LOCUS       Exported                6892 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..3042
                     /codon_start=1
                     /label=hAT-12_Crp
                     /translation="MSESNRVTLQKVRIESEASESIWGDSEGLELHTPQSSPRGRSPSL
                     EGTSEEVVGAGGSAPPVVVPLPGEEGDKAGSPPSTQKRGGSVVWDHFEVADDPRYATCQ
                     HCRRQISRGKEVKHFATTAMLLHLRRQHPLALVPPQPGTSGSVPKKKSPSRSKAPVPKK
                     QRQATLDQWGKGGDKGGRVPKASEVTQSIGEMLALDGQPFSFVERPGFRRLMALVAPSY
                     QVPARTTFSRTVVPSLYEACREYLREELRKAGPQVALHFTSDIWSSRGGDHAYLSLTGH
                     WCDQSGRRWALLQAEVMDESHTAREIMVAMNRMVQGWLVGQGELTRGFMVTDNGANMVK
                     AVRDANFVGIRCVAHKFHLVVRDALEGDRAPGDGAVTTSQLISKCRKVAGYFHRSIKGG
                     KMLRDKQAELSIPQHKIMQDVETRWNSTYLMLERLVEQQKAIHEMALVGEIGISGPLNK
                     TEWDTISQILVVLKPFLEATESLSAGDALLSQVIPVVKELENEMEKFQGIDVPGWGKPL
                     SPEVQALVKQLKEGIKKRLDPLRSSTVHVLAGMCDPRVKGSVCSSKTLDHWTEVLVNRV
                     REAEGQRRGDVEEGDPLSRASTPTTSQSPPPQPLPMWAKGMASMLGSRGTRPHHQAGSA
                     EASVATYLAEDVEPLHCDPLAYWATRSQVWQDLATVAREHLSCPPTSVPSERVFSIAGD
                     VVTPHRTSLDPGLVEQLVFLKVNLPLLGFPKLHVQTE"
     promoter        complement(3080..3098)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3124..3348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3394..3822
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3836..4165
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4016..4151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4331..4452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4501..4517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4525..4541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4549..4579)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4594..4615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4903..5491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5662..6522)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6523..6627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatga gcgaaagcaa ccgcgtgacc ctgcagaaag tgcgcattga aagcgaagcg
      901 agcgaaagca tttggggcga tagcgaaggc ctggaactgc ataccccgca gagctcacct
      961 cgtggtcgta gccctagctt ggaaggcact agcgaagaag ttgtgggcgc gggtggtagc
     1021 gcgccgcctg ttgttgtacc tttacctggt gaagaaggtg ataaagcggg tagccctccg
     1081 agcacccaga aacgcggcgg cagcgttgta tgggatcatt ttgaagtggc ggatgatccg
     1141 cgctatgcga cctgccaaca ttgccgccgt caaattagcc gcggcaaaga agttaaacat
     1201 tttgcgacca ccgcgatgct gctgcatctg cgccgtcaac atcctctggc gttagtgccg
     1261 ccgcaacctg gtaccagcgg tagcgttcct aaaaagaaaa gcccgagccg cagcaaagcg
     1321 ccggtgccta aaaaacagcg ccaggcgacc ttagatcagt ggggcaaagg cggcgataaa
     1381 ggcggccgtg ttcctaaagc gagcgaagtt acccaaagca ttggcgaaat gctggcgctg
     1441 gatggccagc cgtttagctt tgtggaacgc ccgggttttc gccgcttaat ggcgttagtg
     1501 gcgccgagct atcaagtgcc ggcgcgtact acttttagcc gtaccgttgt gccgagctta
     1561 tatgaagcgt gccgcgaata tctgcgcgaa gaactgcgca aagcgggccc gcaagttgcg
     1621 ttacatttta ccagcgatat ttggagcagc cgcggcggcg atcatgcgta tttaagcctg
     1681 accggtcatt ggtgcgatca gagcggccgt cgttgggcgt tgttacaagc ggaagttatg
     1741 gatgaaagcc ataccgcgcg cgaaattatg gtggcgatga accgcatggt gcagggctgg
     1801 ttagttggcc agggtgaact gacccgtggt tttatggtga ccgataacgg cgcgaacatg
     1861 gtgaaagcgg tgcgcgatgc gaattttgtg ggcattcgct gcgtggcgca taaatttcat
     1921 ctggtggtgc gcgatgcgct ggaaggcgat cgcgcgcctg gtgatggtgc ggttactact
     1981 agccaattaa ttagcaaatg ccgcaaagtg gcgggctatt ttcatcgcag cattaaaggc
     2041 ggcaaaatgc tgcgcgataa acaggcggaa ctgagcattc cgcagcataa aattatgcag
     2101 gatgtggaaa cccgctggaa cagcacctat ctgatgctgg aacgcctggt ggaacagcag
     2161 aaagcgattc atgaaatggc gctggtgggc gaaattggca ttagcggccc tctgaacaaa
     2221 accgaatggg ataccattag ccagattctg gtggtgctga aaccgtttct ggaagcgacc
     2281 gaaagcctga gcgcgggcga tgcgttatta agccaggtga ttccggtggt gaaagaactg
     2341 gaaaacgaaa tggaaaaatt ccagggcatt gacgtgccgg gctggggcaa acctttaagc
     2401 cctgaagttc aagcgctggt gaaacagctg aaagaaggca ttaaaaaacg cctggatccg
     2461 ctgcgcagca gcaccgttca tgttttagcg ggtatgtgcg atccgcgtgt gaaaggtagc
     2521 gtgtgcagca gcaaaaccct ggatcattgg accgaagtgc tggtgaaccg cgtgcgcgaa
     2581 gcggaaggtc aacgtcgtgg tgatgttgaa gaaggcgatc cgctgagccg tgcgagcacc
     2641 cctaccacct cacaaagccc tcctcctcaa cctttaccga tgtgggcgaa aggtatggcg
     2701 agcatgctgg gcagccgtgg cactcgtcct catcatcaag cgggtagcgc ggaagcgagc
     2761 gttgcaacct atttagcgga agatgtggaa ccgctgcatt gcgatccgct ggcgtattgg
     2821 gcgacccgta gccaagtttg gcaagattta gcgaccgtgg cgcgtgaaca tctgagctgt
     2881 cctccgacca gcgttccgag cgaacgtgtt tttagcattg cgggcgatgt ggtgaccccg
     2941 catcgtacca gcttagatcc tggtttagtg gaacagctgg tgtttctgaa agtgaacctg
     3001 ccgctgctgg gctttccgaa actgcatgtg cagaccgaat aatgagcggc cgctcgagca
     3061 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     3121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     3361 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     3421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3841 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3901 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3961 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     4021 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4081 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4141 ttttggaggc ctaggctttt gcaaaaagct ccccgaaatg accgaccaag cgacgcccaa
     4201 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4261 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4321 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4441 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     4501 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4561 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4621 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4681 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4741 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4801 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4861 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4921 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4981 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5041 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5101 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5161 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5221 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5281 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5341 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5401 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5461 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5521 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5581 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5641 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5701 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5761 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5821 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5881 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5941 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6001 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6061 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6121 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6181 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6241 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6301 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6361 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     6421 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6481 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6541 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6601 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     6661 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     6721 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     6781 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     6841 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg tt
//
