LOCUS       Exported                5722 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 07-11-2019 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        524..585
                     /label=cytomegalovirus_minimal_promoter
                     /note="cytomegalovirus_minimal_promoter"
     primer_bind     629..648
                     /label=T7
                     /note="T7"
     promoter        629..647
                     /label=T7_promoter
                     /note="T7_promoter"
     primer_bind     757..776
                     /label=T7
                     /note="T7"
     promoter        757..775
                     /label=T7_promoter
                     /note="T7_promoter"
     gene            832..1883
                     /label=TC1_FR2
                     /note="59-TC1_FR2"
     promoter        complement(1912..1929)
                     /label=Sp6_promoter
                     /note="Sp6_promoter"
     rep_origin      2241..2547
                     /label=f1_replication_origin
                     /note="f1_replication_origin"
     gene            complement(2657..2892)
                     /label=SV40_enhancer_region
                     /note="SV40_enhancer_region"
     promoter        2679..2947
                     /label=SV40_promoter2
                     /note="SV40_promoter2"
     promoter        2799..3000
                     /label=SV40_promoter
                     /note="SV40_promoter"
     rep_origin      2846..2923
                     /label=SV40_origin_of_replication
                     /note="SV40_origin_of_replication"
     primer_bind     complement(3330..3347)
                     /label=M13R
                     /note="M13R"
     promoter        complement(3338..3378)
                     /label=trc_promoter
                     /note="trc_promoter"
     primer_bind     complement(3343..3366)
                     /label=M13R-48
                     /note="M13R-48"
     primer_bind     complement(3344..3367)
                     /label=RV_M
                     /note="RV_M"
     gene            complement(3353..3375)
                     /label=lac_operator
                     /note="lac_operator"
     promoter        complement(3380..3409)
                     /label=lac_promoter
                     /note="lac_promoter"
     primer_bind     complement(3576..3592)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(3718..4337)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(4492..5352)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(5394..5422)
                     /label=Amp promoter
                     /note="Amp promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg tgcgctatct ggatgccacc gaagttgcac aggtggtgca gttactgcaa
      901 gacggcacca gcatacgcgc agttgcacgt cgttttgcgg ttagccctag caccgtttca
      961 cgtgcgtggc gccgttttca agaaaccggc agctatagcc gtcgtgcagg tcagggtcgt
     1021 cgtcgtagcc tgaaccctca acaggatcgc tacctgctgt tatgtgcgcg tcgtaatcgc
     1081 atgtcaaccg cgcgtgcatt acagaacgat ctgcaacagg ccaccggtgt taacgttagc
     1141 gaccagacca tccgcaatcg tctgcatgaa ggtggtttac gcgcgcgtcg tcctgttgtg
     1201 ggtcctgttt taaccgcgcg tcatcgccgt gcacgcttag catttgcgat cgatcatcag
     1261 aactggcagt tacgtcattg gcgcccggtg ttatttaccg acgagagccg ctttaacctg
     1321 agcacctgcg atcgtcgtga acgtgtgtgg cgttgtcgcg gtgaacgtta tgcggcgtgc
     1381 aacattattc agcacgaccg ctttggtggc ggtagcgtta tggtttgggg cggcattagc
     1441 ttagaaggtc gcaccgatct gtaccgtctg gataacggca ccttaaccgc catacgctat
     1501 cgcgatgaaa ttctgggccc tatcgtgcgt acttatgccg gtgcggttgg ccctggcttt
     1561 ttactggtgc acgacaatgc acgtccgcat gttgcacgtg tttgccgtca gtttctggag
     1621 gatgaaggca ttgataccat tgactggcct cctcgctcac ctgatctgaa ccctatcgaa
     1681 catctgtggg atatcatgtt ccgcagcatc cgtcgccgtc aagtggatcc tcaaaccgtg
     1741 caggaactga gcgatgcgtt agtgcagatc tgggaggaaa tcccgcagga taccattcgc
     1801 cgcctgattc gttcaatgcc tcgccgttgt caggcgtgtg ttcaagcacg tggcggtcac
     1861 accaactact aatgagcggc cgctcgagca tgcatctaga gggccctatt ctatagtgtc
     1921 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     1981 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     2041 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     2101 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     2161 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     2221 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2641 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     2701 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     2761 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     2821 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     2881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     2941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3001 ccccgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3061 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3121 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     3181 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3241 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     3301 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3361 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3421 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3481 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3541 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3721 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3781 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3841 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3901 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3961 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4021 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4081 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4141 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4201 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4261 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4321 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4381 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4441 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4501 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4561 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4621 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4681 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4741 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4801 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4861 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4921 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4981 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5041 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5101 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5161 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5221 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5281 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5341 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5401 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5461 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     5521 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     5581 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     5641 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     5701 gtacgggcca gatatacgcg tt
//
