LOCUS       Exported                5773 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             832..1934
                     /codon_start=1
                     /label=Tc1-5_Xt
                     /translation="EFAATMIGYKKSFSEWQCLSEAKMGRGSPIPTMLRRKIVEQYQKG
                     VTQRKIAKILHLSSSTVHNIIRRFRESGTISVRKGQGRKTILDARDLRALKRHCTTNRN
                     ATVKEITEWAQEYFQKPLSVNTIHRAIRRCQLKLYSAKKKPFLSKIHKLRRFHWARDHL
                     KWSVAKWKTVLWSDESRFEVLFGNLGRHVIRTKEDKDNPSCYQRSVQKPASLMVWGCMS
                     ACGMGSLHVWKGSINAEKYIQVLEQHMLPSRRHLFQGRPCIFQQDNARPHSASITTSWL
                     RRRRIRVLKWPVCSPDLSPIENIWRIIKRKVRQRRPKTIEQLEACIRQEWESIPIPKLE
                     KLVSSVPRRLLSVVRRRGDATQW**AA"
     promoter        complement(1961..1979)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2005..2229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2275..2703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2717..3046
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2897..3032
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3212..3333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3382..3398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3406..3422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3430..3460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3475..3496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3784..4372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4543..5403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5404..5508)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga tcggctacaa aaaaagcttt agcgagtggc aatgcctgag cgaagcgaaa
      901 atgggtcgcg gcagccctat tcctactatg ctgcgccgca aaattgtgga gcagtaccag
      961 aagggtgtga cccagcgcaa aattgcgaag atcctgcact taagcagcag caccgtgcac
     1021 aacattatcc gccgctttcg cgaaagcggc accatcagcg tgcgtaaagg ccaaggccgc
     1081 aaaaccattt tagacgcgcg cgatttacgt gcgctgaagc gtcattgcac caccaatcgc
     1141 aacgcgaccg tgaaagagat caccgaatgg gcgcaggaat atttccagaa gccgctgagc
     1201 gtgaacacca ttcatcgcgc gatccgtcgc tgtcagctga aactgtatag cgccaagaaa
     1261 aagccgtttc tgagcaaaat ccataaactg cgccgctttc attgggcacg cgatcatctg
     1321 aaatggagcg tggcgaaatg gaaaaccgtg ctgtggagcg atgagagccg ctttgaagtg
     1381 ctgttcggca acttaggccg ccatgtgatt cgcaccaagg aagataagga taacccgagc
     1441 tgctatcagc gcagcgtgca aaaacctgcg agcctgatgg tttggggttg catgagcgcg
     1501 tgtggcatgg gtagcttaca tgtgtggaag ggcagcatta acgcggaaaa gtatattcag
     1561 gtgctggagc agcatatgtt acctagccgc cgccatttat ttcaaggccg cccgtgcatt
     1621 tttcagcagg acaacgcccg ccctcatagc gcatcaatca ccaccagctg gttacgtcgt
     1681 cgtcgtattc gtgtgctgaa atggcctgtg tgcagccctg atctgagccc tattgaaaac
     1741 atctggcgca tcattaaacg caaagtgcgc cagcgtcgcc ctaaaaccat cgaacagctg
     1801 gaagcgtgca tacgccagga atgggaaagc attccgattc cgaaactgga gaaactggtg
     1861 agcagcgtgc ctcgtcgttt actgagcgtg gttcgtcgtc gtggtgatgc cacccaatgg
     1921 tgatgagcgg ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg
     1981 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     2041 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     2101 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     2161 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     2221 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     2281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     2341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     2401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     2461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     2521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     2581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2701 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     2761 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     2821 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     2881 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     2941 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     3001 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgaaat
     3061 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3121 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3181 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     3241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3301 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     3361 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     3421 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     3481 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     3541 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     3601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     4321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     4381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     4561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4681 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5461 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5521 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     5581 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     5641 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     5701 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     5761 agatatacgc gtt
//
