LOCUS       Exported                6721 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2871
                     /codon_start=1
                     /label=hAT-8_AMi
                     /translation="MSKRKREEGECSGPAKKKSCSRFKEDWLKEMFETDMPHASGRQRV
                     SLGDIFQYRESGDVLCTICVRAGANSEWTTRKKWGDWKLDYLKRHLTHKIHTDSVMKLR
                     NQSRGILKSLFSETRETRENRIELSERKRSNADEVKILIDNVLLTVKLNLSMLSVQEIH
                     SHVGKYVKIPESWRSKNYAFEFIDCINSVIHQEVLNEMRTSPFHTLIVDESTDISVSKM
                     LILYVKFRTMDDFTHQTRFAGILKLTACDSSAIVAAIRQFYVDSNLDMQKMVMFTSDGA
                     SVMLGKRNGVAAVLRQSIPHLLEQHCVAHREDLGIDDAWKDVPLMRCIETLLRTIYTLF
                     SRSSVKKSKFEELSKAAECETVSFRPLNEVRWLSRHFAVSALMKNYTVLMDYCAQQVAE
                     DNDPIHKYCLDKLRKPEYHVALAILNDVLGELAELCRMLQRSSLTTVEAFQFAKARIAK
                     LRSQYLGETVHFSEDVQTLLSSFESPVDTTSVIQFIEHTCDHMDNRFPEGELKEWSIFD
                     KDSFSSSSNRNDFSFGHEELKTLATRYSALLNKSVETFKKDLCKQYVDFKLTVSEKMKT
                     GAIKTFEDMVTYILQQEQFSELSLLVDICATFQASSADCERGFSLMNAIKTKSRNRLEV
                     DNLDKLMRIKLHLASGKTVNLDTVYSVWKTEKGRRESLW"
     promoter        complement(2909..2927)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2953..3177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3223..3651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3665..3994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3845..3980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4160..4281
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4330..4346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4354..4370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4378..4408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4423..4444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4732..5320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5491..6351)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6352..6456)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcaagcgcaa acgcgaagaa ggtgagtgta gcggcccggc caagaaaaaa
      901 agctgcagcc gctttaaaga agactggctg aaggagatgt tcgaaaccga tatgccgcat
      961 gcaagcggtc gtcagcgtgt tagcctgggc gacatttttc agtatcgcga gagcggcgat
     1021 gttctgtgca ccatttgtgt gcgtgcgggc gcgaatagtg aatggaccac ccgcaaaaag
     1081 tggggcgatt ggaagctgga ttatctgaaa cgccacctga cccataaaat tcataccgac
     1141 agcgtgatga agctgcgcaa tcagagccgc ggcattctga aaagcctgtt cagcgagact
     1201 cgtgaaacgc gtgaaaaccg catcgagctg agcgaacgca aacgcagcaa tgcggacgag
     1261 gtgaaaatcc tgatcgacaa cgtgctgctg accgtgaaac tgaacctgag catgctgagc
     1321 gtgcaggaaa tccatagcca tgtgggcaag tatgtgaaaa ttccggaaag ctggcgcagc
     1381 aagaactatg cgtttgagtt catcgactgc attaacagcg tgattcatca ggaagtgctg
     1441 aacgagatgc gcaccagccc ttttcacacc ctgattgtgg atgagagcac cgatatcagc
     1501 gtgagcaaaa tgctgattct gtacgtgaag ttccgcacca tggatgactt tacccaccag
     1561 actcgctttg cgggcattct gaaactgacc gcgtgtgata gcagcgcgat agttgcagcg
     1621 attcgccagt tctatgtgga tagcaacctg gatatgcaga agatggtgat gtttaccagc
     1681 gatggcgcga gcgttatgtt aggcaagcgt aacggtgtgg ccgcagtttt acgtcagagc
     1741 attcctcacc tgctggaaca gcattgtgtg gcgcatcgcg aagatctggg cattgatgat
     1801 gcgtggaagg acgttcctct gatgcgctgc attgaaacgt tactgcgcac catctatacc
     1861 ctgttcagcc gcagcagcgt gaagaaaagc aaatttgagg aactgagcaa agccgcggaa
     1921 tgcgaaaccg ttagctttcg cccgctgaac gaagttcgct ggctgagccg ccattttgca
     1981 gttagcgcgc tgatgaaaaa ctataccgtg ctgatggact attgcgcgca gcaggtggca
     2041 gaagataacg acccgattca taaatactgc ctggataaac tgcgcaagcc ggaatatcat
     2101 gtggcgctgg cgattctgaa tgatgtgctg ggcgaactgg cagaattatg ccgcatgctg
     2161 caacgcagca gcttaaccac cgtggaagcc tttcagtttg cgaaggcgcg cattgcgaaa
     2221 ctgcgcagcc aatatctggg cgaaaccgtg cattttagcg aagatgtgca gaccctgctg
     2281 agcagctttg agagccctgt ggataccacc agcgtgattc agtttattga gcatacctgc
     2341 gatcacatgg acaaccgctt tccggaaggc gaactgaaag agtggagcat tttcgataag
     2401 gatagcttca gcagcagcag caaccgcaac gatttcagct tcggccatga ggagctgaaa
     2461 accctggcga cccgctattc agcgctgctg aacaagagcg tggaaacctt taaaaaggac
     2521 ctgtgcaagc agtatgtgga ctttaaactg accgtgagcg aaaaaatgaa aaccggcgcg
     2581 attaaaacct ttgaagacat ggtgacctat attctgcagc aggaacagtt tagcgagctg
     2641 agcctgctgg tggatatttg cgcgaccttt caagcgagca gcgcggattg tgaacgcggc
     2701 tttagcctga tgaacgcgat caaaaccaag agccgcaacc gcctggaagt ggataacctg
     2761 gacaaactga tgcgcattaa actgcacctg gcgagcggta aaaccgtgaa cctggatacc
     2821 gtgtatagcg tgtggaaaac cgagaaaggc cgccgtgaga gcttatggta atgagcggcc
     2881 gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     2941 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3001 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3061 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3121 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     3181 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     3241 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3301 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3361 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3421 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3481 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3541 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3601 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     3661 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3721 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3781 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3841 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3901 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3961 aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgaaatga ccgaccaagc
     4021 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     4081 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     4141 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     4201 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4261 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     4321 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4381 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4441 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4501 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4561 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4621 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4681 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4741 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4801 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4861 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4921 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4981 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5041 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5101 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5161 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5221 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5281 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5341 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5401 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5461 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5521 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5581 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5641 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5701 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5761 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5821 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5881 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5941 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6001 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6061 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6121 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6181 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6241 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6301 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6361 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6421 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6481 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     6541 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     6601 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     6661 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     6721 t
//
