cccattaacc ttgtagtgaa aatcggcgaa
     2881 aaagtacagg aagaagctga taaacagcta tatgaccttc cgacgattca gcaaaagctc
     2941 attcagcttc aaatgatgtt tgagcttggt gaaattccag aagaagcgtt tcaagaaaaa
     3001 gaagatgaat tgttaatgag gtacgaaatt gcgaaacgca gagaaattga acaatgggaa
     3061 gagctaacac aaaaaagaaa tgaggaatcc tagatgggag aattactgta tttatacggt
     3121 ttaattccaa caaaagaagc agcagccata gagccgtttc catcttataa ggggtttgac
     3181 ggagaacatt cactgtaccc aattgcgttt gatcaggtga cggctgtagt ttctaagctg
     3241 gatgctgaca cctattcaga aaaagtgatt caagaaaaaa tggagcagga tatgagctgg
     3301 ctgcaggaaa aagcatttca tcatcacgaa acggtagccg ctttgtacga agaatttacg
     3361 atcattccat taaaattttg caccatttat aaaggtgaag aaagtctgca ggcagctatt
     3421 gagattaaca aagaaaagat agagaattca ctgacgctgc ttcaaggaaa tgaagagtgg
     3481 aatgtgaaaa tttactgtga tgatacagag cttaaaaaag gaatcagcga aacgaatgaa
     3541 agcgtgaaag cgaaaaaaca agaaattagt cacttatcac caggaagaca gttttttgaa
     3601 aagaaaaaaa tagatcagct gattgaaaaa gaattagagc ttcacaaaaa caaagtgtgt
     3661 gaagagatac atgacaagct aaaagaatta tcgctttatg actctgttaa aaagaattgg
     3721 agcaaagacg taactggcgc agctgaacag atggcgtgga acagcgtgtt tcttctcccg
     3781 tctctgcaga ttactaagtt cgtaaacgaa atagaagagc ttcagcaaag gcttgaaaat
     3841 aaaggctgga agtttgaagt gacgggacca tggccgccct atcatttctc gagctttgcg
     3901 taaaagcggt taagcgtgac agtccaggag gaattaatgg atgcaaccgg tcagccaagc
     3961 aaatggacga atccacttgg atcctgatca agctgaacaa ggcttagcgc agcttgtgat
     4021 gacagttatt gagctattga ggcaaatcgt tgaacgtcat gccatgaggc gggtggaggg
     4081 tggaacgttg acggacgaac aaattgaaaa cttaggaatt gcactaatga acttagaaga
     4141 aaaaatggac gagttgaaag aggtgttcgg tctggatgca gaagatttaa atattgatct
     4201 tggaccgcta ggcagcctgc tttaagcggt cagtaggagg aacagtatgg cagtcgaaca
     4261 taatatgcag tcaagtacga ttgtagatgt gctcgaaaag attttggata aaggagtcgt
     4321 tatagcgggg gacatcaccg taggaattgc agatgtcgag ctattaacga taaagatccg
     4381 cttgattgtg gcttcggttg ataaggcaaa agaaatcggc atggactggt gggaaaatga
     4441 tccgtatctc agttcaaaag gagccaataa caaagcgctc gaagaagaaa ataaaatgct
     4501 gcatgagcgg ttaaaaacgc ttgaagaaaa aatagaaacg aaacgttaaa aactgtacgc
     4561 tacttaaaaa atggagggat ttacaatggc aactgaaaca aaattagata acacacaggc
     4621 agaaaacaag gaaaataaaa atgcggaaaa cggttcaaaa gaaaagaacg gttcaaaagc
     4681 aagcaaaaca acaagcagcg ggccaatcaa acgagcggta gcaggaggca tcatcggtgc
     4741 aacgattgga tatgtatcga ctcctgaaaa tcgaaaaagt ctccttgacc gcattgatac
     4801 agacgaatta aaaagcaaag catctgattt aggaacaaag gtaaaagaaa aatcaaaaag
     4861 cagcgtggcc agcctgaaaa catctgcggg aagcttgttt aaaaaagata aagataaatc
     4921 aaaagatgat gaagaaaacg taaattcttc tagtagcgaa acagaagacg ataacgttca
     4981 agagtacgac gagttaaaag aagaaaatca aactcttcaa gatcgcttat cacagcttga
     5041 agaaaaaatg aacatgcttg ttgagcttag cctcaataaa aatcaagacg aagaagcgga
