LOCUS       Exported                7305 bp ds-DNA     circular SYN 26-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   fdfasfdaffdagdga
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jun 27, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3510..3511)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3537..3761
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3807..4235
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4249..4578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4429..4564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4744..4865
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4914..4930)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4938..4954
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4962..4992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5007..5028
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5316..5904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6075..6935)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6936..7040)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgc ctaacaacag ctgcgccgtg agcttttgta agaacagcac ctaccgggcc
      901 aagacccaga acctgaacat ctccttccac cggtttccta agcatgagga tctgagaaac
      961 cagtggacac tgttctgcca gagggaggag gtgtggaagc ccaaggataa cgatgtgatt
     1021 tgcagctgcc actttacaat cgagaactac cagatggcta agagcccaat cctcaagaac
     1081 cggcagcccc tgaggatcct gacccccaac gccattccca gcctggtctg caggaacccc
     1141 cacaccattc ctgagcagga gcagattgat agagtggatg agcaccagag gattaacgag
     1201 ctgtacgaga ggaacgacag caagagcgct tccgacttcg tggaccacga ggccagcgtc
     1261 ctgatcagcg atcaggagaa gatccagaac ctcgaggccg tgatcgccac actgacagag
     1321 gagattgact tttgccggaa gctgaggaag gataacagct ttctgctgaa gcagaagaag
     1381 attctgctgc agcagaacaa gaagctgaag ctcaggaaca cagtcctgaa gaaccggatt
     1441 agggtgctcg agcagacaac catccagcat cacgagatcg tgcccctcat gaagaagaag
     1501 ctgagtaaga cactgagcgg gaaccagatc gatctcatcc tcaagaggaa gaagagggtg
     1561 cggtggacaa agaaggagct gggggccgcc ctgactctgc ggtacttcgg caagcggccc
     1621 tacatgtaca tgactgatga tatgcacttc cctctgcccg ccagagccac aatccagcgg
     1681 tacattaaga gcattaacat caagcagggc atcctggagg acgtcctgaa cctgatggag
     1741 tcctacgcca agacactgat ccccagggat agggagtgcg tgatctcctt tgacgagatg
     1801 aaggtcaccc acatcctgga gtacgacgtc ggggccgatg aggtcctggg cccccacaac
     1861 tacctgcagg tggtcatggc caggggcatc tttaacaagt ggaagcagcc catcttcatc
     1921 ggcttcgacc agaagatgac caaggatatt atcctgagcc tgattaagaa gctgagcgag
     1981 aggatgatta acgtggtggc catcgtgagc gataactgcc agactaacat cagctgctgg
     2041 cgggatctgg gcgcccacga cattaccaag ccctacttta accaccctat caccaacaag
     2101 aacgtgtacg tctttcctga cgccccccat ctgatcaagc tcctgcggaa ctggttgatt
     2161 gacacagggt ttgattacaa ggggagcatc atcaccgccg acccactcag aaagctggtg
     2221 gaggagagaa aggatgccga gatcacccca ctcttcaagc tgaacgagaa ccacttgacc
     2281 atgagcccac aggagcggca gaacgtcagg cgggccgtcc agctgctgtc ccacaccacc
     2341 gctacagccc tgcggaggta ccagagcgac gatgccagcc agactctggc cgagtttatt
     2401 gagaccgtcg attgctggtt tagcatctcc aactcctaca gcccttgggc caagattgcc
     2461 tacaagcggt cctacgccgg gaaggaggag caggagaagt ccctggacaa gatgtacgag
     2521 ctgatttcca acatgaccgc cctgaacagg actagcatgc agaccttcca gaagtccatc
     2581 ctcatgcaca ttacatccct gaagatgctg tacaaggaca tgcagaagaa gcaccaggtg
     2641 gactttatca gcacacacaa gctgaaccag gacattctgg agaacttctt cagccagctg
     2701 cgccagaagg ggggggtcta cgaccaccca tcccccctga gctgcctgca ccggatccgg
     2761 atgatcgtga tcgggaagtc ccccacaatc ctgattaacc agactaacct ggagacaaac
     2821 cggaacaccc ggctgggaga gaacatctgc ctgatggagc agcacgagaa cgccaacgag
     2881 gactgcctgt ccgagcagaa ccaggacttc atgagcgtga ccctgtttac cgaggccgat
     2941 gtgattcccg atctgcccga caacgccctg aacgcccacg aaatggatga tgagagcgag
     3001 atcctcagca ccatcagcag cacaatccct gacttgcccg agcaggacag ggatggcttc
     3061 gagtacatta tgggctacct gggcaagaag tttcacaaga agcaccctca catcgaccag
     3121 gggacatact ccttcaagat tacctcagat cacaactact ccaagccacc cagctttgtg
     3181 aagcacctga gcacaggcgg gttgttcgtg cccagccaca gcttcctgaa gctcggcagc
     3241 aagatggaga agattttcca gaagctgcac cccgatggca ctctggataa gaagccagga
     3301 atcgtcaaga caggcgtgaa cacaattaag aagaagatcc ccagcctgcc cgtggatatg
     3361 attaagtgtt tcatgatgct gagagtgaag ctgaggatta agcacaccaa cctgaaggcc
     3421 gctaacgaga agctgctgag atgcaagcgg aagggcccca aggagcacaa ggccgccaag
     3481 aagatgaaga agattgtgaa cgcggccgca tgctagagct cgctgatcag cctcgactgt
     3541 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3601 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3661 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3721 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3781 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4201 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     4261 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4321 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4381 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4441 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4501 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4561 ggcctaggct tttgcaaaaa gctccccgaa atgaccgacc aagcgacgcc caacctgcca
     4621 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4681 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4741 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     4801 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     4861 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     4921 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4981 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5041 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5101 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5161 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5221 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5281 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5341 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5401 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5461 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5521 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5581 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5641 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5701 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5761 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5821 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5881 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5941 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6001 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6061 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6181 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     7081 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7141 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     7201 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     7261 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
