LOCUS       Exported                6529 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2679
                     /codon_start=1
                     /label=hAT-13_AMi
                     /translation="MENENVELPGLGEEEAGSSLSRSLSPDMRSTMSSCSRQSSFLSSF
                     VDKMSKEDQEKADETLARAIFASGTPLSITENQYWKEHYKLIRPSYKLPSRYRLSHSLL
                     DREYDRVQTMVIQRIGQAESLTLMSDGWTDIQGNPLLNIMLATPEPIFLKAIATKSSRH
                     TGEYIAKLLSEEIESAGPSRVQALVTDNASNMKAAWQILKAKYPHLIVFGCLAHGLNLL
                     AKDIVSVNTMKTILANCKAIVKVFNNHHVANQTLKKLQKEKQGKESALLLPGETRWGSA
                     TKCLHSLLHTKNCLQCASIDDTVSQIIPVNIRKQILDNDVFWVRVQGAFNLLLPVSVAI
                     KKLEGDTPSLSNIPEIFRQLNENLIELLPSSPLSKKEVVVKQMFTKRSEFCSHPVHLAA
                     NLLDPQHRGQHLSEDELSTALNTIMEVAKNVPNIDEIAVMTDIAEYRAKEKLWSKDIIW
                     RAAENMSPLTWWKGYCNTRLLSRIALRILSIPPTAASCERNWKAFSSIKTKKRNRLTNE
                     CTAKLVSISQNLLLLENQSEAQLVKKMQCDDKLPLPIDSLVKPSFSERECGSSSSESDL
                     PQSDSEMELISSASDSTDSDSETGGAAMTSVEES"
     promoter        complement(2717..2735)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2761..2985
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3031..3459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3473..3802
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3653..3788
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3968..4089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4138..4154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4162..4178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4186..4216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4231..4252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4540..5128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5299..6159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6160..6264)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg agaacgaaaa cgtggagtta ccgggcctgg gtgaagaaga agcgggcagc
      901 agcttaagcc gtagcttaag cccggatatg cgctcaacca tgagcagctg ttcacgccag
      961 agcagctttc tgagcagctt tgtggataaa atgagcaaag aggatcagga gaaggcggat
     1021 gaaaccttag cgcgcgcgat ttttgcaagc ggcacccctt taagcatcac cgagaaccag
     1081 tactggaagg aacactataa gctgattcgc cctagctaca aactgccgag ccgttaccgt
     1141 ttaagccata gcctgctgga tcgcgaatat gaccgcgtgc agaccatggt tatccagcgc
     1201 atcggtcaag cagaaagcct gaccttaatg agcgatggct ggaccgatat acaaggcaac
     1261 ccgctgctga acattatgct ggcgaccccg gagcctatat tcctgaaagc gattgcgacc
     1321 aaaagcagcc gtcataccgg cgagtatatc gcgaaactgc tgagcgaaga gattgaaagc
     1381 gccggccctt cacgtgttca agcactggtg accgacaatg ccagcaacat gaaggccgcc
     1441 tggcagattc tgaaggcgaa atacccgcac ctgattgtgt ttggctgcct ggcccatggt
     1501 ttaaatctgc tggcgaagga tattgtgagc gtgaacacca tgaaaaccat tctggcgaac
     1561 tgcaaggcca ttgtgaaagt gtttaacaac catcacgtgg ccaaccagac cctgaagaag
     1621 ctgcagaaag aaaagcaggg caaagaaagc gctctgctgc tgcctggtga aactcgctgg
     1681 ggtagcgcaa ccaaatgtct gcatagcctg ctgcatacca aaaactgctt acagtgcgcg
     1741 agcattgacg ataccgtgag ccagatcatt cctgtgaaca tccgcaaaca gattctggac
     1801 aacgacgtgt tttgggtgcg tgttcagggc gcgtttaacc tgctgttacc ggtgagcgtg
     1861 gcgatcaaaa aactggaagg cgataccccg agcttaagca acatccctga gatttttcgc
     1921 cagctgaacg agaacctgat tgaactgctg cctagcagcc cgctgagcaa gaaagaagtg
     1981 gtggtgaagc agatgtttac caaacgcagc gagttttgca gccatcctgt gcatctggcg
     2041 gccaacttac tggatcctca gcatcgcggt caacacctga gcgaagatga actgagcacc
     2101 gccctgaaca ccattatgga agtggcgaag aacgtgccga acattgacga aattgcggtg
     2161 atgaccgaca tagcggagta tcgcgcgaaa gagaaactgt ggagcaagga tattatttgg
     2221 cgcgcggcgg agaatatgag ccctctgacc tggtggaaag gctactgcaa cacccgtctg
     2281 ctgagccgta ttgcgctgcg cattctgagc attcctccga ctgcggcgtc atgtgaacgt
     2341 aactggaaag cgttcagcag catcaaaacc aaaaaacgca accgcctgac caatgagtgt
     2401 accgcgaagc tggtgagcat tagccagaac ctgctgctgc tggagaacca aagcgaagcg
     2461 cagctggtga aaaaaatgca atgcgatgac aagctgccgc tgcctattga cagcctggtg
     2521 aagcctagct ttagcgaacg cgaatgcggt agcagcagca gcgaatcaga tctgccgcag
     2581 agcgatagcg agatggaact gattagcagc gccagcgata gcaccgacag cgatagcgaa
     2641 actggtggtg cagcgatgac cagcgtggaa gaaagctaat gagcggccgc tcgagcatgc
     2701 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     2761 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     2821 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     2881 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     2941 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3001 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     3061 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3421 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     3481 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3541 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3601 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3661 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3721 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     3781 tggaggccta ggcttttgca aaaagctccc cgaaatgacc gaccaagcga cgcccaacct
     3841 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3901 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3961 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4021 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4081 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     4141 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     4201 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     4261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4321 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4381 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4441 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5881 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     6301 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     6361 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     6421 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     6481 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