     5101 agatacagat tccgacgaag aagagaacga tgagaacgat gaaaacgatg aaaacgagca
     5161 ggacgatgaa aacgaagaag aaacatctaa gccacgtaaa aaggataaaa aagaagctga
     5221 ggaagaagaa agtgaaagtg acgaagacag cgaggaagaa gaggaagatt ctcgctcaaa
     5281 caaaaaaaat aaaaaagtaa aaacagaaga agaagacgaa gatgaaagcg aagaagaaaa
     5341 aaaggaagcg aaaccaaaaa agtcaacagc taaaaaatca aaaaatacaa aagcaaagaa
     5401 aaacacggac gaagaagatg atgaagcaac atctctttct agtgaagacg atacaacagc
     5461 ctaagacgta aaggaggaaa gaaagacatg agtacaggcc cttctttttc aactaaagac
     5521 aatacgcttg aatactttgt gaaagcttct aataaacacg gcttttcact tgatatttca
     5581 ttaaatgtaa acggcgctgt gatttccggt accatgattt cagcaaaaga atactttgat
     5641 tacttaagcg aaacgtttga agaaggcagt gaagtggctc aggcgctaag cgaacaattc
     5701 tctttagcaa gcgaagcgag cgaatcaaac ggagaagcag aagcccattt tattcatttg
     5761 aaaaatacaa agatttactg tggagacagt aaatctactc cttctaaagg caaaatcttt
     5821 tggagaggga aaatagcaga agtagacggg tttttcttag gaaagatttc tgatgcaaaa
     5881 tcaacgagta aaaagagttc ataaaaaacg gcggggtgat tgccccgccg ttttttagtg
     5941 atgtgatgag atgtgcagct tcttttttcg atacatgagc ggagtcatct tcatttcttt
     6001 tcgaaactga ttaataaaat agcttgtact attaaaccct acttgatagg cgacttccgt
     6061 cacatttgct tctgcttgct gcagtccgga ttcgcgaacg cgtctcgaga gatccggctg
     6121 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     6181 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     6241 ccgactagta gcccgcctaa tgagcgggct tttttttaat tcccctattt gtttattttt
     6301 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6361 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6421 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctcgtgaaag taaaagacgc
     6481 agaggaccaa ttgggggcac gagtgggata catagaactg gacttgaata gcggtaaaat
     6541 ccttgagagt tttcgccctg aagagcgttt tccaatgatg agcactttca aagttctgct
     6601 atgtggagca gtattatccc gtgtagatgc ggggcaagag caactcggac gacgaataca
     6661 ctattcgcag aatgacttgg ttgaatactc cccagtgaca gaaaagcacc ttacggacgg
     6721 aatgacggta agagaattat gtagtgccgc cataacgatg agtgataaca ctgcggcgaa
     6781 cttacttctg acaaccatcg gtggaccgaa ggaattaacc gcttttttgc acaatatggg
     6841 agaccatgta actcgccttg accgttggga accagaactg aatgaagcca taccaaacga
     6901 cgagcgagac accacaatgc ctgcggcaat ggcaacaaca ttacgcaaac tattaactgg
     6961 cgaactactt actctggctt cacggcaaca attaatagac tggcttgaag cggataaagt
     7021 tgcaggacca ctactgcgtt cggcacttcc tgctggctgg tttattgctg ataaatctgg
     7081 ggcaggagag cgtggttcac ggggtatcat tgccgcactt ggaccagatg gtaagccttc
     7141 ccgtatcgta gttatctaca cgacgggtag tcaggcaact atggacgaac gaaatagaca
     7201 gattgctgaa ataggggctt cactgattaa gcattggtaa accgatacaa ttaaaggctc
     7261 cttttggagc cttttttttt ggacggaccg gtagaaaaga tcaaaggatc ttcttgagat
     7321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7441 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aa
//
