LOCUS       Exported                6631 bp ds-DNA     circular SYN 08-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2781
                     /codon_start=1
                     /label=HOBO
                     /translation="MAPYIMIVEFLCLWSSVSAVNCPFFVFYDAITSLLGFSIIWKPKE
                     KVTIMAEAADFVKNKINNGTYSVANKHKGKSVIWSILCDILKEDETVLDGWLFCRQCQK
                     VLKFLHKNTSNLSRHKCCLTLRRPTELKIVSENDKKVAIEKCTQWVVQDCRPFSAVTGA
                     GFKNLVKFFLQIGAIYGEQVDVDDLLPDPTTLSRKAKSDAEEKRSLISSEIKKAVDSGR
                     ASATVDMWTDQYVQRNFLGITFHYEKEFKLCDMILGLKSMNFQKSTAENILMKIKGLFS
                     EFNVENIDNVKFVTDRGANIKKALEGNTRLNCSSHLLSNVLEKSFNEANELKKIVKSCK
                     KIVKYCKKSNLQHTLETTLKSACPTRWNSNYKMMKSILDNWRSVDKILGEADIHVDFNK
                     SSLKVVVDILGDFERIFKKLQTSSSPSICFVLPSISKILELCEPNILDLSAAALLKERI
                     LENIRKIWMANLSIWHKAAFFLYPPAAHLQEEDILEIKVFCISQIQVPISYTLSLESTE
                     TPRTPETPETPETPETPETPETPETPETPETPESLESPNLFPKKNKTISSENEFFFPKP
                     VTESNSNFNESPLDEIERYIRQRVPLSQNFEVIEWWKNNANLYPQLSKLALKLLSIPAS
                     SAELKECFP"
     promoter        complement(2819..2837)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2863..3087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3133..3561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3575..3904
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3755..3890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4070..4191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4240..4256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4264..4280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4288..4318)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4333..4354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4642..5230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5401..6261)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6262..6366)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg cgccgtacat tatgatcgtg gaatttctgt gcctgtggag ctcagttagc
      901 gcagttaact gccctttctt tgtgttctac gacgccatta ccagcctgct gggctttagc
      961 attatctgga agccgaagga aaaggtgacc attatggcgg aagccgccga ctttgtgaaa
     1021 aacaagatca acaacggcac ctatagcgtg gcgaacaaac ataaaggcaa gagcgtgatt
     1081 tggagcattc tgtgcgacat cctgaaggaa gacgaaaccg tgttagatgg ctggctgttt
     1141 tgtcgccagt gccagaaagt gctgaaattc ctgcataaaa acaccagcaa cctgagccgc
     1201 cataaatgct gcctgacctt acgtcgcccg accgagttaa aaatcgtgag cgagaacgat
     1261 aaaaaagtgg cgattgagaa gtgcacccag tgggtggtgc aagattgccg tccttttagc
     1321 gcagttaccg gcgcaggctt taagaacctg gtgaagttct ttctgcagat cggcgccatt
     1381 tatggcgagc aggtggatgt ggatgatctg ctgccggatc ctactacctt aagccgcaaa
     1441 gcgaaaagcg acgcggaaga aaagcgcagc ctgattagca gcgaaattaa gaaggccgtg
     1501 gattcaggtc gtgcgagcgc aaccgttgat atgtggaccg atcagtatgt gcagcgcaac
     1561 tttctgggca ttaccttcca ttacgagaaa gagttcaagc tgtgcgatat gatcctgggc
     1621 ctgaagagca tgaactttca gaaaagcacc gccgaaaaca ttctgatgaa gattaagggc
     1681 ctgttcagcg agtttaacgt ggaaaacatc gacaacgtga aatttgtgac cgatcgcggc
     1741 gccaacatca aaaaagcgct ggaaggcaac actcgcctga attgcagcag ccatctgctg
     1801 agcaacgtgc tggaaaagag cttcaacgaa gcgaacgaac tgaagaaaat cgtgaagagc
     1861 tgcaaaaaga tcgtgaagta ctgcaagaaa agcaacctgc agcataccct ggaaaccacc
     1921 ctgaaaagcg catgccctac ccgttggaac agcaactaca aaatgatgaa gagcatcctg
     1981 gataactggc gcagcgtgga taaaattctg ggcgaagccg atattcatgt ggacttcaac
     2041 aaaagcagcc tgaaagtggt ggtggatatc ctgggcgatt tcgaacgcat ctttaagaaa
     2101 ctgcagacca gcagcagccc gagcatttgt ttcgtgctgc cgagcatcag caagatcctg
     2161 gaactgtgcg agccgaacat tctggattta agcgcggcgg cattattaaa agagcgcatc
     2221 ctggaaaaca ttcgcaagat ttggatggcg aacctgagca tttggcataa agcggcgttt
     2281 ttcctgtatc ctccggcggc acacttacag gaggaagata ttctggagat caaggtgttt
     2341 tgcatcagcc agattcaggt gccgattagc tataccctga gcctggaaag caccgaaacc
     2401 cctcgtactc cggaaacccc tgaaacccct gaaaccccgg aaacccctga aactccggaa
     2461 acgcctgaaa ctcctgaaac ccctgaaacc ccggaaagcc tggaaagccc taacctgttt
     2521 ccgaaaaaga acaagaccat cagcagcgaa aacgagttct tctttccgaa gccggtgacc
     2581 gaaagcaaca gcaactttaa cgagagccct ctggatgaaa ttgagcgcta cattcgccag
     2641 cgcgtgcctt taagccagaa ctttgaagtg atcgagtggt ggaaaaacaa cgcgaacctg
     2701 tatcctcagc tgagcaagct ggcgctgaaa ctgttaagca ttccggcgag cagcgccgaa
     2761 ctgaaagaat gcttcccgtg atgagcggcc gctcgagcat gcatctagag ggccctattc
     2821 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     2881 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2941 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3001 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3061 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3121 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3181 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3241 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3301 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3361 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3421 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3481 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3541 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3601 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3721 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3781 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3841 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3901 caaaaagctc cccgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     3961 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     4021 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     4081 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4201 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     4261 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     4321 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     4381 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     4441 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4501 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4561 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4621 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4681 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4741 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4801 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4861 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4921 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4981 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5041 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5101 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5161 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5221 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5281 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5341 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5401 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5461 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5521 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5581 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5641 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5701 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5761 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5821 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5881 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5941 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6001 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     6061 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6121 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6181 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6241 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6301 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6361 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     6421 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     6481 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     6541 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     6601 cttcgcgatg tacgggccag atatacgcgt t
//
