LOCUS       Exported                4940 bp ds-DNA     circular SYN 24-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 16-11-2019 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        566..1065
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1085..1684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             1691..1747
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1748..2467
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    2495..2719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3043..3059)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3242..3830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4001..4861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLRNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERKRQIAEIGAS
                     LIKHW"
     promoter        complement(join(4862..4940,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg aaccacgcaa tgcgtctcga tccgcagtgt cttgcgtctc
      181 taagctttac actagtgggc ataagtattt agacaccatt agcttgcaac tgccgggcta
      241 tgcttttcat agtacagtca aataaataag taaactattg atgataaaac aaagagtgca
      301 attacagtta cacgcgagag gtatgttttg ccgtttacct tttcaattat ccttctgctg
      361 ataagaaagc tagacagaca acgatttggg cggtggcaaa agtatttaga catcacattt
      421 aattagttcg cattgatctg ttgttcgaag aatatcaaat ttttgacttt tttagatcat
      481 ttgaacattt gttcttgata acgtgttact acctactttt atttcattca taaatttgtt
      541 aaacaccggt gctagcaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
      601 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
      661 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
      721 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
      781 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
      841 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
      901 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
      961 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     1021 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaacgc
     1081 caccatgggg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     1141 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     1201 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     1261 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     1321 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     1381 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1441 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1501 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1561 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1621 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1681 tgccatcgat gccactaact tctccctgtt gaaacaagca ggggatgtcg aagagaatcc
     1741 cgggccaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1801 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1861 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1921 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1981 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2041 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2101 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     2161 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     2221 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     2281 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     2341 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     2401 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2461 caagtaatta attaagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2701 gatgcggtgg gctctatggc ttctgaggcg cgccctcgag tttgtcataa ttttttagtt
     2761 gacttttttt tttcaattta taacatttaa ataatttgtc caaatactta tgccactgct
     2821 aatttgtaaa aaagtaattt ttgttaaatc aacaatgaag ttatccatat gtttatgtta
     2881 cctaatttaa ctacgatagt ctacctattt actaaaatta ttaaacaatt ttgctttaag
     2941 taaacaatga gttacaaaca aatatattga atatatttct tgtccaaata cttatgccca
     3001 ctagtgtagg gcccagagac ggagtcactg ccaaccgaga cggtcatagc tgtttcctgt
     3061 gtgccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3121 cagctcactc aaaggcggta atacggttac ccacagaatc aggggataac gcaggaaaga
     3181 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3241 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3301 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3361 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3421 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3481 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3541 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3601 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3661 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3721 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4021 aggcacctat ctcagcgatc tgtctctttc gttcatccat agttgcctga ctccccgtcg
     4081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca ataataccgc
     4141 gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag
     4321 gcatcgtggt atcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4381 caaggcgagt tacatgatcc cccatgttgc gcaaaaaagc ggttagctcc ttcggtcctc
     4441 cgatcgttgt cagaagtaag ttggccgccg tgttatcact catggttatg gcagcactac
     4501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4861 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4921 acatatttga atgtatttag
//
