LOCUS       Exported                6535 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="CMV enhancer;human cytomegalovirus immediate early 
                     enhancer;color: #ffffff"
     promoter        381..584
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 
                     early promoter;color: #ffffff; direction: RIGHT"
     promoter        629..647
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="T7 promoter;promoter for bacteriophage T7 RNA 
                     polymerase;color: #ffffff; direction: RIGHT"
     promoter        757..775
                     /locus_tag="T7 promoter(1)"
                     /label=T7 promoter(1) T7 promoter
                     /label=T7 promoter(1)
                     /note="T7 promoter;promoter for bacteriophage T7 RNA 
                     polymerase;color: #ffffff; direction: RIGHT"
     CDS             847..2685
                     /codon_start=1
                     /label=22-hAT-3_XT
                     /translation="MTSSQPAVKRKIDDEHRQFQEKWEMQYFFVEHRGIPTCLICAEKV
                     AVHKEYNLKRHYSTKHAEECAKYQGDERAKRVASLKACLMRQQDFFKKATKENVASVQA
                     SYMVSEMIAKAGKPFTEGEFVKKCMLQVASIICPEKKGQFSKISLSANTVAERISDMSS
                     DIYHQLCEKAKCFDAYSVALDESTDITGTAQLTIYVRGVDCNFELTEELLTIIPMHGQT
                     TANEIFHHLCDAIENAGLPWKRFVGIITDGAPSMTGRKNGLVALVKKKLEEEGIEEEAI
                     ALHCIIHQQALCSKCLPCDNVMSVVVKCVNQIRSRGLTHRRFRAFLEEMGSEYGDVLYF
                     TEVRWLSRGNVLKRFFELREEVKAFMEKNGKAVSELSDHKWLMDLAFLVDITQRLNVLN
                     KMLQGQGQLVSAAYDNVRAFSTKLVLWKSQLSQTNLCHFPACKELVDAGIPFSGEKYVD
                     AIFKLEKEFDHRFADFKTHRATFQIFVDPFSFDVQDAPPVLQMELIDLQCNSDIKAKFR
                     EMSGKADTHVQFLRELPPSFPELSRMFKRTMCLFGSTYLCEKLFSTLNFNKSKYRSRLN
                     DDHLQAILRVSTASSLKPNVVQICEKKRCQVSGSKE"
     promoter        complement(2723..2741)
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="SP6 promoter;promoter for bacteriophage SP6 RNA 
                     polymerase;color: #ffffff; direction: LEFT"
     polyA_signal    2767..2991
                     /locus_tag="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="bGH poly(A) signal;bovine growth hormone 
                     polyadenylation signal;color: #a6acb3"
     rep_origin      3037..3465
                     /direction=RIGHT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;color: #ffff00"
     promoter        3479..3808
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 promoter;SV40 enhancer and early 
                     promoter;color: #ffffff; direction: RIGHT"
     rep_origin      3659..3794
                     /locus_tag="SV40 ori"
                     /label=SV40 ori
                     /note="SV40 ori;SV40 origin of replication;color: #ffff00"
     polyA_signal    3974..4095
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal;SV40 polyadenylation 
                     signal;color: #a6acb3"
     primer_bind     complement(4144..4160)
                     /locus_tag="M13 rev"
                     /label=M13 rev
                     /note="M13 rev;common sequencing primer, one of multiple 
                     similar variants;color: #a020f0; direction: LEFT"
     protein_bind    4168..4184
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator;The lac repressor binds to the lac 
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).;color: 
                     #31849b"
     promoter        complement(4192..4222)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="lac promoter;promoter for the E. coli lac 
                     operon;This reverse directional feature has 3 segments: 
                     ???1:?4138?..?4144?/?#ffffff?/?-10 
                     ???2:?4145?..?4162?/?#ffffff 
                     ???3:?4163?..?4168?/?#ffffff?/?-35"
     protein_bind    4237..4258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4546..5134)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="ori;high-copy-number colE1/pMB1/pBR322/pUC origin of
                     replication;color: #ffff00"
     CDS             complement(5305..6165)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=bla
                     /label=AmpR
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: ???1:?5251?..?6042?/?#ccffcc 
                     ???2:?6043?..?6111?/?#ccffcc?/?signal?sequence Cleavage 
                     site after base 6042"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6166..6270)
                     /gene="bla"
                     /locus_tag="AmpR Promoter"
                     /label=bla AmpR promoter
                     /label=AmpR Promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga ccagctcaca acctgcggtg aaacgcaaaa tcgatgatga acatcgccag
