LOCUS       pIAR010_modABC_d        4526 bp DNA     circular SYN 16-SEP-2020
DEFINITION  Cloning vector pK18sB, complete sequence.
ACCESSION   MH166772
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Jayakody LN, Johnson CW, Whitham JM, Giannone RJ, Black BA, 
            Cleveland NS, Klingeman DM, Michener WE, Olstad JL, Vardon DR, Brown
            RC, Brown SD, Hettich RL, Guss AM, Beckham GT.
  TITLE     Thermochemical wastewater valorization via enhanced microbial 
            toxicity tolerance
  JOURNAL   Energy Environ Sci (2018) In press
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Johnson CW.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2018) National Bioenergy Center, National 
            Renewable Energy Laboratory, 15301 Denver West Parkway, Golden, CO 
            80401, USA
REFERENCE   3  (bases 1 to 4526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 22, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Energy 
            Environ Sci (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-APR-2018) National Bioenergy Center, National Renewable Energy 
            Laboratory, 15301 Denver West Parkway, Golden, CO 80401, USA"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /mol_type="other DNA"
                     /db_xref="taxon:2201328"
                     /organism="Cloning vector pK18sB"
     source          632..1401
                     /strain="KT2440"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:160488"
                     /organism="Pseudomonas putida KT2440"
     source          1408..2164
                     /strain="KT2440"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:160488"
                     /organism="Pseudomonas putida KT2440"
     rep_origin      1..595
                     /label=pMB1 replication origin
                     /note="pMB1 replication origin"
     primer_bind     509..528
                     /label=oIAR007
                     /note="Forward sequencing primer for targeting regions 
                     inserted at the EcoRI site"
     primer_bind     601..617
                     /note="special M13R binding site; M13R"
     primer_bind     602..625
                     /label=USTR primer overhang
                     /note="Where any USTR constructed with an EcoRI site can 
                     integrate into this plasmidPrimers that use this 
                     overhang:oIAR001"
     misc_feature    632..1401
                     /label=UsTR
     gene            632..1322
                     /gene="yrpB"
                     /locus_tag="PP_3827"
                     /label=yrpB
     CDS             632..1322
                     /codon_start=1
                     /transl_table=11
                     /gene="yrpB"
                     /locus_tag="PP_3827"
                     /product="2-nitropropane dioxygenase"
                     /EC_number="1.13.12.16"
                     /label=yrpB
                     /note="Evidence 1c : Function experimentally demonstratedin
                     the studied genus; PubMedId : 16682407, 10231533, 18305197;
                     Product type e : enzyme; Biologicalprocesses : Protect"
                     /protein_id="AAN69421.1"
                     /translation="VVSFHFGLPQAELLQRVKASGAKVLSSATTVEEAAWLERNGCDAI
                     IAMGYEAGGHRGMFLSDDITSQIGTFALVPQVADAVGVPVIAAGGIGDHRGLLAALALG
                     ASAVQIGTAYLFCPEAKVSPAHRQALDSAPASDTALTNLFTGRPARGINNRLMRELGPM
                     SELAPRFPLAGGALMPLRAITDPQGKSDFSNLWSGQALRLGRHMPAGELTREIAGKALA
                     VIGHQAF"
     primer_bind     632..652
                     /label=oIAR089
                     /note="UsTR annealing portion F"
     primer_bind     complement(929..938)
                     /label=DSTR primer plasmid overhang
                     /note="Where any DSTR constructed with a HindIII site can 
                     integrate into this plasmid"
     primer_bind     1377..1401
                     /label=overhang of oIAR091
     primer_bind     complement(1381..1401)
                     /label=oIAR090
                     /note="UsTR annealing portion R"
     misc_feature    1408..2164
