LOCUS       vdh_deletion_in_        4389 bp ds-DNA     circular     17-JUL-2017
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             2936..4357
                     /gene="sacB"
                     /codon_start=1
                     /transl_table=11
                     /product="levan sucrase"
                     /protein_id="ACJ66845.1"
                     /db_xref="GI:215434896"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGIS
                     HITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHG
                     YHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQT
                     QEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIF
                     DGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEES
                     LFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIAS
                     NTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLN
                     KTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLN
                     IKGKKTSVVKDSILEQGQLTVNK"
                     /label=levan sucrase
     promoter        2064..2113
                     /label=NEOKAN Promoter
     rep_origin      1..595
                     /note="pMB1 replication origin"
                     /label=pMB1 replication origin
     gene            2135..2929
                     /gene="nptII"
                     /label=nptII
     CDS             2135..2929
                     /gene="nptII"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase"
                     /protein_id="ACJ66844.1"
                     /db_xref="GI:215434895"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /label=neomycin phosphotransferase
     gene            2936..4357
                     /gene="sacB"
                     /label=sacB
     primer_bind     2444..2462
                     /label=oCJ389
     primer_bind     complement(2037..2054)
                     /note="M13F"
                     /label=M13F
     primer_bind     3089..3107
                     /label=oCJ390
     primer_bind     3727..3745
                     /label=oCJ391
     primer_bind     14..31
                     /label=oCJ392
     -35_signal      2070..2075
                     /label=-35
     -10_signal      2093..2098
                     /label=-10
     primer_bind     601..617
                     /label=M13R
     primer_bind     510..527
                     /label=oCJ546
     primer_bind     complement(2132..2151)
                     /label=oCJ547
     source          complement(1328..2027)
                     /organism="Pseudomonas putida KT2440"
                     /mol_type="genomic DNA"
                     /strain="KT2440"
                     /db_xref="taxon:160488"
                     /label=source:Pseudomonas putida KT2440
     source          complement(632..1321)
                     /organism="Pseudomonas putida KT2440"
                     /mol_type="genomic DNA"
                     /strain="KT2440"
                     /db_xref="taxon:160488"
                     /label=source:Pseudomonas putida KT2440(1)
     gene            1435..2027
                     /gene="fcs"
                     /locus_tag="PP_3356"
                     /label=fcs
     CDS             1435..2027
                     /gene="fcs"
                     /locus_tag="PP_3356"
                     /EC_number="6.2.1.34"
                     /note="Evidence 1b : Function experimentally demonstrated
                     in the studied species; PubMedId : 12534466, 10543794,
                     9579070, 10222033; Product type e : enzyme;
                     Biologicalprocesses : Scavenge (Catabolism)"
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA-synthetase"
                     /protein_id="AAN68960.2"
                     /translation="MNNEARSGSTDPGQRPRYRQVAIGHPQVQVSHVDDVLRMQPVEP
                     LAPLPARLLERLVHWAQVRPDTTFIAARQADGAWRSISYVQMLADVRTIAANLLGLGL
                     SAERPLALLSGNDIEHLQIALGAMYAGIAYCPVSPAYALLSQDFAKLRHVCEVLTPGV
                     VFVSDSQPFQRAFEAVLDDSVGVISVRGQVAGRPHISFDSLLQPGDLAAADAAFAATG
