LOCUS       Exported                6052 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2202
                     /codon_start=1
                     /label=Mariner-1_XT
                     /translation="MDTGTTNPGPKQKRRSYEAGFKLKVVSRAEESNNSIASREFCVDE
                     KQVREWRKMKAGLEKIPKTKKARRGLMTSYGALETELHEWVMACRQNGYCVTRMGIRLR
                     ALQMAKDDKYKAPGIEKFAASAGWCTRFMNRFDLCLRQRTKISQKLPRDLEEKVMSFQS
                     FIIKQRRIHNYDLGDIGNMDETPMTFDLPSNRTVASLGDKTIFLRTTGNEKNHFTVVLS
                     CLANGTKLRPVILFKRKTLPKKVKFPPRITVRPHVKGWMDEDGTKKWLEEIWNGRPGAA
                     LKKKPSLLVWDMFRAHTSDDIKELAKSSQVTLAVIPGGLTSVLQPLDVSLNKPFKDRVR
                     KKWHEWMSSGQARLTKVGNLMKPDIELIAKWVRDAWEDIPEDMVQRAFKKCGISNAMDG
                     SEDSALYENDSSDGDDCELSDDNVYADNLTPAEAEALFGHTDDEEESSFEGF"
     promoter        complement(2240..2258)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2284..2508
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2554..2982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2996..3325
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3176..3311
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3491..3612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3661..3677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3685..3701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3709..3739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3754..3775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4063..4651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4822..5682)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5683..5787)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg ataccggcac caccaatcct ggccctaaac agaaacgccg cagctatgaa
      901 gcaggcttca agctgaaggt tgtgagccgt gcggaagaga gcaacaacag cattgccagc
      961 cgcgaatttt gcgtggatga aaaacaggtg cgcgagtggc gcaaaatgaa agccggcctg
     1021 gagaagattc cgaaaaccaa aaaggcgcgc cgcggcttaa tgacctcata tggcgcgctg
     1081 gaaaccgagt tacacgaatg ggtgatggcc tgtcgtcaga acggctactg tgttacccgc
     1141 atgggcattc gtctgcgtgc gttacagatg gcgaaggacg acaagtataa agccccgggc
     1201 attgaaaaat ttgccgccag cgcaggttgg tgtacccgtt ttatgaaccg ctttgatctg
     1261 tgcttacgcc agcgcaccaa aatcagccag aaactgcctc gcgatctgga ggaaaaggtg
     1321 atgagcttcc agagcttcat tattaaacag cgccgcatcc acaactatga tctgggcgac
     1381 atcggcaaca tggacgaaac cccgatgacc ttcgatctgc cgagcaatcg taccgttgcg
     1441 agcttaggcg ataaaaccat tttcctgcgc accaccggca atgagaagaa ccactttacc
     1501 gtggtgctga gctgcttagc aaacggtacc aaactgcgcc cggtgattct gtttaagcgc
     1561 aaaaccctgc cgaaaaaagt gaaatttccg ccgcgcatca ctgttcgccc tcatgtgaaa
     1621 ggctggatgg acgaggatgg caccaaaaaa tggctggaag aaatctggaa cggccgtcct
     1681 ggtgcagccc tgaagaaaaa accgagcctg ctggtgtggg atatgtttcg cgcgcatacc
     1741 agcgatgata ttaaagaact ggccaaaagc agccaggtga ccttagccgt tattccgggt
     1801 ggtctgacct cagttctgca acctctggac gtgagcctga acaaaccgtt taaagatcgc
     1861 gtgcgcaaaa agtggcacga gtggatgagc tcaggtcaag cgcgtctgac caaagtgggc
     1921 aacctgatga aacctgacat tgagctgatt gcgaaatggg tgcgcgatgc gtgggaagat
     1981 attcctgaag acatggtgca gcgcgcgttt aaaaagtgcg gcattagcaa cgcgatggat
     2041 ggcagcgaag atagcgcgct gtatgaaaac gacagcagcg atggcgacga ttgcgaactg
     2101 agcgacgaca acgtgtacgc ggataatctg acccctgcgg aagcggaagc gttatttggc
     2161 cacaccgatg atgaagaaga gagcagcttt gaaggctttt gatgagcggc cgctcgagca
     2221 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     2281 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2341 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2401 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2461 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     2521 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     2581 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2641 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2701 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2761 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2821 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2881 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2941 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3001 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3061 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3121 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3181 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3241 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     3301 ttttggaggc ctaggctttt gcaaaaagct ccccgaaatg accgaccaag cgacgcccaa
     3361 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     3421 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     3481 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3541 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3601 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     3661 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3721 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3781 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     3841 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5701 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5761 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     5821 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     5881 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     5941 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     6001 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