                     /label=DsTR
     primer_bind     complement(1408..1432)
                     /label=overhang of oIAR090
     primer_bind     1408..1425
                     /label=oIAR091
                     /note="DsTR annealing portion"
     gene            1408..1413
                     /gene="modC"
                     /locus_tag="PP_3830"
                     /label=modC
     CDS             1408..1413
                     /codon_start=1
                     /transl_table=11
                     /gene="modC"
                     /locus_tag="PP_3830"
                     /product="Molybdenum import ATP-binding protein ModC"
                     /EC_number="3.6.3.29"
                     /label=modC
                     /protein_id="AAN69424.1"
                     /translation="G"
     gene            1471..2164
                     /locus_tag="PP_3831"
                     /label=PP_3831
     CDS             1471..2164
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="PP_3831"
                     /product="conserved protein of unknown function"
                     /label=PP_3831
                     /note="Evidence 4 : Homologs of previously reported genesof
                     unknown function"
                     /protein_id="AAN69425.1"
                     /translation="MLDCPLPRTLHYVDDSQPGLTRRRWRDRFIYLDADGQRVRDSETL
                     ARIAALVIPPAYTDVWICADPQGHLQATGRDARGRKQYRYHAQWRELRDQHKYGRMLAF
                     AQALPKLRTQLEAHLARPGLDREKVMALVVSLLDHTLIRIGNQRYLRDNRSYGLTTLRN
                     RHVQVKGSTIRFQFRGKRGVEHNVTLNDRRLANLLKRCMELPGQALFQYLDEDGQRHSV
                     GSSEVNQFL"
     primer_bind     2119..2128
                     /label=DSTR primer plasmid overhang
                     /note="Where any DSTR constructed with a HindIII site can 
                     integrate into this plasmid"
     primer_bind     complement(2144..2164)
                     /label=oIAR092
                     /note="DsTR annealing portion"
     primer_bind     complement(2171..2194)
                     /label=DSTR primer plasmid overhang
                     /note="Where any DSTR constructed with a HindIII site can 
                     integrate into this plasmid"
     primer_bind     complement(2174..2191)
                     /label=M13F
                     /note="M13F"
     regulatory      2201..2250
                     /label=NEOKAN promoter
                     /note="NEOKAN promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2224..2242)
                     /label=oIAR008
                     /note="Reverse sequencing primer for targeting regions 
                     inserted at the HindIII site"
     gene            2272..3066
                     /gene="nptII"
                     /label=nptII
     CDS             2272..3066
                     /codon_start=1
                     /transl_table=11
                     /gene="nptII"
                     /product="neomycin phosphotransferase"
                     /label=nptII
                     /protein_id="AWK60330.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     gene            3073..4494
                     /gene="sacB"
                     /label=sacB
     CDS             3073..4494
                     /codon_start=1
                     /transl_table=11
                     /gene="sacB"
                     /product="levan sucrase"
                     /label=sacB
                     /protein_id="AWK60331.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgcctcaca
      601 caggaaacag ctatgacatg attacgaatt cggtggtgag cttccatttc ggcctgccgc
      661 aggccgaact gctgcagcgg gtaaaggcca gcggtgccaa ggtactgtcc agcgccacga
      721 ccgtggaaga agcggcctgg ctggagcgca atggctgcga tgcgatcatc gccatggggt
      781 atgaagccgg tggccatcgc ggcatgttcc tcagcgatga catcaccagc cagataggca
      841 ccttcgcgct cgtaccgcag gtggccgatg ccgtcggcgt gccggtgatt gccgccggtg
      901 gtatcggcga ccaccgcggg ctgttggcgg cactggccct gggtgcctcg gcagtgcaga
      961 tcggcacggc ttacctgttc tgccccgagg ccaaggtgtc gccagcgcat cgccaggcgc
     1021 tggacagcgc gcctgccagc gacaccgccc tgaccaacct gttcaccggc cgcccggcgc
