LOCUS       Exported                7378 bp ds-DNA     circular SYN 26-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   fdfasfdaffdagdga
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 27, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3555
                     /codon_start=1
                     /label=P1_AG
                     /translation="MSSNYKCCVAFCKNNRYNVKKVGVNVCFHKFPEGKETKQKWIAFC
                     QREISWIPSSSNVVCSQHFLPSDYQLSSSHNTKRGANWLNPEAVPSILLPQDTGLNLSQ
                     FNEHQQNNNNDESSRAELQGATSSHLLPDENDLILIGKENVCKKCKGLQQKIFNLEAKL
                     NELEERNKQLVTINNKLSDTLKEVNEKEKEHLKKLEELDKATKKIKEEWPRANFVQNMK
                     DSLKGSLSSNQIDLILGIKKTVRWTKEELSLAFTLRYFSQRAYRYIGDDMKIPVPVPRT
                     LQRYSSKIDLKQGILEDILKFIGSYSQTLKPMDRECVLSFDEMKVSRVLEYDPSADEIV
                     GPFNYLQVVMMRGLFKQWKQPIFIGFDTKMTKDIIIEIISRLSEKDINVVAIISDNCQT
                     NIGCWKELGARDDVEKPYFPHPKTNKNVYVIPDTPHLLKLLRNWLLDHGFEYNGQLIET
                     TNLLRMVAKRMESEMTPLFKLTTSHIDMTPQERQNVRRAAELLSRTTAVALRTYFPDDD
                     NAKILANFIEKVDVWFSISNSYTPFAKLDFKKAYTASDDQVRALTDMYDIISNMTIPGK
                     NGLQIFQRSIMMQIKSLQMVFADMKLKHDAKFICTHKLNQDVLENFFSQLRQIGGVYDH
                     PSPLSCIYRIRLMILGKTPTILHNQTTTVEAANCEQDEFITTTESNVAGARSNDVFISA
                     SMFEKAEITPELPDVKAMEEANNFVSQDVSSLSSVSDTSIELPHQEADGLSYILGYLAK
                     KHHSQFSHLNLGEHTFKTRIDHNYCQPPTFVHHLSCGGLIEPSDEFLNLGKKMEKIFLK
                     MNPDGGLLKGERIVDRITNKIKRHLTELPVEIIRSFAKQRVIVRMRYLNLKATAEQLNK
                     MKHKKRKFVTENTKAAKKMKKIINAAARACI"
     promoter        complement(3566..3584)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3610..3834
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3880..4308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4322..4651
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4502..4637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4817..4938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4987..5003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5011..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5035..5065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5080..5101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5389..5977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6148..7008)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7009..7113)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcagcaacta caagtgctgc gtggccttct gcaagaacaa cagatacaac
      901 gtgaagaagg tgggcgtgaa cgtgtgcttc cacaagttcc ccgagggcaa ggagacaaag
      961 cagaagtgga tcgccttctg ccagagagag atcagctgga tccccagcag cagcaatgtt
     1021 gtgtgcagcc agcactttct gcctagcgac taccaactga gcagcagcca caacacaaag
     1081 agaggcgcca actggcttaa tcctgaggct gtgccctcta ttctgctgcc tcaggacaca
     1141 ggactgaacc tgagccagtt caacgagcac cagcagaaca acaacaacga cgagagcagc
     1201 agagccgaac tgcaaggagc tacaagcagc catctgctgc ctgacgagaa cgacctgatc
     1261 ctgatcggca aggagaacgt gtgcaagaag tgcaagggcc tgcagcagaa gatcttcaac
     1321 ctggaggcca agctgaatga gctggaggag agaaacaagc agctggtgac catcaacaac
     1381 aagctgagcg acaccctgaa ggaggtgaac gagaaggaga aggagcacct gaagaagctg
     1441 gaggagctgg acaaggccac caagaagatc aaggaggagt ggcccagagc taacttcgtg
     1501 cagaacatga aggacagcct gaagggaagc ctgagcagca accagatcga cctgatcctg
     1561 ggcatcaaga agaccgtgag atggaccaag gaggagctga gcctggcttt caccctgaga
     1621 tacttcagcc agagagccta cagatacatc ggcgacgaca tgaagattcc cgtgcccgtg
     1681 cctagaaccc tgcagagata cagcagcaag atcgacctga agcagggcat cctggaggac
     1741 atcctgaagt tcatcggcag ctacagccaa accctgaagc ccatggacag agagtgcgtg
     1801 ctgagcttcg acgagatgaa ggtgagcaga gtgctggaat atgaccccag cgccgatgaa
     1861 attgtgggcc ccttcaacta tctgcaggtg gtgatgatga gaggcctgtt caaacagtgg
     1921 aagcagccca tcttcatcgg cttcgacacc aagatgacca aggacatcat catcgagatc
     1981 atcagcagac tgagcgagaa ggacatcaac gtggtggcca tcatcagcga caactgccag
     2041 accaacatcg gctgctggaa agaactgggc gccagagatg atgtggagaa gccctatttc
     2101 ccccacccca agaccaacaa gaacgtgtac gtgatccccg acacccctca tcttctgaag
     2161 ctgctgagaa actggcttct ggaccacggc ttcgagtata acggccagct gatcgagaca
     2221 accaacctgc tgagaatggt ggccaagaga atggagagcg agatgacccc cctgttcaag
     2281 cttaccacca gccacattga catgaccccc caggagagac agaacgtgag aagagctgcc
