LOCUS       Exported                6091 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2241
                     /codon_start=1
                     /label=Mariner-2_PM
                     /translation="MAPKKVSGSDKPKRKVLRTTIELRKEIIEKAERGVRVTDISKEYG
                     MPKSTICTILKNKEAIKEANVAKGVSSLSKQRTKAIEEVEKLLLIWINEKQLAGDSVSE
                     AMICEKAKQLHSDLLKNTPGTSDDAGPSDIFKASRGWFDKFKKRSGIHSVVRHGEAASA
                     NKEAAKNFVGEFKEFVDAEGFIPQQVFNCDETGLFWKKMPRRTYITQEEKALPGHKPMK
                     DRLTLLLCGNASGDFKVKPLLVYHSENPRVFKKNNVMKNKLHVMWRANSKAWVTRQFFI
                     EWVHEVFGPSVKKYLQEKQLPLKALLLMDNAPAHPPGLEESCRGSFLITVKFLPPNTTP
                     LIQPMDQQVISNFKKLYTKALFQRCFEVTSDTELTLREFWKNHFNILHCLSLIDKAWGE
                     VSSRTMNSAWRKLWPDCVAERDFEGFEPEPETEPVVENIVSLGKSMGLEVDDEMLRSWW
                     RTTAQN"
     promoter        complement(2279..2297)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2323..2547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2593..3021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3035..3364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3215..3350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3530..3651
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3700..3716)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3724..3740
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3748..3778)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3793..3814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4102..4690)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4861..5721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5722..5826)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg cccctaaaaa agtgagcggc agcgataaac cgaaacgcaa agtgctgcgc
      901 accaccattg agctgcgcaa agagattatc gaaaaggcgg aacgtggtgt gcgtgtgacc
      961 gatatcagca aggaatatgg catgcctaaa agcaccattt gcaccatcct gaagaacaaa
     1021 gaggccatta aggaagcgaa cgtggcgaaa ggtgttagca gcctgagcaa gcaacgcacc
     1081 aaagcgatcg aggaagtgga aaaactgctg ctgatctgga ttaacgaaaa acagctggcg
     1141 ggcgatagcg tttcagaggc gatgatctgc gaaaaggcga agcagctgca tagcgacctg
     1201 ctgaaaaata ccccgggcac ctcagatgat gcgggcccga gcgacatttt taaagcgagc
     1261 cgcggctggt tcgataaatt caaaaagcgc agcggcattc atagcgtggt gcgccatggt
     1321 gaagcggcga gcgcgaataa agaagccgcc aagaacttcg tgggcgagtt taaagagttt
     1381 gtggatgcgg aaggctttat ccctcagcag gtgtttaact gcgatgaaac cggcctgttt
     1441 tggaagaaaa tgccgcgccg cacctatatt acccaggaag aaaaagcgct gccgggccat
     1501 aaacctatga aggaccgcct gaccctgctg ttatgtggca acgcgagcgg tgacttcaaa
     1561 gtgaaaccgc tgctggtgta ccatagcgaa aaccctcgcg tgtttaaaaa gaacaacgtg
     1621 atgaagaaca agctgcatgt gatgtggcgc gccaatagca aagcatgggt gacccgccag
     1681 ttttttattg aatgggtgca tgaggtgttt ggcccgagcg tgaagaaata cctgcaggaa
     1741 aagcagctgc ctctgaaggc gctgctgtta atggataacg cccctgcaca tcctcctggc
     1801 ttagaagaat catgccgcgg cagctttctg attaccgtga agttcctgcc tccgaacacc
     1861 acccctctga ttcagcctat ggaccagcag gtgatcagca actttaaaaa gctgtacacc
     1921 aaagcgctgt tccagcgctg tttcgaagtg accagcgaca ccgaattaac cctgcgcgag
     1981 ttctggaaga accacttcaa cattctgcat tgcctgagcc tgatcgataa ggcgtggggc
     2041 gaagtttcaa gccgcaccat gaatagcgcg tggcgtaaac tgtggccgga ttgtgttgcg
     2101 gagcgcgatt ttgaaggctt tgaaccggaa cctgagactg aaccggtggt ggaaaacatt
     2161 gtgagcctgg gcaagtcaat gggcctggaa gtggatgatg aaatgctgcg cagctggtgg
     2221 cgtaccaccg cccagaacta atgagcggcc gctcgagcat gcatctagag ggccctattc
     2281 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     2341 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2401 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2461 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     2521 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     2581 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     2641 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     2701 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     2761 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2821 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2881 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2941 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3001 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3061 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3121 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3181 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3241 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3301 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3361 caaaaagctc cccgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     3421 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     3481 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     3541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     3661 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     3721 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     3781 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     3841 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3901 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     3961 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4021 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     4081 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4141 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4201 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4261 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4321 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4381 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4441 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4501 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     4561 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     4621 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     4681 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     4741 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     4801 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     4861 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4921 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4981 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     5041 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     5101 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     5161 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     5221 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     5281 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     5341 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     5401 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     5461 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     5521 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     5581 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5641 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5701 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5761 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     5821 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     5881 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     5941 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     6001 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     6061 cttcgcgatg tacgggccag atatacgcgt t
//
