LOCUS       Exported                6508 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="CMV enhancer;human cytomegalovirus immediate early 
                     enhancer;color: #ffffff"
     promoter        381..584
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="CMV promoter;human cytomegalovirus (CMV) immediate 
                     early promoter;color: #ffffff; direction: RIGHT"
     promoter        629..647
                     /locus_tag="T7 promoter"
                     /label=T7 promoter
                     /note="T7 promoter;promoter for bacteriophage T7 RNA 
                     polymerase;color: #ffffff; direction: RIGHT"
     promoter        757..775
                     /locus_tag="T7 promoter(1)"
                     /label=T7 promoter(1) T7 promoter
                     /label=T7 promoter(1)
                     /note="T7 promoter;promoter for bacteriophage T7 RNA 
                     polymerase;color: #ffffff; direction: RIGHT"
     CDS             847..2658
                     /codon_start=1
                     /label=26- Mariner-1_PM
                     /translation="MSMKRTARKKAPAVAPKRQRKMLTIAEKVGLLDMLKEGRSYAAVG
                     RHYGINESSVRYIKKEENNIRTTAAISFNRDAKRVVTVRNKTIVRMESALALWISDSRK
                     KNIPLDTNIIRTKAKKLYETFADSEEQDDAEPGPSKSAFHAEPSPFNASKGWFDKFQQR
                     FGLKSVSLHGEAASANTAGAEAYVNKCKVIIEEGGYKPEQVFNMDETGLFWKRMPSRTF
                     IMQDEAKASGFKAQKDRVTLVMCGNAAGFMIKPGLIYRSKNPRALKNKNKNALPVYWMH
                     NAKAWMTKVLNLDWFQHCFIPEVKRYLRGKGLDFKVLLLVDNAGGHADDLSYDGVQIEF
                     LPPNTTSLIQPLDQGIIRAFKALYTRNTLQHLVEAMDSDQDFSLKAYWREYTIASCLQN
                     IQRAIHEMKTETLNVCWKKKLWPEAVHNPTGCSLDEIHHSAVDTAVNLAKQLGGDGFND
                     MTPDDINALIDAHSQPLTDEDLAEMTKPPSEDDGEEEEEDTSVDKEEEDGLTLDRLATM
                     VRMATELQRAAQEWDPLMFRSLQFSNIIEGGMSVYKNLLAQKKKQRQQLPITMFCTRKK
                     TPAPRPSEEKDVTAERRQDKEAPSEEL"
     promoter        complement(2696..2714)
                     /locus_tag="SP6 promoter"
                     /label=SP6 promoter
                     /note="SP6 promoter;promoter for bacteriophage SP6 RNA 
                     polymerase;color: #ffffff; direction: LEFT"
     polyA_signal    2740..2964
                     /locus_tag="bGH poly(A) signal"
                     /label=bGH poly(A) signal
                     /note="bGH poly(A) signal;bovine growth hormone 
                     polyadenylation signal;color: #a6acb3"
     rep_origin      3010..3438
                     /direction=RIGHT
                     /locus_tag="f1 ori"
                     /label=f1 ori
                     /note="f1 ori;f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis;color: #ffff00"
     promoter        3452..3781
                     /locus_tag="SV40 promoter"
                     /label=SV40 promoter
                     /note="SV40 promoter;SV40 enhancer and early 
                     promoter;color: #ffffff; direction: RIGHT"
     rep_origin      3632..3767
                     /locus_tag="SV40 ori"
                     /label=SV40 ori
                     /note="SV40 ori;SV40 origin of replication;color: #ffff00"
     polyA_signal    3947..4068
                     /locus_tag="SV40 poly(A) signal"
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal;SV40 polyadenylation 
                     signal;color: #a6acb3"
     primer_bind     complement(4117..4133)
                     /locus_tag="M13 rev"
                     /label=M13 rev
                     /note="M13 rev;common sequencing primer, one of multiple 
                     similar variants;color: #a020f0; direction: LEFT"
     protein_bind    4141..4157
                     /locus_tag="lac operator"
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator;The lac repressor binds to the lac 
                     operator to inhibit transcription in E. coli. This 
                     inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG).;color: 
                     #31849b"
     promoter        complement(4165..4195)
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="lac promoter;promoter for the E. coli lac 
                     operon;This reverse directional feature has 3 segments: 
                     ???1:?4138?..?4144?/?#ffffff?/?-10 
                     ???2:?4145?..?4162?/?#ffffff 
                     ???3:?4163?..?4168?/?#ffffff?/?-35"
     rep_origin      complement(4519..5107)
                     /direction=LEFT
                     /locus_tag="ori"
                     /label=ori
                     /note="ori;high-copy-number colE1/pMB1/pBR322/pUC origin of
                     replication;color: #ffff00"
     CDS             complement(5278..6138)