                     PDTIAKFLFTSGSTKLPKAVITTQRMLCANQQMLLQTFPTFAEEPPVLVDWLPWNHTF
                     GGSHNLGIVLYNGGSFYLDAGKPTPQGFAETLRNLREISPTAYLTVPKGWEELVKALE
                     QDPALREVFFARIKLFFFAAAGLSQSVWDRLDRIAEQHCGERIRMMAGLGMTEASPSC
                     TFTTGPLSMAGYVGLPAPGCEVKLVPVGDKLEARFRGPHIMPGYWRSPQQTAEAFDEE
                     GFYCSGDALKLADARQPELGLMFDGRIAEDFKLSSGVFVSVGPLRNRAVLEGSPYVQD
                     IVVTAPDRECLGLLVFPRLPECRRLAGLAEDASDARVLANDTVRSWFADWLERLNRDA
                     QGNASRIEWLSLLAEPPSIDAGEITDKGSINQRAVLQRRAAQVEALYRGEDPDALHAK
                     VRP"
                     /label=feruloyl-CoA-synthetase
     gene            1328..1343
                     /gene="vdh"
                     /locus_tag="PP_3357"
                     /label=vdh
     CDS             1328..1343
                     /gene="vdh"
                     /locus_tag="PP_3357"
                     /EC_number="1.2.1.67"
                     /note="Evidence 1b : Function experimentally demonstrated
                     in the studied species; PubMedId : 12534466, 10543794;
                     Product type e : enzyme; Biologicalprocesses : Scavenge
                     (Catabolism)"
                     /codon_start=1
                     /transl_table=11
                     /product="vanillin dehydrogenase"
                     /protein_id="AAN68961.1"
                     /translation="MLQVPLLIGGQSRPASDGRTFERCNPVTGEVVSQAAAATLADAD
                     AAVAAASAAFPAWAALAPGERRSRLLAGADLLQARAAEFIAAAGETGAMANWYGFNVK
                     LAANMLREAAAMTTQITGEVIPSDVPGSFAMALRAPCGVVLGIAPWNAPVILATRAIA
                     MPLACGNTVVLKASELSPAVHRLIGQVLHDAGIGDGVVNVISNAPQDAPAIVERLIAN
                     PAVRRVNFTGSTHVGRIVGELAARHLKPALLELGGKAPLLVLDDADLDATVEAAAFGA
                     YFNQGQICMSTERLVVDSCIADAFVDKLAVKIAGLRAGDPQASTSVLGSLVSAAAGER
                     IKALIDDAVAKGARLVSGGQLEGSILQPTLLDNVDASMRLYREESFGPVAVVLRAEGD
                     EALLQLANDSEFGLSSAIFSRDTSRALALAQRVESGICHINGPTVHDEAQMPFGGVKS
                     SGYGSFGSRTAIDQFTQLRWVTLQHGPRHYPI"
                     /label=vanillin dehydrogenase
     gene            632..1259
                     /locus_tag="PP_3358"
                     /label=gene(3)
     CDS             632..1259
                     /locus_tag="PP_3358"
                     /EC_number="4.1.2.41"
                     /EC_number="4.2.1.101"
                     /note="Evidence 2b : Function of strongly homologous gene;
                     PubMedId : 12534466, 10543794; Product type e : enzyme;
                     Biologicalprocesses : Scavenge (Catabolism)"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycinnamoyl-CoA hydratase-lyase"
                     /protein_id="AAN68962.1"
                     /translation="MSKYEGRWTTVKVELEAGIAWVTLNRPEKRNAMSPTLNREMVDV
                     LETLEQDADAGVLVLTGAGESWTAGMDLKEYFREVDAGPEILQEKIRREASQWQWKLL
                     RLYAKPTIAMVNGWCFGGGFSPLVACDLAICANEATFGLSEINWGIPPGNLVSKAMAD
                     TVGHRQSLYYIMTGKTFDGRKAAEMGLVNDSVPLAELRETTRELALNLLEKNPVVLRA
                     AKNGFKRCRELTWEQNEDYLYAKLDQSRLLDTTGGREQGMKQFLDDKSIKPGLQAYKR
                     "
                     /label=hydroxycinnamoyl-CoA hydratase-lyase
     misc_feature    632..1321
                     /label=vdh upstream targeting region
     CDS             1549..2027
                     /label=fcs (as annotated in BioCyc)
     misc_feature    complement(1328..2027)
                     /label=vdh downstream targeting region
     primer_bind     553..571
                     /label=oCJ680
     primer_bind     complement(2087..2105)
                     /label=oCJ681
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgcctcaca
      601 caggaaacag ctatgacatg attacGAATT Ccggcatgga cctgaaggag tacttccgcg
      661 aggtggacgc cggcccggaa atcctccagg aaaagattcg tcgcgaagcc tcgcaatggc
      721 aatggaagtt gctgcgtctg tatgccaaac cgaccatcgc catggtcaac ggctggtgct
      781 tcggcggcgg cttcagccca ctggtggcat gcgacctggc gatctgcgcc aacgaagcga
      841 ccttcggcct gtcggaaatc aactggggca tcccgcctgg taacctggtc agcaaggcca
