LOCUS       Exported                7294 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3444
                     /codon_start=1
                     /label=hAT-12_AMi
                     /translation="MKRTYQSGASKRKETKEKKARDEKGRQTLDQFGWVVKGNKEVGSG
                     TGDAGGTSLAGDEEYSDHENITKEASSDVAGECENESGLVVQAEGDIASNEKNETIDLV
                     SGSAPELHQEMFSNDPATWGHLDRLQRDLIVSKGPPPKPASFPCDSSKWSFPTSVFCKT
                     LSNDESVKRDWLIWSHKAAGLFCFPCCLFRDQNKNQALYHSRFCHGGVNDNWRKLYDKV
                     KSHEANSGHLQHYLQWKDLANALSGKGAIDNALEKGIHSEKEKWRKVLRGLVDVTLFLA
                     EQNLPFRGSHSTLEHDDCGLFLSTVQFISKYNQDLNCHLETVTEHRMAGERMQAHYMSW
                     RSQNEYLEVCAKKVLNTILEEVRNARYYGIIVDGTPDVSHTEQLVFIMRYVHEKDGKWD
                     VQERFLKMVDCDKKKGVDIVEQIKKVLAECKVELQNCRGQGYDNASNMSGKYQGVKTRI
                     QDENPQALFSPCSAHSLNLCGTHAMEKSPEVKTYFGNVQKLYTVFSCSPARWKILQETA
                     GLSLHSVSTTRWSSRIDAVRPLVKNPKGMLDSLFKIEEELDLTAETQADVSGLIEWMQS
                     FEFILLTTVWYKILQSINIRSKLVQGSKLTMEEGVQHVKSLLEEIQRLNDSWPSLLTEA
                     KVIAEGLGITTEFKRTHRRIRRTKHFHNEPANTEHTISSPEDKFRVEVFITAMDMLLSD
                     LRSLAETTGKLSSLFSPILDPPAVAEDEEIAKFAESLSTTYPRDVNKDELQEELCLFYK
                     IRKDSNLCTSGGAMGTALELLNQIYGKGVESVFPQICICLRMLIALPVSAASGERTFSK
                     LKIIKNYLRSAIGEDRLNHLIMLSVENALAKQINYDDVIDDFASMKARKKYFT"
     promoter        complement(3482..3500)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3526..3750
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3796..4224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4238..4567
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4418..4553
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4733..4854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4903..4919)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4927..4943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4951..4981)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4996..5017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5305..5893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6064..6924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6925..7029)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga agcgcaccta tcagagcggt gcgagcaaac gcaaagagac taaggaaaaa
      901 aaagcgcgcg atgaaaaagg tcgccagacc ctggatcaat tcggctgggt ggtgaaaggc
      961 aacaaggaag tgggtagcgg taccggtgat gcaggtggta cctcactggc aggcgatgag
     1021 gaatatagcg atcacgagaa catcaccaaa gaagcgagca gcgatgttgc gggcgaatgc
     1081 gaaaatgaaa gcggcctggt ggtgcaagcg gagggtgata ttgcgagcaa cgaaaagaac
     1141 gaaaccattg acctggtttc aggcagcgcg cctgaactgc atcaggaaat gtttagcaac
     1201 gatcctgcga cctggggcca tttagatcgc ctgcagcgcg atctgattgt gagcaaaggc
     1261 ccgcctccta agcctgcaag cttcccgtgc gatagcagca agtggagctt tccgaccagc
     1321 gtgttttgca aaaccctgag caacgatgag agcgtgaagc gcgactggct gatttggagc
     1381 cataaggcgg caggcctgtt ttgttttccg tgctgcctgt ttcgcgacca gaacaaaaac
     1441 caggcgctgt atcatagccg cttttgccat ggcggcgtga atgacaattg gcgcaagctg
     1501 tacgataaag tgaaaagcca tgaagcgaac agcggccact tacagcatta cttacagtgg
     1561 aaagacctgg cgaatgcgtt aagcggcaag ggtgcgattg acaacgccct ggaaaaaggc
     1621 attcatagcg aaaaggagaa atggcgcaaa gtgctgcgtg gcctggttga tgtgaccctg
     1681 tttctggccg aacagaactt accgttccgc ggtagccata gcaccctgga acatgatgat
     1741 tgcggcctgt ttctgagcac cgtgcagttc atcagcaagt ataaccagga tctgaactgc
     1801 cacctggaaa ccgttaccga acaccgtatg gcaggcgaac gcatgcaagc gcattacatg
     1861 agctggcgca gccagaacga atatctggaa gtgtgcgcga aaaaggtgct gaacaccatt
     1921 ctggaagaag tgcgcaacgc gcgctattat ggcattatcg tggatggcac cccggatgtt
