LOCUS       Exported                5791 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..1941
                     /codon_start=1
                     /label=hAT-1B_PM
                     /translation="MVSAVCSDGAPAMLGRHSGFGALVKSDAPHIIVTHCVLHRHALAT
                     KTLPPKLAEVLKIVVECVNYVRNSAMKHRIFKELCKEMGSEFEVLLYHSNVRWLSRGKV
                     LNRVFAMRVELALFLREYQHCHADCFENSEFILILAYMADIFDALNHLNRQMQGGGVNI
                     IEAEENLKAFKKKLPLWKRRTENDNFANFPLLDDCVSKIEDVSGIGDISVPGELKQAIA
                     MHLDELAKSLDGYFPTRESYPAWVRQPFTFSVATADVNDEYLEEIIELQQSQLQQQLFR
                     TTTLSTFWCHQIVPYPLIAKKALEILTPFVTTYLCEQSFSRMVDIKTKKRNRLCCENDM
                     RVALAKVKPRISELVSERQQQKSH"
     promoter        complement(1979..1997)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2023..2247
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2293..2721
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2735..3064
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2915..3050
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3230..3351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3400..3416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3424..3440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3448..3478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3493..3514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3802..4390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4561..5421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5422..5526)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg tgagcgcggt ttgttcagat ggtgcaccgg cgatgttagg tcgtcatagc
      901 ggttttggcg cgctggttaa atcagacgcc ccgcatatta tcgtgaccca ttgcgtgtta
      961 catcgtcatg cgctggcgac caaaactctg cctccgaagt tagcggaggt gctgaaaatt
     1021 gtggtggaat gcgtgaacta tgtgcgcaac agcgcgatga aacatcgcat ctttaaggag
     1081 ctgtgcaaag aaatgggcag cgagtttgag gtgctgctgt accatagcaa cgtgcgttgg
     1141 ctgagccgtg gcaaagtgct gaatcgcgtg tttgccatgc gtgttgaact ggccctgttt
     1201 ctgcgcgaat atcagcattg ccacgccgat tgcttcgaaa acagcgagtt cattctgatt
     1261 ctggcctaca tggccgatat cttcgatgcc ctgaaccacc tgaaccgcca aatgcaaggt
     1321 ggcggcgtga acattattga agcggaggaa aacctgaagg ccttcaaaaa aaagctgcct
     1381 ctgtggaaac gccgcaccga aaacgataac tttgcgaact ttcctctgct ggatgactgc
     1441 gtgagcaaaa ttgaagatgt gagcggcatt ggcgatattt cagtgccggg cgagttaaaa
     1501 caagccatcg cgatgcactt agatgaactg gccaaaagcc tggatggcta ctttcctacc
     1561 cgcgagagct atccggcgtg ggttcgtcag cctttcacct tttcagtggc gaccgccgat
     1621 gtgaacgatg agtacctgga ggagattatt gaactgcagc agagccagtt acagcagcag
     1681 ctgtttcgta ccaccaccct gagcaccttt tggtgccatc agattgtgcc gtatccgctg
     1741 atcgcgaaaa aagccctgga aattctgacc ccgttcgtga ccacctatct gtgcgagcag
     1801 agctttagcc gcatggtgga catcaaaacc aaaaagcgca accgcctgtg ctgcgagaac
     1861 gatatgcgtg ttgcgctggc gaaagtgaaa cctcgcatta gcgagctggt tagcgaacgc
     1921 cagcagcaga agagccattg atgagcggcc gctcgagcat gcatctagag ggccctattc
     1981 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     2041 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2101 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2161 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2221 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     2281 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2341 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2401 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2461 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2521 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2581 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2641 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2701 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2761 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2881 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2941 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3001 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3061 caaaaagctc cccgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     3121 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     3181 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     3241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3361 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3421 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3481 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     3721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     3781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     3841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     3901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5521 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     5581 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     5641 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     5701 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     5761 cttcgcgatg tacgggccag atatacgcgt t
//
