LOCUS       Exported                7324 bp ds-DNA     circular SYN 26-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   fdfasfdaffdagdga
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 27, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3501
                     /codon_start=1
                     /label=AgaP12
                     /translation="MPVPCSVSLCGNNPRNVKKRALEISFHIFPNYDPLRHAWVQFCNR
                     EENWEPSKRDVICSAHFQESDFQMPESGIVGGKVLRRTLFPNGKFLLAYFPNDDTTNIL
                     CFCWLLAIPSIHSVLSSEIENREEVSSENFVPSTSDTLLVSDVSNIPQNTSTAFLKENI
                     KLREANKRLKATLSHIASENYQLKKKVQDLEKELNSIKNSSIPPSDLIPKMKNMLKSTL
                     TSNQVDLIIGEKKRVRWTKEEISRALTLRYFGKKAYDYVKDDLKIYLPSPSTLQKYART
                     FKLREGILEDVLVFMGNFVSSLSSRDAECILSFDEMKIKNVMEYDPSADEVIGPYNYIQ
                     VVMARALFKNWKQPIYIGFDKKMTKEILMNIIIKLDEKKINVAGIVSDNCSSNISCWRD
                     LGAHDYTKPYFEHPITKKNIYVSPDAPHLLKLLRNWFIDHGFVFNGTIVTAQPLRDLVE
                     GRLGAEITPLFKLNTGHLELSSQERQNVRRAAELLSRTTAVSLRRYMPKEKELADIIEM
                     VDLWFSVSNSFHPNAKLHYKRSYTASEDQLKALDDMFNFIANMIVVGKKNMQVFQKSIL
                     MQINSLKMLFVDMKAKHDINYLSTHKLNQDLLENFFSQLRQKGGTYDHPSPLNCIYRIR
                     LMILGKSPSVLKSVTDNENNKKNIEPDVFVTSADFENETDQLNEICVSQEHYVSATIFH
                     EAEIVPSLLDFDATDADDEFSATNFDSLFILNEQEKDGLEYIIGYIGRKFRDKYPYLGQ
                     YTCNLTEDHCYSQPQSYVEHISAGGLYKPSASFLDQGLKMEEIFQRTHKDGKLIQSKRI
                     VNTLTDLLQTHFPDFPIEILKTFAKLRIIMRMRFFNIKKEEIRRNKKRKTPMERQAAKK
                     FRRIVNAAARACI"
     promoter        complement(3512..3530)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3556..3780
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3826..4254
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4268..4597
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4448..4583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4763..4884
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4933..4949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4957..4973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4981..5011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5026..5047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5335..5923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6094..6954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6955..7059)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgc ctgtgccttg tagcgttagc ctgtgtggca acaaccccag aaacgtgaag
      901 aagagagccc tggagatcag cttccacatc ttccccaact acgaccctct gagacacgct
      961 tgggtgcagt tctgcaacag agaggagaac tgggagccca gcaagaggga cgtgatctgc
     1021 agcgcccact tccaggaaag cgacttccag atgcccgaaa gcggaattgt gggcggaaag
     1081 gtgctgagaa gaaccctgtt ccccaacggc aaattcctgc tggcctactt ccctaacgac
     1141 gacaccacca acatcctgtg cttctgctgg cttctggcca ttcccagcat tcacagcgtt
     1201 ctgagcagcg agatcgagaa cagagaggag gtgagcagcg aaaacttcgt gcccagcaca
     1261 agcgatactc tgctggtgag cgacgtgagc aacatccccc agaataccag caccgccttc
     1321 ctgaaggaga acatcaagct gagagaggcc aacaagagac tgaaggccac cctgagccat
     1381 attgccagcg agaactacca gctgaagaag aaggtgcagg acctggagaa ggagctgaac
     1441 agcatcaaga acagcagcat ccctcccagc gacctgattc ccaagatgaa gaacatgctg
     1501 aagagcaccc tgaccagcaa tcaggtggac ctgatcatcg gcgagaagaa gagagtgaga
     1561 tggaccaagg aggagatcag cagagccctg accctgagat acttcggcaa gaaggcctac
     1621 gactacgtga aggacgacct gaagatctac ctgcctagcc ctagcaccct gcagaaatac
     1681 gccagaacct tcaagctgag agagggcatc ctggaagacg tgctggtgtt catgggcaac
     1741 ttcgtgagca gcctgagcag cagggacgct gagtgcatcc tgagcttcga cgagatgaag
     1801 atcaagaacg tgatggagta cgaccctagc gccgacgaag tgattggacc ctacaactac
     1861 atccaggtgg tgatggccag agccctgttc aagaactgga agcagcccat ctacatcggc
     1921 ttcgacaaga agatgaccaa ggagatcctg atgaacatca tcatcaagct ggacgagaag
     1981 aagatcaacg tggccggcat cgtgtctgat aactgcagca gcaacatcag ctgctggaga
     2041 gatctgggag cccacgatta caccaagccc tacttcgagc accccatcac caagaagaac
     2101 atctacgtga gccctgacgc tcctcacctg ctgaaactgc tgagaaactg gttcatcgac
     2161 cacggcttcg tgttcaacgg caccatcgtg acagctcagc ccctgagaga tctggttgag
