LOCUS       Exported                6628 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    832..2789
                     /label=Myotis_hAT1
     promoter        complement(2816..2834)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2860..3084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3130..3558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3572..3901
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3752..3887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4067..4188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4237..4253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4261..4277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4285..4315)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4330..4351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4639..5227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5398..6258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6259..6363)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg agaaccagaa gaacaaaaaa gcgcgcctga acgataaagg tagcggcagc
      901 tcatcatcac gtccgttcca ggatatctgg accgaaatgt acggcatcgt ggaccgtaac
      961 aaccgcagct tatgcatcct gtgcaacgaa accgttgtga gccgcacctg gaatgtgaaa
     1021 cgccactttg aaaccaacca ttgccagctg ctgaagaaaa gcatcgatga gcgcaaggaa
     1081 tacattagcc gccagctgca cctgtataaa agccagagca acagcattat taagtttgtg
     1141 cgctgcagca ccaatttaac cagcgcgagc tattgcattg cgcacagcat tgcccagcat
     1201 ggcaaagcgt taagcgatgg cgagtttatt aaggaaacct ttctgcgctg tgcaccggcg
     1261 ctgtttcatg atatgaagaa caaggacgac atcatcaaac gcattagcga gttaccgctg
     1321 agccgcaaca ccattaaaga ccgcattatc gacctgaaca agaacgtgca gcatcagctg
     1381 aaaaaggatc tgaacagctg caagtatttt agcatcagcc tggatgaaac caccgatgtg
     1441 accagcaatg cgcgtctggc gataatagcg cgctatagcg atggtctgac catgcgcgaa
     1501 gaactgatta aactggaaag cgtgccgatt agcactagcg gcaacgagat ttgcaaggtg
     1561 gtgattaaga cctttagcga cctgaacatc gatattagca agattgtgag cgtgaccact
     1621 gatggcgcgc ctaatatggt gggtaagaac gtgggctttc tgaaactgtt catggaagcg
     1681 attcgccatc ctctggtgcc gtttcattgc attattcacc aggaagtgct gtgtgcgaaa
     1741 agcggcttta gcgaactgaa cgacctgatg agcgtggtga ccaaaatcgt gaactttatt
     1801 gccagccgcc cgctgcataa acgcgaattt agcgcgctgc tgcaggaagt ggatagcacc
     1861 tatagcggcc tgctgatgtt taacaacgtg cgctggctga gccgtggtaa agtgctggaa
     1921 cgctttgtgg aatgctttga agagatcacc gtgttcattg agaacaaaga cctggcgaac
     1981 tttcctcaga ttaacgatca taagtgggtg agcaacctga tgttcttcac cgatctgagc
     2041 gtgcatatga acgaactgaa cctgaaactg cagggcttcg gcaagagcat tgacgtgatg
     2101 tttggctaca tcaagagctt tgagagcaag ctgaaaatct ttaagcgcga catcgagact
     2161 aaaacctata agtatttccc gcgcatcaag aagtatttcg aaaaagcgac caccaccagc
     2221 gtgatggaaa gcattctgat gtaccagaac attgtgaaca gcctgtttga gcagttcagc
     2281 gagcgctttg accagtttcg cagcctggaa cagaccatca aaatcatcaa gtaccctgat
     2341 gtggtggtgt atagcaccct ggaactgaaa gattttcagt ggatgcagat tgatgacctg
     2401 gaaatgcagc tggccgattt ccagggcagc atttggaccc atgtgtttgt ggatctgcgc
     2461 agcaaactgg aaaacctgga gcgcaaccgc ctgaacaacg aagaggagtg caactatgaa
     2521 caggagattc tgagcgcgtg gaaccgtgtg cctgatacct ttagcgacat taaaaacctg
     2581 gccatggcgc tgctgaccat ttttagcagc acctatttct gcgaaaccct gtttagcgcc
     2641 ctgaacaaca ttaaaaccaa caaacgcaac cgcctgaccg atgaagtgag cggtgcatgt
     2701 ctggcgctga aatgcaccaa atatgagccg gcgattgaag aactggccga taaactgcag
     2761 caccagaaaa gccattgatg agcggccgct cgagcatgca tctagagggc cctattctat
     2821 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2881 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2941 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3001 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3061 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3121 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3541 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3901 aaagctcccc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3961 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     4021 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     4081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     4201 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4441 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5041 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6361 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     6421 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6481 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6541 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6601 cgcgatgtac gggccagata tacgcgtt
//