      901 tggccgatac cgttggccat cgtcagtcgc tgtactacat catgaccggc aagaccttcg
      961 atggtcgcaa ggctgccgag atgggcctgg tgaacgacag tgtgccgctg gccgagctgc
     1021 gtgaaaccac ccgcgagttg gcgctgaacc tgctggaaaa gaacccggtg gtgctgcgtg
     1081 ccgcgaagaa tggcttcaag cgttgccgcg agctgacctg ggaacagaac gaggactacc
     1141 tctacgccaa gctcgaccag tcgcgcctgc tggacactac cggcggccgc gagcagggca
     1201 tgaagcagtt cctcgacgac aagagcatca agccaggcct gcaggcctac aagcgctgag
     1261 tcgcaccgcg ggcggcggtg tgccgcgcca aagcccgcgg gtatttctct ataacgacaa
     1321 cGATATCtca ctatcccatc tagccgcggc aggcccagac ctccggcaag atgaccgccg
     1381 ccccgtcata tcgggcggcg gcaggatgta caacaagaac aaggagcaac ctgcgtgaat
     1441 aacgaagccc gctcagggtc gaccgaccct ggccaacgtc cgcgctaccg ccaggtggcc
     1501 atcgggcatc cccaggtgca ggtcagtcac gtcgacgacg tgctgcgcat gcaacctgtc
     1561 gagccactgg cgccgctgcc ggcgcgcctg ctcgagcgcc tggtgcattg ggcccaggtg
     1621 cgcccggaca ccactttcat cgcggcacgc caggcagacg gtgcctggcg ttcgatcagc
     1681 tacgtgcaga tgctcgccga tgtgcgcacc atcgccgcca acttgctagg actgggcctc
     1741 agtgccgagc gcccgctggc gctgctttcc ggcaacgaca tcgaacacct gcaaatcgcc
     1801 ctcggcgcca tgtatgccgg tattgcctat tgcccggtgt cgccggccta cgcgctgttg
     1861 tcgcaagact tcgccaagtt gcgccatgtc tgcgaggtgc tcacccccgg agtggtcttc
     1921 gtcagcgaca gccagccgtt ccagcgcgcc ttcgaggcgg tgctggacga ttcggtcggc
     1981 gtgatcagcg tgcgtggcca ggtcgcaggt cgcccccata taagcttAAG CTTggcactg
     2041 gccgtcgttt tacaacgtcg tgaccggaat tgccagctgg ggcgccctct ggtaaggttg
     2101 ggaagccctg caaacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     2161 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     2221 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     2281 caagaccgac ctgtccggtg ccctgaatga actccaagac gaggcagcgc ggctatcgtg
     2341 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     2401 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     2461 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     2521 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     2581 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     2641 actgttcgcc aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg tgacccatgg
     2701 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     2761 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     2821 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     2881 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgacgatgaa
     2941 catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg
     3001 aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg
     3061 catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa
     3121 atatcaagtt tctgaatttg attcgtccac aattaaaaat atctcttctg caaaaggcct
     3181 ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg
     3241 ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta
     3301 catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt
     3361 ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga
     3421 atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt
     3481 ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc
     3541 agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg
     3601 aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa
     3661 ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga
     3721 agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata
     3781 ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa
     3841 aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta
     3901 cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga
     3961 acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc
     4021 aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa
     4081 ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct
     4141 tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa
     4201 caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac
     4261 gtttgcgccg agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag
     4321 catccttgaa caaggacaat taacagttaa caaataatca gaccccgtag aaaagatcaa
     4381 aggatcttc     
//