     1081 gcggcatcaa caatcgcctc atgcgcgagc tggggccgat gagcgagctt gcaccgcgct
     1141 tcccgctggc gggcggggca ttgatgcctt tacgggcgat caccgatccg cagggtaaga
     1201 gtgatttcag caatctgtgg tcggggcagg cgttacggct aggccggcac atgccggcgg
     1261 gtgagctgac ccgggagatt gccggtaagg cattggcagt gatcggtcac caggcattct
     1321 gacaggcagg catggcctct tcacggccaa tggcttatcg ctatatagtt aaggctataa
     1381 cgataacctc aaggagctgt cgatatcggt tgagcatcct cagcgcgcgt attgtccatt
     1441 gatcagtgac ctgatcgagg cccgccgccc atgcttgact gccccctgcc gcgcaccctg
     1501 cactacgttg acgacagtca gccgggcctg acccgtcggc gctggcgcga ccgtttcatc
     1561 tacctggatg ccgatggcca acgggtacgc gacagcgaaa cccttgcgcg catcgccgca
     1621 ttggtgatcc ccccggccta cacggatgtg tggatctgcg ccgatccgca aggccacctg
     1681 caagccaccg gccgcgatgc ccgtggccgc aagcagtacc gctaccacgc acagtggcgc
     1741 gaactgcgcg accagcacaa atacgggcgc atgcttgcgt tcgcccaagc cctgccaaag
     1801 ctgcgcacac agctggaagc ccacttggcg cggccagggc tggaccggga aaaagtcatg
     1861 gcgctggtgg tgagcctgct ggaccacacc ctgatccgca tcggcaacca gcgctacctg
     1921 cgcgataacc ggtcgtacgg attgaccacc ctgcgcaatc gccatgtgca ggtcaaaggc
     1981 agcactatcc gcttccagtt ccgcggcaag cgcggcgtcg aacacaacgt caccctcaac
     2041 gaccggcgct tggccaacct actcaaacgc tgcatggaac tgcctgggca ggcactgttt
     2101 cagtacctgg atgaagatgg ccagcgccat agtgtcggct ccagcgaggt caatcagttt
     2161 ctgcaagctt ggcactggcc gtcgttttac aacgtcgtga ccggaattgc cagctggggc
     2221 gccctctggt aaggttggga agccctgcaa acaggatgag gatcgtttcg catgattgaa
     2281 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2341 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2401 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaagacgag
     2461 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     2521 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     2581 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     2641 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     2701 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     2761 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga cggcgaggat
     2821 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     2881 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     2941 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3001 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3061 ttctgagcga cgatgaacat caaaaagttt gcaaaacaag caacagtatt aacctttact
     3121 accgcactgc tggcaggagg cgcaactcaa gcgtttgcga aagaaacgaa ccaaaagcca
     3181 tataaggaaa catacggcat ttcccatatt acacgccatg atatgctgca aatccctgaa
     3241 cagcaaaaaa atgaaaaata tcaagtttct gaatttgatt cgtccacaat taaaaatatc
     3301 tcttctgcaa aaggcctgga cgtttgggac agctggccat tacaaaacgc tgacggcact
     3361 gtcgcaaact atcacggcta ccacatcgtc tttgcattag ccggagatcc taaaaatgcg
     3421 gatgacacat cgatttacat gttctatcaa aaagtcggcg aaacttctat tgacagctgg
     3481 aaaaacgctg gccgcgtctt taaagacagc gacaaattcg atgcaaatga ttctatccta
     3541 aaagaccaaa cacaagaatg gtcaggttca gccacattta catctgacgg aaaaatccgt
     3601 ttattctaca ctgatttctc cggtaaacat tacggcaaac aaacactgac aactgcacaa
     3661 gttaacgtat cagcatcaga cagctctttg aacatcaacg gtgtagagga ttataaatca
     3721 atctttgacg gtgacggaaa aacgtatcaa aatgtacagc agttcatcga tgaaggcaac
     3781 tacagctcag gcgacaacca tacgctgaga gatcctcact acgtagaaga taaaggccac
     3841 aaatacttag tatttgaagc aaacactgga actgaagatg gctaccaagg cgaagaatct
     3901 ttatttaaca aagcatacta tggcaaaagc acatcattct tccgtcaaga aagtcaaaaa
     3961 cttctgcaaa gcgataaaaa acgcacggct gagttagcaa acggcgctct cggtatgatt
     4021 gagctaaacg atgattacac actgaaaaaa gtgatgaaac cgctgattgc atctaacaca
     4081 gtaacagatg aaattgaacg cgcgaacgtc tttaaaatga acggcaaatg gtacctgttc
     4141 actgactccc gcggatcaaa aatgacgatt gacggcatta cgtctaacga tatttacatg
     4201 cttggttatg tttctaattc tttaactggc ccatacaagc cgctgaacaa aactggcctt
     4261 gtgttaaaaa tggatcttga tcctaacgat gtaaccttta cttactcaca cttcgctgta
     4321 cctcaagcga aaggaaacaa tgtcgtgatt acaagctata tgacaaacag aggattctac
     4381 gcagacaaac aatcaacgtt tgcgccgagc ttcctgctga acatcaaagg caagaaaaca
     4441 tctgttgtca aagacagcat ccttgaacaa ggacaattaa cagttaacaa ataatcagac
     4501 cccgtagaaa agatcaaagg atcttc
//
