LOCUS       Exported                5815 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..1965
                     /codon_start=1
                     /label=POGO
                     /translation="MGKTKRVVGLTLKEKLQIIELVTNKVDKKEICAKFKCDRSTVNRI
                     LQKTNEIHEAVAASGLKRKRQRKGAHDLVEEALYIWFGQQESKNVILDRHVILAKAKEF
                     CQKFNDAFEPDASWLWRWRKRHNIKYGKIHGETATNDSVSANEYKNDILPGLLKGYNPE
                     DIFNADETALFYKAMPNATFFTCGKQLNGQKSQRVRLTLLFICNATGTYKKTFVIGRSK
                     SPRCFKNANVPIPYYANKKAWMTKDLWRKIMTGFDEEMKKQNRKILLFIDNATSHTTVK
                     DFENIKLCFMPPNATALLQPLDQGIIHSFKLEYRRILVKQQLIAVNCGKSTVEFLKSLS
                     LLDALYFVNQGWKNVKMLTIQNCFKKVRWDYY"
     promoter        complement(2003..2021)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2047..2271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2317..2745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2759..3088
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2939..3074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3254..3375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3424..3440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3448..3464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3472..3502)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3517..3538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3826..4414)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4585..5445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5446..5550)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg gcaaaaccaa acgcgtggtg ggcctgaccc tgaaagaaaa gctgcagatt
      901 attgagctgg tgaccaacaa agtggacaag aaagagattt gcgcgaagtt caaatgcgac
      961 cgcagcaccg tgaatcgcat tctgcagaaa accaacgaga ttcatgaggc ggttgcggca
     1021 tcaggtctga aacgtaaacg tcagcgcaaa ggcgcacatg atctggtgga ggaagccctg
     1081 tatatttggt ttggccagca ggagagcaaa aacgtgatcc tggaccgcca tgtgattctg
     1141 gccaaagcca aagagttctg ccagaagttt aacgatgcgt ttgaacctga tgcgagctgg
     1201 ttatggcgtt ggcgtaaacg ccacaacatc aagtatggca agattcatgg cgaaaccgcc
     1261 accaatgata gcgtgagcgc gaacgagtat aaaaacgata ttctgccggg cctgctgaaa
     1321 ggctataacc ctgaggatat cttcaacgcg gatgaaaccg cgctgttcta taaagccatg
     1381 ccgaacgcga ccttttttac ctgcggcaaa cagctgaacg gccagaagag ccagcgtgtt
     1441 cgtctgaccc tgctgtttat ctgcaacgcg accggcacct ataaaaagac ctttgtgatt
     1501 ggccgcagca aaagccctcg ctgctttaaa aacgccaacg tgccgattcc gtattacgcg
     1561 aacaagaaag cgtggatgac caaggatctg tggcgcaaaa tcatgaccgg ctttgacgaa
     1621 gaaatgaaga agcagaaccg caaaatcctg ctgtttatcg acaacgcgac cagccatacc
     1681 accgtgaaag atttcgagaa catcaagctg tgctttatgc cgcctaacgc cacagcatta
     1741 ctgcaacctt tagatcaggg cattatccac agcttcaaac tggaataccg ccgcattctg
     1801 gtgaaacagc agctgatcgc ggtgaactgc ggcaaaagca ccgtggaatt tctgaaaagc
     1861 ctgagcctgc tggatgccct gtatttcgtg aaccagggct ggaaaaacgt gaaaatgctg
     1921 accattcaga actgctttaa gaaggtgcgc tgggactact actaatgagc ggccgctcga
     1981 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     2041 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2101 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2161 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2221 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     2281 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     2341 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     2401 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     2461 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     2521 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     2581 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     2641 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2701 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     2761 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2821 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     2881 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     2941 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3001 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3061 cttttttgga ggcctaggct tttgcaaaaa gctccccgaa atgaccgacc aagcgacgcc
     3121 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3181 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3241 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3361 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     3421 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     3481 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     3541 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3601 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     3661 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     3721 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3781 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3841 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3901 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3961 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4021 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4081 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4141 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4201 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4261 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     4321 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     4381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     4441 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     4501 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4561 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4621 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4681 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4741 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4801 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4861 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4921 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4981 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5041 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5101 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5161 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5221 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5281 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5341 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5401 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5461 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5521 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     5581 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     5641 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     5701 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     5761 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