     2341 gagctgctgt ctagaaccac agccgtggct ctgagaacat acttccccga cgacgacaac
     2401 gctaagatcc tggccaactt catcgagaag gtggacgtgt ggttcagcat cagcaacagc
     2461 tacaccccct tcgccaagct ggacttcaag aaggcctaca ccgccagcga tgatcaggtt
     2521 agagccctga ccgacatgta cgacatcatc agcaacatga ccatccccgg caagaatggc
     2581 ctgcagatct tccagagaag catcatgatg cagatcaaga gcctgcagat ggtgttcgcc
     2641 gacatgaagc tgaagcacga cgccaagttc atctgcaccc acaagctgaa ccaggacgtg
     2701 ctggagaact tcttcagcca gctgagacaa atcggcggcg tgtacgatca tcctagcccc
     2761 ctgagctgca tctacagaat cagactgatg atcctgggca agacccccac cattctgcac
     2821 aaccagacca caacagtgga ggccgctaat tgtgagcagg acgagttcat caccaccacc
     2881 gagagcaatg ttgccggcgc tagaagcaat gacgtgttca tcagcgccag catgttcgag
     2941 aaggccgaga tcacacccga actgcctgac gtgaaggcta tggaggaggc caacaacttc
     3001 gtgagccagg acgtgagctc tcttagcagc gtgagcgaca caagcatcga gctgcctcac
     3061 caggaagctg atggcctgag ctatatcctg ggctacctgg ccaagaaaca ccacagccag
     3121 ttcagccacc tgaatctggg cgagcacacc ttcaagacca gaatcgacca caactactgc
     3181 cagcctccca cctttgtgca tcacctgagc tgtggcggcc tgattgaacc tagcgacgag
     3241 ttcctgaacc tgggcaagaa gatggagaag atcttcctga agatgaaccc cgatggcgga
     3301 cttctgaaag gcgagagaat cgtggacaga atcaccaaca agatcaagag acacctgacc
     3361 gaactgcccg tggagatcat cagaagcttc gccaagcaga gagtgatcgt gagaatgaga
     3421 tacctgaacc tgaaggccac agctgagcag ctgaacaaga tgaagcacaa gaagagaaag
     3481 ttcgtgaccg agaacaccaa ggccgccaag aagatgaaga agatcatcaa cgcggccgct
     3541 cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     3601 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3661 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3721 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3781 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     3841 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     3901 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3961 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4021 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4081 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4141 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4201 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4261 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     4321 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4381 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4561 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     4621 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gaaatgaccg accaagcgac
     4681 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4741 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4801 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4861 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4921 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4981 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5041 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5161 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5221 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5281 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5341 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5401 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5461 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5521 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5581 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5641 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5701 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5761 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5821 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5881 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5941 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6001 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6061 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6241 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6301 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7021 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7081 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7141 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     7201 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     7261 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     7321 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