     2221 ggcagactgg gagctgaaat cacccccctg ttcaagctga ataccggcca cctggaactt
     2281 tctagccagg agagacagaa cgtgagaaga gctgccgagc tgctgagcag aacaaccgct
     2341 gtgagcctga gaagatacat gcccaaggag aaggagctgg ccgacatcat cgagatggtg
     2401 gacctgtggt tcagcgtgag caacagcttt caccccaacg ccaagctgca ctacaagaga
     2461 agctacaccg ccagcgagga ccaacttaag gctctggacg acatgttcaa cttcatcgcc
     2521 aacatgatcg tggtgggcaa gaagaacatg caggtgttcc agaagagcat cctgatgcag
     2581 atcaacagcc tgaagatgct gttcgtggac atgaaggcca agcacgacat caactacctg
     2641 agcacccaca agctgaacca ggacctgctg gagaacttct tcagccagct gagacaaaag
     2701 ggcggcacct atgatcatcc tagccccctg aactgcatct acagaatcag actgatgatc
     2761 ctgggcaaaa gccccagcgt gctgaaaagc gtgaccgaca acgagaacaa caagaagaac
     2821 atcgagcccg acgtgtttgt tacaagcgcc gacttcgaga atgagacaga ccagctgaac
     2881 gagatctgcg tgagccagga gcattatgtg agcgccacca tctttcatga ggccgagatt
     2941 gtgcctagcc tgctggactt tgacgctaca gacgccgacg atgagttcag cgccaccaac
     3001 ttcgacagcc tgttcatcct gaacgagcag gagaaagacg gcctggagta catcatcggc
     3061 tacatcggca gaaagttcag agacaagtac ccctacctgg gccagtatac ctgcaacctg
     3121 accgaggacc actgttacag ccagcctcag agctacgtgg agcacatttc tgctggcggc
     3181 ctgtataaac ctagcgccag cttcctggat cagggcctga agatggagga gatcttccag
     3241 agaacccaca aggacggcaa gctgatccag agcaagagaa tcgtgaacac cctgaccgac
     3301 ctgctgcaaa cccacttccc cgatttcccc atcgagatcc tgaagacctt cgccaagctg
     3361 agaatcatca tgagaatgcg gttcttcaac atcaagaagg aggagatcag aagaaacaag
     3421 aagagaaaga cccccatgga gagacaagcc gccaagaagt tcagaagaat cgtgaacgcg
     3481 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc
     3541 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     3601 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     3661 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     3721 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     3781 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     3841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4261 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4321 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4381 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4501 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4561 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgaaa tgaccgacca
     4621 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     4681 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4741 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4861 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4921 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4981 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5041 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5881 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5941 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6001 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6061 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6121 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6181 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6241 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6301 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6361 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6421 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6481 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6541 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6601 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6661 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6721 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6781 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6841 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6901 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6961 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7021 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7081 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     7141 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     7201 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7261 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     7321 cgtt
//
