LOCUS       Exported                6487 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2637
                     /codon_start=1
                     /label=Mariner-1_Crp
                     /translation="MAEKHKSGGSDVSSSKKRRTITMETKMEIIKRSEKGETPTEMGRA
                     LDIPRTTIVTILKDKARIQEHVKDSAPMHSTVITKQRVGLIAQVEKLLIIWLEDQNKRR
                     APVSLGIIQEKARSLYDDLKKQQGESSNAEPFTASKGWFMRFKARANLHNIKVSGEAAS
                     ADEEAARAFPETLAEIIEESGYCAQQVFNVDETGLFWKKMPSRTYIAKEEKSMPGYKAA
                     KDRLTLLLGANAAGDFKLKPLLVYRSENPRAFKGHSKAFLPVIWKSNPKAWVNKNIFED
                     WFNHHFVPSVRDYCSKNNLAFKALLILDNAPGHPTILDDMHPDIKVVFLPPNTTSLLQP
                     TDQGVIASFKAYYLRRTFAQAIRAIEKEGGPTLKEFWKGFNIYHAVKNIGEAWNEVKQS
                     NLNGVWRKLCPKFVSDFQGFTDTVEEVTKNVVEMGKELNLDVAPEDVDELLASHSEELT
                     NEDLIELEQQKVAEEEDAPTVEETPPRKVLTTKVLAEAFQHLEAAMSLFEEHDPDIERS
                     ASVNRGISSMYSCYREIYKQKKRTSVQTSLRTFFKKADKTPGKPAAKTPEKTPAKIPSK
                     SPAKNPSKSPAKSPQRSPSK"
     promoter        complement(2675..2693)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2719..2943
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2989..3417
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3431..3760
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3611..3746
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3926..4047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4096..4112)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4120..4136
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4144..4174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4189..4210
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4498..5086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5257..6117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6118..6222)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgg cggagaagca taaaagcggc ggcagcgatg ttagcagcag caaaaagcgc
      901 cgcaccatca ccatggaaac caagatggag attattaagc gcagcgagaa aggtgaaacc
      961 ccgaccgaaa tgggtcgcgc gttagatatt cctcgcacca ccattgtgac catcctgaaa
     1021 gataaagcgc gcattcagga acatgtgaaa gatagcgccc ctatgcatag caccgtgatt
     1081 accaagcagc gcgtgggttt aatcgcccaa gtggaaaagc tgctgattat ttggctggag
     1141 gatcagaaca aacgccgtgc acctgtgagc ctgggtatta ttcaggagaa ggcccgcagc
     1201 ctgtatgatg acctgaaaaa acagcagggc gagagcagca acgcggaacc ttttaccgcc
     1261 agcaaaggct ggtttatgcg ctttaaggcg cgcgccaacc tgcataacat caaagtgagc
     1321 ggtgaagcag cgagcgcaga tgaagaagcg gcgcgtgcct ttcctgaaac cctggccgaa
     1381 atcattgagg aaagcggcta ctgtgcacag caggtgttca acgtggacga aaccggcctg
     1441 ttttggaaga agatgccgag ccgcacctac atcgccaaag aagaaaagag catgcctggc
     1501 tacaaagccg cgaaagatcg cctgacctta ctgctgggtg caaatgcagc gggcgatttt
     1561 aaactgaagc ctctgctggt ttaccgcagc gaaaacccgc gtgcattcaa gggtcatagc
     1621 aaggcgtttc tgcctgtgat ctggaaaagc aacccgaaag cgtgggtgaa caagaacatt
     1681 ttcgaggact ggttcaacca ccactttgtg cctagcgtgc gcgactattg cagcaaaaac
     1741 aacctggcgt tcaaagcgct gctgattctg gataacgccc ctggccatcc gaccattctg
     1801 gatgacatgc acccggatat caaagtggtg ttcctgcctc ctaacaccac cagcttactg
     1861 cagccgaccg atcagggcgt gattgcgagc tttaaagcgt actacctgcg ccgcaccttt
     1921 gcacaagcga tacgcgcgat tgaaaaggaa ggcggcccga ccttaaaaga attttggaag
     1981 ggcttcaaca tttatcacgc ggtgaagaac attggcgaag cgtggaacga agtgaaacag
     2041 agcaacctga acggcgtgtg gcgtaaactg tgccctaaat ttgtgagcga ctttcagggc
     2101 ttcaccgata ccgttgagga ggtgaccaaa aacgtggtgg aaatgggcaa agagctgaac
     2161 ttagatgtgg ccccggaaga tgtggatgag ttactggcga gccacagcga agagctgacc
     2221 aacgaagatc tgattgagct ggaacagcag aaggtggcgg aagaagaaga tgccccgacc
     2281 gtggaagaaa cccctcctcg caaagtgctg accaccaaag ttctggcgga ggcgtttcaa
     2341 cacctggaag cggcgatgag cctgtttgaa gaacatgatc cggacattga acgcagcgcg
     2401 agcgttaatc gtggcatcag cagcatgtat agctgctacc gcgagattta caaacagaaa
     2461 aaacgcacca gcgtgcaaac tagcctgcgc acctttttta aaaaggcgga taaaaccccg
     2521 ggtaaaccgg cagcaaaaac cccggaaaaa acccctgcga agattcctag caagagcccg
     2581 gcaaaaaacc cgtcaaagag ccctgcgaaa agccctcaac gtagcccgag caaatgatga
     2641 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     2701 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     2761 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     2821 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     2881 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     2941 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     3001 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3061 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3121 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3181 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3241 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     3301 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     3361 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     3421 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3481 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3541 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3601 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3661 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     3721 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg aaatgaccga
     3781 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3841 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3901 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     3961 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4021 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     4081 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4141 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4201 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4261 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4321 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4381 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4441 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4501 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4561 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4621 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4681 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4741 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4801 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4861 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4921 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4981 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5041 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5101 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5161 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5221 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5281 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5341 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5401 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5461 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5521 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5581 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5641 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5701 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     5761 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     5821 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     5881 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     5941 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6001 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6061 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6121 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6181 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6241 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     6301 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     6361 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     6421 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     6481 acgcgtt
//
