LOCUS       Exported                6148 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Qinglan Biotech
  TITLE     Synthetic gene from Qinglan Biotech
  JOURNAL   Exported 19-03-2020 from Plasmap http://www.qinglanbiotech.com
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        524..585
                     /label=cytomegalovirus_minimal_promoter
                     /note="cytomegalovirus_minimal_promoter"
     primer_bind     629..648
                     /label=T7
                     /note="T7"
     promoter        629..647
                     /label=T7_promoter
                     /note="T7_promoter"
     primer_bind     757..776
                     /label=T7
                     /note="T7"
     promoter        757..775
                     /label=T7_promoter
                     /note="T7_promoter"
     gene            832..2309
                     /label=hAT-3_Gav
                     /note="71,hAT-3_Gav"
     promoter        complement(2338..2355)
                     /label=Sp6_promoter
                     /note="Sp6_promoter"
     rep_origin      2667..2973
                     /label=f1_replication_origin
                     /note="f1_replication_origin"
     gene            complement(3083..3318)
                     /label=SV40_enhancer_region
                     /note="SV40_enhancer_region"
     promoter        3105..3373
                     /label=SV40_promoter2
                     /note="SV40_promoter2"
     promoter        3225..3426
                     /label=SV40_promoter
                     /note="SV40_promoter"
     rep_origin      3272..3349
                     /label=SV40_origin_of_replication
                     /note="SV40_origin_of_replication"
     primer_bind     complement(3756..3773)
                     /label=M13R
                     /note="M13R"
     promoter        complement(3764..3804)
                     /label=trc_promoter
                     /note="trc_promoter"
     primer_bind     complement(3769..3792)
                     /label=M13R-48
                     /note="M13R-48"
     primer_bind     complement(3770..3793)
                     /label=RV_M
                     /note="RV_M"
     gene            complement(3779..3801)
                     /label=lac_operator
                     /note="lac_operator"
     promoter        complement(3806..3835)
                     /label=lac_promoter
                     /note="lac_promoter"
     primer_bind     complement(4002..4018)
                     /label=Rtw
                     /note="Rtw"
     rep_origin      complement(4144..4763)
                     /direction=LEFT
                     /label=pBR322_replication_origin
                     /note="pBR322_replication_origin"
     gene            complement(4918..5778)
                     /label=ampicillin_resistance_gene_ORF
                     /note="ampicillin_resistance_gene_ORF"
     promoter        complement(5820..5848)
                     /label=Amp promoter
                     /note="Amp promoter"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg acggttttgt ggtgcgcacc agcaagtatc agcaggacac catcgatgaa
      901 aaaatcgcgc gctttattta tgcgaccaac agcccgtttc gcattgtgaa caacaaacac
      961 tttatcgaca tggtggaaag cttacgcccg ggctatactc ctcctggtcg cgcagatata
     1021 ggtggtcgtc tgctggatac cgcctacgaa aaagaactgg aacagtgcgc gcgtaatacc
     1081 gaaggcaagt ttgccagctt aagcctggat ggctggagca acgtgcataa cgaccctgtg
     1141 atatgcgcct gtgtgaccac tgaagatggc gtggcgtatc tgagcgaaac catcgatacc
     1201 agcggcaatg cccataccgc cgaatacctg aaagaagtgg cggtgaaagc ggtgattaac
     1261 tgcgaacaga agtttaactg ccatgtgcgc agcttcgtga ccgataacgc agcgaacgtg
     1321 gcaaaaatgc gccgctatct ggaagaagaa acggaaggct gcagcctgat tacctatggc
     1381 tgcagcgcgc acttaatgca tctgctggcg aaggatttat caaccagccc gggcattaaa
     1441 gaaaacgtgg tggaggtggc gaaatacttc cgcaacaacc attttgccag cgcgagctta
     1501 aaacgtgcgg gcggcagcaa attaatactg ccgcaggatg tgcgttggaa cagcgtggtg
     1561 gattgcttcg aacagttcat taagaactgg cccatcctga tcaaaatctg cgaagagaac
     1621 cgcgataaaa ttgatggcag cattagcagc aaagtgagca acatcggcct gaagcgcaac
     1681 gtggaagata tgctgagcat tctgaaacct atcagcattg cgctgaacaa actgcaacgc
     1741 gactgctgtt gtattgcgga cgcggtggaa atttggaagg cgctgcagga aaccctgaaa
     1801 aaagaaatcc cgaaccagaa agtgaaactg caggcgatca agaaacgcat ggaccaggcg
     1861 ttaaccccgc ctcattttct ggccaacatc ctgaacccgc gctataaggg ccgttgtctg
     1921 accagcgaag aagatgcggc agcaatggcg tgggcgagcc aaaatcatag cagcgtgatg
     1981 ccggtgatta ttaactttcg cgccggcggc gaacctttta agcagtacat gtttaccgac
     2041 gatgtgctga acaaggtgac ccctctgaac tggtggaaaa gcctggcgcg ccacttagaa
     2101 accgagttcc tgcagttact gagccagctg tttaccgcag ttgcgagcag cgcaggtacc
     2161 gaacgcatct ttagcagctt cggcattatc catagcaagc tgcgtaaccg tctgggcgtt
     2221 gaaaaagcgg gcaagctggt gttcctgttt cagagcatga acaaaaacga tagcggcagc
     2281 ggcgaagacg agatttgatg agcggccgct cgagcatgca tctagagggc cctattctat
     2341 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     2401 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     2461 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     2521 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     2581 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     2641 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     2701 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     2761 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     2821 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2881 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2941 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3001 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3061 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3121 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3181 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3241 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3301 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3361 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3421 aaagctcccc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     3601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     3721 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     3781 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     3841 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     3901 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3961 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4021 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4081 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4141 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4201 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4261 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4321 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4381 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4441 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4501 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4561 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     4621 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4681 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4741 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4801 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     4861 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4921 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4981 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5041 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5101 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5161 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5221 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5281 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5341 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5401 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5461 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5521 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5581 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5641 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5701 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5761 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5821 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5881 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     5941 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     6001 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     6061 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     6121 cgcgatgtac gggccagata tacgcgtt
//
