LOCUS       Exported                6679 bp ds-DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 23, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             847..2829
                     /codon_start=1
                     /label=Tigger17
                     /translation="MRRAAKNLSRPTRTFPAEVEQGDALPSCFSSHTVNKCPFRGLFSA
                     TFFAFLCFLLVISLFKMAPKRSAEVLSSVPKRKKAVMCLTEKIRVLDKLRSGMSYSAVG
                     REFNVNESTIRYIQKKEEEIRRSVREAAPESAKVTSIVRDEAMEKMEKRLNLWIHEMTT
                     DKKSVVDSIVVRLKAKEIYGHVTQGQENVKPFSASAGWLARFKRRYGVKNVKLAGEAGS
                     ADQEAAEEFLKYLLSVIQEKGYVEEQVFNADETGLFYKDVGKRTYITQMASKAPGFKSF
                     KDHATLLLCANAKGDFKCKPLMVHRAQNPRALKGKNLNRMPVHWRWNKKAWMASEIFWD
                     WFHNCFIPEVERYLQGENLAFRVLLILDNAPVHCREELENAHPNVEVLFMPPNTASLIQ
                     PLGQGIIKAFKAHYTRELYSKAFEALDANKETTMMDYWKSVTIRSVIDYVGTAWDSIKQ
                     ATIDNCWENVWPDCVKNLKSEGVTESIKNSVKNIMRIARQISGEGFDDMKEEDVEEILA
                     EKAVEPTNEDLDEMAKQGIGVGDDEDGDESQPKTPRIVPLTAAKISEWNSALEKIFSDM
                     EECDPMLDRSLKFKRLTSTAFIPYAETLKDLRRKAKQTRLTQFFEPVWEGKSPTPSTSY
                     ESQTPEVELPDVDMPPSPTSSSSAE"
     promoter        complement(2867..2885)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    2911..3135
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3181..3609
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3623..3952
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3803..3938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4118..4239
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4288..4304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4312..4328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4336..4366)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4381..4402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4690..5278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5449..6309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6310..6414)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacgtc cgcgcagggc aatagcagag ctctctggct
      721 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      781 ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct ggaattcgcc
      841 gccaccatgc gtcgtgcggc caaaaatctg tcacgtccga cccgtacctt tccggccgag
      901 gttgaacagg gtgatgcgct gcctagctgc tttagcagcc acaccgtgaa caaatgcccg
      961 ttccgcggtt tatttagcgc caccttcttc gcgtttctgt gcttcctgct ggtgattagc
     1021 ctgtttaaga tggcgcctaa gcgcagcgcg gaagttttaa gcagcgtgcc taagcgcaaa
     1081 aaagcggtga tgtgcctgac cgaaaaaatc cgcgtgctgg ataaattacg cagcggcatg
     1141 agctattcag cagttggtcg cgagttcaac gtgaacgaaa gcaccattcg ctacatccag
     1201 aaaaaggagg aagaaattcg ccgcagcgtt cgtgaagcag cacctgaaag cgcgaaagtg
     1261 accagcattg tgcgcgacga agcgatggag aaaatggaaa aacgcctgaa cctgtggatt
     1321 cacgaaatga ccaccgacaa aaaaagcgtg gtggatagca ttgtggtgcg cctgaaagcg
     1381 aaagaaattt acggccatgt gacccagggc caggaaaacg tgaaaccgtt tagcgcaagc
     1441 gcaggttggc tggcgcgttt taaacgccgc tatggcgtga aaaacgtgaa actggccggt
     1501 gaagcaggta gcgcggatca agaagcggcc gaagaatttc tgaaatatct gctgagcgtg
     1561 attcaggaaa aaggctacgt ggaggagcaa gtgttcaacg cggacgaaac cggcctgttc
     1621 tataaagatg tgggcaagcg cacctacatt acccagatgg cgtcaaaagc gccgggcttc
     1681 aaaagcttca aagatcatgc caccctgctg ttatgtgcga acgccaaggg cgacttcaag
     1741 tgcaaaccgc tgatggtgca tcgcgcccaa aatcctcgtg ccctgaaagg caaaaacctg
     1801 aaccgcatgc cggttcattg gcgctggaac aaaaaagcct ggatggccag cgaaattttc
     1861 tgggactggt ttcataactg cttcatccct gaagtggaac gctacctgca gggtgagaac
     1921 ctggcatttc gcgtgctgct gattctggat aacgcgcctg tgcactgtcg tgaggaactg
     1981 gaaaacgcgc acccgaacgt ggaagtgctg tttatgcctc cgaacaccgc gagcttaatt
     2041 caaccgctgg gccagggcat tattaaggcc tttaaggcgc attatacccg cgaactgtat
     2101 agcaaagcct ttgaggcgct ggatgcgaac aaggaaacca ccatgatgga ttactggaag
     2161 agcgtgacca ttcgcagcgt gatcgactat gttggcaccg cctgggattc aattaagcag
     2221 gcgaccattg ataactgctg ggaaaacgtg tggcctgact gcgtgaaaaa cctgaaaagc
     2281 gaaggcgtga ccgaaagcat taaaaacagc gtgaagaaca ttatgcgcat cgcgcgccaa
     2341 atcagcggtg aaggcttcga cgacatgaaa gaagaagacg tggaagaaat tctggccgaa
     2401 aaggcggttg agcctaccaa cgaagatctg gatgagatgg cgaaacaggg tatcggcgtg
     2461 ggcgatgatg aagatggcga cgagagccaa cctaaaaccc ctcgcattgt gcctctgacc
     2521 gccgcaaaga ttagcgagtg gaacagcgcc ctggaaaaga tctttagcga tatggaagag
     2581 tgcgatccta tgctggatcg cagcctgaag ttcaagcgct taaccagcac cgcctttatt
     2641 ccgtatgccg aaaccctgaa agatctgcgc cgcaaagcga aacaaactcg cctgacccag
     2701 ttttttgagc ctgtgtggga gggtaaaagc cctaccccta gcaccagcta cgaaagccag
     2761 accccggaag ttgaactgcc ggatgtggat atgcctccga gccctacttc atcaagcagc
     2821 gcggaataat gagcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc
     2881 taaatgctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     2941 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3001 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3061 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3121 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     3181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     3541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3601 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3721 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3781 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     3901 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     3961 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     4021 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     4081 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     4141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     4261 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     4321 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     4381 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     4441 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4501 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4561 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4621 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4681 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4741 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4801 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4861 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4921 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4981 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5041 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5101 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5161 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5221 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5281 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5341 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5401 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5461 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5521 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5581 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5641 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5701 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5761 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5821 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5881 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5941 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6001 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6061 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6121 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6181 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6241 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6301 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6361 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6421 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     6481 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     6541 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     6601 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     6661 cgggccagat atacgcgtt
//
