LOCUS       Exported                6547 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2697
                     /codon_start=1
                     /label=hAT-3_AMi
                     /translation="MCKFVQRCKLSGRIDSVLSAQFEKECSYWRKVLRRVVATVKLLSS
                     LGLPLRGHDESSLSNRKGNFLTCLEYLSEFDDFLKEHLKNYQYCGSGKTNFLTHQTYDE
                     FITIMAERLRNQFTDEVKDAKYYSIIVDSTPDVSHVDQLTLILRYVTGNGEVVERFLCF
                     VPLKSHTSECLETTVLEIIANLGLDIKNCHGQSFDNAANMAGKYSGLQARLNNASPSAL
                     FIPCSSHSLNLICNAAAESCEGATHFFYFVQNVYAFFSVSTHRWSMLQSHLEQANEKHL
                     TVKRICDTRWSARADAVLALKMSYDDIKKTLLDIATSNSEKPRAKTEAKSLAEKFEKYD
                     IAVFTLLWNRLLQRINAVSKLLQKVDTNLLNAAQLLASVKSFVMEVRNDYSSVEAEALT
                     LTENIGSSDISDEKRKKSRKLFFDETRDNEINLKGKEKIIVETVNVICDTIIVQLQSRG
                     KSLKKTVNLFCVFFDRSMDENAKSHYSKKLSEFYREDIDTNLFEDEVKHFIHFIENDEV
                     CASRSPVDLYRLVRDGLQATFPNVETILKIFLTIPVTNASGERSFSVLKRVKNYLRNTM
                     SQEHLTSSAILTIESASLQNITYDDLIDDFVKKKCRKKAI"
     promoter        complement(2735..2753)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2779..3003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3049..3477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3491..3820
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3671..3806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3986..4107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4156..4172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4180..4196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4204..4234)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4249..4270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4558..5146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5317..6177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6178..6282)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgt gcaagtttgt gcagcgctgc aaattatcgg gccgcattga ttcagttctg
      901 agcgcgcagt ttgaaaaaga atgcagctac tggcgcaagg ttctgcgtcg tgtggtggcg
      961 accgttaaac tgctgagcag cttaggtctg cctttacgcg gccacgatga aagctcactg
     1021 agcaaccgca aaggcaactt cctgacctgc ctggaatacc tgagcgagtt tgatgacttc
     1081 ctgaaagagc atctgaagaa ctaccagtat tgcggcagcg gcaagaccaa ctttctgacc
     1141 catcagacct acgatgagtt tatcaccatc atggcggagc gtctgcgtaa ccagttcacc
     1201 gatgaagtga aagacgcgaa atactacagc attattgtgg acagcacccc tgatgtgagc
     1261 catgtggatc agctgaccct gattctgcgt tacgtgaccg gtaacggcga agtggtggaa
     1321 cgctttctgt gctttgtgcc tctgaagagc cataccagcg agtgtctgga aaccaccgtg
     1381 ctggagatta ttgcgaacct gggcctggac attaagaact gccatggcca gagctttgac
     1441 aacgccgcga acatggcggg taaatatagc ggtctgcagg cgcgtttaaa caatgcaagc
     1501 ccgagcgcgt tatttattcc ttgcagcagc catagcctga acctgatttg taacgcggcg
     1561 gcggaatcat gtgaaggcgc aacccacttc ttctattttg tgcagaacgt gtatgcgttt
     1621 tttagcgtga gcacccatcg ctggagcatg ctgcaaagcc atctggagca ggccaacgaa
     1681 aaacacctga ccgtgaagcg catatgcgat acccgttggt cagcgcgtgc ggatgcggtt
     1741 ttagcgctga aaatgagcta cgacgacatc aaaaaaaccc tgctggatat cgcgaccagc
     1801 aactcagaaa agccgcgcgc gaaaaccgaa gcgaaaagcc tggccgagaa gtttgagaaa
     1861 tatgacatcg ccgtgtttac cctgttatgg aaccgcctgc tgcaacgcat taacgcggtg
     1921 agcaaactgc tgcagaaagt ggataccaac ctgctgaatg cggcgcaatt actggcgagc
     1981 gtgaaaagct tcgtgatgga ggtgcgcaac gattatagca gcgtggaagc cgaagcgtta
     2041 accctgaccg aaaacattgg cagcagcgac attagcgacg aaaaacgcaa gaaaagccgc
     2101 aaactgtttt tcgatgagac tcgcgataac gaaattaacc tgaagggcaa agagaagatt
     2161 atcgtggaaa ccgtgaacgt gatttgcgat accattattg tgcagctgca gagccgtggc
     2221 aagagcctga aaaaaaccgt gaacctgttt tgcgtgtttt tcgatcgcag catggatgaa
     2281 aacgcgaaaa gccactacag caaaaaactg agcgagtttt accgcgaaga tattgatacc
     2341 aacctgtttg aggatgaggt gaagcacttc atccacttca ttgaaaacga cgaggtgtgt
     2401 gcgtcacgta gccctgtgga tttatatcgc ctggtgcgcg atggtctgca agccaccttt
     2461 ccgaacgtgg aaaccatcct gaaaatcttt ctgaccattc ctgtgaccaa cgcatcaggc
     2521 gaacgcagct tttcagtgct gaagcgcgtg aagaactacc tgcgcaacac catgagccag
     2581 gaacatctga ccagcagcgc gattctgacc attgaaagcg ccagcctgca gaacattacc
     2641 tatgacgatc tgatcgacga tttcgtgaag aaaaagtgcc gcaagaaggc gatctaatga
     2701 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     2761 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2821 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2881 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2941 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     3001 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3061 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3121 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3181 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3241 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3301 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3361 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     3481 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3541 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3661 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3721 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     3781 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg aaatgaccga
     3841 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3901 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3961 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     4021 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4081 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4141 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4201 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4261 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4321 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5281 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5341 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5401 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5461 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5521 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5581 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5641 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5701 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5761 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5821 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5881 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5941 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6001 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6061 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6121 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6181 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6241 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6301 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     6361 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     6421 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     6481 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     6541 acgcgtt
//