      901 tttcaggaaa agtgggagat gcagtatttc tttgtggaac atcgcggcat tccgacctgt
      961 ctgatttgcg ccgagaaagt ggccgtgcat aaggagtata acctgaagcg ccactacagc
     1021 accaagcatg cggaagaatg cgccaaatat cagggcgatg aacgcgcaaa acgtgtggca
     1081 agcctgaaag catgcctgat gcgccagcag gatttcttca aaaaagcgac caaggaaaac
     1141 gtggcaagcg tgcaggcgag ctatatggtg agcgaaatga ttgcgaaagc cggcaaaccg
     1201 tttaccgaag gcgagttcgt gaagaaatgc atgctgcagg tggccagcat tatttgcccg
     1261 gagaagaagg gccagtttag caagatcagc ctgagcgcga atactgtggc ggaacgcatt
     1321 agcgatatga gcagcgacat ttatcatcag ctgtgcgaaa aagcgaagtg cttcgatgcg
     1381 tatagcgtgg cgctggatga aagcaccgat attaccggta ccgcgcagct gaccatatat
     1441 gtgcgcggcg tggattgcaa ctttgaactg accgaagaac tgctgaccat tattcctatg
     1501 cacggccaga ctaccgcgaa cgagattttt catcacctgt gcgatgccat tgaaaacgcc
     1561 ggcctgcctt ggaaacgctt tgtgggcatc ataaccgatg gtgcgcctag catgaccggt
     1621 cgcaaaaatg gcttagtggc gctggtgaag aaaaaactgg aggaagaagg cattgaggaa
     1681 gaagcgattg cgctgcattg cattattcac cagcaggcgc tgtgtagcaa atgcctgcct
     1741 tgcgataacg tgatgagcgt ggtggtgaaa tgcgtgaacc agatccgtag ccgcggctta
     1801 acccatcgtc gctttcgtgc cttcctggag gaaatgggca gcgaatatgg cgatgtgctg
     1861 tattttaccg aggtgcgctg gttatcacgt ggcaacgtgc tgaaacgctt ttttgaactg
     1921 cgcgaagaag tgaaagcgtt catggaaaaa aacggcaaag cggtgagcga gctgagcgat
     1981 cataaatggc tgatggacct ggcctttctg gtggacatta cccagcgcct gaacgtgctg
     2041 aacaaaatgc tgcagggcca aggccaactg gttagcgcgg cgtatgataa cgtgcgcgcg
     2101 tttagcacca aactggtgct gtggaaaagc cagctgagcc agaccaacct gtgtcatttc
     2161 ccggcctgca aagagttagt ggatgccggt attccgttca gcggcgaaaa gtatgtggat
     2221 gccattttca aactggagaa ggaatttgac caccgcttcg ccgattttaa aacccaccgc
     2281 gcgaccttcc agatctttgt ggatccgttt agctttgatg tgcaggatgc gcctccggtt
     2341 ctgcagatgg aactgatcga tctgcaatgc aacagcgata tcaaggcgaa gtttcgcgaa
     2401 atgagcggca aggcggatac ccatgtgcag tttctgcgcg aactgcctcc gagctttcct
     2461 gaactgagcc gcatgtttaa acgcaccatg tgcctgtttg gcagcaccta tctgtgcgaa
     2521 aagctgttta gcaccctgaa cttcaacaaa agcaagtacc gcagccgcct gaacgatgac
     2581 catctgcagg ccattctgcg tgttagcacc gcaagcagcc tgaaaccgaa cgtggtgcag
     2641 atctgcgaaa aaaaacgctg ccaggtgagc ggcagcaaag aataatgagc ggccgctcga
     2701 gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     2761 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2821 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2881 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2941 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3001 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3061 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3121 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3181 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3241 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3301 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3361 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3421 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     3481 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3541 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3661 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3721 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3781 cttttttgga ggcctaggct tttgcaaaaa gctccccgaa atgaccgacc aagcgacgcc
     3841 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     3901 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     3961 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4081 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     4141 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     4381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     4741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     4801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     4861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     4921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     4981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     5221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     5461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     5521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     5581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     5641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     5701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     5761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6301 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
     6361 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     6421 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     6481 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