                     /codon_start=1
                     /gene="bla"
                     /locus_tag="AmpR"
                     /product="beta-lactamase"
                     /label=bla
                     /label=AmpR
                     /note="AmpR;confers resistance to ampicillin, 
                     carbenicillin, and related antibiotics;This feature has 2 
                     segments: ???1:?5251?..?6042?/?#ccffcc 
                     ???2:?6043?..?6111?/?#ccffcc?/?signal?sequence Cleavage 
                     site after base 6042"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6139..6243)
                     /gene="bla"
                     /locus_tag="AmpR Promoter"
                     /label=bla AmpR promoter
                     /label=AmpR Promoter
                     /note="AmpR promoter;color: #ffffff; direction: LEFT"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcatgaaacg caccgcacgc aagaaagcac ctgcggttgc gcctaagcgt
      901 caacgtaaga tgctgaccat tgccgaaaaa gtgggcctgc tggatatgct gaaagaaggc
      961 cgtagctacg cggcagttgg tcgccattat ggcatcaacg aaagcagcgt gcgctacatc
     1021 aaaaaggagg agaacaacat tcgtaccacc gcggcgatta gctttaaccg cgacgcgaaa
     1081 cgtgtggtga ccgttcgcaa caagaccatt gtgcgcatgg agagcgcatt agcgctgtgg
     1141 ataagcgata gccgcaaaaa gaacattccg ctggacacca acattattcg caccaaagcg
     1201 aagaagctgt atgaaacctt tgcggacagc gaagaacagg atgatgcgga acctggccct
     1261 agcaaatcag cgtttcacgc ggaaccgagc ccttttaacg caagcaaagg ctggttcgac
     1321 aaatttcagc agcgcttcgg cttaaagagc gtgagcctgc atggtgaagc agcgagcgcg
     1381 aatactgcag gtgcggaagc gtatgtgaac aagtgcaagg tgatcattga ggagggcggc
     1441 tacaaaccgg aacaggtgtt taacatggat gaaaccggcc tgttttggaa acgtatgccg
     1501 agccgcacct ttatcatgca ggatgaagcg aaggcgagcg gttttaaggc ccagaaagat
     1561 cgcgtgaccc tggttatgtg tggtaatgcc gcgggcttta tgattaagcc tggcctgatc
     1621 tatcgcagca agaatccgcg cgcgctgaaa aacaaaaaca agaacgccct gcctgtgtac
     1681 tggatgcaca acgccaaagc ctggatgacc aaagtgctga acctggattg gttccagcat
     1741 tgcttcattc cggaggtgaa gcgttacctg cgcggtaaag gcctggactt caaagtgctg
     1801 ctgctggtgg ataatgcagg tggccatgcc gatgatctga gctatgacgg cgtgcagatt
     1861 gaatttctgc ctccgaacac caccagctta atccagcctc tggaccaggg tattattcgc
     1921 gcgtttaaag cgctgtatac ccgcaacacc ctgcagcatc tggtggaagc gatggatagc
     1981 gatcaggatt tcagcctgaa agcctattgg cgcgagtaca ccatagcgag ctgcctgcag
     2041 aatatccagc gcgccattca tgaaatgaag accgaaaccc tgaacgtgtg ctggaaaaag
     2101 aaactgtggc cggaagcggt gcataatcct actggctgca gcctggatga aattcaccac
     2161 agcgcggttg ataccgcggt gaatctggcc aaacagctgg gtggcgatgg ctttaacgac
     2221 atgacccctg acgatatcaa cgccctgatc gatgcacata gccagccgct gaccgatgaa
     2281 gatctggccg aaatgaccaa accgccgagc gaagatgatg gcgaagaaga ggaagaggac
     2341 accagcgtgg ataaagagga ggaggatggc ttaaccctgg atcgcctggc aaccatggtt
     2401 cgtatggcga ccgaattaca gcgtgccgcc caagaatggg atcctctgat gtttcgcagc
     2461 ctgcagttca gcaacattat tgaaggcggc atgagcgtgt ataagaacct gctggcgcag
     2521 aaaaaaaaac agcgccagca gctgccgatt accatgttct gcacccgcaa aaagacccct
     2581 gcacctcgtc ctagcgaaga aaaagacgtg accgcggaac gccgtcaaga taaggaagcc
     2641 ccgagcgaag aactgtaatg agcggccgct cgagcatgca tctagagggc cctattctat
     2701 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2761 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2821 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2881 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2941 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3001 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3061 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3361 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3421 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3481 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3541 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3601 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3721 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3781 aaagctcccc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3841 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3901 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     3961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     4081 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5401 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5581 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5761 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6241 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     6301 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6361 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6421 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6481 cgcgatgtac gggccagata tacgcgtt
//