     1981 tcacataccg agcagctggt gtttattatg cgctacgtgc atgagaagga cggcaagtgg
     2041 gatgtgcagg aacgctttct gaaaatggtg gactgcgaca aaaaaaaggg cgtggatatt
     2101 gtggaacaga tcaagaaggt gctggcggaa tgcaaagtgg agctgcaaaa ctgtcgcggc
     2161 cagggctatg ataacgccag caacatgagc ggcaagtatc agggcgtgaa aacccgcatt
     2221 caggacgaaa atccgcaggc gctgtttagc ccttgtagcg cgcatagcct gaatctgtgc
     2281 ggtacccacg cgatggaaaa gagcccggaa gtgaaaacct attttggcaa cgtgcagaaa
     2341 ctgtataccg tgtttagctg cagccctgcg cgttggaaga tactgcaaga aaccgcgggc
     2401 ttaagcctgc atagcgtgtc aaccacccgt tggagcagcc gtattgacgc tgtgcgtcct
     2461 ctggtgaaga accctaaagg catgctggat agcctgttta agattgagga agaactggat
     2521 ctgaccgccg aaacccaagc agatgtgagc ggcctgattg aatggatgca gagctttgaa
     2581 tttatcctgc tgaccaccgt gtggtataaa attctgcaga gcatcaacat tcgcagcaag
     2641 ctggtgcaag gcagcaaact gaccatggaa gagggcgtgc agcatgtgaa aagcctgctg
     2701 gaagaaatcc agcgcctgaa tgatagctgg ccgagcttac tgaccgaagc gaaagtgatt
     2761 gccgaaggcc tgggcattac cactgagttc aagcgcaccc atcgtcgtat tcgccgcacc
     2821 aaacatttcc ataacgaacc tgccaacacc gagcatacta tcagcagccc ggaagataaa
     2881 tttcgcgtgg aggtgtttat taccgccatg gatatgctgc tgagcgatct gcgtagctta
     2941 gcggaaacca ccggtaaact gagcagcctg tttagcccta tcctggaccc tcctgcagtt
     3001 gcagaggatg aagaaattgc gaagttcgcg gaaagcctga gcactaccta tcctcgcgac
     3061 gtgaacaaag acgaactgca ggaggaactg tgcctgtttt acaaaattcg caaggacagc
     3121 aacttatgta ccagcggcgg tgcaatgggt accgcgttag aactgctgaa ccagatctac
     3181 ggcaaaggcg tggaatcagt gttcccgcag atttgcattt gcctgcgcat gctgatcgcg
     3241 ttacctgtta gcgcagcgag cggtgaacgt accttcagca agctgaaaat catcaagaac
     3301 tatctgcgca gcgcgatcgg tgaagatcgt ctgaaccacc tgattatgct gagcgtggag
     3361 aacgcactgg cgaaacagat taactacgac gatgtgatcg atgattttgc gagcatgaaa
     3421 gcgcgcaaaa agtacttcac ctgatgagcg gccgctcgag catgcatcta gagggcccta
     3481 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     3541 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     3601 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     3661 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     3721 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     3781 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     4141 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     4201 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     4261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4321 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4381 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4441 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4501 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     4561 ttgcaaaaag ctccccgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4621 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4681 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4741 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     4801 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     4861 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4921 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4981 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5041 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5101 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6361 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6661 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7021 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     7081 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     7141 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
     7201 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
     7261 ctgcttcgcg atgtacgggc cagatatacg cgtt
//
