LOCUS       Exported                6490 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2640
                     /codon_start=1
                     /label=Mariner-3_AMi
                     /translation="MASNMLFTASKSKGEKRKRVVLTLMQKIDICRCLEKGENRNVLMK
                     EFSVGSSTIYDIKAQKEQLLKFVSSSETSKAVEKCRTLHKPKLEQLDSVLYEWFSLKRS
                     EGAPVSGPMLIEKAKDFYKQMDLTEPCAFSAGWLSCFKLRHGIRRLDVSGEIKSADHEA
                     AEKYCGFFNKLVAEHDLSPDQIYNTDETGLFWRCLPNSTLAGAGESSAAGFKQNKDRIT
                     VLTCANAGGTHKVKLLVVGKCNRPRAFKGITHLPVVYKAQSNAWMDKGIFYDWFHHVFV
                     PSVKEHFRKIGKPQDSKAILLLDNCRAHPPECELVSGNIFTIFLPANVTSLIQPMDQGI
                     IQNMKCYYRRDFLRKLINHEGTIQNFQSLYNIKDAIFNVACAWNSVKSKTLRRAWRKLW
                     PSVMFAEGSSDEEDFEGFNVRHRKNTIAQILEVVKDAPSTNPVNKLKESEIEEWVEADK
                     EVEHVFSDAEIIESVVNPEKSKVTEKDSEEEDFSEEEKISWASAASYIDNFIKFAERQS
                     CYSAQEVMQLHIIHSDFMQKRQAASRQADIRDFFKKASRACHKASTESAPSPSPSTSTI
                     DVDDSEALQDPQVPLESPKRQ"
     promoter        complement(2678..2696)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2722..2946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2992..3420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3434..3763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3614..3749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3929..4050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4099..4115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4123..4139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4147..4177)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4192..4213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4501..5089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5260..6120)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6121..6225)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg ccagcaacat gctgttcacc gccagcaaaa gcaagggcga aaaacgcaaa
      901 cgcgtggtgt taaccctgat gcagaagatt gatatttgcc gctgcctgga gaaaggcgaa
      961 aaccgcaacg tgctgatgaa agaatttagc gtgggcagca gcaccattta cgacatcaag
     1021 gcgcagaaag aacagctgct gaagtttgtg agcagcagcg aaaccagcaa agcggtggag
     1081 aaatgccgta ccctgcacaa gccgaaactg gagcagttag acagcgtgct gtatgagtgg
     1141 tttagcctga agcgtagcga aggcgcacct gttagcggtc cgatgctgat tgagaaagcg
     1201 aaggacttct acaaacagat ggacctgacc gaaccttgtg cgtttagcgc aggttggctg
     1261 agctgtttta aactgcgcca tggcatacgt cgtctggatg tgagcggcga gatcaaaagc
     1321 gcggatcacg aagcggcgga gaagtattgc ggcttcttta acaaactggt ggcggaacac
     1381 gatctgagcc ctgatcagat ttacaacacc gatgaaaccg gcctgttttg gcgttgcctg
     1441 cctaacagca ccttagcggg tgcaggcgaa tcaagcgcag cgggtttcaa gcagaacaag
     1501 gatcgcatca ccgtgctgac ctgtgcaaat gcaggtggca cccacaaagt gaaactgctg
     1561 gtggtgggca aatgtaatcg tccgcgcgcg tttaaaggca tcacccatct gccggtggtg
     1621 tacaaagcgc agagcaacgc gtggatggat aagggcatct tctatgattg gttccatcat
     1681 gtgtttgtgc cgagcgtgaa ggaacacttc cgcaaaatcg gcaaaccgca ggacagcaaa
     1741 gcgattctgc tgctggataa ctgccgtgcg catcctcctg aatgcgaact ggtgagcggc
     1801 aacatcttta ccatctttct gccggcgaac gtgacctcac tgattcagcc tatggatcag
     1861 ggcattatcc agaacatgaa atgctactac cgccgcgatt ttctgcgcaa actgattaac
     1921 cacgaaggca ccattcagaa cttccagagc ctgtacaaca ttaaggacgc gatttttaac
     1981 gtggcgtgcg cctggaatag cgtgaagagc aagaccttac gtcgcgcgtg gcgtaaatta
     2041 tggccgagcg tgatgtttgc ggagggcagc agcgatgaag aagatttcga aggcttcaac
     2101 gtgcgccatc gcaaaaacac catcgcgcag attctggagg tggtgaagga tgcgccgtca
     2161 accaaccctg tgaacaaact gaaggaaagc gagattgaag aatgggtgga agcggacaag
     2221 gaagtggagc acgtgttcag cgatgcggag atcatcgaaa gcgtggtgaa cccggaaaaa
     2281 agcaaggtga ccgaaaagga tagcgaggaa gaggatttta gcgaggaaga gaaaatcagc
     2341 tgggcaagcg cggcgagcta tattgataac tttatcaagt ttgcggagcg ccagagctgt
     2401 tatagcgcgc aggaagtgat gcagctgcat attatccaca gcgactttat gcagaaacgc
     2461 caggcggcat cacgtcaggc agatatccgc gacttcttta agaaagcgag ccgcgcctgt
     2521 cacaaagcaa gcaccgaaag cgcgccttca ccttcaccta gcaccagcac cattgatgtg
     2581 gacgatagcg aggctctgca ggatcctcaa gtgcctctgg aaagcccgaa gcgccagtaa
     2641 tgagcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
     2701 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     2761 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     2821 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     2881 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     2941 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     3001 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3061 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3121 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3181 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3241 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3301 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3361 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3421 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     3481 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     3541 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     3601 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3661 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     3721 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgaaatgac
     3781 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     3841 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     3901 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     3961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     4021 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     4081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6241 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     6301 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     6361 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     6421 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     6481 tatacgcgtt
//
