LOCUS       Exported                5617 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..1767
                     /codon_start=1
                     /label=Senkusha1
                     /translation="MYIDGQADLRPDFRLSRSTVAKLIDVLRSPFDHGWGLEVEVLVFL
                     FWLAGATSYRVVSRGFSIPRSTVNDIVHRVSDKVLALKKCPIIFSKLDKLTTIAAGFQR
                     PAGSPASAKVVGSIDGCHVRIKPPGADAQCYFNRKLFYSIQLQAVCDHQGLFMDIFTGY
                     PGSVHDSRVLKNSPLYVQGLYPPEGYCILGDGGYPCMSRPVALITPYREQVRHMMAARF
                     NRHHAKARSVIERALGIMKTRWRAIFYKTLEVKPAFATKVIACCTILHNVCLRNGDSME
                     PSESQEKVVLLHPAADQTLDVIVTP"
     promoter        complement(1805..1823)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1849..2073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2119..2547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2561..2890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2741..2876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3056..3177
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3226..3242)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3250..3266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3274..3304)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3319..3340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3628..4216)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4387..5247)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5248..5352)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgt atatcgacgg ccaggcggat ttacgtccgg attttcgcct gagccgttca
      901 actgtggcga agctgattga tgttctgcgc agcccgtttg atcatggctg gggcctggaa
      961 gtggaggttc tggtgttcct gttttggtta gcaggcgcga ccagctatcg tgttgtgagc
     1021 cgcggtttta gcatacctcg cagcaccgtg aacgatattg tgcatcgcgt gagcgataaa
     1081 gtgctggcgc tgaaaaaatg ccctatcatc ttcagcaagc tggataagct gaccactatc
     1141 gcggcgggtt ttcaacgtcc tgcaggcagc cctgcaagcg caaaagttgt gggtagcatc
     1201 gatggctgcc atgtgcgcat taagcctcct ggcgcagatg cccaatgcta ttttaaccgc
     1261 aagctgttct atagcattca gctgcaggcc gtgtgtgatc atcagggcct gtttatggat
     1321 atcttcaccg gctatccggg cagcgttcat gatagccgcg tgctgaaaaa cagcccgctg
     1381 tatgtgcaag gcctgtaccc tcctgagggc tactgtatct taggcgatgg cggctatcct
     1441 tgtatgagcc gtccggtggc gttaataacc ccttaccgcg aacaagtgcg ccatatgatg
     1501 gcggcgcgtt tcaatcgtca tcacgcgaaa gcacgcagcg tgatagaacg tgcgctgggc
     1561 ataatgaaaa ctcgctggcg cgcgatcttt tataagaccc tggaagtgaa gcctgcgttt
     1621 gccaccaaag tgattgcgtg ctgcaccatt ctgcataacg tgtgcctgcg taacggtgac
     1681 agcatggagc ctagcgaaag ccaggagaaa gtggtgctgt tacaccctgc cgcggatcaa
     1741 accctggatg tgatcgtgac cccttaatga gcggccgctc gagcatgcat ctagagggcc
     1801 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     1861 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     1921 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     1981 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     2041 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     2101 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     2161 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     2221 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     2281 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     2341 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     2401 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2461 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     2521 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     2581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2821 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2881 cttttgcaaa aagctccccg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     2941 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     3001 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     3061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     3181 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     3241 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     3301 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3361 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3421 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3481 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3541 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3601 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3661 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3721 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3781 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3841 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3901 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3961 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4021 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4081 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4141 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4201 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4261 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4321 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4381 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4441 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4501 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4561 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4621 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4681 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4741 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4801 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4861 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4921 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4981 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     5041 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5101 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5161 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5221 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5281 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5341 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     5401 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5461 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     5521 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     5581 gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
